Anti-CD30 human IL-2 fusion proteins display strong and specific
... Although therapy of CD30-positive lymphomas such as classical Hodgkin lymphoma and anaplastic large cell lymphoma has been improved considerably during the last decades, patients suffer from high toxicity of current therapeutic regimens. Since CD30 expression is very restricted, CD30-positive tumors ...
... Although therapy of CD30-positive lymphomas such as classical Hodgkin lymphoma and anaplastic large cell lymphoma has been improved considerably during the last decades, patients suffer from high toxicity of current therapeutic regimens. Since CD30 expression is very restricted, CD30-positive tumors ...
Macromolecules
... Macromolecules are formed when monomers are linked together to form longer chains called polymers. The same process of making and breaking polymers is found in all living organisms. ...
... Macromolecules are formed when monomers are linked together to form longer chains called polymers. The same process of making and breaking polymers is found in all living organisms. ...
Structural analysis of bacterial virulence factors
... activity of CagA fragments (e.g. effect on cell morphology and motility, interaction with various partner molecules) will be assessed. We are also interested in investigating the structure/function relationships in VacA – a multifunctional H. pylori toxin that induces a variety of cytopathic effects ...
... activity of CagA fragments (e.g. effect on cell morphology and motility, interaction with various partner molecules) will be assessed. We are also interested in investigating the structure/function relationships in VacA – a multifunctional H. pylori toxin that induces a variety of cytopathic effects ...
GFP
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin antibody. Bound peroxidase is ...
... into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and a peroxidase-coupled anti-digoxigenin antibody. Bound peroxidase is ...
Pholem-specific promoter used to express resistance gene
... under the control of the AtSUC2-940 promoter have wild type phenotypes. Although the AtSUC2 promoter has been reported to be phloem-specific, we have found that it often does not maintain this tissue-specific pattern of expression in transformed Arabidopsis. However, despite the likelihood of expres ...
... under the control of the AtSUC2-940 promoter have wild type phenotypes. Although the AtSUC2 promoter has been reported to be phloem-specific, we have found that it often does not maintain this tissue-specific pattern of expression in transformed Arabidopsis. However, despite the likelihood of expres ...
Pharmaceutical re-activation of pathways in non
... chemotherapeutic agent is in fact the p53-null group versus the p53-wild type. This could probably be easily added in to the experiment at some point. We also have no method built-in presently to count the actually number of cells being lysed and put into the protein array. It is possible that the d ...
... chemotherapeutic agent is in fact the p53-null group versus the p53-wild type. This could probably be easily added in to the experiment at some point. We also have no method built-in presently to count the actually number of cells being lysed and put into the protein array. It is possible that the d ...
N E W S A N D ... a b
... could come directly from having few protein copies, but because low concentrations are hard to measure, this has not yet been explicitly demonstrated. It could also be transmitted from having few transcripts1,8–10, random activation-inactivation of the genes3,4,11 or fluctuations in any other factor ...
... could come directly from having few protein copies, but because low concentrations are hard to measure, this has not yet been explicitly demonstrated. It could also be transmitted from having few transcripts1,8–10, random activation-inactivation of the genes3,4,11 or fluctuations in any other factor ...
Gen660_Lecture9B_GeneExpressionEvo_2014
... From Pal et al. Integrated View of Protein Evolution ...
... From Pal et al. Integrated View of Protein Evolution ...
Worksheet6-3Proteins
... 10. To make all the proteins your body needs, you require ________________ different amino acids. 11. Why are some amino acids called “non-essential” amino acids, even when your body still needs them? _______________________________________________________________ ___________________________________ ...
... 10. To make all the proteins your body needs, you require ________________ different amino acids. 11. Why are some amino acids called “non-essential” amino acids, even when your body still needs them? _______________________________________________________________ ___________________________________ ...
Ch 5
... Amino acids are transported to the mRNA by transfer RNA (tRNA). mRNA has codons – a sequence of 3 nucleotides that codes for an amino acid. tRNA has anticodons that are complementary to mRNA’s codons. AUG is the universal ‘start’ codon that tells the ribosome to start translating. There are three ‘s ...
... Amino acids are transported to the mRNA by transfer RNA (tRNA). mRNA has codons – a sequence of 3 nucleotides that codes for an amino acid. tRNA has anticodons that are complementary to mRNA’s codons. AUG is the universal ‘start’ codon that tells the ribosome to start translating. There are three ‘s ...
Tentative exam questions on Food Biochemistry part - e
... Define gelation as a functional property of proteins Thermally reversible and irreversible gels Influence of pH on protein gel properties What is emulsion? Define emulsification capacity and stability Why can proteins serve as emulsifying agents? Any important protein features? Utilization of prote ...
... Define gelation as a functional property of proteins Thermally reversible and irreversible gels Influence of pH on protein gel properties What is emulsion? Define emulsification capacity and stability Why can proteins serve as emulsifying agents? Any important protein features? Utilization of prote ...
Ch 13
... Signal Transduction • Signal from exterior of cell must affect interior of cell • Through the membrane • Amplification of signal • Ability to turn off ...
... Signal Transduction • Signal from exterior of cell must affect interior of cell • Through the membrane • Amplification of signal • Ability to turn off ...
Protein Purification under Native Conditions
... i. When the expression level is high, 2 washed are usually sufficient for removal of contaminants. ii. For very low expression levels of highly concentrated lysates, 3 wash steps may be required to achieve high purity. 21. Save the flow-through (wash fractions) for analysis by SDS-PAGE to check the ...
... i. When the expression level is high, 2 washed are usually sufficient for removal of contaminants. ii. For very low expression levels of highly concentrated lysates, 3 wash steps may be required to achieve high purity. 21. Save the flow-through (wash fractions) for analysis by SDS-PAGE to check the ...
Document
... mRNA: a copy of gene; with exactly the same sequence as one of the strands of the gene but substituting U for T Introns (内含子): parts of a gene / not used in protein synthesis; spliced out from mRNA>shortened mRNA leaves nucleus with exons (外 显子) plus regulatory region ...
... mRNA: a copy of gene; with exactly the same sequence as one of the strands of the gene but substituting U for T Introns (内含子): parts of a gene / not used in protein synthesis; spliced out from mRNA>shortened mRNA leaves nucleus with exons (外 显子) plus regulatory region ...
long - David Pollock
... structural comparison and prediction, biochemical adaptation, evolution of protein complexes, probabilistic methods for detecting patterns of sequence evolution, effects of population structure on protein evolution, lattice and other computational models of protein evolution, protein folding and ene ...
... structural comparison and prediction, biochemical adaptation, evolution of protein complexes, probabilistic methods for detecting patterns of sequence evolution, effects of population structure on protein evolution, lattice and other computational models of protein evolution, protein folding and ene ...
REGULATION OF PROTEIN SYNTHESIS I. Bacteria
... looks smooth in cultures, individual cells vary greatly-some have induced mRNA, some don’t; some have a lot of protein, some don’t. mRNA and protein levels don’t correlate, because the mRNA degrades much more rapidly than the protein (so amount of protein represents the mRNA history, not presence). ...
... looks smooth in cultures, individual cells vary greatly-some have induced mRNA, some don’t; some have a lot of protein, some don’t. mRNA and protein levels don’t correlate, because the mRNA degrades much more rapidly than the protein (so amount of protein represents the mRNA history, not presence). ...
Protein Expression Issues in Protein Expression
... Expression systems • Expression systems – E. coli – Insect cell line Sf9 – Yeast (Pichia pastoris) – Other cell lines. ...
... Expression systems • Expression systems – E. coli – Insect cell line Sf9 – Yeast (Pichia pastoris) – Other cell lines. ...
Document
... molecule • Used to bind biotinylated nucleic acids or peptides McDevitt, 1999 Big idea: Streptavidin protein expressed on the cell surface can be used to target any biotinylated DNA/protein to the cell surface. ...
... molecule • Used to bind biotinylated nucleic acids or peptides McDevitt, 1999 Big idea: Streptavidin protein expressed on the cell surface can be used to target any biotinylated DNA/protein to the cell surface. ...
Serrétia is made up of pure pharmaceutical grade
... Silk is among the strongest natural proteins on earth. Silkworm larvae excrete the serrapeptase enzyme to dissolve their silk cocoons. Serrapeptase miraculously dissolves the tough silk protein fibers of the silk cocoon, yet has no effect on the silkworm’s living tissue. The powerful protein-dissolv ...
... Silk is among the strongest natural proteins on earth. Silkworm larvae excrete the serrapeptase enzyme to dissolve their silk cocoons. Serrapeptase miraculously dissolves the tough silk protein fibers of the silk cocoon, yet has no effect on the silkworm’s living tissue. The powerful protein-dissolv ...
Using a Mechanistic Perspective to Simulate Protein Backbone Motion
... motion without the need to calculate all intermolecular forces. As a first step towards such a computationally efficient method, we use operational space control principles from robotics and kinematics principles from mechanics to simulate the motion of a protein's backbone at interactive rates. CSE ...
... motion without the need to calculate all intermolecular forces. As a first step towards such a computationally efficient method, we use operational space control principles from robotics and kinematics principles from mechanics to simulate the motion of a protein's backbone at interactive rates. CSE ...
Guillain-Barre syndrome (GBS)
... for initial transfer to the ER: with a signal sequence at N-terminus; consisting of 5-10 hydrophobic aa Go forward Golgi: most proteins Return to ER (ER residents): with a specific sequence of 4 aa at C-terminus Go to mitochondria: positively charged amino acids alternate with hydrophobic ones ...
... for initial transfer to the ER: with a signal sequence at N-terminus; consisting of 5-10 hydrophobic aa Go forward Golgi: most proteins Return to ER (ER residents): with a specific sequence of 4 aa at C-terminus Go to mitochondria: positively charged amino acids alternate with hydrophobic ones ...
Chongqing Biospes Co., Ltd - Antibodies, Proteins, ELISA kits and
... precipitate, leave very small volume of supernatant to avoid touching.) 10. For precipitate: discard the supernatant, add 50 μl of NER (containing PMSF) to the precipitate. (Discard the supernatant thoroughly to avoid contamination of cytoplasmic proteins.) 11. Vortex at maximum speed for 15-30 seco ...
... precipitate, leave very small volume of supernatant to avoid touching.) 10. For precipitate: discard the supernatant, add 50 μl of NER (containing PMSF) to the precipitate. (Discard the supernatant thoroughly to avoid contamination of cytoplasmic proteins.) 11. Vortex at maximum speed for 15-30 seco ...
Regulatory role of hsa-miR-939 on pro
... Transfection of miR-939 resulted in the reduction of IL-6 and NOS2A mRNAs, a decrease in protein levels of IL-6, VEGFA, NOS2A, and an abrogation of NFκB activity. Collectively, our data suggests that downregulation of miR-939 in CRPS patients may enhance target gene expression involved in inflammato ...
... Transfection of miR-939 resulted in the reduction of IL-6 and NOS2A mRNAs, a decrease in protein levels of IL-6, VEGFA, NOS2A, and an abrogation of NFκB activity. Collectively, our data suggests that downregulation of miR-939 in CRPS patients may enhance target gene expression involved in inflammato ...
Single particle cryo-EM of membrane proteins in lipid nanodisc
... In the last few years, major technological breakthroughs enabled single particle cryo-EM to become the technique of choice for structure determination of many challenging biological macromolecules. Atomic structures of many membrane proteins that are refractory to crystallization have now determined ...
... In the last few years, major technological breakthroughs enabled single particle cryo-EM to become the technique of choice for structure determination of many challenging biological macromolecules. Atomic structures of many membrane proteins that are refractory to crystallization have now determined ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.