Tertiary Structure
... 2). The 3 major classes of 3o structure are fibrous proteins, globular proteins, and membrane proteins. 3). Fibrous proteins are hydrophobic proteins that give strength and flexibility. 4). Coiled-coils are stabilized by hydrophobic interactions. 5). Globular proteins constitute the majority of prot ...
... 2). The 3 major classes of 3o structure are fibrous proteins, globular proteins, and membrane proteins. 3). Fibrous proteins are hydrophobic proteins that give strength and flexibility. 4). Coiled-coils are stabilized by hydrophobic interactions. 5). Globular proteins constitute the majority of prot ...
Structural basics of human muscle fructose-1,6
... molecular basis for a mechanism of their action is not clear. The aim of my Ph.D. thesis was to investigate the structure of human muscle FBPase. In the cause of studies I solved eight crystal structures of the muscle enzyme in its various states. On the basis of the structures of inactive FBPase in ...
... molecular basis for a mechanism of their action is not clear. The aim of my Ph.D. thesis was to investigate the structure of human muscle FBPase. In the cause of studies I solved eight crystal structures of the muscle enzyme in its various states. On the basis of the structures of inactive FBPase in ...
PPR (pentatricopeptide repeat) proteins in mammals: important aids
... proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target ...
... proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target ...
Cellular Event Cards
... The developing red blood cell must make a large amount of hemoglobin. Attach two green activators to the gene for hemoglobin to indicate that this gene must be highly expressed. ...
... The developing red blood cell must make a large amount of hemoglobin. Attach two green activators to the gene for hemoglobin to indicate that this gene must be highly expressed. ...
Biochemistry notes (updated 10/13)
... Phospholipids have both polar and nonpolar sections. As a result, they are able to dissolve in both type of solvents as well. They are important for living things because they form the borders of all cells (cell membranes) and also participate in forming many cell organelles. ...
... Phospholipids have both polar and nonpolar sections. As a result, they are able to dissolve in both type of solvents as well. They are important for living things because they form the borders of all cells (cell membranes) and also participate in forming many cell organelles. ...
Leukaemia Section t(11;22)(q23;q13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Chimeric mRNAs from both derivative chromosomes are found. ...
... Chimeric mRNAs from both derivative chromosomes are found. ...
Leukaemia Section t(8;17)(q24;q22) ???BCL3/MYC Atlas of Genetics and Cytogenetics
... Cytogenetics Cytogenetics morphological The karyotype also showed the classical t(14;18)(q32;q21), usually found in follicular lymphoma, a 12q+ and a Xp+, not otherwise described. ...
... Cytogenetics Cytogenetics morphological The karyotype also showed the classical t(14;18)(q32;q21), usually found in follicular lymphoma, a 12q+ and a Xp+, not otherwise described. ...
Protein and proteome analytics
... regulatory processes. Depending on the species, the proteome can comprise up to 1,000,000 protein species and thus nor- ...
... regulatory processes. Depending on the species, the proteome can comprise up to 1,000,000 protein species and thus nor- ...
Protein Expression and Purification
... Well suited for proteins to be used for Ab production, structural, functional studies ...
... Well suited for proteins to be used for Ab production, structural, functional studies ...
Gene Regulation -
... transcribe mRNA. Although all promoters have the same function and share similar sequences that are recognized by RNA polymerase, they differ enough so that some are very strong (leading to high levels of transcription) and others are weak (rarely transcribed). Thus, one level of regulating gene exp ...
... transcribe mRNA. Although all promoters have the same function and share similar sequences that are recognized by RNA polymerase, they differ enough so that some are very strong (leading to high levels of transcription) and others are weak (rarely transcribed). Thus, one level of regulating gene exp ...
Gene Section AFF1 (AF4/FMR2 family, member 1) Atlas of Genetics and Cytogenetics
... splice of exon 18 to the 3'-NTR, skipping exon 19 and 20. Therefore this protein comes in different flavors, as there are presumably three independent promotor, and one carboxy-terminal exon skipping. Bernard OA, Berger R. Molecular basis of 11q23 rearrangements in hematopoietic malignant proliferat ...
... splice of exon 18 to the 3'-NTR, skipping exon 19 and 20. Therefore this protein comes in different flavors, as there are presumably three independent promotor, and one carboxy-terminal exon skipping. Bernard OA, Berger R. Molecular basis of 11q23 rearrangements in hematopoietic malignant proliferat ...
Proceeding - ETH Zürich
... researchers [3-5] have investigated the interactions between non-ionic solutes and ionic polymers, such as sugar and proteins. It was demonstrated that in the presence of sugars the proteins are preferentially hydrated. Consequently, sugars are preferentially excluded from contact with the surface o ...
... researchers [3-5] have investigated the interactions between non-ionic solutes and ionic polymers, such as sugar and proteins. It was demonstrated that in the presence of sugars the proteins are preferentially hydrated. Consequently, sugars are preferentially excluded from contact with the surface o ...
posterexample1
... mechanically, the fatty acid linolenic acid (18:3) is metabolized to produce the plant hormone, jasmonic acid (JA), which accumulates to high levels in wounded tissues. The JA pathway is initiated in the chloroplasts and completed in the peroxisomes. JA is then exported to the cytoplasm where it is ...
... mechanically, the fatty acid linolenic acid (18:3) is metabolized to produce the plant hormone, jasmonic acid (JA), which accumulates to high levels in wounded tissues. The JA pathway is initiated in the chloroplasts and completed in the peroxisomes. JA is then exported to the cytoplasm where it is ...
Protein folding and structure
... 12. Ribonuclease structure is stabilized by ∆G = -7.1 kJ/mol at pH 2.5 and T = 25 ºC. What is the ratio between folded and denatured molecules? The enthalphy for folding is 238.6 kJ/mol at 25 ºC. Assume that enthalpy and entropy do not change when temperature is raised to 37 ºC. What is ∆G at 37 ºC ...
... 12. Ribonuclease structure is stabilized by ∆G = -7.1 kJ/mol at pH 2.5 and T = 25 ºC. What is the ratio between folded and denatured molecules? The enthalphy for folding is 238.6 kJ/mol at 25 ºC. Assume that enthalpy and entropy do not change when temperature is raised to 37 ºC. What is ∆G at 37 ºC ...
Plant basal defenses 1. Pre-existing 2. Induced Pre
... • Physical barriers involve properties of the plant surface, that is, the cuticle, stomata and cell walls. • Chemical barriers include compounds, such as phytoanticipins that have antimicrobial activity, and defensins, which interfere with pathogen nutrition and retard their development. ...
... • Physical barriers involve properties of the plant surface, that is, the cuticle, stomata and cell walls. • Chemical barriers include compounds, such as phytoanticipins that have antimicrobial activity, and defensins, which interfere with pathogen nutrition and retard their development. ...
Proteomic Survey of Camel Urine Reveals High Levels of
... camelid sequences. Additionally, peptides identified from camel EST sequences were subjected to short BLASTP searches, to check that both peptide and EST sequence could be matched to the same protein description. These descriptions should be treated with caution ...
... camelid sequences. Additionally, peptides identified from camel EST sequences were subjected to short BLASTP searches, to check that both peptide and EST sequence could be matched to the same protein description. These descriptions should be treated with caution ...
Biology 102, Lectures 17 and 18 Study Guide
... 10. During the process of transcription, does the DNA molecule unwind completely? 11. What are the functions of RNA polymerase during transcription? 12. Once synthesized, how is mRNA further modified prior to leaving the nucleus? What are the functions of this modification (one known, one more specu ...
... 10. During the process of transcription, does the DNA molecule unwind completely? 11. What are the functions of RNA polymerase during transcription? 12. Once synthesized, how is mRNA further modified prior to leaving the nucleus? What are the functions of this modification (one known, one more specu ...
Alignment of pairs of sequences
... Why compare sequences? • To find whether two (or more) genes or proteins are evolutionarily related to each other • To find structurally or functionally similar regions within proteins ...
... Why compare sequences? • To find whether two (or more) genes or proteins are evolutionarily related to each other • To find structurally or functionally similar regions within proteins ...
Protein: On the Scene
... Scientists have found 20 different amino acids in protein, and these 20 amino acids can combine in lots of ways - in fact, they have joined together to make thousands of different proteins! Some types of amino acids are made by you, right inside your body, without you ever thinking about it or doing ...
... Scientists have found 20 different amino acids in protein, and these 20 amino acids can combine in lots of ways - in fact, they have joined together to make thousands of different proteins! Some types of amino acids are made by you, right inside your body, without you ever thinking about it or doing ...
B-PERfusions
... B-PER and fusion proteins time the pellets were uniformly dispersed in the lysis solution. From this point, the IPIII-His and MBP fusion lysates were processed differently, as detailed in the next two paragraphs. Purification of His-tagged fusion proteins To each IPIII-His fusion lysate was added 1 ...
... B-PER and fusion proteins time the pellets were uniformly dispersed in the lysis solution. From this point, the IPIII-His and MBP fusion lysates were processed differently, as detailed in the next two paragraphs. Purification of His-tagged fusion proteins To each IPIII-His fusion lysate was added 1 ...
PureCube Rho1D4 Agarose
... One advantage of the system is the high specificity of the antibody-epitope interaction. Epitope sequence and chain length are critical for binding. For example, replacing the third alanine with glycine which removes a single methyl group, eliminates binding. Likewise, the full 9-amino acid tag bind ...
... One advantage of the system is the high specificity of the antibody-epitope interaction. Epitope sequence and chain length are critical for binding. For example, replacing the third alanine with glycine which removes a single methyl group, eliminates binding. Likewise, the full 9-amino acid tag bind ...
Planet Earth and Its Environment A 5000
... 300.) One or more polypeptides can be twisted together into a particular shape, resulting in the overall structure of a protein. www.answers.com ...
... 300.) One or more polypeptides can be twisted together into a particular shape, resulting in the overall structure of a protein. www.answers.com ...
Genetic Mutation Worksheet
... «DQGVRPHWLPHVEHFDXVH there is more than one codon for each amino acid, these mutations can be SI L E N T: ...
... «DQGVRPHWLPHVEHFDXVH there is more than one codon for each amino acid, these mutations can be SI L E N T: ...
Supplemental Table 2: Candidate gene criteria case example Gene
... Boumil RM, Letts VA, Roberts MC, et al. A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice. PLoS genetics. Aug 2010;6(8). Nakata T, Iwamoto A, Noda Y, Takemura R, Yoshikura H, Hirokawa N. Predominant and developmentally regulated expression of dynami ...
... Boumil RM, Letts VA, Roberts MC, et al. A missense mutation in a highly conserved alternate exon of dynamin-1 causes epilepsy in fitful mice. PLoS genetics. Aug 2010;6(8). Nakata T, Iwamoto A, Noda Y, Takemura R, Yoshikura H, Hirokawa N. Predominant and developmentally regulated expression of dynami ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.