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Topic 2: Molecular biology (21 hours)
Topic 2: Molecular biology (21 hours)

... carbohydrates, lipids, proteins and nucleic acids. • Aim 7: ICT can be used for molecular visualization of • Metabolism is the web of all the enzyme-catalysed carbohydrates, lipids and proteins in this sub-topic and reactions in a cell or organism. in 2.3 and 2.4. • Anabolism is the synthesis of com ...
Proteomics_12-8
Proteomics_12-8

... Nesvizhskii (2010) J. Proteomics, 73:20922123. ...
PROTEIN CHEMISTRY
PROTEIN CHEMISTRY

... of protein) interacts with itself to form a stable three-dimensional structure during production of the protein within the cell. The folding of proteins thus facilitates the production of discrete functional entities, including enzymes and structural proteins, which allow the various processes assoc ...
AB057PSI_AOAPO_KBALAMURUGAN_22092016
AB057PSI_AOAPO_KBALAMURUGAN_22092016

... Host-pathogen interaction studies at the protein level attract greater interest by providing profound knowledge on different dimensions of interface between host and pathogen. The alarming increase of epidemic infections by several drug-resistant bacteria renowned the necessity of insightful knowled ...
Graph preprocessing
Graph preprocessing

... original as well as the transformed networks  Nabieva et al.’s FunctionalFlow algorithm o neighborhood-based algorithm inspired by Schwikowski et al.’s function prediction algorithm o The predictions from both these algorithms are evaluated within a five-fold cross-validation setup by computing the ...
Buffer Solutions in Ocular Irrigation
Buffer Solutions in Ocular Irrigation

... eye will be diluted and/or washed out by the large ...
Note
Note

... i. the greater the concentration of H+ ions, the more acidic the solution b. bases are substances that accept protons or H+ ions from acids i. often have OH- ions ii. the greater the concentration of OH- ions, the more basic the solution c. strong acids or bases dissociate more completely in solutio ...
Cells Lecture V
Cells Lecture V

... phosphate head and two fattyacid tails is a monomer of the polymer known as the cell membrane The phosphate head of the phospholipid attracts water because it is polar while the fatty-acid tails repel it as it passes through the membrane ...
Unit 10 web
Unit 10 web

... • Both have Fe II containing heme unit in each chain that binds O2. ...
Document
Document

...  = partial specific volume of the solute (units: cm3/g) One rough estimation of the partial specific volume of a protein, which may be used if the sequence of the protein is not known, is: average partial specific volume of proteins = 0.725 cm^3/g Because the average of experimentally determined p ...
Small G-protein
Small G-protein

... COPII vesicle formation is mediated by a monomeric GTPase. A GEF in the donor membrane interacts with the GTPase, Sar1, causing GDP/GTP exchange. Sar1-GTP extends a fatty acid tail that inserts into the membrane. COPII assembles on the Sar1 to form a vesicle. COPI vesicle formation involves a protei ...
A. thaliana genotyping with a CAPS marker for a pks3
A. thaliana genotyping with a CAPS marker for a pks3

... 3 fragments: 29 bp, 182 bp, and 307 bp. The pks3-7 mutant sequence is missing an MboI restriction site, and digestion of the mutant 518 bp fragment yielded 2 fragments: 211 bp and 307 bp. Digested samples were analyzed using the QIAxcel capillary electrophoresis system with the QIAxcel DNA Screening ...
protein - mustafaaltinisik.org.uk
protein - mustafaaltinisik.org.uk

... molecular weight of hemoglobin. 1933 Tiselius introduced electrophoresis for separating proteins in solution. 1942 Martin and Synge developed chromatography, a technique now widely used to separate proteins. 1951 Pauling and Corey proposed the structure of a helical conformation of a chain of L-amin ...
Chapter 3 Problem Set
Chapter 3 Problem Set

... migrate towards the (1) cathode; (2) cathode; (3) not migrate; and (4) anode when placed in an electric field. ...
Biotechnology Laboratory (Kallas)
Biotechnology Laboratory (Kallas)

... media containing light (N14) and heavy (N15) isotopes of nitrogen, harvesting and mixing these cultures, breaking and fractionating the cells, digesting the mixed proteins with trypsin, and subjecting the protein fragments (peptides) to liquid chromatography and tandem mass spectrometry (LCMS/MS). F ...
protein-complex_cros..
protein-complex_cros..

... – PTM of both complex and target substrates that alter quaternary structure – Compartmentation (e.g. COP9 signalasome; TIR1p) – Subunit stoichiometry (may be variable and dynamic) – Supra-molecular complex formation ...
Chapter 3 - Slothnet
Chapter 3 - Slothnet

... from hydrogen bonding between N–H groups on one amino acid and C=O groups on another. • β pleated sheet—two or more polypeptide chains are aligned; hydrogen bonds form between the chains. ...
(Submitted) Genetic Synthesis of Periodic Protein Materials M. J.
(Submitted) Genetic Synthesis of Periodic Protein Materials M. J.

... folding of mRNA which could impair translation and composition of an early mRNA region known to influence activity); and, 3) the strategy for cloning. Thus, the final cloning strategy is based on: 1) how the synthetic DNA will be joined enzymatically to the cloning and expression vectors; 2) analysi ...
没有幻灯片标题
没有幻灯片标题

... removed, the enzymatic activity was slowly regained (enzymatic activity was fully regained under stable conditions, with existence of trace amount of bmercaptoethanol. 11.2.5 All the physical and chemical properties of the refolded enzyme were virtually identical with those of the native enzyme. 11. ...
Confocal Laser Scanning Microscopy
Confocal Laser Scanning Microscopy

... successive stages of development, stained with actinspecific rhodamine-labeled phalloidin. Whole embryos (AE) and the first 2-3 legs (F-J) are shown. Such preparations were used to study neurogenesis in myriapods as compared with neurogenesis in insects and chelicerates. From Dove & ...
Practical molecular biology
Practical molecular biology

... Type III enzymes recognize two separate non-palindromic sequences that are inversely oriented. They cut DNA about 20-30 base pairs after the recognition site. ...
Proteins
Proteins

... • Those growing or ill require larger amounts of protein. • Calculation of N balance is used to determine protein equilibrium. • Excess protein cannot be stored and is converted into glucose or fat for later use. • Athletes who are highly trained for endurance activities may need to exceed the RDA f ...
Overview of Protein Structure • The three
Overview of Protein Structure • The three

... interior is one of the principle forces stabilizing the native structure of proteins. However, this places the polar nitrogen and oxygen atoms of the protein main chain in a very hydrophobic environment in which their hydrogen bonding potential must be satisfied. This problem has been solved by prot ...
Table S6: Domains present in the primary network generated from
Table S6: Domains present in the primary network generated from

... This presumed domain is functionally uncharacterised. This uncharacterised family of proteins are principally found in cyanobacteria. This domain is found in a set of hypothetical bacterial proteins. Its exact function has not, as yet, been defined. This family of proteins are functionally uncharact ...
GPSDB: a new database for synonyms expan
GPSDB: a new database for synonyms expan

... database, specific fields were extracted (official name, symbol name, synonyms, database cross-reference links, species name, entry ID, etc.). In order to retrieve a complete list of synonyms for a given gene/protein, all entries from the databases above relating to a same entity were merged. The id ...
< 1 ... 89 90 91 92 93 94 95 96 97 ... 184 >

QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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