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Energy coupling in Saccharomyces cerevisiae
Energy coupling in Saccharomyces cerevisiae

Reactome Functional Interaction (FI) Network
Reactome Functional Interaction (FI) Network

... 3. GO annotation and pathway analysis (e.g. Reactome): what biological terms or functional categories are represented in the gene list more often than expected by chance; 4. Network-based analysis. - identify markers not as individual genes but as sub-networks extracted from protein interaction data ...
Identification of Unknown Bacterial Species - Katie Davis
Identification of Unknown Bacterial Species - Katie Davis

... that the organism is MR positive. If Vogues-Proskauer reagents are added and a red color is formed, that indicates that the organism is VP positive (3). My organism was VP positive and MR negative because the media turned red only when Vogues Proskauer reagent was added. The nitrate broth contains ...
Sodexo 2008 - MUSC Health
Sodexo 2008 - MUSC Health

... management of prematures infants. Start parenteral nutrition (PN) soon after birth, with amino acids within two hours and lipids within 24 hours of birth, in infants < 1.5 kg. Promote and use human milk as the preferred nutritional substrate for premature infants Implement feeding guidelines which i ...
Partial Class Notes Chapter 6-8 ENZYME#2
Partial Class Notes Chapter 6-8 ENZYME#2

Partial Class Notes Chapter 6-8 ENZYME#2
Partial Class Notes Chapter 6-8 ENZYME#2

... • increased interaction of E with S in transition-state (ES‡) • E distorts S, forcing it toward the transition state • E must be complementary to transition-state in shape and chemical character • E binds transition states 1010 to 1015 times more tightly than S ...
Methanol - ACuteTox
Methanol - ACuteTox

... Formic acid inhibits cytochrome oxidase activity by binding to the ferric iron moiety; it is less potent in this regard than cyanide and carbon monoxide. The acidosis present in severe methanol poisoning increases the concentration of undissociated formic acid, thus potentiating inhibition of cellul ...
BIOCHEMISTRY
BIOCHEMISTRY

... monitoring and screening. Tests used primarily for diagnosis may also provide prognostic information and some are used specifically for this purpose. A major use of biochemical tests is to follow the course of an illness and to monitor the effects of treatment. Biochemical tests can also be used to ...
Biochemistry_Written_Tests.doc
Biochemistry_Written_Tests.doc

Nutrition Support: Enteral And Parenteral Nutrition Implication
Nutrition Support: Enteral And Parenteral Nutrition Implication

understanding carbon metabolism in hydrogen production
understanding carbon metabolism in hydrogen production

THE USE OF TBE ETHANOL PATHWAY IN GOLDFISH CARASSIUS
THE USE OF TBE ETHANOL PATHWAY IN GOLDFISH CARASSIUS

1. The PERL script to
1. The PERL script to

... Searching the Kyoto Encyclopaedia of Genes and Genomes (KEGG) database Introduction The KEGG database was searched for pathway information of the genes on the microarray using the GO database updated gene list. The search retrieved pathway information from different species including a direct link w ...
Peptides, Proteins, and Enzymes
Peptides, Proteins, and Enzymes

... 15.  Understand the terms cofactor and coenzyme. 16.  Understand how enzymes work and distinguish between absolute specificity, relative specificity, and stereospecificity. 17.  Understand and define the terms: essential amino acid, complete protein, incomplete protein, and complimentary protein. 18 ...
A criticism of the value of midparent in
A criticism of the value of midparent in

... which have an integral multiple of the chromosome haploid number, in contrast to aneuploids which possess a chromosome set that is not an exact multiple of the haploid number and which usually underperform the parents and show undesirable traits (Comai, 2005). Anomalous features are also observed in ...
Purine metabolism - mustafaaltinisik.org.uk
Purine metabolism - mustafaaltinisik.org.uk

... Amidophosphoribosyl transferase is a important regulatory enzyme in purine biosynthesis. It is strongly inhibited by the end products IMP, AMP, and GMP. This type of inhibition is called FEEDBACK INHIBITION. ...
Bacterial Unknowns
Bacterial Unknowns

... negative, however your sample has been mixed with a gram positive in order to better resemble a real‐ life situation. The first step to this project will be the isolation of your Gram negative bacteria in order to  complete the rest of your tests. The key to successful biochemical testing is the use ...
PPT File
PPT File

... start a reaction uncatalyzed reaction ...
Citrate Cycle
Citrate Cycle

... This coupled redox reaction directly links the citrate cycle to the electron transport system through the redox conjugate pair FAD/FADH2 which is covalently linked to the enzyme succinate dehydrogenase, an inner mitochondrial membrane protein. Oxidation of succinate results in the transfer of an ele ...
Determination of activity and Michaelis constant of LD in biological
Determination of activity and Michaelis constant of LD in biological

... 4. Try to explain why the increasing order of lactate concentration and the constant NAD + concentration were used for determination of KM for lactate? What would you do if you were to determine KM for NAD+? 5. Why is it good to add EDTA into the buffer used for homogenisation? Conclusion: ...
Microbial diversity and virulence probing of five different body sites
Microbial diversity and virulence probing of five different body sites

... Selected only 2 metagenomes (ear samples) to generate radial tree. Pie charts and tables created by clicking node on Radial Tree. Radial tree customized to display at either Phyla or Class level. ...
bioinformatics review - e
bioinformatics review - e

... Summary: Independent of the platform and the analysis methods used, the result of a microarray experiment is, in most cases, a list of differentially expressed genes. An automatic ontological analysis approach has been recently proposed to help with the biological interpretation of such results. Cur ...
ACID*BASE DISORDERS
ACID*BASE DISORDERS

... Expected [HCO3] – 24 - (2x2) = 20meq/l Actual [HCO3] – 15meq/l Step 4 - any other process involved? Actual pH &[HCO3] values are both LOWER than the expected values. So, which could lower ...
Respiratory Acidosis
Respiratory Acidosis

...  (5) hyperviscosity and severe hyperlipidemia, which can lead to an underestimation of sodium and chloride concentrations.  A fall in serum albumin by 1 g/dL from the normal value ...
mammalian hibernation: biochemical adaptation
mammalian hibernation: biochemical adaptation

... mammals arise from two main effects of cold on metabolic systems that have been optimized over millions of years of mammalian evolution for function within a narrow temperature window. The first is the differential effect of temperature change on the rates of thousands of cellular reactions that can ...
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Metabolic network modelling



Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑
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