RIBOSOME-INACTIVATING PROTEINS: A Plant Perspective
... only by tRNATrp from mammalian and avian cells and not by other tRNAs or tRNATrp from yeast (20–23). Given the variety of assays and assay conditions used for RIP analysis, some differences observed in previous studies may be explained by cofactor requirements. It is not known, however, whether the ...
... only by tRNATrp from mammalian and avian cells and not by other tRNAs or tRNATrp from yeast (20–23). Given the variety of assays and assay conditions used for RIP analysis, some differences observed in previous studies may be explained by cofactor requirements. It is not known, however, whether the ...
Automated extraction of mutation data from the literature: application
... The input data consists of abstracts and full text articles in plain text, Swiss-Prot entries (Boeckmann et al., 2003) for the target family, a dictionary of protein names and a list of mutation-like terms. Sequence information In the two examples we describe here, the Swiss-Prot entries are already ...
... The input data consists of abstracts and full text articles in plain text, Swiss-Prot entries (Boeckmann et al., 2003) for the target family, a dictionary of protein names and a list of mutation-like terms. Sequence information In the two examples we describe here, the Swiss-Prot entries are already ...
Two-step and one-step secretion mechanisms in Gram
... PapH in the growing pilus. This happens because PapH is unable to undergo DSE since it lacks a P5 pocket [27] (Figure 2C). No mechanism for type 1 pilus termination has been found yet as a PapH-like termination subunit is unknown in that pilus system. The usher assembly platform ...
... PapH in the growing pilus. This happens because PapH is unable to undergo DSE since it lacks a P5 pocket [27] (Figure 2C). No mechanism for type 1 pilus termination has been found yet as a PapH-like termination subunit is unknown in that pilus system. The usher assembly platform ...
The Molecular Basis of Diversity in the
... been supported by a number of physiological studies for the control of flowering time, indicating that the basis of daylength measurement is the interaction of an external light signal with a circadian rhythm (Thomas and Vince-Prue, 1997). In contrast, another model, called the internal coincidence ...
... been supported by a number of physiological studies for the control of flowering time, indicating that the basis of daylength measurement is the interaction of an external light signal with a circadian rhythm (Thomas and Vince-Prue, 1997). In contrast, another model, called the internal coincidence ...
THE ATOMIC NUCLEUS AND RADIOACTIVITY
... energy equivalence. Particles decay spontaneously only when their combined products have less mass after decay than before. The mass of a neutron is slightly greater than the total mass of a proton plus electron (and the antineutrino). So when a neutron decays, there is less mass after decay than be ...
... energy equivalence. Particles decay spontaneously only when their combined products have less mass after decay than before. The mass of a neutron is slightly greater than the total mass of a proton plus electron (and the antineutrino). So when a neutron decays, there is less mass after decay than be ...
Cloning and sequencing of a gene encoding acidophilic amylase
... Determination of the isoelectric point. Chromatofocusing was performed using Mono P 5/20 column according to the manufacturer's instructions (Pharmacia). a-Amylase activity and pH of each fraction was measured. Isoelectric focusing was done on 1 YO agarose gels containing pH 3-10 ampholyte (Pharmaci ...
... Determination of the isoelectric point. Chromatofocusing was performed using Mono P 5/20 column according to the manufacturer's instructions (Pharmacia). a-Amylase activity and pH of each fraction was measured. Isoelectric focusing was done on 1 YO agarose gels containing pH 3-10 ampholyte (Pharmaci ...
Whey Products - New Zealand Institute of Chemistry
... Most WPCs contain 5-7% milkfat in the dry powdered product. This fat originated in the milk and is not removed by the cream separators through which the whey passes before ultrafiltration. The most modern WPCs use either microfiltration (like ultrafiltration but using membranes with pores sized at a ...
... Most WPCs contain 5-7% milkfat in the dry powdered product. This fat originated in the milk and is not removed by the cream separators through which the whey passes before ultrafiltration. The most modern WPCs use either microfiltration (like ultrafiltration but using membranes with pores sized at a ...
Metabolic rate depression in animals
... considered herein derives from laboratory experimentation using animals induced to enter a hypometabolic state by the acute imposition of stress (e.g. exposure to a nitrogen gas atmosphere for studies of anoxia tolerance in marine snails). The use of controlled experimental conditions allows the ...
... considered herein derives from laboratory experimentation using animals induced to enter a hypometabolic state by the acute imposition of stress (e.g. exposure to a nitrogen gas atmosphere for studies of anoxia tolerance in marine snails). The use of controlled experimental conditions allows the ...
Polyubiquitination in diseases: implications in skeletal muscle
... MyoD. RFP and Pax7 synergistically reduced the protein amounts of MyoD but not the mRNA. RFPinduced reduction of MyoD protein was also blocked by MG132, a proteasome inhibitor. The Pax7induced reduction MyoD was attenuated by RFP siRNA and by MG132. RFPR, an RFP construct that lacks the RING domain ...
... MyoD. RFP and Pax7 synergistically reduced the protein amounts of MyoD but not the mRNA. RFPinduced reduction of MyoD protein was also blocked by MG132, a proteasome inhibitor. The Pax7induced reduction MyoD was attenuated by RFP siRNA and by MG132. RFPR, an RFP construct that lacks the RING domain ...
Acyl Carrier Protein (ACP) lmport into Chloroplasts Does not
... We have recently developed an assay for organelle-free processing of the precursor of light-harvesting chlorophyll a/b-binding protein in which the transit peptide is removed (Lamppa and Abad, 1987; Abad, Clark, and Lamppa, 1989). In addition, the crude soluble extract processes the small subunit of ...
... We have recently developed an assay for organelle-free processing of the precursor of light-harvesting chlorophyll a/b-binding protein in which the transit peptide is removed (Lamppa and Abad, 1987; Abad, Clark, and Lamppa, 1989). In addition, the crude soluble extract processes the small subunit of ...
Molecular Microbiology
... observable growth defect on soybean cultivar OAC Bayfield, a significant growth advantage is conferred by HopZ1b to the growth of a non-pathogenic P. syringae strain on soybean (Zhou et al., 2009). Functional redundancy is very common for T3SEs in P. syringae (Alfano and Collmer, 2004; Kvitko et al. ...
... observable growth defect on soybean cultivar OAC Bayfield, a significant growth advantage is conferred by HopZ1b to the growth of a non-pathogenic P. syringae strain on soybean (Zhou et al., 2009). Functional redundancy is very common for T3SEs in P. syringae (Alfano and Collmer, 2004; Kvitko et al. ...
Biology Item and Scoring Sampler 2011
... The Biology Keystone Exam includes questions that require students to select the best answer from four possible answer options. Students read each question and record their answers in the space provided. The correct answer for each multiple-choice question is worth one point. The Biology Keystone Ex ...
... The Biology Keystone Exam includes questions that require students to select the best answer from four possible answer options. Students read each question and record their answers in the space provided. The correct answer for each multiple-choice question is worth one point. The Biology Keystone Ex ...
Biology Item and Scoring Sampler 2011
... The Biology Keystone Exam includes questions that require students to select the best answer from four possible answer options. Students read each question and record their answers in the space provided. The correct answer for each multiple-choice question is worth one point. The Biology Keystone Ex ...
... The Biology Keystone Exam includes questions that require students to select the best answer from four possible answer options. Students read each question and record their answers in the space provided. The correct answer for each multiple-choice question is worth one point. The Biology Keystone Ex ...
Isolation and Expression Pattern of Human Unc-33
... other neural cells (Fishman et al., 1990, 1991; Greenlee et al., 1993). The cultures were then fixed in 4% paraformaldehyde and analyzed. They were first immunostained using the Rip monoclonal antibody and microphotographed using a 40⫻ objective (Z eiss). Processes were quantified on the photographs ...
... other neural cells (Fishman et al., 1990, 1991; Greenlee et al., 1993). The cultures were then fixed in 4% paraformaldehyde and analyzed. They were first immunostained using the Rip monoclonal antibody and microphotographed using a 40⫻ objective (Z eiss). Processes were quantified on the photographs ...
Facing extremes: archaeal surface-layer (glyco)proteins
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
Presentation - people.vcu.edu
... • Do myelination and oligodendrocytes play a role in the Rett syndrome phenotype? • Does MeCP2 specifically affect MBP and MAG? ...
... • Do myelination and oligodendrocytes play a role in the Rett syndrome phenotype? • Does MeCP2 specifically affect MBP and MAG? ...
Nutrition, Anabolism, and the Wound Healing Process: An Overview
... Adequate protein intake to meet the demands ...
... Adequate protein intake to meet the demands ...
Gene Section ARNT (aryl hydrocarbon receptor nuclear translocator) Atlas of Genetics and Cytogenetics
... protein due to omission of exon 5 have been noted. A transcript of about 1300 nucleotides is observed in some breast cancers and may be due to an alternative splicing event leading to elimination of the 3' end of the ...
... protein due to omission of exon 5 have been noted. A transcript of about 1300 nucleotides is observed in some breast cancers and may be due to an alternative splicing event leading to elimination of the 3' end of the ...
Chapter 6 Identifying and Measuring Transmembrane Helix–Helix
... kinases, to just name a few. Recently, the importance of the TM regions has emerged: TM domains, and more specifically, helix–helix interactions, not only are major determinants of the assembly and stability of the native protein structure but also play significant roles in membrane insertion and fo ...
... kinases, to just name a few. Recently, the importance of the TM regions has emerged: TM domains, and more specifically, helix–helix interactions, not only are major determinants of the assembly and stability of the native protein structure but also play significant roles in membrane insertion and fo ...
1 Causality, Transfer Entropy and Allosteric
... this sense all proteins may be regarded as intrinsically allosteric in nature [3]. This new view freed the understanding of allostery from the limited picture of discrete two state transitions and opened a broader vista in terms of entropy transfer in proteins. The idea of transfer entropy, recently ...
... this sense all proteins may be regarded as intrinsically allosteric in nature [3]. This new view freed the understanding of allostery from the limited picture of discrete two state transitions and opened a broader vista in terms of entropy transfer in proteins. The idea of transfer entropy, recently ...
Disulfide formation in plant storage vacuoles permits assembly
... During endosperm development in the castor oil seed of Ricinus communis, lipids and proteins accumulate in oil bodies and protein storage vacuoles (PSV), respectively. The most abundant of the storage proteins, which provide an early source of amino acids to fuel post-germinative growth, are the 11S ...
... During endosperm development in the castor oil seed of Ricinus communis, lipids and proteins accumulate in oil bodies and protein storage vacuoles (PSV), respectively. The most abundant of the storage proteins, which provide an early source of amino acids to fuel post-germinative growth, are the 11S ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.