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Review: To bud until death: The genetics of ageing in the yeast
Review: To bud until death: The genetics of ageing in the yeast

... Defining ageing in budding yeast is possible only because of the asymmetry of its cell cycle. The mother cell is usually larger than its newly budded daughter and can therefore be identified at the end of each cell division. This allowed Mortimer and Johnston2' in 1959 to show for the first time tha ...
Structure, expression differentiation and evolution of duplicated fiber
Structure, expression differentiation and evolution of duplicated fiber

... species, representing descendants from the A-genome and D-genome donors at the time of polyploidy formation. The goals of this study were to: 1) better understand the genetic basis of cotton fiber development, 2) identify the structural difference of duplicated genes, and 3) reveal the expression an ...
West Nile Neuroinvasive Disease Case Definitions Reproduced
West Nile Neuroinvasive Disease Case Definitions Reproduced

... Case genotypes were prepared from the passing exome sequencing calls of all 44 WNVE subjects. In silico control genotypes were derived from 379 subjects of European ancestry included in the 1000 Genomes Project phase 1 v3.20101123 release. Case and control panels were independently filtered to exclu ...
extensions
extensions

... •  Traits that occur in only one of the two sexes –  Responsible for sexual dimorphism ...
extensions
extensions

... • Three alleles affect this sugar addition – The i allele encodes a defective enzyme that can’t add sugars • The carbohydrate tree is short (called H antigen) – IA encodes a form of the enzyme that can add the sugar N-acetylgalactosamine to the carbohydrate tree (makes A antigen) – IB encodes a for ...
Invited Review: How sleep deprivation affects gene expression in
Invited Review: How sleep deprivation affects gene expression in

... upregulated after both 3 and 8 h of wakefulness and sleep deprivation), other IEGs/transcription factors are only induced after sustained (8 h) periods of sleep loss (7). They include CHOP, IER5, NGFI-B, N-ras, and Stat3. Many of these genes may play a role in promoting the transcription of “late” g ...
VegT activates Bix4 to specify endodermal
VegT activates Bix4 to specify endodermal

... (Tada et al., 1998). Bix1-Bix4 were originally isolated as targets of the T-box gene Xbra, which is expressed at the onset of gastrulation in the prospective mesoderm of the Xenopus embryo (Smith et al., 1991). However, like the related genes Mix.1 (Rosa, 1989), Mix.2 (Vize, 1996) and Milk (Ecochard ...
Th17/Treg ratio derived using DNA methylation asthmatic response
Th17/Treg ratio derived using DNA methylation asthmatic response

... shown to be higher in subjects with allergic asthma compared to healthy controls [3,4]. The percentage of Th17 cells and IL-17 levels in peripheral blood have been shown to be significantly elevated 24 hours after allergen challenge in dual responders compared to early responders or healthy controls ...
Transgenic Tobacco Plants with Bacillus thuringiensis 8
Transgenic Tobacco Plants with Bacillus thuringiensis 8

... which leads to osmotic imbalance and cell lysis (Hofmann et ai., 1988; Slatin et ai., 1990; English et al., 1991; English and Slatin, 1992). Among insecticidal protein genes, lepidopteran specific genes, cryJA class, are mostly well characterized (Adang et al., 1985; Thorne et al., 1986; Schnepf et ...
Interaction of Sesbania Mosaic Virus Movement Protein
Interaction of Sesbania Mosaic Virus Movement Protein

... and dynamic process which involves functional contribution from many proteins of viral and host origin [2]. A number of RNA viruses have been identified which require an MP and two or more additional proteins for effective cell-to-cell spread of their genetic material. For example, in the case of po ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
MGF 360-17R Missing
MGF 360-17R Missing

Use of a novel cassette to label phenotypically a cryptic plasmid of
Use of a novel cassette to label phenotypically a cryptic plasmid of

... efficiently if transcribed in B. subtilis (Zukowski et al., 1983). Since the xylE gene comes from the centre of an operon it lacks its own promoter and so to achieve efficient expression, heterologous promoters tac and trp from E. coli, and a S P 0 2 Bacillus phage promoter, all of which are known t ...
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics

... 718 amino acids; 82.2 kDa. ...
Molecular Mechanisms of Developmental Review
Molecular Mechanisms of Developmental Review

... al., 1997). The 3⬘-UTR of the lin-28 mRNA contains a single potential lin-4 binding site, and deletion of this sequence results in a gain-of-function lin-28 mutant phenotype as well as persistent LIN-28 protein. Interestingly, the number of potential lin-4 binding sites correlates with the timing of ...
Brooker Chapter 22
Brooker Chapter 22

... high level of fluorescence Those that are affected with TSD produce little fluorescence Heterozygotes produce intermediate levels of fluorescence Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
DNA methylation profiling identifies epigenetic dysregulation in
DNA methylation profiling identifies epigenetic dysregulation in

... methylation status of 27 578 CpG sites corresponding to 14 475 consensus coding sequences and well-known cancer genes (Bibikova et al, 2009). Although the Infinium methylation assay is not a genome-wide DNA methylation technology, it is a useful screening tool that is sensitive, specific and highly re ...
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a
Acute stress and hippocampal histone H3 lysine 9 trimethylation, a

... DNA transposons showed 25-fold, satellite repeats showed 10fold, and noncoding RNA loci showed 39-fold differentials, respectively (Fig. 3B). Representative stress-up and -down regions are displayed in Fig. 3 C and D, respectively. To further assess if the stress-induced increase in H3K9me3 showed a ...
Peer Review 1
Peer Review 1

... Assuming the result of this procedure is that tGLI1 is present in at least some of the tumours, I would proceed with the following experiment. The second experiment (the focus of this project) would be an “add something” experiment. Specifically, I would increase SUFU expression using a knocked-in g ...
Inactivation of Antibiotics and the Dissemination of
Inactivation of Antibiotics and the Dissemination of

... 13-1actam use. In some cases, increased resistance resuhs from increased expression of the [3-1actamase through an up-promoter mutation (10), or chromosomal 13-1actamase genes can be overexpressed in highly resistant strains as a result of changes in transcriptional regulation f i t ) . The fact tha ...
and Light-Chain Variable-Region Gene Families
and Light-Chain Variable-Region Gene Families

... VH genes. The Vl sequences from humans belong to several groups, whereas the sequences from chickens, rabbits, cattle, and sheep are restricted to one Vl group. The two remaining species, mice and horses, show a somewhat unusual pattern. In mice, in contrast to numerous VH genes (see fig. 1), there ...
Genes Identified by Visible Mutant Phenotypes Show Increased Bias
Genes Identified by Visible Mutant Phenotypes Show Increased Bias

... between the two subgenomes of maize roughly mirrors that of all syntenically retained genes supported by full length cDNA evidence. Figure 4 plots these data for all 34,579 genes supported by full length cDNA evidence, the 468 genes of the classical gene list, and the subset of 102 genes on the clas ...
General background text Pharmacogenetics
General background text Pharmacogenetics

... There is also great variation in metabolic capacity within the IM and EM groups. The PM phenotype particularly leads to a strong increase in cellular concentration of the active metabolites (thioguanine nucleotides), which almost always leads to serious side effects such as bone marrow depression at ...
pdf
pdf

... of nirK genes than do primers nirK1F and nirK5R due to the occurrence of three nucleotides within the nirK1F target region of certain sequences which are unaccounted for in that primer sequence (Fig. 1). Conditions for amplifying nirK using primers Cunir3 and Cunir4 were optimized with genomic DNA e ...
form/activity - Science of Security
form/activity - Science of Security

... • Focus of most research and innovation in both science and engineering • Widely viewed as most important ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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