... ion? Please show your calculations. Choice B: The standard free energy for the transfer of a 20 residue Gly peptide (Gly20) into a phospholipid bilayer is +60 kJ/M. The standard free energy for the transfer of the sidechain of Cysteine to a nonpolar environment is –3 kJ/mol. You add large amounts of ...
Protein-Protein Interaction Networks
... databases Dissemination is a condition of funding (in US) Makes biology different from most other disciplines! ...
... databases Dissemination is a condition of funding (in US) Makes biology different from most other disciplines! ...
Shah - Buffalo Ontology Site
... that is never really defined anywhere • Genomics data is considered high throughput if: • You can not “look” at your data to interpret it • Generally speaking it means ~ 1000 or more genes and 20 or more samples. ...
... that is never really defined anywhere • Genomics data is considered high throughput if: • You can not “look” at your data to interpret it • Generally speaking it means ~ 1000 or more genes and 20 or more samples. ...
Bacterial enzymes that can deglycate glucose
... ‘gfr’, to indicate enzymes that degrade ‘glucation’ and ‘fructation’ products. In order to clarify the biochemical function of this novel protein (GfrE), and that of the FrlB homologue GfrF (Figure 2B), Wiame et al. [14] PCR-amplified gfrF and gfrE from the genomic DNA of E. faecium, subcloned the p ...
... ‘gfr’, to indicate enzymes that degrade ‘glucation’ and ‘fructation’ products. In order to clarify the biochemical function of this novel protein (GfrE), and that of the FrlB homologue GfrF (Figure 2B), Wiame et al. [14] PCR-amplified gfrF and gfrE from the genomic DNA of E. faecium, subcloned the p ...
Inborn Errors of Metabolism
... • Most prevalent disorder caused by inborn errors of amino acid metabolism • Caused by mutations in phenyalanine hydroxylase (PAH) gene •PAH converts phenyalanine into tyrosine and requires the cofactor tetrahydrobiopterin (BH4), molecular oxygen and iron •Loss of PAH activity increased concentrat ...
... • Most prevalent disorder caused by inborn errors of amino acid metabolism • Caused by mutations in phenyalanine hydroxylase (PAH) gene •PAH converts phenyalanine into tyrosine and requires the cofactor tetrahydrobiopterin (BH4), molecular oxygen and iron •Loss of PAH activity increased concentrat ...
Functomics!?
... or mutation of regions involved in activity or binding (to metals, nucleotides, etc), presence of paralogs, contradiction with the biological context (i.e. if a protein belongs to a pathway supposed to be absent in a particular organism), etc. Such "problematic" proteins will not be automatically an ...
... or mutation of regions involved in activity or binding (to metals, nucleotides, etc), presence of paralogs, contradiction with the biological context (i.e. if a protein belongs to a pathway supposed to be absent in a particular organism), etc. Such "problematic" proteins will not be automatically an ...
Biochemistry 2000 Sample Questions 4 RNA, Lipids, Membranes 1
... (d) The energetically least favourable reaction in glycolysis is catalyzed by ________________. (8) Glucose-6-phosphate isomerase catalyzes the interconversion of glucose-6phosphate (an aldose) and fructose-6-phosphate (a ketose). Draw the structure(s) of the reaction intermediate(s). (9) Which glyc ...
... (d) The energetically least favourable reaction in glycolysis is catalyzed by ________________. (8) Glucose-6-phosphate isomerase catalyzes the interconversion of glucose-6phosphate (an aldose) and fructose-6-phosphate (a ketose). Draw the structure(s) of the reaction intermediate(s). (9) Which glyc ...
Bchm2000_P5 - U of L Class Index
... (d) The energetically least favourable reaction in glycolysis is catalyzed by ________________. (8) Glucose-6-phosphate isomerase catalyzes the interconversion of glucose-6phosphate (an aldose) and fructose-6-phosphate (a ketose). Draw the structure(s) of the reaction intermediate(s). (9) Which glyc ...
... (d) The energetically least favourable reaction in glycolysis is catalyzed by ________________. (8) Glucose-6-phosphate isomerase catalyzes the interconversion of glucose-6phosphate (an aldose) and fructose-6-phosphate (a ketose). Draw the structure(s) of the reaction intermediate(s). (9) Which glyc ...
Whole-cell biocatalysts by design - Microbial Cell Factories
... the synthesis of rhamnolipids from the cheap raw material butane using a tailored whole-cell biocatalyst. The AlkBGT system from Pseudomonas putida (for butane activation) and the RhlABC system from Pseudomonas aeruginosa (for rhamnolipid assembly) were integrated into the cell strain to result in r ...
... the synthesis of rhamnolipids from the cheap raw material butane using a tailored whole-cell biocatalyst. The AlkBGT system from Pseudomonas putida (for butane activation) and the RhlABC system from Pseudomonas aeruginosa (for rhamnolipid assembly) were integrated into the cell strain to result in r ...
Chapter 9. Cellular Respiration Other Metabolites
... Respond to cell’s needs Key point of control ...
... Respond to cell’s needs Key point of control ...
Energy, enzymes and metabolism
... Enzymes can also change the reaction pathway by holding the reactants in a specific configuration, or by altering the charge distribution or shape of the substrate. ...
... Enzymes can also change the reaction pathway by holding the reactants in a specific configuration, or by altering the charge distribution or shape of the substrate. ...
FG-NEMs
... • Perturbation of genes followed by high-throughput profiling of different phenotypes can be used to characterize functions of genes • However, most genes do not function independently but interact in a network to drive a particular function • Phenotypic measurements (e.g. mRNA levels) are indirect ...
... • Perturbation of genes followed by high-throughput profiling of different phenotypes can be used to characterize functions of genes • However, most genes do not function independently but interact in a network to drive a particular function • Phenotypic measurements (e.g. mRNA levels) are indirect ...
Acid Base Balance
... PH (CONT’D) Acids are formed as end products of protein, carbohydrate, and fat metabolism To maintain the body’s normal pH (7.35-7.45), the H+ must be neutralized or excreted The bones, lungs, and kidneys are the major organs involved in the regulation of acid and base balance ...
... PH (CONT’D) Acids are formed as end products of protein, carbohydrate, and fat metabolism To maintain the body’s normal pH (7.35-7.45), the H+ must be neutralized or excreted The bones, lungs, and kidneys are the major organs involved in the regulation of acid and base balance ...
Acid Base Balance
... PH (CONT’D) Acids are formed as end products of protein, carbohydrate, and fat metabolism To maintain the body’s normal pH (7.35-7.45), the H+ must be neutralized or excreted The bones, lungs, and kidneys are the major organs involved in the regulation of acid and base balance ...
... PH (CONT’D) Acids are formed as end products of protein, carbohydrate, and fat metabolism To maintain the body’s normal pH (7.35-7.45), the H+ must be neutralized or excreted The bones, lungs, and kidneys are the major organs involved in the regulation of acid and base balance ...
Professor Jason Raymond - School of Earth and Space Exploration
... The key role of oxygen (O2) for (complex) life on our planet • Complex life as we know it is impossible without an ...
... The key role of oxygen (O2) for (complex) life on our planet • Complex life as we know it is impossible without an ...
sample written evaluation
... pathway to produce amino acids. In general amino acids resulting from diverting metabolites early in the metabolic pathway entail higher cost. The cost calculations for different growth substrates were highly correlated > 0.9. Average costs were used for subsequent analysis of correlation between co ...
... pathway to produce amino acids. In general amino acids resulting from diverting metabolites early in the metabolic pathway entail higher cost. The cost calculations for different growth substrates were highly correlated > 0.9. Average costs were used for subsequent analysis of correlation between co ...
Compare and Contrast table for Photosynthesis and Cellular
... 6.) Inorganic molecules that bind to enzymes and are necessary for their functioning. ____________ 7.) The type of pathway body builders are interested in maximizing. ________________ 8.) The free energy of a non-spontaneous reaction. ____________________ 9.) Energy can neither be created nor destro ...
... 6.) Inorganic molecules that bind to enzymes and are necessary for their functioning. ____________ 7.) The type of pathway body builders are interested in maximizing. ________________ 8.) The free energy of a non-spontaneous reaction. ____________________ 9.) Energy can neither be created nor destro ...
Compare and Contrast table for Photosynthesis and Cellular
... 6.) Inorganic molecules that bind to enzymes and are necessary for their functioning. ____________ 7.) The type of pathway body builders are interested in maximizing. ________________ 8.) The free energy of a non-spontaneous reaction. ____________________ 9.) Energy can neither be created nor destro ...
... 6.) Inorganic molecules that bind to enzymes and are necessary for their functioning. ____________ 7.) The type of pathway body builders are interested in maximizing. ________________ 8.) The free energy of a non-spontaneous reaction. ____________________ 9.) Energy can neither be created nor destro ...
Minimal metabolic pathway structure is consistent with
... networks of Escherichia coli (Orth et al, 2011) and Saccharomyces cerevisiae (Mo et al, 2009). They contain 750 and 332 pathways, respectively, representing the dimensions of the two null spaces (see Supplementary Dataset S1). For each calculated MinSpan pathway, we grouped the “gene-protein-reactio ...
... networks of Escherichia coli (Orth et al, 2011) and Saccharomyces cerevisiae (Mo et al, 2009). They contain 750 and 332 pathways, respectively, representing the dimensions of the two null spaces (see Supplementary Dataset S1). For each calculated MinSpan pathway, we grouped the “gene-protein-reactio ...
erma application internal cover sheet
... DNA will be sequenced, and proteins will be expressed from recombinant DNA. E. coli or P. pastoralis containing genes from photosynthetic eukarya will be used to compare enzyme functions in primary metabolic pathways, e.g., reductive sulphur assimilation, ethylene biosynthesis, where differences occ ...
... DNA will be sequenced, and proteins will be expressed from recombinant DNA. E. coli or P. pastoralis containing genes from photosynthetic eukarya will be used to compare enzyme functions in primary metabolic pathways, e.g., reductive sulphur assimilation, ethylene biosynthesis, where differences occ ...
Local anesthesia Systemic Complications
... Weakest anesthetic in the minimum volume (compatible with successful anesthesia) Inject slowly (minimum of 60 sec / 1.8 ml) Continually observe Never leave patient alone after injection ...
... Weakest anesthetic in the minimum volume (compatible with successful anesthesia) Inject slowly (minimum of 60 sec / 1.8 ml) Continually observe Never leave patient alone after injection ...
Metabolic network modelling
Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑