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Slides
Slides

... Section 18.1: Genetic Information: Replication DNA Recombination – principle source of genetic variations that make evolution possible § Rearrangement of DNA sequences by exchanging segments from different molecules §Two types of recombination: §General recombination occurs between homologous DNA m ...
The Molecular Basis of Inheritance
The Molecular Basis of Inheritance

... A human cell can copy its 6 billion base pairs and divide into daughter cells in only a few hours. ...
CHAPTER 16 THE MOLECULE BASIS OF INHERITANCE
CHAPTER 16 THE MOLECULE BASIS OF INHERITANCE

... A human cell can copy its 6 billion base pairs and divide into daughter cells in only a few hours. ...
The glpP and glpF genes of the glycerol regulon in
The glpP and glpF genes of the glycerol regulon in

... The Bacillus subtilis glpPFKD region contains genes essential for growth on glycerol or glycerol 3-phosphate (G3P). The nucleotide sequence of glpP encoding a regulatory protein and the previously unidentified glpF encoding the glycerol uptake facilitator was determined. glpF is located immediately ...
GOBASE—a database of organelle and bacterial
GOBASE—a database of organelle and bacterial

... sophisticated representation of trans-spliced genes than has previously been possible. Information from the Gene Ontology project (9) has also been integrated into the GOBASE database. Every gene and gene product defined in GOBASE is associated with a suitable set of Gene Ontology terms as determine ...
Genome structure, analysis and evolufion Lecture 1
Genome structure, analysis and evolufion Lecture 1

... 2.  DNA  in  a  chromosome  can  be  transmi\ed  efficiently  to  both   daughter  cells  during  cell  division   3.  Chromosome   confers   an   overall   organiza?on   to   each   molecule  of  DNA,  which  facilitates  gene  expression  as ...
6.1 Mutation
6.1 Mutation

...  In snowy areas, this would increase fitness because these bears would be able to hide easier. They could catch more prey, live longer, and reproduce more. When would this decrease fitness and why?  In darker areas (such as the forest), this would decrease fitness because these bears would stand o ...
Derived copy of Cell Division
Derived copy of Cell Division

... are involved in the organization and packing of the chromosomal DNA into a highly condensed structure. The condensing complex compacts chromosomes, and the resulting condensed structure is necessary for chromosomal segregation during mitosis. ...
LBSC 708L Session 1
LBSC 708L Session 1

... mutant transiently accumulated nitrite in the growth medium, but it had a final growth yield similar to that of the wild type. Transcription of the nirIX gene cluster itself was controlled by NNR, a member of the family of FNR-like transcriptional activators. An NNR binding sequence is located in th ...
PPT4 - Ycmou
PPT4 - Ycmou

...  Genes located along the DNA are transcribed into RNA that, in the case of mRNA, is then translated into protein at the ribosomes.  In other words, DNA determines what proteins and enzymes an organism can synthesize and, therefore, what chemical reactions it is able to carry out. © 2007, YCMOU. Al ...
mutations
mutations

... proven target for broad-spectrum antibacterial therapy and for antituberculosis therapy RNAP is a suitable target for three reasons: (1) RNAP is an essential enzyme (permits efficacy) (2) bacterial RNAP-subunit sequences are highly conserved (permits broad-spectrum activity) (3) bacterial RNAP seque ...
Notes Packet - Ms. Ottolini`s Biology Wiki!
Notes Packet - Ms. Ottolini`s Biology Wiki!

CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA
CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA

... B. Restriction enzyme mapping – Frequently it is important to have a restriction enzyme site map of a cloned gene for further manipulations of the gene. This is accomplished by digestion of the gene singly with several enzymes and then in combinations. The fragments are subjected to gel electrophore ...
Stickler Syndrome
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... collagen, called the pro-alpha1(XI) chain. Type XI collagen adds structure and strength to the connective tissues that support the body's muscles, joints, organs, and skin. Type XI collagen is normally found in cartilage. Type XI collagen is also part of the clear gel that fills the eyeball (the vit ...
Bacteriophage l and Its Relatives
Bacteriophage l and Its Relatives

... Fig. 3. The physical map of the l genome is shown in the upper part of the figure, divided into halves to fit on the page. The scale bar represents the DNA, and the boxes above it show the positions and sizes of the genes. Shaded boxes represent genes transcribed leftward and open boxes genes transc ...
Blueprint of Life by Ahmad Shah Idil
Blueprint of Life by Ahmad Shah Idil

... This suggests that these vertebrates evolved from a common aquatic ancestor, such as the crossopterygian fish. ...
introns within ribosomal protein genes regulate the production and
introns within ribosomal protein genes regulate the production and

... Introns  of  Rp  genes  modulate  the   processing  of  pre-­‐rRNA   •  Processing  intermediated  were    detected  using   qPCR.   •  About  88%  of  all  intron  deleOons  increased  or   decreasd  at  least  one  pre-­‐rRna  processing ...
Recombinant DNA and Gene Cloning
Recombinant DNA and Gene Cloning

... cell ruptured to release its DNA. The tangle is a portion of a single DNA molecule containing over Plasmids are replicated by the 4.6 million base pairs encoding same machinery that replicates the approximately 4,300 genes. The bacterial chromosome. Some small circlets are plasmids. plasmids are cop ...
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... – Vectors (pUC19) carry a segment of regulatory sequences & coding information for first 146 amino acids of the lacZ gene (β-galasidase) ...
Word file: Analysis of alternative splicing in Drosophila
Word file: Analysis of alternative splicing in Drosophila

... comparison of cells growing side by side within the same tissue of the same individual. In addition, because such clones typically encompass only a small fraction of the cells in the organism, they seldom impair survival. Thus even splicing factors with essential roles in viability can be studied. A ...
Bacterial Handout #3 Genetics 200A September 24, 2012 Genetic
Bacterial Handout #3 Genetics 200A September 24, 2012 Genetic

... carbon source. High glucose levels lead to inactivation of adenylate cyclase (Cya), lowering cAMP concentrations. Likewise, under starvation conditions or growth on unfavorable carbon sources leads to activation of Cya and increased cAMP levels. The concentration of cAMP is read out by the catabo ...
University of Debrecen - DEA
University of Debrecen - DEA

... polysaccharide. The lipid and polysaccharide are linked in the outer membrane to form a complex (lipopolysaccharide - LPS). The polysaccharide portion of LPS consists of two components, the core polysaccharide and the O-polysaccharide. The outer half of the outer membrane bilayer is mainly, while li ...
Chapter 21
Chapter 21

... sites (within a genome) or individuals • Simple sequence DNA is common in centromeres and telomeres, where it probably plays structural roles in the chromosome ...
Evolution of prokaryotic genomes
Evolution of prokaryotic genomes

... DNA rearrangements and (4) acquisition of genetic information. Each of these categories again includes a variety of specific mechanisms. In addition, some of the observed processes may overlap with more than one of the listed categories. For example, intrinsic limits of structural stability of nucle ...
SICB 2014 Annual Meeting Abstracts
SICB 2014 Annual Meeting Abstracts

... gene regulatory regions through a highly conserved DNA binding domain (DBD) composed of three C−terminal C2H2 zinc fingers (KLF−DBD). Members of the Klf gene family influence transcription via interactions with other transcription factors, cofactors, chromatin remodeling factors, and transcriptional ...
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Primary transcript



A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.
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