rpoB gene sequence-based characterization of emerging non
... Over the past 10 years, 16S rRNA gene sequencing has contributed to the establishment of more than 45 novel species of non-tuberculous mycobacteria and to the description of emerging mycobacterial infections. Cumulative experience has indicated that this molecular tool underestimates the diversity o ...
... Over the past 10 years, 16S rRNA gene sequencing has contributed to the establishment of more than 45 novel species of non-tuberculous mycobacteria and to the description of emerging mycobacterial infections. Cumulative experience has indicated that this molecular tool underestimates the diversity o ...
Properties and sequence of the coenzyme B12
... was ampli¢ed by PCR and cloned into pBluescript SK+ in the same orientation as the T7 promoter. The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the t ...
... was ampli¢ed by PCR and cloned into pBluescript SK+ in the same orientation as the T7 promoter. The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the t ...
A natural chimeric yeast containing genetic material from three species
... al., 1985). The ATP9 genes in the isolates analysed were also found to be 228 bp long (Fig. 2). The amino acid sequences were identical in all cases, but several silent substitutions were observed. The A TP9 gene sequences of Saccharomyces sp. CID 1 and Saccharomyces sp. I F 0 1802 were identical. A ...
... al., 1985). The ATP9 genes in the isolates analysed were also found to be 228 bp long (Fig. 2). The amino acid sequences were identical in all cases, but several silent substitutions were observed. The A TP9 gene sequences of Saccharomyces sp. CID 1 and Saccharomyces sp. I F 0 1802 were identical. A ...
as Word 2000
... recommend the “Social Gene” and “Population Explosion” as a good pair to begin with. In each Round, during Phase 3, “New Genes” you should turn over two new genes, immediately after the leading player has chosen which genes they want to buy. The second placed player is therefore the first to have th ...
... recommend the “Social Gene” and “Population Explosion” as a good pair to begin with. In each Round, during Phase 3, “New Genes” you should turn over two new genes, immediately after the leading player has chosen which genes they want to buy. The second placed player is therefore the first to have th ...
Document
... Codon (3mer) -based models are not nearly as information rich as dicodon-based models Tricodon (9mers)-based models need too many data points for it to be practical People have used 7-mer or 8-mer based models; they could provide better prediction methods 6-mer based models There are 4*4*4 = 64 codo ...
... Codon (3mer) -based models are not nearly as information rich as dicodon-based models Tricodon (9mers)-based models need too many data points for it to be practical People have used 7-mer or 8-mer based models; they could provide better prediction methods 6-mer based models There are 4*4*4 = 64 codo ...
Origin and evolution of Y chromosomes: Drosophila tales
... The D. melanogaster Y chromosome comprises mainly repetitive DNA and is heterochromatic. It does not determine sex; instead, sex determination in Drosophila is basically accomplished by a count of the number of X chromosomes [14,15]. However, males devoid of Y chromosomes (‘X0 males’) are sterile [1 ...
... The D. melanogaster Y chromosome comprises mainly repetitive DNA and is heterochromatic. It does not determine sex; instead, sex determination in Drosophila is basically accomplished by a count of the number of X chromosomes [14,15]. However, males devoid of Y chromosomes (‘X0 males’) are sterile [1 ...
The β-Globin LCR is Not Necessary for an Open Chromatin
... Hispanic thalassemia removes ~35 kb of DNA upstream resulting in failure to activate the β-globin locus at the level of transcription All five HSs form when chromosome 11 is transferred to an erythroid environment 5’HSs increase expression of β-globin genes in transfection and transgenic analyses Al ...
... Hispanic thalassemia removes ~35 kb of DNA upstream resulting in failure to activate the β-globin locus at the level of transcription All five HSs form when chromosome 11 is transferred to an erythroid environment 5’HSs increase expression of β-globin genes in transfection and transgenic analyses Al ...
A whole-genome assembly of the domestic cow, Bos taurus
... Another indicator of assembly completeness, and also of its potential for annotation, is the extent to which known gene sequences can be mapped onto it. We aligned 8,689 independently validated full-length cow mRNA sequences to the two assemblies, using spliced alignment mapping tools (see Materials ...
... Another indicator of assembly completeness, and also of its potential for annotation, is the extent to which known gene sequences can be mapped onto it. We aligned 8,689 independently validated full-length cow mRNA sequences to the two assemblies, using spliced alignment mapping tools (see Materials ...
Phenotypic overlap in the contribution of individual genes to CNV
... organisms and single-gene human hereditary disorders and thus speed the interpretation of the complex phenotypes of CNV disorders. There are currently more than 5000 human genes about which nothing is known phenotypically, but for which detailed phenotypic information for their mouse and/or zebrafis ...
... organisms and single-gene human hereditary disorders and thus speed the interpretation of the complex phenotypes of CNV disorders. There are currently more than 5000 human genes about which nothing is known phenotypically, but for which detailed phenotypic information for their mouse and/or zebrafis ...
Genetic suppression
... inviable or subviable, especially at low temperature (Waterston, 1981). Presumably the deleterious effects result from too much readthrough of natural stop codons. Selection for improved low-temperature viability of sup-7 animals was used to generate deletions or loss-of-function mutations of sup-7, ...
... inviable or subviable, especially at low temperature (Waterston, 1981). Presumably the deleterious effects result from too much readthrough of natural stop codons. Selection for improved low-temperature viability of sup-7 animals was used to generate deletions or loss-of-function mutations of sup-7, ...
Plant–nematode interactions
... plant-parasitic nematode species, have been produced [42]. Existing EST collections from plant-parasitic nematodes are mostly derived from eggs and infective juveniles, but future projects will likely expand to include the parasitic stages [27,34,42]. DNA-sequence analyses of these ESTs have shown ...
... plant-parasitic nematode species, have been produced [42]. Existing EST collections from plant-parasitic nematodes are mostly derived from eggs and infective juveniles, but future projects will likely expand to include the parasitic stages [27,34,42]. DNA-sequence analyses of these ESTs have shown ...
pdf
... removal of up to 60 Tg of nitrogen annually, roughly an eighth of the global fixed nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentiall ...
... removal of up to 60 Tg of nitrogen annually, roughly an eighth of the global fixed nitrogen sink. Although denitrification has long been believed to be the major process responsible for fixed nitrogen loss from the oceans, recent studies show that anaerobic ammonium oxidation (anammox) is potentiall ...
SOP Criteria for Variant Classification
... Avoid applying similar evidence twice: e.g. PS2 and PM6 (de novo criteria) cannot be both invoked for a variant in a patient; if all evidences from the literature were considered and counted and related criteria are invoked, then PP5 or BP6 cannot be invoked again as an additional line of evidence ...
... Avoid applying similar evidence twice: e.g. PS2 and PM6 (de novo criteria) cannot be both invoked for a variant in a patient; if all evidences from the literature were considered and counted and related criteria are invoked, then PP5 or BP6 cannot be invoked again as an additional line of evidence ...
Prothrombin Gene Mutation - Hamilton Health Sciences
... It causes the body to make too much prothrombin, which makes the blood more likely to clot. Blood clots in your lungs or the deep veins of your legs can be dangerous. ...
... It causes the body to make too much prothrombin, which makes the blood more likely to clot. Blood clots in your lungs or the deep veins of your legs can be dangerous. ...
Ch08_complete-Inheritance,_Genes
... 1. In the genetic cross AaBbCcDdEE x AaBBCcDdEe where all the genes are unlinked, what fractions of offspring will be heterozygous for all of these genes? 2. In a plant species, two alleles control flower color, which can be yellow, blue, or white. Crosses of these plants produce the offspring provi ...
... 1. In the genetic cross AaBbCcDdEE x AaBBCcDdEe where all the genes are unlinked, what fractions of offspring will be heterozygous for all of these genes? 2. In a plant species, two alleles control flower color, which can be yellow, blue, or white. Crosses of these plants produce the offspring provi ...
Supplementary Notes S1 (doc 64K)
... following settings: log2 ratio >0.2 (duplications) or <0.2 (deletions) with a minimum of 5 probes affected, maximum probe distance was <10 Kb (>10Kn these were treated as separate CNVs). All CNVs identified each hybridization were visually assessed for confidence and to determine if it was present i ...
... following settings: log2 ratio >0.2 (duplications) or <0.2 (deletions) with a minimum of 5 probes affected, maximum probe distance was <10 Kb (>10Kn these were treated as separate CNVs). All CNVs identified each hybridization were visually assessed for confidence and to determine if it was present i ...
Genome-Wide Gene Expression Effects of Sex Chromosome
... parent-of-origin of their X and Y chromosomes. We used compound X-X and X-Y chromosomes to generate three contrasts (Figure 1): males that inherit a maternal X chromosome and a paternal Y chromosome (XMYP) vs. males that inherit a paternal X and a maternal Y (XPYM); males that inherit a maternal com ...
... parent-of-origin of their X and Y chromosomes. We used compound X-X and X-Y chromosomes to generate three contrasts (Figure 1): males that inherit a maternal X chromosome and a paternal Y chromosome (XMYP) vs. males that inherit a paternal X and a maternal Y (XPYM); males that inherit a maternal com ...
Supplemental figure 1 Complete CLSM stacks of Ad3 texas
... the Eco RV-linearised form of the three plasmids ppolyAd∆EP-TETP, ppoly-Ad∆EPTETP-∆24, ppoly-Ad∆EP-TETP-∆24∆19 and a modified form of the 28 kb Cla I- Pac I fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearise ...
... the Eco RV-linearised form of the three plasmids ppolyAd∆EP-TETP, ppoly-Ad∆EPTETP-∆24, ppoly-Ad∆EP-TETP-∆24∆19 and a modified form of the 28 kb Cla I- Pac I fragment containing a Swa I restriction site inserted into the deleted fiber region 7. The second recombination was between the Swa I-linearise ...
The tightly regulated promoter of the xanA gene of
... genes of the purine degradation pathway, necessitating the specific UaY transcription factor and the AreA GATA factor. The promoter of the duplicated region is active ectopically and the difficulty in detecting an mRNA from the duplicated region is at least partially due to nonsense-mediated decay. ...
... genes of the purine degradation pathway, necessitating the specific UaY transcription factor and the AreA GATA factor. The promoter of the duplicated region is active ectopically and the difficulty in detecting an mRNA from the duplicated region is at least partially due to nonsense-mediated decay. ...
Article The Landscape of Realized Homologous
... replacement of DNA in the recipient genome with homologous sequence from a donor. However, the intensity of this gene-conversion-like process varies markedly between species (Perez-Losada et al. 2006; Vos 2009), ranging from clonal species (Smith et al. 2006) to those that exchange 10% or more of th ...
... replacement of DNA in the recipient genome with homologous sequence from a donor. However, the intensity of this gene-conversion-like process varies markedly between species (Perez-Losada et al. 2006; Vos 2009), ranging from clonal species (Smith et al. 2006) to those that exchange 10% or more of th ...
File
... (c) In all animals so far studied, the production of fully functional sperm is sensitive to temperature. In the nematode worm, Caenorhabditis elegans, spermatogenesis takes place in a similar way to mammals. Two proteins known as argonaute proteins are important in the development of sperm. They are ...
... (c) In all animals so far studied, the production of fully functional sperm is sensitive to temperature. In the nematode worm, Caenorhabditis elegans, spermatogenesis takes place in a similar way to mammals. Two proteins known as argonaute proteins are important in the development of sperm. They are ...
R4, a non-LTR retrotransposon specific to the
... the free-living nematode Caenorhabditis elegans. It was unlikely that R4 elements were present in C.elegans, as variant rDNA units had been previously characterized from this species and no insertion elements were found (26). Only the PCR amplifications with P.equorum and H.contortus DNA gave rise t ...
... the free-living nematode Caenorhabditis elegans. It was unlikely that R4 elements were present in C.elegans, as variant rDNA units had been previously characterized from this species and no insertion elements were found (26). Only the PCR amplifications with P.equorum and H.contortus DNA gave rise t ...
Chap3_110718_textbook
... How do we decide whether a chi-square statistic is likely too large to be due to sampling effects alone? To do this, we compare the chi-square value for our experiment to a previously calculated probability distribution for all possible chi-square values. This distribution shows the probability of o ...
... How do we decide whether a chi-square statistic is likely too large to be due to sampling effects alone? To do this, we compare the chi-square value for our experiment to a previously calculated probability distribution for all possible chi-square values. This distribution shows the probability of o ...
DFL1, an auxin-responsive GH3 gene homologue, negatively
... had fully opened cotyledons whereas the wild type still had closed cotyledons (data not shown). We could not observe any differences in cotyledon opening between d¯1-D and wild type at the ¯uence rates used for red and far-red light. These results indicate that d¯1-D is more sensitive than the wild ...
... had fully opened cotyledons whereas the wild type still had closed cotyledons (data not shown). We could not observe any differences in cotyledon opening between d¯1-D and wild type at the ¯uence rates used for red and far-red light. These results indicate that d¯1-D is more sensitive than the wild ...
31 Shareable Fragile X Facts (National Fragile X Foundation)
... Fragile X Awareness month is here! We’ve put together a list of 31 facts that you can share each day of the week on sites like Facebook and Twitter. If you share them, please use the hashtags #FragileXFacts [2] and #KnowFragileX [3]! 1. Fragile X encompasses a group of conditions that can affect fam ...
... Fragile X Awareness month is here! We’ve put together a list of 31 facts that you can share each day of the week on sites like Facebook and Twitter. If you share them, please use the hashtags #FragileXFacts [2] and #KnowFragileX [3]! 1. Fragile X encompasses a group of conditions that can affect fam ...