Important Factors Influencing Protein Solubility for 2-D - Bio-Rad
... solution can therefore have a strong influence on the proteins that show up in the 2-D pattern. Some proteins require ionic interactions for solubility, so salt solutions (for example, 150 mM NaCl) are often used for protein extraction. The use of salt requires caution because of its disruptive effe ...
... solution can therefore have a strong influence on the proteins that show up in the 2-D pattern. Some proteins require ionic interactions for solubility, so salt solutions (for example, 150 mM NaCl) are often used for protein extraction. The use of salt requires caution because of its disruptive effe ...
Lecture PPT - Carol Lee Lab
... • As of 2008, Cis-regulatory mutations represented ~22% of 331 identified genetic changes; although, the number of cis-regulatory changes published annually is rapidly increasing (there is a bias in the literature as more studies have examined amino acid changes) • Above the species level, cis-regul ...
... • As of 2008, Cis-regulatory mutations represented ~22% of 331 identified genetic changes; although, the number of cis-regulatory changes published annually is rapidly increasing (there is a bias in the literature as more studies have examined amino acid changes) • Above the species level, cis-regul ...
Protein Synthesis and Degradation
... All of the 16S, 23S and 5S rRNA chains, the A-, P- and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with protein ...
... All of the 16S, 23S and 5S rRNA chains, the A-, P- and E-site tRNAs, and most of the ribosomal proteins can be fitted to the electron density map. The core of the interface between the 30S small subunit and the 50S large subunit, where the tRNA substrates are bound, is dominated by RNA, with protein ...
Combinatorial library approaches for improving soluble protein
... colonies of transformants followed by detection of protein expression using an antibody against the fused hexahistidine tag. A few clones showed improved levels of expression, although they were mostly insoluble. While such sets of nested deletions are simple to produce and far cheaper than primer-d ...
... colonies of transformants followed by detection of protein expression using an antibody against the fused hexahistidine tag. A few clones showed improved levels of expression, although they were mostly insoluble. While such sets of nested deletions are simple to produce and far cheaper than primer-d ...
Here
... coordinated effort of a set of genes. Such activity is often carried out through the organization of the genome into regulatory modules. Modules are sets of co-regulated genes that share a common function. The identification of modules, their regulators, and the conditions under which regulation occ ...
... coordinated effort of a set of genes. Such activity is often carried out through the organization of the genome into regulatory modules. Modules are sets of co-regulated genes that share a common function. The identification of modules, their regulators, and the conditions under which regulation occ ...
Lecture 9 - Screening cDNA libraries (AMG text pp. 128-134)
... Biological processes require protein-protein interactions that can be defined by non-covalent molecular interactions. The idea of using a cloned protein to screen cDNA expression libraries for interacting proteins, follows from the work of Stan Fields and colleagues. The yeast two-hybrid strategy wa ...
... Biological processes require protein-protein interactions that can be defined by non-covalent molecular interactions. The idea of using a cloned protein to screen cDNA expression libraries for interacting proteins, follows from the work of Stan Fields and colleagues. The yeast two-hybrid strategy wa ...
Computational Biology
... Center for Protein Sequences) database (29). For this analysis, mitochondrial proteins were predicted with an accuracy of 70% as scored by the selfconsistency test. 10. Lecture WS 2003/04 ...
... Center for Protein Sequences) database (29). For this analysis, mitochondrial proteins were predicted with an accuracy of 70% as scored by the selfconsistency test. 10. Lecture WS 2003/04 ...
Membrane protein structure and assembly
... Heijne Nature Reviews Molecular Cell Biology 7, 909–918 (December 2006) | doi:10.1038/nrm2063 ...
... Heijne Nature Reviews Molecular Cell Biology 7, 909–918 (December 2006) | doi:10.1038/nrm2063 ...
File
... from one side of the membrane to the other side against concentration this process is known as active transport. ...
... from one side of the membrane to the other side against concentration this process is known as active transport. ...
INTRODUCTION TO MYCOLOGY
... Strain selection and further development are relatively straightforward, as these organisms are amendable to genetic modification. Process occupy little land area. Ex: Algal culture can be done in space that is normally unused and so there is no need to compete for land.. Production is independent o ...
... Strain selection and further development are relatively straightforward, as these organisms are amendable to genetic modification. Process occupy little land area. Ex: Algal culture can be done in space that is normally unused and so there is no need to compete for land.. Production is independent o ...
DNA and Protein Calculations
... 100pmol of 100kDa protein = 10µg 100pmol of 50kDa protein = 5µg 100pmol of 10kDa protein = 1µg 100pmol of 1kDa protein = 100ng ...
... 100pmol of 100kDa protein = 10µg 100pmol of 50kDa protein = 5µg 100pmol of 10kDa protein = 1µg 100pmol of 1kDa protein = 100ng ...
Supplementary figure legends
... YFP mRNA. The number of cells from whom the RNA was extracted was used for the normalization. Protein decay was estimated by monitoring CFP and YFP fluorescence by flow cytometry after different delays of treatment. Both mRNA and proteins decay following the respective treatments was fitted to an ex ...
... YFP mRNA. The number of cells from whom the RNA was extracted was used for the normalization. Protein decay was estimated by monitoring CFP and YFP fluorescence by flow cytometry after different delays of treatment. Both mRNA and proteins decay following the respective treatments was fitted to an ex ...
Anti-5HT1D Receptor antibody
... WB: 1/1000. Detects a band of approximately 42kDa kDa (predicted molecular weight: 45.2 kDa). ELISA: positive result against immunising peptide. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. ...
... WB: 1/1000. Detects a band of approximately 42kDa kDa (predicted molecular weight: 45.2 kDa). ELISA: positive result against immunising peptide. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. ...
PCDU Seminar Myriam Murillo 11 November 2015
... Binding of heterotetrameric adaptor protein 2 (AP-2) complex/clathrin to the PM. ...
... Binding of heterotetrameric adaptor protein 2 (AP-2) complex/clathrin to the PM. ...
NCBI genome database - Winona State University
... Acknowledgement: The presentation includes adaptations from NCBI’s ...
... Acknowledgement: The presentation includes adaptations from NCBI’s ...
2. Genetic methods
... If the goal of the expression is to produce large amount of functional protein, then it is necessary to consider whether it should be post-translationally modified or not. Example glycosylation might be needed for therapeutic proteins. This is important for the correct function of the protein and to ...
... If the goal of the expression is to produce large amount of functional protein, then it is necessary to consider whether it should be post-translationally modified or not. Example glycosylation might be needed for therapeutic proteins. This is important for the correct function of the protein and to ...
Interaction of a 14-3-3 protein with the plant
... poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictly regulated by the cell cycle, being expressed only during G2 and M phase (Pignocchi et al., 2009). 14-3-3 proteins, ident ...
... poles during mitosis and on the phragmoplast during cytokinesis indicates that EDE1 has a key role in microtubule function during mitosis. Also, its expression pattern is strictly regulated by the cell cycle, being expressed only during G2 and M phase (Pignocchi et al., 2009). 14-3-3 proteins, ident ...
EXPRESSING FOREIGN GENES IN MICE
... a viral gene in a mouse requires that the biologist remove the gene from the control of the viral promoter and fuse it to a promoter that is active in mouse cells. Brinster collaborated with Palmiter, who had been studying the promoter of the mouse metallothionein-1 (MT-1) gene. Palmiter fused the M ...
... a viral gene in a mouse requires that the biologist remove the gene from the control of the viral promoter and fuse it to a promoter that is active in mouse cells. Brinster collaborated with Palmiter, who had been studying the promoter of the mouse metallothionein-1 (MT-1) gene. Palmiter fused the M ...
msb201347-sup-0001 - Molecular Systems Biology
... disordered structure, we performed Wilcoxon-Mann-Whitney test between the two groups using the % disorder per protein as calculated from the human structure using disorder2 software(Ward et al, 2004). For KEN motif analysis, we calculated the number of KEN motifs in each protein sequence using an in ...
... disordered structure, we performed Wilcoxon-Mann-Whitney test between the two groups using the % disorder per protein as calculated from the human structure using disorder2 software(Ward et al, 2004). For KEN motif analysis, we calculated the number of KEN motifs in each protein sequence using an in ...
Function and Immunocytochemical Localization
... Since the identification of the first OBP, more than 400 OBP genes have been isolated and cloned. Among these genes, at least 150 are from lepidopteran species (Zhou 2010). In contrast to Lepidoptera, less work has focused on coleopteran OBPs. OBPs from some scarab beetles have been studied for 2 de ...
... Since the identification of the first OBP, more than 400 OBP genes have been isolated and cloned. Among these genes, at least 150 are from lepidopteran species (Zhou 2010). In contrast to Lepidoptera, less work has focused on coleopteran OBPs. OBPs from some scarab beetles have been studied for 2 de ...
chapt05_lecture_anim
... less fluid than unsaturated fatty acids • “Kinks” introduced by the double bonds keep them from packing tightly • Most membranes also contain sterols such as cholesterol, which can either increase or decrease membrane fluidity, depending on the temperature ...
... less fluid than unsaturated fatty acids • “Kinks” introduced by the double bonds keep them from packing tightly • Most membranes also contain sterols such as cholesterol, which can either increase or decrease membrane fluidity, depending on the temperature ...
Begrebet ”økologi” er i sig selv ikke ensbetydende med en bedre
... source is not defined as organic and is as well a limited resource. Therefore, it is necessary to replace as much as possible of the fish meal protein with competitive organic vegetable protein sources with high protein content as well as a relevant amino acid composition compared to that in fish me ...
... source is not defined as organic and is as well a limited resource. Therefore, it is necessary to replace as much as possible of the fish meal protein with competitive organic vegetable protein sources with high protein content as well as a relevant amino acid composition compared to that in fish me ...
the combination of two snps in the abcc2 gene, coding for multidrug
... Summary: Several findings indicate a role for ABC-type (ATP-binding cassette) drug efflux transport proteins in influencing the variability of clinical opioid effects. This phenomenon is attributed to variations in expression and activity secondary to genetic and environmental factors, leading to va ...
... Summary: Several findings indicate a role for ABC-type (ATP-binding cassette) drug efflux transport proteins in influencing the variability of clinical opioid effects. This phenomenon is attributed to variations in expression and activity secondary to genetic and environmental factors, leading to va ...
Wool Tech. and Sheep Breed. 50
... Major genes can lie in control regions and not affect the form of a protein but affect the rate of transcription and relative abundance. A desired gene may be present in a selected animal, but it may not always express the protein that leads to the desired metabolic and phenotypic effects. Each gene ...
... Major genes can lie in control regions and not affect the form of a protein but affect the rate of transcription and relative abundance. A desired gene may be present in a selected animal, but it may not always express the protein that leads to the desired metabolic and phenotypic effects. Each gene ...
senior biology - School of Medical Sciences
... Scientists have found more than 1000 different mutations of the CFTR gene; Some have little or no effect on CTFR function, while others cause cystic fibrosis on a spectrum that varies from mild to severe. Click on this link to view a database of all known mutations in the CFTR gene. http://www.gene ...
... Scientists have found more than 1000 different mutations of the CFTR gene; Some have little or no effect on CTFR function, while others cause cystic fibrosis on a spectrum that varies from mild to severe. Click on this link to view a database of all known mutations in the CFTR gene. http://www.gene ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.