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2282 MC-025 Bax 2D2 for pdf
2282 MC-025 Bax 2D2 for pdf

... mitochondria during apoptosis, and overexpression of Bax can accelerate cell death. Coregulation of Bax dimer formation and intracellular localization are associated with Bax conformational changes. Anti-Bax YTH-2D2 is specific for human Bax and does not cross react with mouse or rat Bax. When used ...
Gene7-08
Gene7-08

... on free ribosomes. Some have signals for targeting to organelles such as the nucleus or mitochondria. Proteins that are localized cotranslationally associate with the ER membrane during synthesis, so their ribosomes are "membranebound". The proteins pass into the endoplasmic reticulum, along to the ...
Diffusion through a Membrane
Diffusion through a Membrane

... Molecules are constantly moving. Diffusion occurs when the molecules of a substance move from high concentrations, where there are more molecules, to low concentrations, where there are fewer molecules. Diffusion occurs because collisions between moving molecules cause them to move further apart. Di ...
Cell Membrane and Sub Cellular Components
Cell Membrane and Sub Cellular Components

... membranes, in the irregular spaces between the hydrophobic tails of the membrane lipids, where it confers a stiffening and strengthening effect on the membrane. Phospholipids forming lipid vesicles Lipid vesicles or liposomes are circular pockets that are enclosed by a lipid bilayer. These structure ...
Ch 9 modified
Ch 9 modified

... is key to the sorting mechanism. – This sorting mechanism is very different from the sorting mechanisms used in the Golgi apparatus. ...
Simple Diffusion
Simple Diffusion

... Nonionized = Lipid soluble drug can cross cell membranes Ionized = Water soluble ...
Cell Cycle-Dependent Targeting of a Kinesin at the Plasma
Cell Cycle-Dependent Targeting of a Kinesin at the Plasma

... Confocal microscopy of GFP-tagged KCA1 in BY-2 cells was consistent with accumulation at the cell plate and the plasma membrane (Figure 1A and Figure S2). The fluorescence intensity at the plasma membrane was strongest in dividing cells and showed a sharp increase in cells that entered mitosis (Figu ...
Concentration Dependent Different Action of Tamoxifen on
Concentration Dependent Different Action of Tamoxifen on

... TAM and derivatives have been shown to protect biological membranes, and membrane-model systems such as liposomes, against free radical mediated lipid peroxidation. Observation of this protective action of TAM and its derivatives can be attributed to molecular modulation of the membrane environment ...
Modes of action of disinfectants
Modes of action of disinfectants

... Iodine interferes at the level of the respiratory chain of the microorganisms by blocking the t r a n s p o r t of electrons through electrophilic reactions with the enzymes of the respiratory chain. Iodine also interacts preferentially with the proteins of the cytoplasm membrane in a form with a po ...
Diffusion and Membranes
Diffusion and Membranes

... attached to the lipids and proteins of the outer membrane. These are known as glycolipids and glycoproteins. Project out into watery fluids surrounding the cell where they form hydrogen bonds with water. They help stabilise the membrane structure. Can act as receptor molecules for hormones or neurot ...
biological membranes and membrane transport
biological membranes and membrane transport

... polarized. For example, the intracellular face has negative potential of ~60 mV. 8. Membrane potentials play a key role in transport, energy conversion, and ...
Receptors
Receptors

... gene transcription ...
Biochemical Aspects of Lipid Storage and
Biochemical Aspects of Lipid Storage and

... Noble et al. (1969) have shown a good correlation between human serum lipoprotein analyses by analytical ultracentrifugation and agarose gel electrophoresis, thus establishing the latter method as an inexpensive alternative to the former. Detection limits for the electrophoretic visualization of lip ...
A Caveolin Dominant Negative Mutant Associates with Lipid Bodies
A Caveolin Dominant Negative Mutant Associates with Lipid Bodies

... efflux of FC to HDL but the selective uptake of HDL-cholesteryl esters (Babitt et al., 1997). How cholesterol distributes to other intracellular membranes remains unknown, although the existence of lipid rafts not only on the plasma membrane but in some intracellular membranes has been suggested. Fo ...
Review Article GPCRs: Lipid-Dependent Membrane Receptors That
Review Article GPCRs: Lipid-Dependent Membrane Receptors That

... GPCRs are integral membrane proteins with multiple transmembrane passes. The interaction of membrane lipids with these receptors is therefore an important determinant in their structure and function [36, 41–44]. In addition, it has recently been reported that the interaction between GPCRs and G prot ...
Making Membranes in Artificial Chemistries
Making Membranes in Artificial Chemistries

... These particles self-assemble into membranes and protocells. Adapted from (Ono and Ikegami, 2002). Structurally, these membranes differ from natural membranes in that they readily form three-way junctions. This means that membranes tend to be shared between neighbouring protocells, and the protocell ...
Movement Through The cell New Notes
Movement Through The cell New Notes

... Video 1. The membrane encloses the cell & keeps it _______ inside. 2. Molecules are always on the ______. 3. Molecules move _____ a concentration gradient. 4. Is energy added during passive transport? 5. When the solution is low, the cell will ________. ...
Cell Membrane Properties
Cell Membrane Properties

... We’ll look at each of these factors in turn. ...
Biological membranes - Essays in Biochemistry
Biological membranes - Essays in Biochemistry

... (PC) (Figure 1a), which has a choline molecule attached to the phosphate group. Serine and ethanolamine can replace the choline in this position, and these lipids are called phosphatidylserine (PS) and phosphatidylethanolamine (PE), respectively. Phospholipids can also be sphingophospholipids (based ...
Renal tubular transp..
Renal tubular transp..

... Na+ absorbed by active transport mechanisms, NOT by TM mechanism. Basolateral ATPases establish a gradient across the tubule wall.  Proximal tubule is very permeable to Na+, so ions flow down gradient, across membranes.  Microvilli create large surface area for absorption.  Electrical gradient c ...
biological membranes - Biochemical Society
biological membranes - Biochemical Society

... composition and cholesterol content. At low temperatures, the hydrocarbon tails of bilayer lipids can pack closely together to form an ordered arrangement (or gel state) which is fairly rigid. As temperature is increased, the lipid molecules vibrate more rapidly, causing the bilayer to ‘melt’ into a ...
STRUCTURE-FUNCTION RELATIONSHIPS IN
STRUCTURE-FUNCTION RELATIONSHIPS IN

... encloses more than one droplet . Lipid droplets of this class have been readily identified in the roughly 1 in 20 cells sectioned through a region of thickened cytoplasm . Lipid droplets of the second class, characterized by . their lack of association with a fenestrated envelope, were observed with ...
PowerPoint Template
PowerPoint Template

... from the receptor to its downstream effector is usually a membrane-associated process. The example shown in the diagram above is only to be construed as an example for the composition of a generic signaling pathway. The structure of the intracellular signaling pathways of a cell are highly variable. ...
GUTS Lecture Syllabus for Lipid Structure and Nomenclature
GUTS Lecture Syllabus for Lipid Structure and Nomenclature

... cholesterol  is  usually  bonded  via  the  hydroxyl   Cholesterol  ester group  to  a  long-­‐chain  fatty  acid;  this  structure  is   called  a  cholesterol  ester  and  it  is  even  more  hydrophobic  than  cholesterol.    Cholesterol ...
The Plasma Membrane
The Plasma Membrane

... hydrophobic interior of the phospholipid bilayer. • Others are peripheral, meaning they lie on either side of the membrane but are not bound to its hydrophobic interior. ...
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Lipid raft



The plasma membranes of cells contain combinations of glycosphingolipids and protein receptors organized in glycolipoprotein microdomains termed lipid rafts. These specialized membrane microdomains compartmentalize cellular processes by serving as organizing centers for the assembly of signaling molecules, influencing membrane fluidity and membrane protein trafficking, and regulating neurotransmission and receptor trafficking. Lipid rafts are more ordered and tightly packed than the surrounding bilayer, but float freely in the membrane bilayer. Although more common in plasma membrane, lipid rafts have also been reported in other parts of the cell, such as Golgi and lysosomes.
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