A set reduction and pattern matching problem motivated by Allele
... the conserved regions (dark blue areas) of a family specific consensus sequence and used to amplify members of the gene family. 2) Analysis of the amplified family members identifies regions specific to two subsets of the family (shown in red). These regions allows the amplified regions to be divide ...
... the conserved regions (dark blue areas) of a family specific consensus sequence and used to amplify members of the gene family. 2) Analysis of the amplified family members identifies regions specific to two subsets of the family (shown in red). These regions allows the amplified regions to be divide ...
Identification of Potential Corynebacterium ammoniagenes Purine
... strains used for the production of IMP and GMP are primarily obtained by either chemical or spontaneous mutagenesis. However, these processes result in cumulative genetic alterations that limit the generation of improved bacterial strains for the production of purine nucleotides. Therefore, developm ...
... strains used for the production of IMP and GMP are primarily obtained by either chemical or spontaneous mutagenesis. However, these processes result in cumulative genetic alterations that limit the generation of improved bacterial strains for the production of purine nucleotides. Therefore, developm ...
Updated slides on gene prediction
... • Allows for higher precision in identifying true ORFs; much better than merely testing for length. • However, average vertebrate exon length is 130 nucleotides, which is often too small to produce reliable peaks in the likelihood ratio • Further improvement: in-frame hexamer count (frequencies of p ...
... • Allows for higher precision in identifying true ORFs; much better than merely testing for length. • However, average vertebrate exon length is 130 nucleotides, which is often too small to produce reliable peaks in the likelihood ratio • Further improvement: in-frame hexamer count (frequencies of p ...
The Drosophila Gene Disruption Project: Progress
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
... ABSTRACT The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These strains often disrupt gene function directly, allow production of new alleles, and have many other applications for a ...
Analysis of DNA transcription termination sequences of gene coding
... palindromic sequence, which is constituted of 24 bp with high GC content (Figure 2). The detailed analysis have indicated that this dyad symmetry is a transcription terminator. The regulation of synthases phaC1 and phaC2 gene expression was examined in Pseudomonas corrugata (Conte et al. 2006). In t ...
... palindromic sequence, which is constituted of 24 bp with high GC content (Figure 2). The detailed analysis have indicated that this dyad symmetry is a transcription terminator. The regulation of synthases phaC1 and phaC2 gene expression was examined in Pseudomonas corrugata (Conte et al. 2006). In t ...
Regulatory Genes Controlling MPG7 Expression
... a minority of fungi are devastating pathogens of healthy eukaryotic hosts and can exploit the host environment by a variety of specific adaptations. The rice blast fungus Magnaporthe grisea provides a striking example of adaptations for pathogenicity and can be easily manipulated for both genetic an ...
... a minority of fungi are devastating pathogens of healthy eukaryotic hosts and can exploit the host environment by a variety of specific adaptations. The rice blast fungus Magnaporthe grisea provides a striking example of adaptations for pathogenicity and can be easily manipulated for both genetic an ...
Splice Site Prediction Using Artificial Neural Networks
... The task is to predict splice sites, thus the desired output is 1.0 when a splice site is in the middle of the sliding window. There are two outputs from the neural network: One for indicating acceptor splice site and one for indicating donor splice site. However, if it is only a 1.0 output when a s ...
... The task is to predict splice sites, thus the desired output is 1.0 when a splice site is in the middle of the sliding window. There are two outputs from the neural network: One for indicating acceptor splice site and one for indicating donor splice site. However, if it is only a 1.0 output when a s ...
Direct Sequence Analysis of the 14q+ and 18q
... equivalents. This could be due to either polymorphic variation or somatic mutation, which is known to occur during D-J recombination. In contrast, the bcl-2 sequence showed no evidence of mutation. In every junction there was an intervening sequence between bcl-2 and J,, although in patient D this c ...
... equivalents. This could be due to either polymorphic variation or somatic mutation, which is known to occur during D-J recombination. In contrast, the bcl-2 sequence showed no evidence of mutation. In every junction there was an intervening sequence between bcl-2 and J,, although in patient D this c ...
A Comparative Genomic Analysis of Two Distant Diptera, the Fruit
... In a first set of experiments aiming at exploring long-range synteny and microsynteny, we identified, among the currently available A. gambiae sequences, putative orthologs of genes in which in D. melanogaster are clustered within two well-studied chromosomal regions, each nearly 3 Mb long. We then ...
... In a first set of experiments aiming at exploring long-range synteny and microsynteny, we identified, among the currently available A. gambiae sequences, putative orthologs of genes in which in D. melanogaster are clustered within two well-studied chromosomal regions, each nearly 3 Mb long. We then ...
Simplified Insertion of Transgenes Onto Balancer Chromosomes via
... ABSTRACT Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchan ...
... ABSTRACT Balancer chromosomes are critical tools for Drosophila genetics. Many useful transgenes are inserted onto balancers using a random and inefficient process. Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchan ...
Molecular Genetics and Genomics
... mutants fail to form infection threads. EPSII is a galactoglucan with a completely dierent structure; EPSIIde®cient mutants are still able to form an eective symbiosis with alfalfa. Twenty-two exo genes for EPSI synthesis have been identi®ed in R. meliloti Rm2011; 19 of them reside in a contiguous ...
... mutants fail to form infection threads. EPSII is a galactoglucan with a completely dierent structure; EPSIIde®cient mutants are still able to form an eective symbiosis with alfalfa. Twenty-two exo genes for EPSI synthesis have been identi®ed in R. meliloti Rm2011; 19 of them reside in a contiguous ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... fork movement (its sense is located on the lagging strand) (French 1992) because of head-on collisions between DNA and RNA polymerases (Brewer 1988). Therefore, transcription of lowly expressed genes would not interfere with replication and interruption of their transcription should not be as delete ...
... fork movement (its sense is located on the lagging strand) (French 1992) because of head-on collisions between DNA and RNA polymerases (Brewer 1988). Therefore, transcription of lowly expressed genes would not interfere with replication and interruption of their transcription should not be as delete ...
Genomic surveys and expression analysis of bZIP gene family in
... bZIP members evolved in monocots than in dicots after the divergence of monocots from dicots (Wang et al. 2011). DNA‑binding‑site specificity and classification of RcbZIP proteins The basic and hinge regions in the bZIP domains directly interacting with DNA cis-elements are the most conserved region ...
... bZIP members evolved in monocots than in dicots after the divergence of monocots from dicots (Wang et al. 2011). DNA‑binding‑site specificity and classification of RcbZIP proteins The basic and hinge regions in the bZIP domains directly interacting with DNA cis-elements are the most conserved region ...
Chapter 1.
... gene. The series of derived lines each with a single major resistance gene are known as near-isogenic lines. The NILs are similar for all traits except the major resistance genes. DNA polymorphisms between different NILs are likely to be associated with the different resistance genes. Screening mole ...
... gene. The series of derived lines each with a single major resistance gene are known as near-isogenic lines. The NILs are similar for all traits except the major resistance genes. DNA polymorphisms between different NILs are likely to be associated with the different resistance genes. Screening mole ...
Divergent Amphibian Species Nonclassical MHC Class I Lineages
... an amino acid alignment of SNC and XNC a1 domains was made. This alignment was used to generate a neighbor-joining phylogenetic tree. Orthologous XNC and SNC gene subfamilies were defined as any clade having a bootstrap value of 75 or higher. As such, the amino acid sequence similarity of individual ...
... an amino acid alignment of SNC and XNC a1 domains was made. This alignment was used to generate a neighbor-joining phylogenetic tree. Orthologous XNC and SNC gene subfamilies were defined as any clade having a bootstrap value of 75 or higher. As such, the amino acid sequence similarity of individual ...
Detection of the Most Common Genetic Causes of
... 1A Y isoform gene (EIF1AY) and the RNA binding motif (RBM) family are found on AZFb region. EIF1AY encodes an essential translation factor. The PTP-BL-related Y (PRY) family of genes is mapped to AZFb and AZFc regions and encodes proteins proposed to be involved in apoptosis. RBM and deleted-in-azoo ...
... 1A Y isoform gene (EIF1AY) and the RNA binding motif (RBM) family are found on AZFb region. EIF1AY encodes an essential translation factor. The PTP-BL-related Y (PRY) family of genes is mapped to AZFb and AZFc regions and encodes proteins proposed to be involved in apoptosis. RBM and deleted-in-azoo ...
SNPs for individual identification
... that will be put into a database analogous to CODIS, these additional criteria include: a. No medical or sensitive personal information is conveyed by the individual or combined data. Ideally the SNP is not in a “gene” (but what is a gene? See panel). b. “Highly informative” is interpreted as high h ...
... that will be put into a database analogous to CODIS, these additional criteria include: a. No medical or sensitive personal information is conveyed by the individual or combined data. Ideally the SNP is not in a “gene” (but what is a gene? See panel). b. “Highly informative” is interpreted as high h ...
Research Project Final Report
... for human consumption. The two species are closely related, but have different breeding systems, pea being inbred and faba bean open pollinated. Thus the genetics of the two is very different. The pea genome is large, about the same size as the barley genome, while faba bean, although a diploid, is ...
... for human consumption. The two species are closely related, but have different breeding systems, pea being inbred and faba bean open pollinated. Thus the genetics of the two is very different. The pea genome is large, about the same size as the barley genome, while faba bean, although a diploid, is ...
Chapter 6
... The dorsoventral axis inversion theory holds that protostomes and deuterostomes have an inverted dorsoventral organization (Arendt and Nübler-Jung 1994). It was originally based on anatomical observations (Geoffroy St. Hilaire 1822) and has recently received support from molecular data (De Robertis ...
... The dorsoventral axis inversion theory holds that protostomes and deuterostomes have an inverted dorsoventral organization (Arendt and Nübler-Jung 1994). It was originally based on anatomical observations (Geoffroy St. Hilaire 1822) and has recently received support from molecular data (De Robertis ...
Conspiracy of silence among repeated transgenes
... conjecture might be correct. When vertebrates are transformed with exogenous DNA, chromosomal insertions almost always consist of long tandem arrays of the transfected transgene-containing DNA.(5) Sometimes transgene arrays can comprise hundreds or even thousands of copies. In a cytologic study of o ...
... conjecture might be correct. When vertebrates are transformed with exogenous DNA, chromosomal insertions almost always consist of long tandem arrays of the transfected transgene-containing DNA.(5) Sometimes transgene arrays can comprise hundreds or even thousands of copies. In a cytologic study of o ...
Journal of Bacteriology
... was then introduced into pGem7zf(⫹) (Invitrogen Inc.) with EcoRI and BamHI cleavage sites. The resulting plasmid was digested with the NarI restriction enzyme and was then ligated with the PCR product containing the kanamycin resistance gene that was amplified from pKD4 (9) by using the Kd4kanF and ...
... was then introduced into pGem7zf(⫹) (Invitrogen Inc.) with EcoRI and BamHI cleavage sites. The resulting plasmid was digested with the NarI restriction enzyme and was then ligated with the PCR product containing the kanamycin resistance gene that was amplified from pKD4 (9) by using the Kd4kanF and ...
Reduced penetrance in human inherited disease
... In diseases which exhibit locus heterogeneity clinical penetrance may vary between mutations in different genes as in deafness [24]. Reduced penetrance may also be characteristic of many triplet repeat expansion disorders as in Huntington disease. The possession of intragenic (HTT) CAG repeats of 36 ...
... In diseases which exhibit locus heterogeneity clinical penetrance may vary between mutations in different genes as in deafness [24]. Reduced penetrance may also be characteristic of many triplet repeat expansion disorders as in Huntington disease. The possession of intragenic (HTT) CAG repeats of 36 ...
Integrated genome sequence and linkage map of physic nut
... and 0.746 Mbp, respectively, which are approximately 1.8 and 46.8 times longer, respectively, than the reported physic nut genome (Hirakawa et al., 2012). As the result of short contigs and scaffolds, too many predicted genes in their study were fragmented. Only 17 Mbp (6%) of their assembly (but ab ...
... and 0.746 Mbp, respectively, which are approximately 1.8 and 46.8 times longer, respectively, than the reported physic nut genome (Hirakawa et al., 2012). As the result of short contigs and scaffolds, too many predicted genes in their study were fragmented. Only 17 Mbp (6%) of their assembly (but ab ...
Differential chromatin packaging of genomic
... and mouse loci including the c-Ha-Ras-1 gene (30–32). Variation in the methylation of allelic sites is tissue specific and reproducible after transmission through the germ line. A putative cis-acting element(s) must be close to the Zfp127 locus to explain the complete cosegregation observed in seven ...
... and mouse loci including the c-Ha-Ras-1 gene (30–32). Variation in the methylation of allelic sites is tissue specific and reproducible after transmission through the germ line. A putative cis-acting element(s) must be close to the Zfp127 locus to explain the complete cosegregation observed in seven ...