Protein Expression and Purification Service Quotation Request Form
... Which species would you like to use? Mouse Rat Either one What application(s) you would use the antibody for? ELISA WB FC or FACS IF IP IHC ELISA Sandwich Other: If several applications are needed, please mention the preferred one below (if any): What kind of sample will the antibody be used on? Add ...
... Which species would you like to use? Mouse Rat Either one What application(s) you would use the antibody for? ELISA WB FC or FACS IF IP IHC ELISA Sandwich Other: If several applications are needed, please mention the preferred one below (if any): What kind of sample will the antibody be used on? Add ...
Cloning, expression, and characterization of Fe
... peroxisomes in cells. Although, this sequence directs other proteins to peroxisomes, it is yet to be determined whether this is a functional sequence or not. The conserved ARL (or SRL) sequence is not present in the prokaryotic FeSOD proteins, suggesting that it is not obligatory for the enzyme func ...
... peroxisomes in cells. Although, this sequence directs other proteins to peroxisomes, it is yet to be determined whether this is a functional sequence or not. The conserved ARL (or SRL) sequence is not present in the prokaryotic FeSOD proteins, suggesting that it is not obligatory for the enzyme func ...
SICB 2014 Annual Meeting Abstracts
... gene regulatory regions through a highly conserved DNA binding domain (DBD) composed of three C−terminal C2H2 zinc fingers (KLF−DBD). Members of the Klf gene family influence transcription via interactions with other transcription factors, cofactors, chromatin remodeling factors, and transcriptional ...
... gene regulatory regions through a highly conserved DNA binding domain (DBD) composed of three C−terminal C2H2 zinc fingers (KLF−DBD). Members of the Klf gene family influence transcription via interactions with other transcription factors, cofactors, chromatin remodeling factors, and transcriptional ...
Organic Compounds
... Carbohydrates are sugars, or long chains of sugars. An important role of carbohydrates is to store energy. Glucose ( Figure 1.1) is an important simple sugar molecule with the chemical formula C6 H12 O6 . Simple sugars are known as monosaccharides. Carbohydrates also include long chains of connected ...
... Carbohydrates are sugars, or long chains of sugars. An important role of carbohydrates is to store energy. Glucose ( Figure 1.1) is an important simple sugar molecule with the chemical formula C6 H12 O6 . Simple sugars are known as monosaccharides. Carbohydrates also include long chains of connected ...
Teaching the Concept of Protein Synthesis Rebecca
... • Abstract concept – may be hard for students to picture • Remembering that in RNA, Thymine is replaced with Uracil • Recognizing the matches between nucleotide bases and amino acids (i.e., using the genetic code table) ...
... • Abstract concept – may be hard for students to picture • Remembering that in RNA, Thymine is replaced with Uracil • Recognizing the matches between nucleotide bases and amino acids (i.e., using the genetic code table) ...
Interactions of Visinin-like Proteins with Phospho-inositides
... various signalling cascades in neuronal cells [1-3]. All members of the NCS protein family are EFhand proteins and share the typical feature of N-terminal myristoylation at the motif M-G-X 3-S, as well as the calcium-myristoyl switch. In response to elevated calcium levels, NCS proteins invoke the c ...
... various signalling cascades in neuronal cells [1-3]. All members of the NCS protein family are EFhand proteins and share the typical feature of N-terminal myristoylation at the motif M-G-X 3-S, as well as the calcium-myristoyl switch. In response to elevated calcium levels, NCS proteins invoke the c ...
Gene Section SS18 (synovial sarcoma translocation, chromosome 18) Atlas of Genetics and Cytogenetics
... 418 amino acids. 2 domains; the SHN domain (the SYT N-terminal homology domain) that is found in proteins from a wide variety of species ranging from plants to human, and the QPGY domain at the C-terminal part, rich in glutamine, proline, glycine and tyrosine. Four putative src-homology binding doma ...
... 418 amino acids. 2 domains; the SHN domain (the SYT N-terminal homology domain) that is found in proteins from a wide variety of species ranging from plants to human, and the QPGY domain at the C-terminal part, rich in glutamine, proline, glycine and tyrosine. Four putative src-homology binding doma ...
05lctout - Evergreen Archives
... 1. Once proteins are manufactured and processed through the ER and Golgi they must be sent to the appropriate location or organelle within the cell. 2. Studies involving lysosomal enzymes have revealed that these proteins are tagged with specific carbohydrate groups (mannose-6-phosphate) (Fig. 5.10) ...
... 1. Once proteins are manufactured and processed through the ER and Golgi they must be sent to the appropriate location or organelle within the cell. 2. Studies involving lysosomal enzymes have revealed that these proteins are tagged with specific carbohydrate groups (mannose-6-phosphate) (Fig. 5.10) ...
RNA seq Presentation
... • This quantity can be used for within sample analysis • Note: gene annotation and length come from an ‘exon model’ ...
... • This quantity can be used for within sample analysis • Note: gene annotation and length come from an ‘exon model’ ...
The Spurious Foundation of Genetic Engineering
... Alternative splicing can have an extraordinary impact on the gene/protein ratio. We now know that a single gene originally believed to encode a single protein that occurs in cells of the inner ear of chicks (and of humans) gives rise to 576 variant proteins, differing in their amino acid sequences. ...
... Alternative splicing can have an extraordinary impact on the gene/protein ratio. We now know that a single gene originally believed to encode a single protein that occurs in cells of the inner ear of chicks (and of humans) gives rise to 576 variant proteins, differing in their amino acid sequences. ...
Gene Section TFE3 (transcription factor E3) Atlas of Genetics and Cytogenetics
... Transcription factor; member of the basic helix-loophelix family (b-HLH) of transcription factors primarily found to bind to the immunoglobulin enhancer muE3 motif, Ig K enhancers and Ig H variable regions promotors; the helix-loop-helix-leucine zipper region is implicated in DNA binding and dimeriz ...
... Transcription factor; member of the basic helix-loophelix family (b-HLH) of transcription factors primarily found to bind to the immunoglobulin enhancer muE3 motif, Ig K enhancers and Ig H variable regions promotors; the helix-loop-helix-leucine zipper region is implicated in DNA binding and dimeriz ...
Modeling dynamics of cell-to-cell variability in TRAIL
... Noticing that g 1- g = 0 , g2 = g and that the probability distribution cannot charge states where x = -1 or y = -1, one gets: ...
... Noticing that g 1- g = 0 , g2 = g and that the probability distribution cannot charge states where x = -1 or y = -1, one gets: ...
From Gene to Protein—Transcription and Translation
... Draw a rectangle around the third codon in the messenger RNA. What is the anti-codon for that codon? The anti-codon for the third amino acid will be GAC Which amino acid will be the third amino acid in the hemoglobin protein? The third amino acid will be leucine. 12. Describe one similarity in the s ...
... Draw a rectangle around the third codon in the messenger RNA. What is the anti-codon for that codon? The anti-codon for the third amino acid will be GAC Which amino acid will be the third amino acid in the hemoglobin protein? The third amino acid will be leucine. 12. Describe one similarity in the s ...
Bacterial Shape: Concave Coiled Coils Curve
... have mreB homologs in their genomes. Interestingly, rhizobia and corynebacteria do not have MreB, but are still cylindrically shaped, possibly because they grow at their tips like filamentous fungi [5]. Their rod shape may depend on other proteins that are yet to be discovered. If MreB proteins are ...
... have mreB homologs in their genomes. Interestingly, rhizobia and corynebacteria do not have MreB, but are still cylindrically shaped, possibly because they grow at their tips like filamentous fungi [5]. Their rod shape may depend on other proteins that are yet to be discovered. If MreB proteins are ...
Malaria based proteomics of erythrocyte surface proteins
... New malaria drugs are expensive & limited in supply ...
... New malaria drugs are expensive & limited in supply ...
Are Protein Shakes A Good Idea?
... and what type of protein you eat. After a hard workout, even though you feel ready to quit, your body isn’t: The muscle-building process is about to begin. Your body is most primed to refuel starting about 45 minutes after you work out, up to about two hours. So it’s time to eat—ideally, carbs with ...
... and what type of protein you eat. After a hard workout, even though you feel ready to quit, your body isn’t: The muscle-building process is about to begin. Your body is most primed to refuel starting about 45 minutes after you work out, up to about two hours. So it’s time to eat—ideally, carbs with ...
Full Text
... biological system. This is particularly important because the rate of gene sequencing is increasing exponentially, and annotation of these novel sequences must be carried out in an automated manner. There are specific biological examples where only a small number of conserved amino acids are necessa ...
... biological system. This is particularly important because the rate of gene sequencing is increasing exponentially, and annotation of these novel sequences must be carried out in an automated manner. There are specific biological examples where only a small number of conserved amino acids are necessa ...
ab initio and Evidence-Based Gene Finding
... Many pseudogenes are mRNA’s that have retro-transposed back into the genome; many of these will appear as a single exon genes Increase vigilance for signs of a pseudogene when considering any single exon gene Alternatively, there may be missing exons ...
... Many pseudogenes are mRNA’s that have retro-transposed back into the genome; many of these will appear as a single exon genes Increase vigilance for signs of a pseudogene when considering any single exon gene Alternatively, there may be missing exons ...
Lecture #2 – Review of Protein Chemistry, Enzyme Specificity
... In this case, the R group looks nothing like a peptide, yet the enzyme is still active. The product here is an intermediate in the production of a pharmaceutical compound that was investigated for the treatment of benign prostate enlargement. This example raises two issues about enzyme specificity. ...
... In this case, the R group looks nothing like a peptide, yet the enzyme is still active. The product here is an intermediate in the production of a pharmaceutical compound that was investigated for the treatment of benign prostate enlargement. This example raises two issues about enzyme specificity. ...
Spectroscopy
... macromolecules, because of their enormous number of vibrational modes. • Biological macromolecules exhibit an intrinsic order of repeating units: • the peptide bond in the protein backbone, • the phosphate ester bond • IR spectra of biological macromolecules are simpler than at first expected • line ...
... macromolecules, because of their enormous number of vibrational modes. • Biological macromolecules exhibit an intrinsic order of repeating units: • the peptide bond in the protein backbone, • the phosphate ester bond • IR spectra of biological macromolecules are simpler than at first expected • line ...
Interactions between Human Two-pore Channels and Nonaspanin
... Two pore channels, a family consisting of TPC1, TPC2, TPC3, are cation-selective ion channels. Structurally, they contain two six transmembrane domains and form a dimer in the membrane (6). TPCs are found in plants and mammalian cells, and humans express two TPC isoforms TPC1 and TPC2, which locali ...
... Two pore channels, a family consisting of TPC1, TPC2, TPC3, are cation-selective ion channels. Structurally, they contain two six transmembrane domains and form a dimer in the membrane (6). TPCs are found in plants and mammalian cells, and humans express two TPC isoforms TPC1 and TPC2, which locali ...
PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS
... expression of up to 30% of the total cell protein. Despite these potential advantages, particular patterns of post-translational processing and expression must be empirically determined for each construct. Differences in proteins expressed by mammalian and baculovirus infected insect cells have been ...
... expression of up to 30% of the total cell protein. Despite these potential advantages, particular patterns of post-translational processing and expression must be empirically determined for each construct. Differences in proteins expressed by mammalian and baculovirus infected insect cells have been ...
Essentials of Glycobiology Lecture 6 (7) April 7th. (9) 1998 Ajit Varki
... Discovery of GPI-Anchors The C-terminus of Thy-1 glycoprotein found to have both fatty acids and ethanolamine. In 1981, Tony Holder and George Cross groups showed that soluble form of the variant surface glycoprotein (sVSG) of African trypanosomes contains an immunocrossreactive carbohydrate (C ...
... Discovery of GPI-Anchors The C-terminus of Thy-1 glycoprotein found to have both fatty acids and ethanolamine. In 1981, Tony Holder and George Cross groups showed that soluble form of the variant surface glycoprotein (sVSG) of African trypanosomes contains an immunocrossreactive carbohydrate (C ...
Sequence Alignment - UTK-EECS
... primary structure — sequence of amino acids in the protein secondary structure — polypeptide chains folding into regular structures (i.e., ...
... primary structure — sequence of amino acids in the protein secondary structure — polypeptide chains folding into regular structures (i.e., ...
PreAP Lesson Plan 8/25-8/29
... 8/27- 9A(R): SWBAT compare the 8/28 structure and functions of different types of biomolecules including carbohydrates, lipids, proteins, and nucleic acids. ...
... 8/27- 9A(R): SWBAT compare the 8/28 structure and functions of different types of biomolecules including carbohydrates, lipids, proteins, and nucleic acids. ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.