The role of xylulokinase in Saccharomyces cerevisiae xylulose
... with D-xylulose, 500 with ATP). Km and Vmax were obtained from Hanes^Woolf plots of the presented data. All these data were obtained under similar conditions at the same pH (pH 6.5). From these data, an equilibrium constant of 90 was calculated using the Haldane relationship Keq = (Vmax forward/Vmax ...
... with D-xylulose, 500 with ATP). Km and Vmax were obtained from Hanes^Woolf plots of the presented data. All these data were obtained under similar conditions at the same pH (pH 6.5). From these data, an equilibrium constant of 90 was calculated using the Haldane relationship Keq = (Vmax forward/Vmax ...
Mechanism of Succinyl
... The conformation for Citrate Synthase changes when oxaloacetate (OAA) binds to citrate synthase. This conformational change creates the acetyl-CoA binding site. Once OAA is bound, the binding constant for acetyl-CoA is increased by a factor of 20. To see OAA, click After OAA is bound to the enzyme, ...
... The conformation for Citrate Synthase changes when oxaloacetate (OAA) binds to citrate synthase. This conformational change creates the acetyl-CoA binding site. Once OAA is bound, the binding constant for acetyl-CoA is increased by a factor of 20. To see OAA, click After OAA is bound to the enzyme, ...
Problem 14. MAGNESIUM DETERMINATION
... and 2, it can also be found in a mixed state with the wavefunction ...
... and 2, it can also be found in a mixed state with the wavefunction ...
3. BIOMOLECULES I. CARBOHYDRATES
... 2.5. 12.2.5. The phenylalanine and tryptophan biosynthesis ......................................... 78 3. 12.3. Protein Synthesis ........................................................................................................ 78 3.1. 12.3.1. The transcription .............................. ...
... 2.5. 12.2.5. The phenylalanine and tryptophan biosynthesis ......................................... 78 3. 12.3. Protein Synthesis ........................................................................................................ 78 3.1. 12.3.1. The transcription .............................. ...
Impact of carbon sources on growth and oxalate - The Keep
... The impact of various supplemental carbon sources (oxalate, glyoxylate, glycolate, pyruvate, formate, malate, acetate, and succinate) on growth and oxalate formation (i.e., oxalogenesis) by Sclerotinia sclerotiorum was ...
... The impact of various supplemental carbon sources (oxalate, glyoxylate, glycolate, pyruvate, formate, malate, acetate, and succinate) on growth and oxalate formation (i.e., oxalogenesis) by Sclerotinia sclerotiorum was ...
Pipecleaner Proteins Lab
... Everything in science is done with models – the scientific method itself is about modeling complex ideas into simpler formats so that we can better understand them. Scientific models may also help us to do things that would otherwise be impossible. For example, there is no way that we could have seq ...
... Everything in science is done with models – the scientific method itself is about modeling complex ideas into simpler formats so that we can better understand them. Scientific models may also help us to do things that would otherwise be impossible. For example, there is no way that we could have seq ...
Chapter 10
... – Stage 4: electron transport, the transfer of electrons from reduced coenzymes to oxygen coupled to active transport of protons across a membrane – Stage 5: The electrochemical proton gradient formed in step 4 is used to drive ATP synthesis ...
... – Stage 4: electron transport, the transfer of electrons from reduced coenzymes to oxygen coupled to active transport of protons across a membrane – Stage 5: The electrochemical proton gradient formed in step 4 is used to drive ATP synthesis ...
Metabolism
... Most of the foods and drinks people ingest are complex materials that the body must break down into simpler substances. This process may involve several steps. The simpler substances are then used as building blocks, which are assembled into the materials the body needs to sustain life. The process ...
... Most of the foods and drinks people ingest are complex materials that the body must break down into simpler substances. This process may involve several steps. The simpler substances are then used as building blocks, which are assembled into the materials the body needs to sustain life. The process ...
A comparison of the amino acid sequence of the
... half its size. This homology is particularly marked in regions adjacent to the catalytic triad Asp32, His64 and Ser221 of subtilisin BPN’. Significant features of the A. salmonicida enzyme, a new member of the group of cysteine-containingsubtilisin-type serine proteases, are the presence of six cyst ...
... half its size. This homology is particularly marked in regions adjacent to the catalytic triad Asp32, His64 and Ser221 of subtilisin BPN’. Significant features of the A. salmonicida enzyme, a new member of the group of cysteine-containingsubtilisin-type serine proteases, are the presence of six cyst ...
IMGT/Collier-de-Perles: a two-dimensional visualization tool for
... The IMGT/Collier-de-Perles [14] tool was created by LIGM (Université Montpellier 2, CNRS) and is part of IMGTW, the international ImMunoGeneTics information systemW [15,16] (IMGTW, http://www.imgt.org), which is acknowledged as the global reference in immunogenetics and immunoinformatics. IMGT/Colli ...
... The IMGT/Collier-de-Perles [14] tool was created by LIGM (Université Montpellier 2, CNRS) and is part of IMGTW, the international ImMunoGeneTics information systemW [15,16] (IMGTW, http://www.imgt.org), which is acknowledged as the global reference in immunogenetics and immunoinformatics. IMGT/Colli ...
The Glutaric Acidurias of the Amish: A Sense of Progress 1988
... During my fellowship years urine organic acids were done with wide-bore, hand-packed column on a Varian GC with a flame ionization detector, not a mass spectrometer. Tanaka’s methylene unit calculations were used to identify compounds. Few of the specimens that were run on the GC were later run thro ...
... During my fellowship years urine organic acids were done with wide-bore, hand-packed column on a Varian GC with a flame ionization detector, not a mass spectrometer. Tanaka’s methylene unit calculations were used to identify compounds. Few of the specimens that were run on the GC were later run thro ...
The rotary mechanism of the ATP synthase Archives - iGRAD
... Because of the large size, multiple subunits many of which are integral membrane proteins, and asymmetry, determination of the subunit stoichiometry and defining subunit interactions has been challenging. There is still some debate about the number of c subunits and whether this number can vary withi ...
... Because of the large size, multiple subunits many of which are integral membrane proteins, and asymmetry, determination of the subunit stoichiometry and defining subunit interactions has been challenging. There is still some debate about the number of c subunits and whether this number can vary withi ...
bcaa power - ProAction
... succinyl-CoA, a Krebs cycle intermediate; isoleucine generates one molecule of succinyl-CoA and one of acetyl-CoA; and the complete catabolism of leucine produces three molecules of acetyl-CoA, and this process continues in the Krebs cycle until oxidation is complete. The principal point where BCAA ...
... succinyl-CoA, a Krebs cycle intermediate; isoleucine generates one molecule of succinyl-CoA and one of acetyl-CoA; and the complete catabolism of leucine produces three molecules of acetyl-CoA, and this process continues in the Krebs cycle until oxidation is complete. The principal point where BCAA ...
Solving Biochemistry`s Biggest Mystery: How We Produce Energy
... Part 1: The discovery of coenzyme Q-10. An Interview with Dr. Fred L Crane by Richard A. Passwater, Ph.D. More than half of the people in the United States take a daily vitamin supplement. Most of these individuals don’t even realize that this was not possible not too awfully long ago. Thanks to a s ...
... Part 1: The discovery of coenzyme Q-10. An Interview with Dr. Fred L Crane by Richard A. Passwater, Ph.D. More than half of the people in the United States take a daily vitamin supplement. Most of these individuals don’t even realize that this was not possible not too awfully long ago. Thanks to a s ...
Biochemists Break the Code
... Binding of a new aa-tRNA at the A site -EF-Tu (GTP), EF-Ts Formation of the new peptide bond (Transpeptidation)-23S rRNA Translocation of the Ribosome -EF-G(GTP) Repeat and Repeat until the stop codon enters the A site Binding of a new aa-tRNA at the A site At the start of each cycle, the A site on ...
... Binding of a new aa-tRNA at the A site -EF-Tu (GTP), EF-Ts Formation of the new peptide bond (Transpeptidation)-23S rRNA Translocation of the Ribosome -EF-G(GTP) Repeat and Repeat until the stop codon enters the A site Binding of a new aa-tRNA at the A site At the start of each cycle, the A site on ...
AP Biology - Richfield Public Schools
... Draw an electron shell diagram for each atom, label the valence electrons and identify how many bonds this atom can make. Using the structural formula draw a molecule of these atoms bonded together to complete their valence electrons. (Hint: you may have to use more than one of each atom) ...
... Draw an electron shell diagram for each atom, label the valence electrons and identify how many bonds this atom can make. Using the structural formula draw a molecule of these atoms bonded together to complete their valence electrons. (Hint: you may have to use more than one of each atom) ...
Systembiologische Ansätze zur Erforschung des Metabolismus
... • Not all 2k binary flux patterns need be tested by using constraint that each new flux pattern cannot be written as a combination of previously found elementary flux patterns. • Is fixed-parameter tractability problem ...
... • Not all 2k binary flux patterns need be tested by using constraint that each new flux pattern cannot be written as a combination of previously found elementary flux patterns. • Is fixed-parameter tractability problem ...
Thin-Layer Chromatography: Applying TLC as a
... However, salicylic acid and acetylsalicylic acid were only separated slightly from one another, having an Rf difference of 0.096, much lower than the theoretical separation of the standards (Rf: 0.654) (Table 2). One possible explanation for this occurrence may be due to internal physical interacti ...
... However, salicylic acid and acetylsalicylic acid were only separated slightly from one another, having an Rf difference of 0.096, much lower than the theoretical separation of the standards (Rf: 0.654) (Table 2). One possible explanation for this occurrence may be due to internal physical interacti ...
Lecture 3: Introduction to Proteins
... Draw the structure of a typical amino acid, indicating the following features: α-carbon, α-carboxyl group, α-amino group, side chain (“R group”), and ionic forms that predominate at acidic (say, pH 1), neutral (pH 7), and basic (pH 13) pH values. Classify each of the 20 common amino acids found in p ...
... Draw the structure of a typical amino acid, indicating the following features: α-carbon, α-carboxyl group, α-amino group, side chain (“R group”), and ionic forms that predominate at acidic (say, pH 1), neutral (pH 7), and basic (pH 13) pH values. Classify each of the 20 common amino acids found in p ...
Vitamins
... Dehydrogenase complex: 1. The keto C of pyruvate reacts with the carbanion of TPP on E1 to yield an addition compound. The electron-pulling (+) charged N of the thiazole ring promotes CO2 loss. Hydroxyethyl-TPP ...
... Dehydrogenase complex: 1. The keto C of pyruvate reacts with the carbanion of TPP on E1 to yield an addition compound. The electron-pulling (+) charged N of the thiazole ring promotes CO2 loss. Hydroxyethyl-TPP ...
lecture notes
... by means other than hydrolysis and oxidation, often forming a new double bond or a new ring structure e.g. CH3COCO-OH → CH3COCHO (dehydratase) - Isomerases: catalyze the interconversion of isomers. e.g.phosphoglucose isomerase that converts glucose-6phosphate to fructose-6-phosphate. - Ligases: cata ...
... by means other than hydrolysis and oxidation, often forming a new double bond or a new ring structure e.g. CH3COCO-OH → CH3COCHO (dehydratase) - Isomerases: catalyze the interconversion of isomers. e.g.phosphoglucose isomerase that converts glucose-6phosphate to fructose-6-phosphate. - Ligases: cata ...
The therapeutic implications of ketone bodies
... efficiency than does the metabolism of ketones are of two types: the architecture of the pathway of fatty acid oxidation and the enzymatic changes induced by elevation of free fatty acids. During b oxidation, only half of the reducing equivalents released enter the respiratory chain at NADH dehydroge ...
... efficiency than does the metabolism of ketones are of two types: the architecture of the pathway of fatty acid oxidation and the enzymatic changes induced by elevation of free fatty acids. During b oxidation, only half of the reducing equivalents released enter the respiratory chain at NADH dehydroge ...
Citric acid cycle
The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.