ces-1 (cG-17): sc-12177 - Santa Cruz Biotechnology
... Several proteins involved in regulating and executing programmed cell death have been identified in C. elegans. CED-2, which is similar to the human adaptor protein CrkII, as well as CED-5 and CED-7, which are orthologs of the mammalian DOCK180 and ABC transporter proteins, respectively, are involve ...
... Several proteins involved in regulating and executing programmed cell death have been identified in C. elegans. CED-2, which is similar to the human adaptor protein CrkII, as well as CED-5 and CED-7, which are orthologs of the mammalian DOCK180 and ABC transporter proteins, respectively, are involve ...
AS 2.1.1 Protein Structure
... To calculate the total number of different possibilities, we need to multiply the total number of possibilities at each point. In this case: 20 x 20 x 20 = 8000 This means that 8000 different sequences of four amino acids are possible- 8000 different proteins can be made. Given that most proteins ar ...
... To calculate the total number of different possibilities, we need to multiply the total number of possibilities at each point. In this case: 20 x 20 x 20 = 8000 This means that 8000 different sequences of four amino acids are possible- 8000 different proteins can be made. Given that most proteins ar ...
Gene Section RSF1 (remodeling and spacing factor 1) in Oncology and Haematology
... which contains 1431, 1400, and 1189 amino acids, respectively. By characterizing a protein remodeling complex RSF, Loyola A et al has identified two interacting subunits: RSF1 and SNF2H. They used peptide sequence information of RSF1 to clone the fulllength cDNA. The deduced sequence contains 1441 a ...
... which contains 1431, 1400, and 1189 amino acids, respectively. By characterizing a protein remodeling complex RSF, Loyola A et al has identified two interacting subunits: RSF1 and SNF2H. They used peptide sequence information of RSF1 to clone the fulllength cDNA. The deduced sequence contains 1441 a ...
Gene Expression
... • A few can be harmful and then some are good – Harmful mutations can cause cancer and genetic disorders – Good mutation can make altered proteins which may be beneficial in different/changing environments ...
... • A few can be harmful and then some are good – Harmful mutations can cause cancer and genetic disorders – Good mutation can make altered proteins which may be beneficial in different/changing environments ...
PDF 52.16 KB
... Preliminary studies were also carried out on the feasibility of using two dimensional electrophoresis as a method for the detection of human growth hormone (hGH). There was question as to whether the high turnover and variability of the endogenous hormone make the detection of the actual administere ...
... Preliminary studies were also carried out on the feasibility of using two dimensional electrophoresis as a method for the detection of human growth hormone (hGH). There was question as to whether the high turnover and variability of the endogenous hormone make the detection of the actual administere ...
The QIAexpressionist™
... different expression systems due to the presence of the T5 promoter/lac operator transcription–translation system for expression in E. coli; the p10 promoter for baculovirusbased expression in insect cells; and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. The pQE-TriSystem ...
... different expression systems due to the presence of the T5 promoter/lac operator transcription–translation system for expression in E. coli; the p10 promoter for baculovirusbased expression in insect cells; and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. The pQE-TriSystem ...
Apresentação do PowerPoint
... sample. (A) The sample is loaded and voltage is applied. The proteins will migrate to their isoelectric pH, the location at which they have no net charge. (B) The proteins form bands that can be excised and used for further experimentation. ...
... sample. (A) The sample is loaded and voltage is applied. The proteins will migrate to their isoelectric pH, the location at which they have no net charge. (B) The proteins form bands that can be excised and used for further experimentation. ...
ppt
... Chapt 8 Student learning outcomes Because proteins are the active players in most cell processes ...
... Chapt 8 Student learning outcomes Because proteins are the active players in most cell processes ...
Gene Section TACC1 (transforming, acidic coiled-coil containing protein 1)
... Note The gene name TACC1, for Transforming Acidic Coiled coil containing was derived based on the initial finding that this gene could transform murine fibroblasts and is found in a chromosomal region amplified in breast cancer (Still et al., 1999). Studies in transgenic mice have demonstrated that ...
... Note The gene name TACC1, for Transforming Acidic Coiled coil containing was derived based on the initial finding that this gene could transform murine fibroblasts and is found in a chromosomal region amplified in breast cancer (Still et al., 1999). Studies in transgenic mice have demonstrated that ...
Confocal Fluorescence Microscopy
... A confocal fluorescence microscope is a serial rather than parallel imageacquisition device: the object is illuminated point by point and the generated fluorescence, imaged onto the detection pinhole, is measured sequentially for each illuminated point. In such an instrument, the image acquisition is ...
... A confocal fluorescence microscope is a serial rather than parallel imageacquisition device: the object is illuminated point by point and the generated fluorescence, imaged onto the detection pinhole, is measured sequentially for each illuminated point. In such an instrument, the image acquisition is ...
Chapter 3 Section 4 Protein Synthesis
... • The new strand of mRNA are made by matching new nitrogen bases with the exposed nitrogen bases from the unzipped strand of DNA. ...
... • The new strand of mRNA are made by matching new nitrogen bases with the exposed nitrogen bases from the unzipped strand of DNA. ...
Exam 2 Student Key
... LacI gene mutation. Each type of mutation will be used once. sequence 2 Repressor protein A: has normal protein sequence 2. silent mutation in 4th codon 3 Repressor protein B: cannot bind lactose, but it 3. Single amino acid change can bind the operator (missense) 1 Repressor protein C: binds neithe ...
... LacI gene mutation. Each type of mutation will be used once. sequence 2 Repressor protein A: has normal protein sequence 2. silent mutation in 4th codon 3 Repressor protein B: cannot bind lactose, but it 3. Single amino acid change can bind the operator (missense) 1 Repressor protein C: binds neithe ...
A short guided tour through functional and structural features of
... activator-like effect of this domain and perhaps additionally an influence on enzyme stability [71]. The rapidly increasing mass of sequence data of growing genome projects reveal an unanticipated number of SAPLIP sequences in various organisms. Since the definite function of a SAPLIP is not deducib ...
... activator-like effect of this domain and perhaps additionally an influence on enzyme stability [71]. The rapidly increasing mass of sequence data of growing genome projects reveal an unanticipated number of SAPLIP sequences in various organisms. Since the definite function of a SAPLIP is not deducib ...
ab initio
... •1978 Garnier improved the method by using statistically significant pair-wise interactions as a determinant of the statistical significance. This improved the success rate to 62% (page 447) •1993 Levin improved the prediction level by using multiple sequence alignments. The reasoning is as follows. ...
... •1978 Garnier improved the method by using statistically significant pair-wise interactions as a determinant of the statistical significance. This improved the success rate to 62% (page 447) •1993 Levin improved the prediction level by using multiple sequence alignments. The reasoning is as follows. ...
SIGNAL TRANSDUCTION PATHWAYS Outline
... Receptors found in either cytoplasm or nucleus Chemical messenger crosses target cell’s plasma membrane (hydrophobic or small enough; examples: steroid hormones; thyroid hormone; Nitric oxide) Chemical messenger binds to cytoplasmic receptor protein (as opposed to receptor protein in membrane). Horm ...
... Receptors found in either cytoplasm or nucleus Chemical messenger crosses target cell’s plasma membrane (hydrophobic or small enough; examples: steroid hormones; thyroid hormone; Nitric oxide) Chemical messenger binds to cytoplasmic receptor protein (as opposed to receptor protein in membrane). Horm ...
Bioknowlodgy worksheet 2.4
... 2.4.U7 Living organisms synthesize many different proteins with a wide range of functions. 13. Complete the table to describe each of different functions that proteins have in and outside of cells. ...
... 2.4.U7 Living organisms synthesize many different proteins with a wide range of functions. 13. Complete the table to describe each of different functions that proteins have in and outside of cells. ...
Olivoil Avenate Emulsifier - In
... DESCRIPTION: A new non-ethoxylated, vegetal derived emulsifier that combines the unique lipidic chains of olive oil with the characteristic affinity of hydrolyzed oat proteins toward the skin surface called Olivoyl Hydrolyzed Oat Protein, a lipo-protein with a fatty amide structure. Thanks to its sp ...
... DESCRIPTION: A new non-ethoxylated, vegetal derived emulsifier that combines the unique lipidic chains of olive oil with the characteristic affinity of hydrolyzed oat proteins toward the skin surface called Olivoyl Hydrolyzed Oat Protein, a lipo-protein with a fatty amide structure. Thanks to its sp ...
Gene Section SOCS6 (suppressor of cytokine signaling 6) in Oncology and Haematology
... SOCS6 has been shown to interact with the insulin receptor (INSR), INSR substrates IRS2 and IRS4, and negatively regulates insulin signaling (Krebs et al., 2002; Mooney et al., 2001). Although these findings suggest that SOCS6 might play a role in glucose metabolism, mice lacking SOCS6 gene did not ...
... SOCS6 has been shown to interact with the insulin receptor (INSR), INSR substrates IRS2 and IRS4, and negatively regulates insulin signaling (Krebs et al., 2002; Mooney et al., 2001). Although these findings suggest that SOCS6 might play a role in glucose metabolism, mice lacking SOCS6 gene did not ...
Research Express@NCKU
... resistant to viral infection. However, the role and the antiviral mechanisms of gMx protein in host cell defenses against nodavirus infection in grouper cells remains unclear. Our team focuses at the host-virus interaction study over six years. Piscine nodavirus, a member of the Betanodavirdae famil ...
... resistant to viral infection. However, the role and the antiviral mechanisms of gMx protein in host cell defenses against nodavirus infection in grouper cells remains unclear. Our team focuses at the host-virus interaction study over six years. Piscine nodavirus, a member of the Betanodavirdae famil ...
1st Prize: Alex Davison
... chaperones to interact with a newly-synthesised protein are ribosome-associated (RA) chaperones, which bind directly to the large ribosomal subunit. The concentration of RA chaperones in eukaryotic cytosol 'exceeds ribosome concentrations by a factor of roughly 2.5x'12. This suggests that on large o ...
... chaperones to interact with a newly-synthesised protein are ribosome-associated (RA) chaperones, which bind directly to the large ribosomal subunit. The concentration of RA chaperones in eukaryotic cytosol 'exceeds ribosome concentrations by a factor of roughly 2.5x'12. This suggests that on large o ...
NMR experiment-driven modeling of biological macromolecules
... data analysis, a relatively propitious protein candidate may be solved in a few weeks. Not surprisingly, around half of all protein structures solved in the Protein Data Bank using NMR have been thanks to automated, computational approaches. Here we will discuss emerging methods at the intersection ...
... data analysis, a relatively propitious protein candidate may be solved in a few weeks. Not surprisingly, around half of all protein structures solved in the Protein Data Bank using NMR have been thanks to automated, computational approaches. Here we will discuss emerging methods at the intersection ...
Protein Function - Gleason Chemistry
... Lock-and-key model devised by Emil Fischer in 1894. Substrate fits into corresponding rigid binding site on enzyme. Induced fit model: substrate fits into flexible pocket and imparts a conformational change in the enzyme. It is now observed that the conformational change is necessary in many protein ...
... Lock-and-key model devised by Emil Fischer in 1894. Substrate fits into corresponding rigid binding site on enzyme. Induced fit model: substrate fits into flexible pocket and imparts a conformational change in the enzyme. It is now observed that the conformational change is necessary in many protein ...
TD11 Identification of in vivo substrates of GroEL Nature 1999, 402
... Figure 1c shows after anti-GroEL IP now there are only 250-300 spots- 10% compared to control (indicates proteins that interact with GroEL), notice the predominance of larger proteins Figures 1e and f show how pI (isoelectric point) and MW compare to total proteins -pI distribution is about the same ...
... Figure 1c shows after anti-GroEL IP now there are only 250-300 spots- 10% compared to control (indicates proteins that interact with GroEL), notice the predominance of larger proteins Figures 1e and f show how pI (isoelectric point) and MW compare to total proteins -pI distribution is about the same ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.