Leapfrogging: primordial germ cell transplantation
... sexual maturity were assayed for germline transmission of tyr mutant alleles. F0 animals were crossed with animals from a homozygous albino (tyr −/−) population that we previously established. Since the albino phenotype is only observed in homozygous tyr-deficient (null) offspring, scoring of the F1 ...
... sexual maturity were assayed for germline transmission of tyr mutant alleles. F0 animals were crossed with animals from a homozygous albino (tyr −/−) population that we previously established. Since the albino phenotype is only observed in homozygous tyr-deficient (null) offspring, scoring of the F1 ...
Root organization and gene expression patterns
... Poaceae) there are three initial layers, the plerome (vascular cylinder), the periblem/dermatogen (cortex and epidermis) and the calyptrogen (root cap). The most common closed type in dicotyledonous plants also has three layers but in a different pattern, the plerome (vascular cylinder), the perible ...
... Poaceae) there are three initial layers, the plerome (vascular cylinder), the periblem/dermatogen (cortex and epidermis) and the calyptrogen (root cap). The most common closed type in dicotyledonous plants also has three layers but in a different pattern, the plerome (vascular cylinder), the perible ...
transcription factor binding site
... Eland, an efficient and fast aligner for short reads that was developed by Illumina and is the default aligner on that platform; Mapping and Assembly with Qualities (MAQ)58, a widely used aligner with a more exhaustive algorithm and excellent capabilities for detecting SNPs; and Bowtie59, an extreme ...
... Eland, an efficient and fast aligner for short reads that was developed by Illumina and is the default aligner on that platform; Mapping and Assembly with Qualities (MAQ)58, a widely used aligner with a more exhaustive algorithm and excellent capabilities for detecting SNPs; and Bowtie59, an extreme ...
osb week10 lab
... Question 3: Develop a table describing the possible genotypes and their phenotypes that could result from a cross between a tortoiseshell and a black cat. Assume both cats are homozygous BB. Complex Traits Complex traits are controlled by more than one gene. Of course, cat color is controlled by mul ...
... Question 3: Develop a table describing the possible genotypes and their phenotypes that could result from a cross between a tortoiseshell and a black cat. Assume both cats are homozygous BB. Complex Traits Complex traits are controlled by more than one gene. Of course, cat color is controlled by mul ...
Deletions, Duplications and Inversions ppt
... An inversion in theory may not be detrimental since no genes are gained or lost due to the inversion Gene function could be affected if the break occurs in the middle of a gene or in its promoter region Gene function could be affected by moving into or away from a region of heterochromatin ...
... An inversion in theory may not be detrimental since no genes are gained or lost due to the inversion Gene function could be affected if the break occurs in the middle of a gene or in its promoter region Gene function could be affected by moving into or away from a region of heterochromatin ...
Doubling Down on Genomes: Polyploidy and Crop Plants
... populations are unlikely to increase fitness, leading to the suggestion that nascent allopolyploids need to overcome an initial fitness cost associated with genomic aberrations. Genomic plasticity in polyploidy plants is not restricted to karyotypic alterations. Another large-scale alteration common ...
... populations are unlikely to increase fitness, leading to the suggestion that nascent allopolyploids need to overcome an initial fitness cost associated with genomic aberrations. Genomic plasticity in polyploidy plants is not restricted to karyotypic alterations. Another large-scale alteration common ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
... therefore a measure of the stressed state of the DNA, due to both its crowding and bending. It has recently been shown that this force can be determined by experiments in which osmotic pressure is employed to inhibit the ejection (6). The results of these studies are consistent with the predictions ...
Copy number variants and genetic traits: closer to the resolution of
... polygenic) diseases and variation in the phenotypic expression of aneuploidies and sporadic traits, and might represent a major factor in the aetiology of complex, multifactorial traits. For these reasons, an effort should be made to discover all common and rare copy number variants (CNVs) in the hu ...
... polygenic) diseases and variation in the phenotypic expression of aneuploidies and sporadic traits, and might represent a major factor in the aetiology of complex, multifactorial traits. For these reasons, an effort should be made to discover all common and rare copy number variants (CNVs) in the hu ...
Introduction - bei DuEPublico
... 1) growth signal autonomy 2) evasion of apoptosis 3) insensitivity to antigrowth signals 4) sustained angiogenesis 5) limitless replicative potential and 6) capacity to invade tissue and grow at metastatic sites The number of mutations required to gain these abilities varies. For example, in some ce ...
... 1) growth signal autonomy 2) evasion of apoptosis 3) insensitivity to antigrowth signals 4) sustained angiogenesis 5) limitless replicative potential and 6) capacity to invade tissue and grow at metastatic sites The number of mutations required to gain these abilities varies. For example, in some ce ...
Rare genomic changes and mitochondrial sequences
... characters for making phylogenetic inferences, mitochondrial genomes provide a rich source of sequences for analysis. Typical metazoan mitochondria encode the same 37 genes, consisting of 13 protein-coding genes, 22 tRNA genes, and 2 ribosomal RNA genes. Therefore, it is clear that each of these gen ...
... characters for making phylogenetic inferences, mitochondrial genomes provide a rich source of sequences for analysis. Typical metazoan mitochondria encode the same 37 genes, consisting of 13 protein-coding genes, 22 tRNA genes, and 2 ribosomal RNA genes. Therefore, it is clear that each of these gen ...
Life 9e - Garvness
... DNA yields no surprises. However, it is found that a codon for this life-form is just two bases in length. How many different amino acids could this organism be composed of? ...
... DNA yields no surprises. However, it is found that a codon for this life-form is just two bases in length. How many different amino acids could this organism be composed of? ...
$doc.title
... The code in the class DNABenchMark can be used to benchmark the cutAndSplice method. The code given to you will pop-‐up a file-‐dialog box — when run you can use this to nav ...
... The code in the class DNABenchMark can be used to benchmark the cutAndSplice method. The code given to you will pop-‐up a file-‐dialog box — when run you can use this to nav ...
Structure, expression and chromosomal location of the Oct
... family show distinct expression patterns during embryonic development (He et al., 1989; Sch61er et al., 1989a). Octamer-binding transcription factor 4 ( O c t - 4 ) is a recently described POU transcription factor that is expressed early in the preimplantation embryo, and thus may regulate initial e ...
... family show distinct expression patterns during embryonic development (He et al., 1989; Sch61er et al., 1989a). Octamer-binding transcription factor 4 ( O c t - 4 ) is a recently described POU transcription factor that is expressed early in the preimplantation embryo, and thus may regulate initial e ...
- Wiley Online Library
... alga Acetabularia; the question arises what advantage accrues to fungi from multiple nuclei in same cytoplasm, when cells in more complex forms have just one nucleus per cell? Serna and Stadler found that the rate of spore germination in N. crassa was not related to nuclear number [16]. Therefore, i ...
... alga Acetabularia; the question arises what advantage accrues to fungi from multiple nuclei in same cytoplasm, when cells in more complex forms have just one nucleus per cell? Serna and Stadler found that the rate of spore germination in N. crassa was not related to nuclear number [16]. Therefore, i ...
Severe Perinatal Thrombosis in Double and Triple Heterozygous
... protein C activity level of 4 U/dL [NR, 24 to 38 weeks gestation, fetuses, and neonates, 8.1 to 22.91;’ and a borderline normal protein C antigen level of 17 U/dL [NR day 1 (full-term), 17 to 531. Total and free protein S antigen levels were found to be 14 U/dL [NR day 1 (full-term), 17 to 531 and 2 ...
... protein C activity level of 4 U/dL [NR, 24 to 38 weeks gestation, fetuses, and neonates, 8.1 to 22.91;’ and a borderline normal protein C antigen level of 17 U/dL [NR day 1 (full-term), 17 to 531. Total and free protein S antigen levels were found to be 14 U/dL [NR day 1 (full-term), 17 to 531 and 2 ...
Eds., Y. Murakami, K. Nakayama, S.-I. Kitamura, H. Iwata and... © by TERRAPUB, 2008.
... The TC-resistant Vibrio and L. garvieae containing tet(M) gene were used for transfer experiment of TC resistance to E. coli and E. faecalis by filter-mating and filter-separating methods. As shown in Table 1, transformation rate in filter mating of E. coli was 10 –3 when Vibrio was donor, whereas t ...
... The TC-resistant Vibrio and L. garvieae containing tet(M) gene were used for transfer experiment of TC resistance to E. coli and E. faecalis by filter-mating and filter-separating methods. As shown in Table 1, transformation rate in filter mating of E. coli was 10 –3 when Vibrio was donor, whereas t ...
Legends for Tables S1 to S3 and Figures S1 to S5
... primary MM patient samples and sensitivity to venetoclax ex vivo. Expression of BCL2 (BCL-2), BCL2L1 (BCL-XL) and MCL1 (MCL-1) mRNA in purified CD138-positive primary multiple myeloma patient samples were determined by TaqMan-based PCR as described in Supplemental Materials and Methods. mRNA levels ...
... primary MM patient samples and sensitivity to venetoclax ex vivo. Expression of BCL2 (BCL-2), BCL2L1 (BCL-XL) and MCL1 (MCL-1) mRNA in purified CD138-positive primary multiple myeloma patient samples were determined by TaqMan-based PCR as described in Supplemental Materials and Methods. mRNA levels ...
Simultaneous mutation scanning for gross deletions
... cDNA.20 All of the DNA-based methods described so far screen either for large deletions and duplications, or for point mutations, but not both. RNA-based methods are capable of detecting all types of mutation but do not detect all gross duplications, and as mentioned for PTT, their major disadvantag ...
... cDNA.20 All of the DNA-based methods described so far screen either for large deletions and duplications, or for point mutations, but not both. RNA-based methods are capable of detecting all types of mutation but do not detect all gross duplications, and as mentioned for PTT, their major disadvantag ...
Document
... Interferon gamma (IFNγ), a cytokine involved in multiple aspects of iron metabolism and the immune response , has been shown to increase frataxin levels in both cell and animal models of FRDA It has been reported that in vivo treatment of YG8R mice with IFNγ enhances both locomotor activity and moto ...
... Interferon gamma (IFNγ), a cytokine involved in multiple aspects of iron metabolism and the immune response , has been shown to increase frataxin levels in both cell and animal models of FRDA It has been reported that in vivo treatment of YG8R mice with IFNγ enhances both locomotor activity and moto ...
Phylogenetic Relationships Among Ascomycetes: Evidence from an
... pCR2.1 plasmid vector (Invitrogen Inc., Carlsbad, Calif.). A number of clones for each RPB2 fragment were subjected to automatic sequencing (ABI PRISM Dye Terminator Cycle Sequencing and ABI PRISM Sequencer model 377, Perkin-Elmer). Fungal-specific primers were then designed based on the RPB2 sequen ...
... pCR2.1 plasmid vector (Invitrogen Inc., Carlsbad, Calif.). A number of clones for each RPB2 fragment were subjected to automatic sequencing (ABI PRISM Dye Terminator Cycle Sequencing and ABI PRISM Sequencer model 377, Perkin-Elmer). Fungal-specific primers were then designed based on the RPB2 sequen ...
allele 2 Proteins made from allele 1 chromosome Proteins made
... If a mutation occurs in the DNA of an allele, the protein made may have an incorrect structure and not work properly. Alternatively, some mutations can result in no protein being made at all. The tasks below will make you explore the differences between normal and mutated proteins and how changes in ...
... If a mutation occurs in the DNA of an allele, the protein made may have an incorrect structure and not work properly. Alternatively, some mutations can result in no protein being made at all. The tasks below will make you explore the differences between normal and mutated proteins and how changes in ...
Sequence comparison of aflR from different Aspergillus species
... Cys6 Zn2 DNA-binding domain and C-terminal transcription activation domain typical of GAL4-type fungal and yeast transcription factors. AflR binds to the partially palindromic consensus sequence 50 -TCGN5 CGR-30 found in promoters of most of the aflatoxin biosynthesis genes (Chang et al., 1995; Ehrlic ...
... Cys6 Zn2 DNA-binding domain and C-terminal transcription activation domain typical of GAL4-type fungal and yeast transcription factors. AflR binds to the partially palindromic consensus sequence 50 -TCGN5 CGR-30 found in promoters of most of the aflatoxin biosynthesis genes (Chang et al., 1995; Ehrlic ...
Not now, but soon: Justification for continued research on CRISPR
... organism in the blink of an eye. The name of the technology is CRISPR-Cas9, and it is attracting attention from scientists and researchers around the world as the newest and most efficient piece of genome editing technology available. CRISPR-Cas9 can create double- stranded breaks in DNA at precise ...
... organism in the blink of an eye. The name of the technology is CRISPR-Cas9, and it is attracting attention from scientists and researchers around the world as the newest and most efficient piece of genome editing technology available. CRISPR-Cas9 can create double- stranded breaks in DNA at precise ...
Evidence for massive gene exchange between archaeal and
... makes it possible to perform systematic, genome-scale comparisons that aim to delineate the genomic complement of a particular phenotype. Recently, the first genome of a hyperthermophilic bacterium, Aquifex aeolicus, has been sequenced1. Previous studies based on rRNA and aminoacyl-tRNA analysis had ...
... makes it possible to perform systematic, genome-scale comparisons that aim to delineate the genomic complement of a particular phenotype. Recently, the first genome of a hyperthermophilic bacterium, Aquifex aeolicus, has been sequenced1. Previous studies based on rRNA and aminoacyl-tRNA analysis had ...
Molecular Evolution of the CMT1A-REP Region: A Human
... may have been present in the genomes of multiple primates and subsequently deleted in all but humans and chimpanzees. To address this possibility, homologous sequences immediately flanking the centromeric and telomeric ends of the proximal REP in humans were examined in a series of primates (see fig ...
... may have been present in the genomes of multiple primates and subsequently deleted in all but humans and chimpanzees. To address this possibility, homologous sequences immediately flanking the centromeric and telomeric ends of the proximal REP in humans were examined in a series of primates (see fig ...