December 2009
... b. The lac operon would be induced in medium containing lactose but no glucose. c. The CAP binding protein would bind to the CAP binding site when glucose was absent. d. In glucose and lactose, β-galactosidase levels would be higher than normal. e. RNA polymerase would not be able to bind to the lac ...
... b. The lac operon would be induced in medium containing lactose but no glucose. c. The CAP binding protein would bind to the CAP binding site when glucose was absent. d. In glucose and lactose, β-galactosidase levels would be higher than normal. e. RNA polymerase would not be able to bind to the lac ...
Important advances in next generation genome editing
... One of these tricks they can do is to serve as a sort of stop sign for the cell. When the machinery that normally reads DNA arrives at the mutant HD gene, appropriately designed genome editing tools can call them off - telling them not to do their work at that precise gene. This results in no mutant ...
... One of these tricks they can do is to serve as a sort of stop sign for the cell. When the machinery that normally reads DNA arrives at the mutant HD gene, appropriately designed genome editing tools can call them off - telling them not to do their work at that precise gene. This results in no mutant ...
ppt - Computer Science & Engineering
... • Does this mean that there are no more than 25000 proteins in humans? • Alternate gene splicing provides the answer. CSCE 769 ...
... • Does this mean that there are no more than 25000 proteins in humans? • Alternate gene splicing provides the answer. CSCE 769 ...
video slide
... • Proto-oncogenes code for proteins involved in stimulating cell division (e.g. growth factors, growth factor receptors, cyclins) • Mutated proto-oncogenes that stimulate a cell to divide when it shouldn’t are called oncogenes (cancer-causing genes). • Tumor-suppressor genes code for proteins involv ...
... • Proto-oncogenes code for proteins involved in stimulating cell division (e.g. growth factors, growth factor receptors, cyclins) • Mutated proto-oncogenes that stimulate a cell to divide when it shouldn’t are called oncogenes (cancer-causing genes). • Tumor-suppressor genes code for proteins involv ...
Scoring Matrices: The Arrays Used to Find and Evaluate Protein Homologies
... • To Create Matrix: – For all blocks in all protein families, calculate the frequency that two related proteins have a specific pair of amino acids aligned within a block region. – Divide this by the frequency expected by chance. – This will produce a score for each pair of amino acids, which is rep ...
... • To Create Matrix: – For all blocks in all protein families, calculate the frequency that two related proteins have a specific pair of amino acids aligned within a block region. – Divide this by the frequency expected by chance. – This will produce a score for each pair of amino acids, which is rep ...
Scholarly Interest Report
... Beabout K, Hammerstrom TG, Wang TT, Bhatty M, Christie PJ, Saxer G, Shamoo Y. Rampant parasexuality evolves in a hospital pathogen during antibiotic selection. Molecular biology and evolution. 2015 32(10):2585-97 PMCID: PMC4683365 Davlieva M, Shi Y, Leonard PG, Johnson TA, Zianni MR, Arias CA, Ladb ...
... Beabout K, Hammerstrom TG, Wang TT, Bhatty M, Christie PJ, Saxer G, Shamoo Y. Rampant parasexuality evolves in a hospital pathogen during antibiotic selection. Molecular biology and evolution. 2015 32(10):2585-97 PMCID: PMC4683365 Davlieva M, Shi Y, Leonard PG, Johnson TA, Zianni MR, Arias CA, Ladb ...
SUPPLEMENTARY MATERIAL FOR:
... Table S1. The Affymetrix stem cell signature. Expression for 20,819 genes was detected from which 379 were found to be significantly enriched in the stem cells (see Materials and Methods) Table S2. The Agilent stem cell signature. Expression for 13,967 genes was detected from which 291 were found to ...
... Table S1. The Affymetrix stem cell signature. Expression for 20,819 genes was detected from which 379 were found to be significantly enriched in the stem cells (see Materials and Methods) Table S2. The Agilent stem cell signature. Expression for 13,967 genes was detected from which 291 were found to ...
Bioinformatics at IU
... functions. Proteomics research offers promises for: • novel drug discovery via the analysis of clinically relevant ...
... functions. Proteomics research offers promises for: • novel drug discovery via the analysis of clinically relevant ...
Lec-Functional Annotation and Functional Enrichment2010
... one or more ordered assemblies of molecular functions. • Examples of broad biological process terms are cellular physiological process or signal transduction. Examples of more specific terms are pyrimidine metabolic process or alpha-glucoside transport. • It can be difficult to distinguish between a ...
... one or more ordered assemblies of molecular functions. • Examples of broad biological process terms are cellular physiological process or signal transduction. Examples of more specific terms are pyrimidine metabolic process or alpha-glucoside transport. • It can be difficult to distinguish between a ...
Life Sciences 1a Practice Problems 6
... Practice Problems 6 1. HMG CoA Reductase is an enzyme involved in the biosynthesis of cholesterol. The protein and mRNA sequences were identified before the genomic sequence, and it was determined that the mRNA is 4471 nucleotides long, and encodes a protein of 888 amino acids. During the human geno ...
... Practice Problems 6 1. HMG CoA Reductase is an enzyme involved in the biosynthesis of cholesterol. The protein and mRNA sequences were identified before the genomic sequence, and it was determined that the mRNA is 4471 nucleotides long, and encodes a protein of 888 amino acids. During the human geno ...
The Bacterial Actin Cytoskeleton
... These two processes are carried out by redundant enzymes, some of which possess both catalytic activities combined in a single protein. Transpeptidation is very important because it is the target of the -lactam antibiotics. Indeed, the transpeptidases were first identified by their ability to bind ...
... These two processes are carried out by redundant enzymes, some of which possess both catalytic activities combined in a single protein. Transpeptidation is very important because it is the target of the -lactam antibiotics. Indeed, the transpeptidases were first identified by their ability to bind ...
Control of Gene Expression in Prokaryotes
... Houses usually have a light source in every room, but it would be a waste of energy to leave every light on all the time, so there are switches to turn off the lights in rooms that are not in use. Sometimes one switch controls several lights in the same room. Likewise, prokaryotic cells can turn gen ...
... Houses usually have a light source in every room, but it would be a waste of energy to leave every light on all the time, so there are switches to turn off the lights in rooms that are not in use. Sometimes one switch controls several lights in the same room. Likewise, prokaryotic cells can turn gen ...
ACT - Operon -S - saddlespace.org
... Houses usually have a light source in every room, but it would be a waste of energy to leave every light on all the time, so there are switches to turn off the lights in rooms that are not in use. Sometimes one switch controls several lights in the same room. Likewise, prokaryotic cells can turn gen ...
... Houses usually have a light source in every room, but it would be a waste of energy to leave every light on all the time, so there are switches to turn off the lights in rooms that are not in use. Sometimes one switch controls several lights in the same room. Likewise, prokaryotic cells can turn gen ...
Protein Structure - CS
... Protein Folds: sequential, spatial and topological arrangement of secondary structures ...
... Protein Folds: sequential, spatial and topological arrangement of secondary structures ...
BP DB (Recovered) - Base Pair Biotechnologies
... Base Pair Biotechnologies provides custom aptamer development services and catalog aptamers to academic, commercial, and government researchers for a variety of applications. To support their efforts we provide this series of aptamer best practices as a introduction to their use. Additional assistan ...
... Base Pair Biotechnologies provides custom aptamer development services and catalog aptamers to academic, commercial, and government researchers for a variety of applications. To support their efforts we provide this series of aptamer best practices as a introduction to their use. Additional assistan ...
Document
... insufficient dimers for the synthesis of new microtubules. C. causes deformation of microtubles. D. causes premature disassembly of microtubules. E. prevents the attachment of microtubules to chromosomes. ...
... insufficient dimers for the synthesis of new microtubules. C. causes deformation of microtubles. D. causes premature disassembly of microtubules. E. prevents the attachment of microtubules to chromosomes. ...
Expression of Semliki Forest Virus Proteins from Cloned
... Proteins with many different destinations are synthesized on polysomes bound to the endoplasmic reticulum (RER) (22). Some proteins become secreted from the cell, while others are routed to the cell surface or to various intracellular organelles. All of these proteins possess an N-terminal signal se ...
... Proteins with many different destinations are synthesized on polysomes bound to the endoplasmic reticulum (RER) (22). Some proteins become secreted from the cell, while others are routed to the cell surface or to various intracellular organelles. All of these proteins possess an N-terminal signal se ...
Leukaemia Section t(11;14)(p15;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... modulates transcription via intermolecular competitive binding between LIM domain and certain DNAbinding-transcription factors. The LDB1/NLI is a phosphoprotein and binds to LMO1 in its phophorylated state and essentially all the LMO1 and LDB1 protein in the T cell is part of the complexe. The stem ...
... modulates transcription via intermolecular competitive binding between LIM domain and certain DNAbinding-transcription factors. The LDB1/NLI is a phosphoprotein and binds to LMO1 in its phophorylated state and essentially all the LMO1 and LDB1 protein in the T cell is part of the complexe. The stem ...
Problem Set 5, 7.06, Spring 2003 1. In order to please your
... proteins, the nucleus, too. Everything was going fine, and you have tubes of protein extract from each of the organelles fresh out of the centrifuge and ready to be labelled (you're so good at the purification that you never label tubes until you are ready to put them in the freezer, but simply rely ...
... proteins, the nucleus, too. Everything was going fine, and you have tubes of protein extract from each of the organelles fresh out of the centrifuge and ready to be labelled (you're so good at the purification that you never label tubes until you are ready to put them in the freezer, but simply rely ...
Amino Acids
... Most are globular proteins that act as biological catalysts Holoenzymes consist of an apoenzyme (protein) and a cofactor (usually an ion) Enzymes are chemically specific ...
... Most are globular proteins that act as biological catalysts Holoenzymes consist of an apoenzyme (protein) and a cofactor (usually an ion) Enzymes are chemically specific ...
1. Metabolic regulation
... (carbohydrate response element binding protein). In hepatocites it is phosphorylated and it cannot go into the nucleus. When the protein is dephosphorylated by PP2A, it can enter the nucleus, where it undergoes a second dephosphorylation and can bind to Mix. The complex ChREBP-Mix binds the carbohyd ...
... (carbohydrate response element binding protein). In hepatocites it is phosphorylated and it cannot go into the nucleus. When the protein is dephosphorylated by PP2A, it can enter the nucleus, where it undergoes a second dephosphorylation and can bind to Mix. The complex ChREBP-Mix binds the carbohyd ...
Tutorial: Protein Synthesis - Integrated DNA Technologies
... The first feature of this generic gene that should be mentioned is that the part of the gene that codes for an amino acid sequence is not intact. The coding region is split into segments that code for protein, called exons, and segments that do not code for protein, called introns. The existence of ...
... The first feature of this generic gene that should be mentioned is that the part of the gene that codes for an amino acid sequence is not intact. The coding region is split into segments that code for protein, called exons, and segments that do not code for protein, called introns. The existence of ...
GFP Introduction Powerpoint Presentation - mvhs
... • Achieved through the use of selectable markers • Selectable markers = traits that help identify a cell with the plasmid in it (compared to one without it) • In our experiment, the bla gene will serve as the selectable marker Images taken without permission from http://www.antibioresistance.be/Gifs ...
... • Achieved through the use of selectable markers • Selectable markers = traits that help identify a cell with the plasmid in it (compared to one without it) • In our experiment, the bla gene will serve as the selectable marker Images taken without permission from http://www.antibioresistance.be/Gifs ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.