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143KB - NZQA
143KB - NZQA

... their understanding. It was clear that many candidates were well prepared for the examination. They made good use of the resources such as exemplar materials, past year exam questions and schedules, including assessment specifications which are all available on the NZQA website. Many candidates limi ...
Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter
Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter

... In Fig. 3, the 1523 bp F6 promoter fragment was employed to determine any effect of other Scd enzyme substrate fatty acids on the bovine Scd promoter. Thirty micromolar concentrations of different fatty acids were used to treat the cells in Figs. 3A and B. As seen in Fig. 3A, there was no effect of the ...
Parental Legacy Determines Methylation and Expression of an
Parental Legacy Determines Methylation and Expression of an

... other strains of mice inheriting RSV fusion genes (Overbeek, et al., 1986). Thus, the tissue specificity is likely to be a property of the RSV LTR rather than the site of integration, though this is certainly not proven. With respect to the physiologic effect of transgene expression, preliminary ind ...
Prediction of Effective genome size in metagenomics samples
Prediction of Effective genome size in metagenomics samples

... Classical approaches for estimating genome size – two examples for possible errors ...
Learning About DNA
Learning About DNA

... scientists developed a tool to make things look larger than their actual size. Microscopes use lenses (curved pieces of glass) to bend light rays in order to make an enlarged image. The first compound microscope was invented around 1590 by two Dutch eyeglass makers, Hans and Zacharias Janssen. Their ...
computation for chIP-seq and rNA-seq studies
computation for chIP-seq and rNA-seq studies

... on (i) achieving sufficient enrichment of factor-bound chromatin relative to nonspecific chromatin background and (ii) obtaining sufficient enriched chromatin so that each sequence obtained is from a different founder molecule in the ChIP reaction (in other words, that the molecular library has adeq ...
Application of rpoB sequence similarity analysis, REP‐PCR and
Application of rpoB sequence similarity analysis, REP‐PCR and

... gene. The same difference was designated by Zeigler (2005) during comparison of 16S rRNA and recN deriving phylogenies because of the mutational saturation of the later gene. Although bootstrap values were lower for the rpoB tree, both phylogenetic analyses shared satisfactory bootstrap support. In ...
Recent retrotransposition events have not affected
Recent retrotransposition events have not affected

... expression divergence between human and chimpanzee. (However, TEs may still contribute substantially to within-species variation). We do not find evidence for a TE-induced increase in transcript diversity. Excess of upstream insertions can be explained by neutral insertion bias. ...
Massachusetts Institute of Technology
Massachusetts Institute of Technology

... b. Can you find any examples of “trapped” non-ancestral material (colored white) between blocks of ancestral material? c. Try to press the ‘trees’ tab. Here it is possible to study each of the different trees over the sequence. How many different trees are there and how does this relate to the numb ...
Chromosomes Identification
Chromosomes Identification

... Can distinguish chromosomes by “painting ” – using DNA hybridization + fluorescent probes – during mitosis ...
IMSR File Format
IMSR File Format

... A mutation induced by treatment with a chemical mutagen, for example, ENU (ethyl nitrosourea) or chlorambucil. Chromosome A structural unit within a eukaryotic nucleus that carries genes. A chromosome consists of a long, continuous strand of DNA and associated proteins. Chromosomal Aberration (CH) A ...
Organism # of Gamete # of Zygote # of Pairs of Zygote
Organism # of Gamete # of Zygote # of Pairs of Zygote

... tom cat is mated with an Angora female. She has a litter of 8 kittens, 6 short-haired and 2 with long hair. If you mated these same cats 4 more times, getting a total of 40 offspring, would you expect the results to be closer to the expected ratio? What was the genotype of the torn cat? 15. Anoather ...
Copies of Student Information pages
Copies of Student Information pages

... The traits you have are determined by the genes in the chromosomes you inherit from your parents. A gene is a specific place on a chromosome that determines a trait (characteristic). Every trait is controlled by at least one gene from Mom and at least one gene from Dad, thus it takes at least one g ...
+ 2 subjective question bank reproduction
+ 2 subjective question bank reproduction

... Ans. It is a procedure in which amniotic fluid is taken from amniotic sac of the foetus to diagnose various chromosomal and genetic disorders. During this test sex of the baby is also revealed. This promotes the practice of female foeticide. Hence, it is justified to ban the process to check female ...
SUPPLEMENTARY DATA
SUPPLEMENTARY DATA

... Growth media, drug treatment and yeast strains. Yeast strains used in this study are listed in Table SI and plasmids in Table SII. The yeast cells were grown either in synthetic growth media lacking the nutrients indicated or in rich media. ...
Two enhancer regions in the mouse En-2 locus
Two enhancer regions in the mouse En-2 locus

... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
genetic mapping and dna sequencing of the loblolly pine genome
genetic mapping and dna sequencing of the loblolly pine genome

... WLLLIAMS1991; DEVEYet al. 1991). At this time RFLPs were the "state-of-art" marker. PCR-based markers had not yet been developed. RFLP probes derived from cDNA and genomic DNA were evaluated for use in loblolly pine. It was determined that as high, or higher, levels of polymorphism could be detected ...
Oral squamous cell carcinoma (OSCC) – molecular, viral and
Oral squamous cell carcinoma (OSCC) – molecular, viral and

... a strong association between the presence of HPV DNA – specifically HPV16 and OSCC. This meta-analysis showed that HPV significantly increases the risk for OSCC, compared with controls (OR 3.98; 95% CI: 2.62–6.02). HPVs specifically target the undifferentiated proliferative basal cells of epithelial ...
Electrophoresis Systems for Nucleic Acids
Electrophoresis Systems for Nucleic Acids

... (sample) is added to agarose gel and electrophoresed in buffer solution for a regulated time, the level of migration shown is in accordance with the size of nucleic acid due to the molecular sieving effect of gel. When this is dyed with ethidium bromide, etc. and detected, the electrophoresis patter ...
C2005/F2401 `07 -- Lecture 19 -- Last Edited
C2005/F2401 `07 -- Lecture 19 -- Last Edited

... 1. Chromosomes. DNA (+ associated proteins) visible in microscope as individual structures called chromosomes. DNA tightly coiled, easy to distribute but not accessible to enzymes of replic. and transc. (condensed > 10,000 X). Individual balls of string (in this state) vs unwound, tangled mess (betw ...
Pan-genomics: Unmasking the gene diversity hidden in the bacteria
Pan-genomics: Unmasking the gene diversity hidden in the bacteria

... The in-depth study of the core genome sheds light over relevant evolutionary questions, like what are the conserved genes across a taxonomic range, its cut-off similarity values and what are the functional gene constrains of this conservancy. Based on the central dogma of molecular biology one would ...
Functional constraints and frequency of deleterious mutations in
Functional constraints and frequency of deleterious mutations in

... in the genome and to quantify the fraction of deleterious mutations is to search for segments of the genome having lower between-species levels of divergence than the average for the genome or than a linked putatively neutral sequence (14). Previous attempts to quantify the fraction of conserved nuc ...
Solving Multiple Sequence Alignment Problems using Various E
Solving Multiple Sequence Alignment Problems using Various E

... sequence). However, this is based on the assumption that the combined monomers evenly spaced along the single dimension of the molecule's primary structure. From now on, we will refer to an alignment of two DNA sequences. Every element in a trace is either a match or a gap. Where a residue in one of ...
Genes Involved in the Seminoma Testicular Cancer
Genes Involved in the Seminoma Testicular Cancer

... difference allows infer changes as a result of treatment, disease or other causes. Because, this task is widely used in fields such as pharmaceuticals, in view of facilitating the development of medicines to the suppression or activation of genes related to various types of cancer. As a result, in t ...
Substitution Rates in a New Silene latifolia Sex
Substitution Rates in a New Silene latifolia Sex

... and c2B12–2 primers, and the smaller fragment corresponded to SlssX sequence. Segregation analysis in the family 1 (seven male and eight female F1 progeny) also confirmed that the larger (4.5 kb long) band is Y linked. The X linkage of the smaller (;4 kb long) PCR product was confirmed by partial di ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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