HLRCC Science
... Many details were left out of the above explanation so that the understanding of how DNA codes for mRNA, and how mRNA, in turn, codes for the sequence of amino acids that makes up a protein would be easier to comprehend. Just a few of these details will be added here. There are actually several diff ...
... Many details were left out of the above explanation so that the understanding of how DNA codes for mRNA, and how mRNA, in turn, codes for the sequence of amino acids that makes up a protein would be easier to comprehend. Just a few of these details will be added here. There are actually several diff ...
Regulatory sequences of H19 and IGF2 genes in DNA‐based
... immune responses directed against adenovirus proteins, and limits their ability to be administered iteratively. Based on early studies from our group and others, transcriptional regulatory sequences of the H19 gene have emerged as candidates for cancer gene therapy. H19 is a paternally imprinted, on ...
... immune responses directed against adenovirus proteins, and limits their ability to be administered iteratively. Based on early studies from our group and others, transcriptional regulatory sequences of the H19 gene have emerged as candidates for cancer gene therapy. H19 is a paternally imprinted, on ...
MicroRNAs as Oncogenes and Tumor Suppressors
... specimens has been tremendously successful in building a molecular taxonomy of cancer that can be used to identify new drugs, make a prognosis, and predict patterns of drug resistance. However, cellular behavior is also governed by translational and posttranslational control mechanisms that are not ...
... specimens has been tremendously successful in building a molecular taxonomy of cancer that can be used to identify new drugs, make a prognosis, and predict patterns of drug resistance. However, cellular behavior is also governed by translational and posttranslational control mechanisms that are not ...
D. melanogaster - GEP Community Server
... RAMPAGE = RNA Annotation and Mapping of Promoters for Analysis of Gene Expression CAGE only allows sequencing of short sequence tags (~27 bp) near the 5’ cap Ambiguous read mapping to large parts of the genome ...
... RAMPAGE = RNA Annotation and Mapping of Promoters for Analysis of Gene Expression CAGE only allows sequencing of short sequence tags (~27 bp) near the 5’ cap Ambiguous read mapping to large parts of the genome ...
Barley Cbf3 Gene Identification, Expression Pattern, and Map Location
... We found several putative regulatory elements, including ABA response elements and DRE/CRTs, in the upstream regions of the barley (Hordeum vulgare) Dhn genes (Choi et al., 1999). The presence of these regulatory elements is consistent with Dhn gene expression patterns under dehydration, low tempera ...
... We found several putative regulatory elements, including ABA response elements and DRE/CRTs, in the upstream regions of the barley (Hordeum vulgare) Dhn genes (Choi et al., 1999). The presence of these regulatory elements is consistent with Dhn gene expression patterns under dehydration, low tempera ...
Sequences 5` to Translation Start Regulate
... the SSL/977 gene 25-fold irrespective of its orientation (Figure 4). This effect parallels the increase in expression observed after the addition of the -1300 to -178 SSL/307 fragment. The addition of 110 bp of ocs coding region to the SSL/97 7 promoter (construction 9) did not alter significantly t ...
... the SSL/977 gene 25-fold irrespective of its orientation (Figure 4). This effect parallels the increase in expression observed after the addition of the -1300 to -178 SSL/307 fragment. The addition of 110 bp of ocs coding region to the SSL/97 7 promoter (construction 9) did not alter significantly t ...
Multifractal analysis of DNA sequences using a novel chaos
... in terms of mutual information and redundancy (statistical dependence) among subsequence symbols within the DNA sequence is provided. The multifractal spectrum is also shown to be more sensitive for detecting dependence structures within the DNA sequence than the averaged contribution given by redun ...
... in terms of mutual information and redundancy (statistical dependence) among subsequence symbols within the DNA sequence is provided. The multifractal spectrum is also shown to be more sensitive for detecting dependence structures within the DNA sequence than the averaged contribution given by redun ...
Identity elements in tRNA-mediated transcription
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
Chromatin: A sticky silence
... forty fold lower than at the telomeres, depending on the relative strengths of the silencer and promoter tested. As silencer-binding factors function primarily by targeting Sir1p, Sir3p and Sir4p, one can bypass the need for a silencer to repress a reporter gene by replacing it with a Gal4p-binding ...
... forty fold lower than at the telomeres, depending on the relative strengths of the silencer and promoter tested. As silencer-binding factors function primarily by targeting Sir1p, Sir3p and Sir4p, one can bypass the need for a silencer to repress a reporter gene by replacing it with a Gal4p-binding ...
Chapter 5
... The first-generation purple-flowering plants are called hybrid plants. This means they came from true-breeding parent plants with different forms of the same trait. Mendel wondered what would happen if he cross-pollinated two purple-flowering hybrid plants. As shown in Figure 4, some of the offsprin ...
... The first-generation purple-flowering plants are called hybrid plants. This means they came from true-breeding parent plants with different forms of the same trait. Mendel wondered what would happen if he cross-pollinated two purple-flowering hybrid plants. As shown in Figure 4, some of the offsprin ...
Cloning and characterization of a gene coding for a hydrophobin Fv
... terms of molecular biology or molecular genetics (De Groot et al., 1996). In a commercial-scale production of mushrooms, the development of fruiting bodies is induced by controlling various environmental factors such as light, temperature, humidity, physical stimulus, and chemical stimulus. These fa ...
... terms of molecular biology or molecular genetics (De Groot et al., 1996). In a commercial-scale production of mushrooms, the development of fruiting bodies is induced by controlling various environmental factors such as light, temperature, humidity, physical stimulus, and chemical stimulus. These fa ...
Tiger beetles - Discover the Microbes Within!
... The collection was essentially random as I sampled from multiple sites and did not discriminate between which beetles I collected. Collection was done on 2 occasions, off 2 separate trails. Both trails we in the terra Firma but near the flood forests The Samples were stored in 95% alcohol and in a f ...
... The collection was essentially random as I sampled from multiple sites and did not discriminate between which beetles I collected. Collection was done on 2 occasions, off 2 separate trails. Both trails we in the terra Firma but near the flood forests The Samples were stored in 95% alcohol and in a f ...
Neoplasia Lec4
... carcinoma, and basal cell carcinoma It is capable to damage DNA With extensive exposure to sunlight, the repair system is overwhelmed skin cancer They cause mutations in TP53 gene ...
... carcinoma, and basal cell carcinoma It is capable to damage DNA With extensive exposure to sunlight, the repair system is overwhelmed skin cancer They cause mutations in TP53 gene ...
"Vectors in Gene Therapy". In: An Introduction to Molecular Medicine
... The PP tract therefore serves as the primer for synthesis of the second DNA strand. The packaging signal binds to the nucleocapsid protein of a retroviral particle allowing the genomic RNA to be selectively packaged. Although the encapsidation sequence was initially mapped to the region of the virus ...
... The PP tract therefore serves as the primer for synthesis of the second DNA strand. The packaging signal binds to the nucleocapsid protein of a retroviral particle allowing the genomic RNA to be selectively packaged. Although the encapsidation sequence was initially mapped to the region of the virus ...
WNT targets - Stanford University
... Several puzzling observations made previously suggested that target genes that are activated by WNT signaling during development were actively repressed in the absence of the signal. Recent work sheds light on how this switch between repression and activation is regulated. ells within multi-cellular ...
... Several puzzling observations made previously suggested that target genes that are activated by WNT signaling during development were actively repressed in the absence of the signal. Recent work sheds light on how this switch between repression and activation is regulated. ells within multi-cellular ...
223/AP08 - EDVOTEK
... Scientists also identified particular amino acid substitutions in GFP that altered the behavior of its ‘chromophore’, a special structure within the protein that is responsible for light production (Figure 4). Different changes bring about different patterns of light absorption and emission, allowin ...
... Scientists also identified particular amino acid substitutions in GFP that altered the behavior of its ‘chromophore’, a special structure within the protein that is responsible for light production (Figure 4). Different changes bring about different patterns of light absorption and emission, allowin ...
Agrobacterium-mediated DNA transfer, and then some
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
Reduced levels of two modifiers of epigenetic
... towards reduced body weight, a larger standard deviation from the mean and a significantly increased coefficient of variance compared to wild-type littermates (Figure 1). This effect appears to be more marked following paternal inheritance of the mutant allele but this could be the result of the lar ...
... towards reduced body weight, a larger standard deviation from the mean and a significantly increased coefficient of variance compared to wild-type littermates (Figure 1). This effect appears to be more marked following paternal inheritance of the mutant allele but this could be the result of the lar ...
Chapter 18
... Some of the viral proteins made were sent through the endomembrane system to the cell membrane. ...
... Some of the viral proteins made were sent through the endomembrane system to the cell membrane. ...
Chapter 18
... 1. The proteins that encapsulate the genetic material of a virus is known as the _____________. 2. Draw a general structure of a eukaryotic virus and label parts. 3. An individual protein of the structure mentioned in question number 1 is known as a _______________. 4. A bacteriophage can reproduce ...
... 1. The proteins that encapsulate the genetic material of a virus is known as the _____________. 2. Draw a general structure of a eukaryotic virus and label parts. 3. An individual protein of the structure mentioned in question number 1 is known as a _______________. 4. A bacteriophage can reproduce ...
Diapositiva 1
... A non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa The collection includes sequences from plasmids, organelles, viruses, archaea, bacteria, and eukaryotes Each RefSeq represents a single, naturally occurring molecule from one organism. RefSeq biological ...
... A non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa The collection includes sequences from plasmids, organelles, viruses, archaea, bacteria, and eukaryotes Each RefSeq represents a single, naturally occurring molecule from one organism. RefSeq biological ...
Isolation and characterization of an RNA that binds with high affinity
... 4B). By contrast, after addition of a similar amount of tRNA (total tRNA from yeast) the basal level of transcription remained either unaffected or was only marginally reduced (about 10-20%; see F i g . 4). These results demonstrate that factors that are important in the transcription process can bi ...
... 4B). By contrast, after addition of a similar amount of tRNA (total tRNA from yeast) the basal level of transcription remained either unaffected or was only marginally reduced (about 10-20%; see F i g . 4). These results demonstrate that factors that are important in the transcription process can bi ...
Gene Expression
... 8.2 Transcription: From DNA to RNA 8.3 Translation: From mRNA to Protein 8.4 Differences in Gene Expression Between Prokaryotes and Eukaryotes 8.5 A Comprehensive Example: Computerized Analysis of Gene Expression in C. elegans 8.6 The Effect of Mutations on Gene Expression and Gene Function Copyrigh ...
... 8.2 Transcription: From DNA to RNA 8.3 Translation: From mRNA to Protein 8.4 Differences in Gene Expression Between Prokaryotes and Eukaryotes 8.5 A Comprehensive Example: Computerized Analysis of Gene Expression in C. elegans 8.6 The Effect of Mutations on Gene Expression and Gene Function Copyrigh ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.