A Major Species of Mouse μ-opioid Receptor mRNA and Its
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
... 3ⴕ-RACE and DNA Sequencing. Total RNA was extracted from mouse brain as described under Northern Blot Analysis. The 3⬘RACE experiments were performed as described in the protocol of 3⬘-RACE System for rapid amplification of cDNA ends Kit (Invitrogen, Carlsbad, CA). In brief, the first-strand cDNA wa ...
Genetics of Hemophilia
... hemophilia. This allows family members to make informed decisions about their care, and obtain the proper treatment and information. »» If you are a carrier, but do not have a known family history of hemophilia, it is still possible that there are other women in your family who carry the gene for he ...
... hemophilia. This allows family members to make informed decisions about their care, and obtain the proper treatment and information. »» If you are a carrier, but do not have a known family history of hemophilia, it is still possible that there are other women in your family who carry the gene for he ...
Controversial cases of human gender identification by amelogenin test
... Gender identification is essential on forensic science to provide the initial information for criminal investigation. However, gender identification by amplification of amelogenin gene sometimes showed controversial cases, in which male samples was falsely identified as females. We reported the anom ...
... Gender identification is essential on forensic science to provide the initial information for criminal investigation. However, gender identification by amplification of amelogenin gene sometimes showed controversial cases, in which male samples was falsely identified as females. We reported the anom ...
Artificial selection on introduced Asian haplotypes shaped the
... in order to improve traits of commercial interest. Current genomics techniques enabled genome-wide identification of these Asian introgressed haplotypes in modern European pig breeds. We propose that the Asian variants are still present because they affect phenotypes that were important for ancient ...
... in order to improve traits of commercial interest. Current genomics techniques enabled genome-wide identification of these Asian introgressed haplotypes in modern European pig breeds. We propose that the Asian variants are still present because they affect phenotypes that were important for ancient ...
Correlation of ABCA4 polymorphisms with age
... take part in the functions of photoreceptor outer segments as a flipase of all-trans retinal. Allikmets and his colleagues proved that the distemperedness of this gene can lead to macular disorders [11, 12]. Other research found that they are the same ABC transporter through comparing ABCA4 with the ...
... take part in the functions of photoreceptor outer segments as a flipase of all-trans retinal. Allikmets and his colleagues proved that the distemperedness of this gene can lead to macular disorders [11, 12]. Other research found that they are the same ABC transporter through comparing ABCA4 with the ...
CHAPTER 7
... Plugging these values into our chi square formula, along with the observed numbers, we get: ...
... Plugging these values into our chi square formula, along with the observed numbers, we get: ...
Reference genome sequence of the model plant Setaria
... Small RNAs (smRNAs) were analyzed by comparing ~10.3 million smRNA reads from inflorescence and leaf (http://smallrna.udel.edu/ project_data.php) of Setaria inbred Yugu1 with known microRNAs (miRNAs) (Supplementary Table 5) and with the annotated trans posable element component of the Setaria genom ...
... Small RNAs (smRNAs) were analyzed by comparing ~10.3 million smRNA reads from inflorescence and leaf (http://smallrna.udel.edu/ project_data.php) of Setaria inbred Yugu1 with known microRNAs (miRNAs) (Supplementary Table 5) and with the annotated trans posable element component of the Setaria genom ...
Genetics of allergic disease
... specific IgE levels to allergens as a representative for atopy have been presented so far. In the European Community Respiratory Health Survey of 13,963 asthma patients, the complex segregation analysis of the asthma phenotype provides evidence for a two-allele gene with co-dominant inheritance11. O ...
... specific IgE levels to allergens as a representative for atopy have been presented so far. In the European Community Respiratory Health Survey of 13,963 asthma patients, the complex segregation analysis of the asthma phenotype provides evidence for a two-allele gene with co-dominant inheritance11. O ...
Marwa Yahia Ahmed_o
... alone. In vitro experiments have revealed that an excess of 10-100 times the amount of IL-1Ra is necessary to inhibit IL-1 activity whereas, in vivo, studies showed that 1002000 times more IL-1Ra is needed (Pelletier et al., 1995). ...
... alone. In vitro experiments have revealed that an excess of 10-100 times the amount of IL-1Ra is necessary to inhibit IL-1 activity whereas, in vivo, studies showed that 1002000 times more IL-1Ra is needed (Pelletier et al., 1995). ...
What is a genome?
... It's a fair bet that if it's fair tomorrow, then my fair wife and I will head to the Spring Fair, held in a fair sized park, in this fair city to win a prize, if everyone plays fair We will have a number of sequences that will read 'fair' and we would not know how to assemble them ...
... It's a fair bet that if it's fair tomorrow, then my fair wife and I will head to the Spring Fair, held in a fair sized park, in this fair city to win a prize, if everyone plays fair We will have a number of sequences that will read 'fair' and we would not know how to assemble them ...
Sense and Nonsense in the Genetic Code
... of the rII region are shown to be transcribedas separatemessengerRNA moleculeswhich are translatedinto separateproteins.In the deletionmutant,a single messengerRNA molecule is formed which is tlanslatedinto a singleproteincapableof performingthe B functionbut not the A function.In the deletionmutant ...
... of the rII region are shown to be transcribedas separatemessengerRNA moleculeswhich are translatedinto separateproteins.In the deletionmutant,a single messengerRNA molecule is formed which is tlanslatedinto a singleproteincapableof performingthe B functionbut not the A function.In the deletionmutant ...
MOLECULAR PROFILING OF RICE (Oryza sativa L
... plants to limit the penetration, development and or reproduction of invading pathogens. Tolerance of host plants is measured in terms of the ability to maintain growth and yield production in spite of infection or invasion of pathogens. Although both factors are genetically controlled, the environme ...
... plants to limit the penetration, development and or reproduction of invading pathogens. Tolerance of host plants is measured in terms of the ability to maintain growth and yield production in spite of infection or invasion of pathogens. Although both factors are genetically controlled, the environme ...
Application of the new manP counter-selection system for B. subtilis
... et al., 2003; Westers et al., 2004) or to introduce new metabolic routes, requires ...
... et al., 2003; Westers et al., 2004) or to introduce new metabolic routes, requires ...
pdf
... reaction volume. A touchdown PCR program, similar to that used by Braker et al. (5), was used for the amplification of nirK with the Cunir3 and Cunir4 (Cunir3-4) primers. Denaturation at 95°C for 2 min was followed by 10 cycles of 94°C for 30 s, 45°C (⫺0.5°C per cycle) for 40 s, and 72°C for 40 s, 2 ...
... reaction volume. A touchdown PCR program, similar to that used by Braker et al. (5), was used for the amplification of nirK with the Cunir3 and Cunir4 (Cunir3-4) primers. Denaturation at 95°C for 2 min was followed by 10 cycles of 94°C for 30 s, 45°C (⫺0.5°C per cycle) for 40 s, and 72°C for 40 s, 2 ...
BMC Genomics 10
... mapped in humans. In addition, comparison to human genes is a tool to link characteristics to causative genes in future QTL studies. Especially for insufficiently described regions, the comparative mapping provides the possibility of identifying central genes through programmes like GeneDistiller [1 ...
... mapped in humans. In addition, comparison to human genes is a tool to link characteristics to causative genes in future QTL studies. Especially for insufficiently described regions, the comparative mapping provides the possibility of identifying central genes through programmes like GeneDistiller [1 ...
Potato chromosomes IX and XI carry genes for resistance to potato
... scorable PCR marker linked to Gm (Fig. 2, lane 6). Mapping of the PVM resistance gene Rm The screening of the parental DNA of the ‘‘Rm’’ population with 56 ISSR and 185 RAPD primers resulted in detection of 121 (23%) and 485 (32%) polymorphic DNA fragments. However, only one 1,079 bp ISSR product, a ...
... scorable PCR marker linked to Gm (Fig. 2, lane 6). Mapping of the PVM resistance gene Rm The screening of the parental DNA of the ‘‘Rm’’ population with 56 ISSR and 185 RAPD primers resulted in detection of 121 (23%) and 485 (32%) polymorphic DNA fragments. However, only one 1,079 bp ISSR product, a ...
08_chapter 1
... showed that ATS and GCS in the entire regio~ are almost zero. 1.2.1.1. Studies using regression analysis on sample DNA fragments A generalized presentation on the Chargaffs 2nd parity was given by Prabhu (1993). He studied all the available sequences in the GenBank which are 50000 base pair or more. ...
... showed that ATS and GCS in the entire regio~ are almost zero. 1.2.1.1. Studies using regression analysis on sample DNA fragments A generalized presentation on the Chargaffs 2nd parity was given by Prabhu (1993). He studied all the available sequences in the GenBank which are 50000 base pair or more. ...
Des - Evolution of Developmental Genes
... • Cloning of opossum IGF2 was succesful • 3 of 4 sequences were identical to each other and to opossum IGF-2 ...
... • Cloning of opossum IGF2 was succesful • 3 of 4 sequences were identical to each other and to opossum IGF-2 ...
Off-target Effects: Disturbing the Silence of RNA
... Early studies in RNAi established that single base pair mismatches between the siRNA and the target transcript dramatically alter siRNA functionality.13 From this result it was inferred that overall sequence identity played a role in siRNA specificity, thus leading to the adoption of local alignment ...
... Early studies in RNAi established that single base pair mismatches between the siRNA and the target transcript dramatically alter siRNA functionality.13 From this result it was inferred that overall sequence identity played a role in siRNA specificity, thus leading to the adoption of local alignment ...
Noise in eukaryotic gene expression
... noise in GFPmut3b expression can, when assuming similar mRNA decay rates, be used as an indicator of tetR gene expression noise. By using a value of 4 AU for tetR gene expression noise in the simulations, we overestimate repressor fluctuations and show that, in the absence of transcriptional reiniti ...
... noise in GFPmut3b expression can, when assuming similar mRNA decay rates, be used as an indicator of tetR gene expression noise. By using a value of 4 AU for tetR gene expression noise in the simulations, we overestimate repressor fluctuations and show that, in the absence of transcriptional reiniti ...
publication
... Informatics database map and the Davis Human/Mouse homology map have been made by identifying human orthologs to mapped mouse genes (8). Detailed characterizations of disease regions are increasingly being done through mouse–human genomic comparisons aimed at identifying novel genes and regulatory e ...
... Informatics database map and the Davis Human/Mouse homology map have been made by identifying human orthologs to mapped mouse genes (8). Detailed characterizations of disease regions are increasingly being done through mouse–human genomic comparisons aimed at identifying novel genes and regulatory e ...
Technical Summary - Office of the Gene Technology Regulator
... Events that do not lead to an adverse outcome, or could not reasonably occur, do not represent an identified risk and do not advance any further in the risk assessment process. Nine events were identified whereby the proposed dealings might give rise to harm to people or the environment. This includ ...
... Events that do not lead to an adverse outcome, or could not reasonably occur, do not represent an identified risk and do not advance any further in the risk assessment process. Nine events were identified whereby the proposed dealings might give rise to harm to people or the environment. This includ ...
Siamese Breeding Policy - Siamese Cat Joint Advisory Committee
... chromosomes, each made up of thousands of genes. Each gene has a complicated chemical structure which acts as a template for the construction of components of the body. The exceptional cells are the egg and the sperm, which have only one set of chromosomes - formed by random selection of one from ea ...
... chromosomes, each made up of thousands of genes. Each gene has a complicated chemical structure which acts as a template for the construction of components of the body. The exceptional cells are the egg and the sperm, which have only one set of chromosomes - formed by random selection of one from ea ...
A Study of Gene Linkage and Mapping Using Tetrad Analysis
... could use the same approach to develop detailed predictions for the linked model (with genes on opposite or on the same side of the centromere). All models presented here consider only single crossovers. The effect of multiple crossovers and the use of mapping functions and the Poisson distribution ...
... could use the same approach to develop detailed predictions for the linked model (with genes on opposite or on the same side of the centromere). All models presented here consider only single crossovers. The effect of multiple crossovers and the use of mapping functions and the Poisson distribution ...