Role of cholesterol in SNARE-mediated trafficking on intracellular
... membrane alongside the hydrocarbon chains of the surrounding lipids. Cholesterol is not uniformly distributed in the membrane, and besides its interaction with membrane lipids, interaction with proteins also have an important role in its distribution (Epand, 2006; Ikonen and Jansen, 2008). Consequen ...
... membrane alongside the hydrocarbon chains of the surrounding lipids. Cholesterol is not uniformly distributed in the membrane, and besides its interaction with membrane lipids, interaction with proteins also have an important role in its distribution (Epand, 2006; Ikonen and Jansen, 2008). Consequen ...
Synthetic membrane transporters J Middleton Boon and Bradley D
... translocases were reported in 1999 (Figure 3, 6–88) [29]. The sulfonamide derivative 6 enhances the inward translocation of a fluorescent phosphatidylcholine (PC) probe across surface differentiated vesicles. Amide 7 has only a minor effect, whereas control ester 8 fails to facilitate transport beca ...
... translocases were reported in 1999 (Figure 3, 6–88) [29]. The sulfonamide derivative 6 enhances the inward translocation of a fluorescent phosphatidylcholine (PC) probe across surface differentiated vesicles. Amide 7 has only a minor effect, whereas control ester 8 fails to facilitate transport beca ...
Lecture 10 Thurs 4-27-06
... B. Plasmodium-infected red blood cells (IRBCs): 1. Adhesion to vascular endothelium is a key factor in pathogenicity and is dependent on the Plasmodium protein PfEMP1 and endothelial receptors including CD36. 2. Evidence that binding of IRBCs to CD36 on endothelial cells activates a signaling pathwa ...
... B. Plasmodium-infected red blood cells (IRBCs): 1. Adhesion to vascular endothelium is a key factor in pathogenicity and is dependent on the Plasmodium protein PfEMP1 and endothelial receptors including CD36. 2. Evidence that binding of IRBCs to CD36 on endothelial cells activates a signaling pathwa ...
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... Eukaryotes are characterized by endomembranes that are connected by vesicular transport along secretory and endocytic pathways. The compositional differences between the various cellular membranes are maintained by sorting events, and it has long been believed that sorting is based solely on protein ...
... Eukaryotes are characterized by endomembranes that are connected by vesicular transport along secretory and endocytic pathways. The compositional differences between the various cellular membranes are maintained by sorting events, and it has long been believed that sorting is based solely on protein ...
Optical measurement of cell membrane tension
... inferring the absolute position of fluorescent dye molecules attached to the membrane from the absolute fluorescence intensity, which may limit both the sensitivity and acquisition rate of the technique20. Thus, none of these techniques is suitable for making spatially-resolved measurements of the d ...
... inferring the absolute position of fluorescent dye molecules attached to the membrane from the absolute fluorescence intensity, which may limit both the sensitivity and acquisition rate of the technique20. Thus, none of these techniques is suitable for making spatially-resolved measurements of the d ...
Transcription- and translation-dependent changes in membrane
... Cell cycle events have been proposed to be triggered by the formation of membrane domains in the process of coupled transcription, translation and insertion (‘transertion’) of nascent membrane and exported proteins. Disruption of domain structure should lead to changes in membrane dynamics. Membrane ...
... Cell cycle events have been proposed to be triggered by the formation of membrane domains in the process of coupled transcription, translation and insertion (‘transertion’) of nascent membrane and exported proteins. Disruption of domain structure should lead to changes in membrane dynamics. Membrane ...
Hemojuvelin N-terminal mutants reach the plasma membrane but do
... 0.05% glutaraldehyde at different time points (6, 12 and 18 hours), labeled with goat polyclonal anti-cMYC (Novus Biological, Littelton, CO, USA) using the goldenhance protocol, embedded in Epon-812, and cut as described previously.11,14 Electron microscope images were acquired from thin sections un ...
... 0.05% glutaraldehyde at different time points (6, 12 and 18 hours), labeled with goat polyclonal anti-cMYC (Novus Biological, Littelton, CO, USA) using the goldenhance protocol, embedded in Epon-812, and cut as described previously.11,14 Electron microscope images were acquired from thin sections un ...
Chapter 5
... are not all identical, they share the same fundamental architecture. Cell membranes are assembled from four components (table 5.1): 1. Phospholipid bilayer. Every cell membrane is composed of phospholipids in a bilayer. The other components of the membrane are embedded within the bilayer, which pr ...
... are not all identical, they share the same fundamental architecture. Cell membranes are assembled from four components (table 5.1): 1. Phospholipid bilayer. Every cell membrane is composed of phospholipids in a bilayer. The other components of the membrane are embedded within the bilayer, which pr ...
Presence of exposed phospholipids in the outer membrane of Vibrio
... Sensitivity to hydrophobic compounds and detergents suggested that the outer membrane of V. cholerae might not be an effective barrier to such compounds. This was confirmed by the extensive lysis (about 80%) of these cells following treatment with 0.01% SDS, sodium deoxycholate and Triton X-100. The ...
... Sensitivity to hydrophobic compounds and detergents suggested that the outer membrane of V. cholerae might not be an effective barrier to such compounds. This was confirmed by the extensive lysis (about 80%) of these cells following treatment with 0.01% SDS, sodium deoxycholate and Triton X-100. The ...
Erratum to: Minimization of extracellular space as a driving force in
... (“the two models” portion of the comment). We don’t understand what difference this distinction makes for the discussion of the Martin and Müller article, unless the reviewer is arguing for a metabolic symbiosis in which the proto-mitochondrion contributed a respiratory, as opposed to a fermentative ...
... (“the two models” portion of the comment). We don’t understand what difference this distinction makes for the discussion of the Martin and Müller article, unless the reviewer is arguing for a metabolic symbiosis in which the proto-mitochondrion contributed a respiratory, as opposed to a fermentative ...
Phosphatidylcholine traffic to the vacuole
... and significance of non-random lipid distributions within cells, the dynamic processes involved in generating and maintaining this level of organization remain poorly understood. Historically, fractionation studies have proven informative by establishing the steady-state distribution of lipids both ...
... and significance of non-random lipid distributions within cells, the dynamic processes involved in generating and maintaining this level of organization remain poorly understood. Historically, fractionation studies have proven informative by establishing the steady-state distribution of lipids both ...
Beyond ergosterol
... (with mannitol).26 Fluconazole treatment and ERG3 deletion have both been shown to inhibit filamentation,28,29 which can now be explained, at least in part, due to impaired V-ATPase function. The ability to transition from yeast to hyphae is a critical virulence factor for polymorphic pathogenic fun ...
... (with mannitol).26 Fluconazole treatment and ERG3 deletion have both been shown to inhibit filamentation,28,29 which can now be explained, at least in part, due to impaired V-ATPase function. The ability to transition from yeast to hyphae is a critical virulence factor for polymorphic pathogenic fun ...
Encapsulation Services
... high concentrations of bioactives in the target cells and cellular compartments in order to gain maximum therapeutic efficiency. Active targeting can be achieved via appropriately engineered modifications to the liposomal structure. For active targeting of liposomes, thermo-labile, pH-sensitive, p ...
... high concentrations of bioactives in the target cells and cellular compartments in order to gain maximum therapeutic efficiency. Active targeting can be achieved via appropriately engineered modifications to the liposomal structure. For active targeting of liposomes, thermo-labile, pH-sensitive, p ...
active transport
... couples the transport of two solutes • A single ATP-powered pump that transports one solute can indirectly drive the active transport of several other solutes through cotransport via a different protein. • As the solute that has been actively transported diffuses back passively through a transport p ...
... couples the transport of two solutes • A single ATP-powered pump that transports one solute can indirectly drive the active transport of several other solutes through cotransport via a different protein. • As the solute that has been actively transported diffuses back passively through a transport p ...
PDF - Walter Lab
... requirements1–5. To mediate endocytosis, effectors assemble transiently underneath the plasma membrane, carry out the mechanics of membrane deformation, cargo selection and vesicle internalization, and then disassemble. The mechanism by which endocytosis initiates at particular locations on the plas ...
... requirements1–5. To mediate endocytosis, effectors assemble transiently underneath the plasma membrane, carry out the mechanics of membrane deformation, cargo selection and vesicle internalization, and then disassemble. The mechanism by which endocytosis initiates at particular locations on the plas ...
Membrane
... • Later studies found problems with this model, particularly the placement of membrane proteins, which have hydrophilic and hydrophobic regions • In 1972, J. Singer and G. Nicolson proposed that the membrane is a mosaic of proteins dispersed within the bilayer, with only the hydrophilic regions expo ...
... • Later studies found problems with this model, particularly the placement of membrane proteins, which have hydrophilic and hydrophobic regions • In 1972, J. Singer and G. Nicolson proposed that the membrane is a mosaic of proteins dispersed within the bilayer, with only the hydrophilic regions expo ...
Untitled
... pass through the nuclear pore and can therefore cross while maintaining their higher order 3-dimensional shape. In contrast to nuclear transport, proteins that move into chloroplasts, the ER, and mitochondria must do so in an unfolded state, i.e. as linear chains of amino acids. The different channe ...
... pass through the nuclear pore and can therefore cross while maintaining their higher order 3-dimensional shape. In contrast to nuclear transport, proteins that move into chloroplasts, the ER, and mitochondria must do so in an unfolded state, i.e. as linear chains of amino acids. The different channe ...
2/4 Lipids
... saturated fats), because they’re solid, can clog up an artery like a traffic jam, preventing blood from flowing through. Unsaturated fats, being liquids, don’t do that. – Saturated fats in their rigid regular structures are also harder to break down. So, the body tends to break up and use unsaturate ...
... saturated fats), because they’re solid, can clog up an artery like a traffic jam, preventing blood from flowing through. Unsaturated fats, being liquids, don’t do that. – Saturated fats in their rigid regular structures are also harder to break down. So, the body tends to break up and use unsaturate ...
Cell Membranes
... Should the inside of the cell be more negative or more positive than the outside of the cell? If there is a glycocalyx (sugar bundle) on the outside of the cell, what does it do to the charge on the OUTSIDE of the cell membrane? What are the three types of Carbohydrates? What are the functions of th ...
... Should the inside of the cell be more negative or more positive than the outside of the cell? If there is a glycocalyx (sugar bundle) on the outside of the cell, what does it do to the charge on the OUTSIDE of the cell membrane? What are the three types of Carbohydrates? What are the functions of th ...
Chapter # PLASMA MEMBRANE PHOSPHOLIPID ASYMMETRY
... ATP binding site and a phosphoenzyme formation site are located within the largest cytosolic loop, whereas a sequence implicated in the coupling to transport activity was identified in another hydrophilic loop. The sequence of ...
... ATP binding site and a phosphoenzyme formation site are located within the largest cytosolic loop, whereas a sequence implicated in the coupling to transport activity was identified in another hydrophilic loop. The sequence of ...
examination by electron microscopy of the interaction between
... cytoplasm are associated with vacuoles in which the limiting membrane is more tightly apposed to the ingested organism leaving only a relatively narrow, intervening peribacillary space. This space is filled with a dense, finely granular, acid phosphatase-positive material identical in appearance to ...
... cytoplasm are associated with vacuoles in which the limiting membrane is more tightly apposed to the ingested organism leaving only a relatively narrow, intervening peribacillary space. This space is filled with a dense, finely granular, acid phosphatase-positive material identical in appearance to ...
Analysis of Protein Interactions at Native Chloroplast Membranes by
... and mycotoxins with detection levels as low as 0.1 ng/ml [6]. TIRE has also been used to determine the binding affinity of the molecular chaperone Hsp70 for a soluble form of its receptor OEP61 bound to a gold surface, and was sensitive enough to discriminate between the binding affinities of closel ...
... and mycotoxins with detection levels as low as 0.1 ng/ml [6]. TIRE has also been used to determine the binding affinity of the molecular chaperone Hsp70 for a soluble form of its receptor OEP61 bound to a gold surface, and was sensitive enough to discriminate between the binding affinities of closel ...
The Membrane Skeleton of a Unicellular Consists
... powder (5- or 25-urn diam, Heat Systems-Ultrasonics, Inc., Plainview, NY) to facilitate cell breakage. Two 10-s cavitations at the No. 4 setting of a Branson X125 Sonifier (Branson Instruments, Danbury, CT) separated by a short cooling interval resulted in complete cell disruption. Glass powder was ...
... powder (5- or 25-urn diam, Heat Systems-Ultrasonics, Inc., Plainview, NY) to facilitate cell breakage. Two 10-s cavitations at the No. 4 setting of a Branson X125 Sonifier (Branson Instruments, Danbury, CT) separated by a short cooling interval resulted in complete cell disruption. Glass powder was ...
Lipid raft
The plasma membranes of cells contain combinations of glycosphingolipids and protein receptors organized in glycolipoprotein microdomains termed lipid rafts. These specialized membrane microdomains compartmentalize cellular processes by serving as organizing centers for the assembly of signaling molecules, influencing membrane fluidity and membrane protein trafficking, and regulating neurotransmission and receptor trafficking. Lipid rafts are more ordered and tightly packed than the surrounding bilayer, but float freely in the membrane bilayer. Although more common in plasma membrane, lipid rafts have also been reported in other parts of the cell, such as Golgi and lysosomes.