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Secretory Protein mRNA Finds Another Way Out
Secretory Protein mRNA Finds Another Way Out

... no longer command the spotlight, especially compared with the smoking hot gene-regulating microRNAs or the Piwiinteracting RNAs, with their steamy role in spermatogenesis. As the intermediary between the gene blueprint and the protein end product, mRNAs certainly play a role of fundamental importanc ...
a more thorough description of current interests.
a more thorough description of current interests.

... catalytic capabilities that combine features of enzymes and traditional transition-metal catalysts. One crucial feature of biological catalysis that we have sought to emulate is the ability to override inherent chemical reactivity, performing site-specific chemistry in a functional-group-rich enviro ...
Chemistry of Life
Chemistry of Life

... – Progesterone: allows women to be pregnant ...
poster - Olson Lab
poster - Olson Lab

... Brugia as an outgroup. Gene models from Hymenolepis are indicated by the prefix HmN, and are followed by the accession information for the corresponding transcript data, as indicated by the prefix Aby or Contig. ...
Lipids - Cloudfront.net
Lipids - Cloudfront.net

... The “tail” end is scared of water and is given the name HYDROPHOBIC You will see why this is so important when we talk about ...
Diversity of proteins
Diversity of proteins

... •partial or complete unfolding of native conformation ...
Poster
Poster

... researchers used a technique called NMR (Nuclear Magnetic Resonance) that allows structure determination of proteins in an aqueous solution, as opposed to a solid crystal. As a result, the researchers obtained 30 distinct possible structures of A-beta. The image below depicts 15 of these structures ...
Curriculum Vitae
Curriculum Vitae

... molecular mechanisms of hormone signal transduction and transcriptional regulatory complexes by adapting diverse molecular, biochemical, cellular and genetic techniques. Anthocyanins, a class of plant secondary metabolites distributed ubiquitously in the plant kingdom, endow plants with a colorful w ...
Functional and Structural Characterization of a Prokaryotic Peptide
Functional and Structural Characterization of a Prokaryotic Peptide

... H⫹/peptide symport is measured. Previous comparisons of the characteristics of rheogenic transporters employing this new cellfree electrophysiological technique with findings from patch clamp studies revealed a very good correlation in all features (21). Blue Native Gel Electrophoresis—Linear 5–12% ...
d) Structural Proteins
d) Structural Proteins

... Silk: a stack of anti-parallel beta-sheets (PDB 1slk) within a sheet: covalent bonding and hydrogen bond, between sheets: Van der Waals force F-actin: helical assemblies of actin and actin-associated proteins  muscle contraction, cytoskeleton ...
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose

... upon a viscous medium, the gel, where an electric field forces the DNA to migrate toward the positive potential, the anode, due to the net negative charge of the phosphate backbone of the DNA chain. The separation of the DNA fragments from your PCR reaction is accomplished by exploiting the mobiliti ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe

... • add 4µl DIG high prime labeling mix; mix briefly and tap spin • incubate overnight at 37°C. • stop reaction by adding 2µl 0.2M EDTA (pH 8) and heat inactivate at 65°C 10min • boil probe 10-20min before using • used probe can be stored at -20°C in Hybridization Buffer and used repetedly ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe

... • add 4µl DIG high prime labeling mix; mix briefly and tap spin • incubate overnight at 37°C. • stop reaction by adding 2µl 0.2M EDTA (pH 8) and heat inactivate at 65°C 10min • boil probe 10-20min before using • used probe can be stored at -20°C in Hybridization Buffer and used repetedly TRANSFER OF ...
Power Point 1 - G. Holmes Braddock
Power Point 1 - G. Holmes Braddock

... formed from various amino acids. By convention, a chain under 40 residues is often identified as a peptide, rather than a protein. To be able to perform their biological function, proteins fold into one or more specific spatial conformations, driven by a number of noncovalent interactions such as hy ...
Protein Denaturation Studies Using the Pyris 6 DSC
Protein Denaturation Studies Using the Pyris 6 DSC

... instruments to study the thermal properties of proteins in aqueous solution is becoming increasingly more important. In an aqueous solution, proteins have specialized threedimensional structures that allows them to support specific biological functions. When heat is applied to the protein, this shap ...
Structures of
Structures of

... 4.  Protein Stability 5.  Protein Folding ...
PHS 398 (Rev. 9/04), Biographical Sketch Format Page
PHS 398 (Rev. 9/04), Biographical Sketch Format Page

... YEAR(s) ...
ANALYTICAL BIOCHEMISTRY
ANALYTICAL BIOCHEMISTRY

... K , Ca Redox, including O2-responsive systems. Titrations, poteniometric, amperometric, and conductimetric. The use of such methods in dynamic situations such as enzyme rate determination and for semi-automatic analysis should be understood. Ion selective electrodes Biosensors Chromatographic and El ...
Station 1: Carbon Compounds
Station 1: Carbon Compounds

... Organic chemistry is the study of all compounds that contain bonds between carbon atoms. Carbon compounds are also called organic compounds. Many of the molecules in living things are so large that they are known as macromolecules. Macromolecules are formed in a process called polymerization. Smalle ...
Supplementary Methods (a) Chemically
Supplementary Methods (a) Chemically

... basis. To calculate the relative abundance for each protein sample type (per gel lane), the total ...
Gene Section RHOBTB1 (Rho-related BTB domain containing 1) in Oncology and Haematology
Gene Section RHOBTB1 (Rho-related BTB domain containing 1) in Oncology and Haematology

... heterozygosity at the RHOBTB1 locus was identified in 12 of 52 tumour samples. RHOBTB1 showed decreased expression levels in 37% of 46 tumour samples and all informative low-expression samples displayed loss of heterozygosity. Three silent nucleotide changes were found in a mutation analysis (Beder ...
Nutritional biochemistry
Nutritional biochemistry

... 2-Classification on the basis of structure. a-simple protein: they consist of a.a only & not join to any other compound. They are classified according to their shape into:  Fibrous protein: has long , extended, sheet like shape , high mechanical strength, low solubility and digestability e.g collag ...
Maxi Care Plus TechSheet.pub
Maxi Care Plus TechSheet.pub

... Large Breeds: Feed 2.3 quarts or fill 2 quart bottle to rim and feed twice daily. In extremely cold weather it may be beneficial to feed another feeding of calf milk replacer in the middle of the day. Add 6 ounces of milk replacer powder to 1 quart of 110-120° F. water, mix and feed to 1 calf at mid ...
Detection of Cellular Response to an in vitro Challenge with
Detection of Cellular Response to an in vitro Challenge with

... complex search strategy (Figure 4), breaks the PTM search strategy into multiple nodes, where small groups of PTMs, likely to occur on the same peptide, are searched in each node. This allows for higher-confidence assignments due to the reduced size of each database, albeit at an increased search co ...
Lecture 6 and 7
Lecture 6 and 7

... Internal standard to make sure that abundance is normalised and variation Is due to biological variation rather than gel-to-gel variation Oct 2011 SDMBT ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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