F9550 - Datasheet - Sigma
... Stop reactions by the addition 5 µl stop solution. Boil for 5 min. at 95 °C. Load 4 µl sample on the denaturing gel. Note: wash the wells before loading. Run the mini gel at 200V with circulating cold water (~10°C) to reduce heating until the stain front reaches 1-2 cm of the bottom of the gel (brom ...
... Stop reactions by the addition 5 µl stop solution. Boil for 5 min. at 95 °C. Load 4 µl sample on the denaturing gel. Note: wash the wells before loading. Run the mini gel at 200V with circulating cold water (~10°C) to reduce heating until the stain front reaches 1-2 cm of the bottom of the gel (brom ...
Nucleotide Metabolism -Biosynthesis- Dr. Sooad Al
... Denovo Purine Nucleotide Biosynthesis The two parent purine nucleotides of nucleic acids are adenosine 5monophosphate (AMP) and guanosine 5-monophosphate (GMP). The origin of the carbon and nitrogen atoms of the purine ring system, as determined by John Buchanan using isotopic tracer experiment ...
... Denovo Purine Nucleotide Biosynthesis The two parent purine nucleotides of nucleic acids are adenosine 5monophosphate (AMP) and guanosine 5-monophosphate (GMP). The origin of the carbon and nitrogen atoms of the purine ring system, as determined by John Buchanan using isotopic tracer experiment ...
A novel dipeptidomimetic containing a cyclic threonine.
... properties when compared to the natural analogs. For instance, these compounds contain an additional a-substituent which result in a tertiary a-carbon like in 1-amino-2-hydroxycyclohexane carboxylic acid derivatives.17–20 Apart from this, Thr has been used for the assembly of several cyclic systems ...
... properties when compared to the natural analogs. For instance, these compounds contain an additional a-substituent which result in a tertiary a-carbon like in 1-amino-2-hydroxycyclohexane carboxylic acid derivatives.17–20 Apart from this, Thr has been used for the assembly of several cyclic systems ...
Vitamins Clinical relevance: homocystinuria: B6 and/or B12 and/or
... o flavins: Zn++ via (non-covalent, electrostatic) Metabolite Cofactors o produced by metabolic pathways and used by other enzymes to carry out key rxns: ATP ...
... o flavins: Zn++ via (non-covalent, electrostatic) Metabolite Cofactors o produced by metabolic pathways and used by other enzymes to carry out key rxns: ATP ...
C485 Exam I
... a) 8 pts How many enzymatic activities are required for glycogen synthesis? Briefly explain each one. 4 UDP glucose pyrophosphorylase takes UTP and G1-P to UDP glucose and pyrophosphate Glycogenin makes primers by taking UDP glucose and adding it to a tyrosine residue. It then elongates by three mor ...
... a) 8 pts How many enzymatic activities are required for glycogen synthesis? Briefly explain each one. 4 UDP glucose pyrophosphorylase takes UTP and G1-P to UDP glucose and pyrophosphate Glycogenin makes primers by taking UDP glucose and adding it to a tyrosine residue. It then elongates by three mor ...
A Supramolecular Peptide Synthesizer
... high yields. SPPS follows a stepwise protocol to add amino acids one at a time to a growing peptide chain. Importantly, each amino acid building block has to be activated and orthogonally protected at its N-terminus and functional side chains. The selective removal of the N-terminal protecting group ...
... high yields. SPPS follows a stepwise protocol to add amino acids one at a time to a growing peptide chain. Importantly, each amino acid building block has to be activated and orthogonally protected at its N-terminus and functional side chains. The selective removal of the N-terminal protecting group ...
review-examIII-2011
... drive glycogen synthesis. the interconversion between glycogen and glucose lphosphate. the production of lactate from glucose in peripheral tissues with the resynthesis of glucose from lactate in liver. the synthesis of alanine from pyruvate in skeletal muscle and the synthesis of pyruvate from alan ...
... drive glycogen synthesis. the interconversion between glycogen and glucose lphosphate. the production of lactate from glucose in peripheral tissues with the resynthesis of glucose from lactate in liver. the synthesis of alanine from pyruvate in skeletal muscle and the synthesis of pyruvate from alan ...
Inhibitors
... Specificity IC 50 for other related molecules Use lowest dose possible Verify it does not affect other pathways ...
... Specificity IC 50 for other related molecules Use lowest dose possible Verify it does not affect other pathways ...
Protein mteabolism
... Conversion of aromatic acids into specialized products 1- Phenylalanine amino acid Essential amino acid Converted into tyrosine (major pathway ) ...
... Conversion of aromatic acids into specialized products 1- Phenylalanine amino acid Essential amino acid Converted into tyrosine (major pathway ) ...
fatty acid synthesis
... synthesis. Phosphorylation of ACC, for example a result of activation of PKA by stress or exercise switches on fatty acid oxidation (via phosphorylation and inhibition of ACC-2 resulting in decreased malonyl CoA levels) while switching off fatty acid synthesis (via phosphorylation and inhibition of ...
... synthesis. Phosphorylation of ACC, for example a result of activation of PKA by stress or exercise switches on fatty acid oxidation (via phosphorylation and inhibition of ACC-2 resulting in decreased malonyl CoA levels) while switching off fatty acid synthesis (via phosphorylation and inhibition of ...
Biomolecule 20 Questions
... 8) Which of the following statements about the monomers and polymers found in living organisms is false? A) The monomers used to make polymers are essentially universal. B) Cells typically make all of their macromolecules from a set of 40–50 common monomers and a few other ingredients that are rare. ...
... 8) Which of the following statements about the monomers and polymers found in living organisms is false? A) The monomers used to make polymers are essentially universal. B) Cells typically make all of their macromolecules from a set of 40–50 common monomers and a few other ingredients that are rare. ...
Principles of Biochemistry. 4th Edition International Student Version Brochure
... 24 DNA Structure and Interactions with Proteins 821 25 DNA Synthesis and Repair 867 26 RNA Metabolism 919 27 The Genetic Code and Translation 962 28 Gene Expression in Prokaryotes and Eukaryotes 1013 Solutions to Odd–Numbered Problems SP–1 Glossary G–1 Index I–1 ...
... 24 DNA Structure and Interactions with Proteins 821 25 DNA Synthesis and Repair 867 26 RNA Metabolism 919 27 The Genetic Code and Translation 962 28 Gene Expression in Prokaryotes and Eukaryotes 1013 Solutions to Odd–Numbered Problems SP–1 Glossary G–1 Index I–1 ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... Discuss the salient features of Crick and Watson model for the structure of DNA. ...
... Discuss the salient features of Crick and Watson model for the structure of DNA. ...
Pentose phosphate pathway = PPP Pentose phosphate cycle
... fatty acid (and further TAG) synthesis in adipocytes 4.) some molecules that induce reactions of biotransformation, induce PPP dehydrogenase enzymes as well, to produce NADPH for biotransformation ...
... fatty acid (and further TAG) synthesis in adipocytes 4.) some molecules that induce reactions of biotransformation, induce PPP dehydrogenase enzymes as well, to produce NADPH for biotransformation ...
Synthesis of RNA - Stamm revision
... site analogs can be introduced, as well as fluorophores (e.g., fluorescein and its ...
... site analogs can be introduced, as well as fluorophores (e.g., fluorescein and its ...
26.7 LABORATORY SYNTHESIS OF PEPTIDES
... The properties of a protein depend primarily on its three-dimensional structure. The sequence of amino acids in the polypeptide chain is termed its primary structure. Its secondary structure is the shape of the backbone polypeptide chain. Remember that each amide group is planar, but the chain can h ...
... The properties of a protein depend primarily on its three-dimensional structure. The sequence of amino acids in the polypeptide chain is termed its primary structure. Its secondary structure is the shape of the backbone polypeptide chain. Remember that each amide group is planar, but the chain can h ...
26.3 Synthesis of Amino Acids
... Peptide synthesis requires that different amide bonds must be formed in a desired sequence The growing chain is protected at the carboxyl terminal and added amino acids are N-protected After peptide bond formation, N-protection is removed ...
... Peptide synthesis requires that different amide bonds must be formed in a desired sequence The growing chain is protected at the carboxyl terminal and added amino acids are N-protected After peptide bond formation, N-protection is removed ...
Discovery-4 - Peptide Machines, Inc.
... different scales and protocols. Non-stop operation with reaction vessels that can function independently, and the ability to add and remove sequences without interrupting instrument operations add to the ease of use. ...
... different scales and protocols. Non-stop operation with reaction vessels that can function independently, and the ability to add and remove sequences without interrupting instrument operations add to the ease of use. ...
Multiple Choice Questions- Chemistry and Metabolism of nucleotides
... increased levels of serum uric acid. These symptoms are characteristic of Lesch Nyhan syndrome, which is due to defectivea) Salvage pathway for pyrimidine biosynthesis b) Denovo synthesis of pyrimidines c) Xanthine oxidase d) HGPRT (Hypoxanthine Guanine Phospho Ribosyl Transferase) e) Formyl transfe ...
... increased levels of serum uric acid. These symptoms are characteristic of Lesch Nyhan syndrome, which is due to defectivea) Salvage pathway for pyrimidine biosynthesis b) Denovo synthesis of pyrimidines c) Xanthine oxidase d) HGPRT (Hypoxanthine Guanine Phospho Ribosyl Transferase) e) Formyl transfe ...
IOSR Journal of Applied Chemistry (IOSR-JAC)
... 2278-5736. Volume 7, Issue 10 Ver. I. (Oct. 2014), PP 01-05. In continuation of this research another novel βLactam derivative amino acid was synthesized. NEPA-NCA (III) i.e. [(S)-ethyl-2-((S)-4-methyl-2,5-dioxooxazolidin-3-yl)-4-phenyl butanoate], is an important side chain component used in the sy ...
... 2278-5736. Volume 7, Issue 10 Ver. I. (Oct. 2014), PP 01-05. In continuation of this research another novel βLactam derivative amino acid was synthesized. NEPA-NCA (III) i.e. [(S)-ethyl-2-((S)-4-methyl-2,5-dioxooxazolidin-3-yl)-4-phenyl butanoate], is an important side chain component used in the sy ...
Sample exam
... 14. Which molecules drawn above would you attribute the property of amphipathic. 15. Which processes below consume more energy than they produce? (consume ATP, NADPH etc ) Gluconeogenesis Glycolysis Citric acid cycle Cholesterol synthesis Pentose phosphate pathway Fatty acid oxidation Fatty acid bio ...
... 14. Which molecules drawn above would you attribute the property of amphipathic. 15. Which processes below consume more energy than they produce? (consume ATP, NADPH etc ) Gluconeogenesis Glycolysis Citric acid cycle Cholesterol synthesis Pentose phosphate pathway Fatty acid oxidation Fatty acid bio ...
Pa I I, hl. L. Blasticidin-S: on... Cycloheximide has been used widely as ...
... with blosticidin-S giver significant inhibition of incorporation into protein. incubation with 5Opg/ml blarticidin-S monohydrochloride giver almost complete inhibition of incorporation. is ...
... with blosticidin-S giver significant inhibition of incorporation into protein. incubation with 5Opg/ml blarticidin-S monohydrochloride giver almost complete inhibition of incorporation. is ...
Pentose Phosphate Pathway (aka Hexose monophosphate shunt)
... • G6PD is the most regulated enzyme: inhibition by NADPH, expression is dependent on insulin thus it is only expressed at high glucose concentration • Since the non-oxidative pathway is reversible, the direction is dependent on the need of the cell for ATP / acetyl CoA (energy / fatty acid synthesis ...
... • G6PD is the most regulated enzyme: inhibition by NADPH, expression is dependent on insulin thus it is only expressed at high glucose concentration • Since the non-oxidative pathway is reversible, the direction is dependent on the need of the cell for ATP / acetyl CoA (energy / fatty acid synthesis ...
Document
... • Look at the slides that follow containing the list of reactions on which to focus. • Re-read the indicated pages where each reaction is described in Creighton. Look at related power point slides and examples discussed in class. • Put together a summary sheet with each reaction in as much detail as ...
... • Look at the slides that follow containing the list of reactions on which to focus. • Re-read the indicated pages where each reaction is described in Creighton. Look at related power point slides and examples discussed in class. • Put together a summary sheet with each reaction in as much detail as ...
Oligonucleotide synthesis
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not suffer from this limitation, although it is, most often, carried out in the opposite, 3' to 5' direction. Currently, the process is implemented as solid-phase synthesis using phosphoramidite method and phosphoramidite building blocks derived from protected 2'-deoxynucleosides (dA, dC, dG, and T), ribonucleosides (A, C, G, and U), or chemically modified nucleosides, e.g. LNA, BNA.To obtain the desired oligonucleotide, the building blocks are sequentially coupled to the growing oligonucleotide chain in the order required by the sequence of the product (see Synthetic cycle below). The process has been fully automated since the late 1970s. Upon the completion of the chain assembly, the product is released from the solid phase to solution, deprotected, and collected. The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15–25 bases in length.Oligonucleotides find a variety of applications in molecular biology and medicine. They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes.