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Amino Acid Analysis Quick Reference Card For Hydrolysate
Amino Acid Analysis Quick Reference Card For Hydrolysate

... acid internal standard by reconstituting one vial of Hydrolysates Standards - 114 Labeled with Sample Diluent Amino Acid. The amount of Sample Diluent - Amino Acid to use is indicated on the Certificate of Analysis (approximately 1.67 mL). 2. Vortex to mix, then spin. The iTRAQ™ Reagent 114-labeled ...
Basic Biochemistry
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...  Together with Phosphorus these are the fundamental elements of life  Figure 2-5, page 27 (2-5, page 27)  This figure represents an -amino acid  Consists of C => The Central carbon atom NH2 => Amino group COOH => Carboxyl (acid) group ...
Role of Krebs Cycle in the Mechanism of Stability Internal Medium
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... thermodynamic system of a human organism and the open non equilibrium nonlinear thermodynamic system of an organism’s cells display balance catabolic exergonic processes and anabolic endergonic processes [1,2]. Catabolic anaerobic oxidative phosphorylation of glycolysis exerts the driving mechanism ...
Textbook of Biochemistry - OSU Biochemistry and Molecular Biology
Textbook of Biochemistry - OSU Biochemistry and Molecular Biology

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ХРОМАТОГРАММЫ
ХРОМАТОГРАММЫ

... The results of such researches enable to distinguish "normal" metabolism from a “modified“ one and since the year 2002 has being successfully applied in USA, Britain, Japan and EU countries in the development of new medicines and methods of molecular diagnostics. They accelerate the development of ...
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biochemistry - Textbooks Online
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... liver is most likely the result of degradation of hepatic proteins. Amino acids from these are rapidly used as substrate for gluconeogenesis. Alanine from skeletal muscles is also a major contributor to hepatic glucose production. However, degradation of muscle proteins begins somewhat later than he ...
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... demand for ATP is low, the high ATP/ ADP ratio is used to produce creatine-phosphate, which can then regenerate ATP from ADP when the ATP/ADP ratio falls (Bessman and Geiger, 1981). This latter system is important in muscle to support contractile function during periods of prolonged ATP demand. Aden ...
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Mutations Lab
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... such as phosphofructokinase, phosphoglucoisomerase, glyceraldehyde-3-P-dehydrogenase, phosphoglycerate phosphokinase and pyruvate kinase; though showed higher activity in cytosolic fraction, were present in both cytoplasmic and leucoplasitc fractions. These results are consistent with the proposal t ...
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... In the last chapter we learned about the amino acid composition and the structure of proteins. We also studied the process by which proteins are synthesized from information coded in the genes of the chromosomes. Of the body proteins, perhaps the most important group are the enzymes — the subject of ...
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SUPERCRITICAL CO EXTRACTION OF HEMP (Cannabis sativa L
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... Prepared fatty acid methyl esters (EN ISO 5509:2000 standard) were analyzed by gas chromatography according to EN ISO 5508:1995. It was used 7890B gas chromatograph (Agilent Technologies, Lake Forest, USA) with a capillary column, HP88 100 m long with a diameter of 0.25 mm and the thickness of the s ...
The Glucose/Fatty Acid Cycle 1963–2003
The Glucose/Fatty Acid Cycle 1963–2003

Structure-Guided Site-Directed Mutagenesis of the Bacterial ATP
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... Phenotypic effects of the mutations were observed after transforming each pMBε01 mutant into the XH1 expression strain (Xiong and Vik 1995). The XH1 expression strain has a deletion of the chromosomal epsilon gene but expresses all other subunits of the enzyme. This phenotypic assay measures growth ...
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Citric acid cycle



The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.
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