SecStAnT: secondary structure analysis tool for data selection
... Biomolecules coordinates are stored in a format that is a widely used, internationally referred representation for macromolecular data, including experimental and structural information. These, however, are integrated within the coordinates file, and not of immediate use to the aim of building, e.g. ...
... Biomolecules coordinates are stored in a format that is a widely used, internationally referred representation for macromolecular data, including experimental and structural information. These, however, are integrated within the coordinates file, and not of immediate use to the aim of building, e.g. ...
PCR-based gene synthesis to produce recombinant proteins for
... Structural genomics consortia have exploited gene synthesis technology to produce difficult recombinant proteins for structural analysis [1]. In most cases, if not all, synthetic genes are fabricated commercially for convenience and time efficiency. Presently, the affordability of synthetic oligonuc ...
... Structural genomics consortia have exploited gene synthesis technology to produce difficult recombinant proteins for structural analysis [1]. In most cases, if not all, synthetic genes are fabricated commercially for convenience and time efficiency. Presently, the affordability of synthetic oligonuc ...
The Case Against a Darwinian Origin of Protein Folds
... manipulate? Although we are some way from a complete answer to this, several aspects of the relationship between enzyme structures and their functions provide at least partial answers. On the most basic level, it has become clear that protein chains have to be of a certain length in order to fold in ...
... manipulate? Although we are some way from a complete answer to this, several aspects of the relationship between enzyme structures and their functions provide at least partial answers. On the most basic level, it has become clear that protein chains have to be of a certain length in order to fold in ...
Projection Structure of a Plant Vacuole Membrane Aquaporin by
... Purification of a -TIP a-TIP was puri®ed from bean seeds (Figure 1), where it comprises approximately 2 % of the total extractable protein in cotyledons (Johnson et al., 1989). From 100 g of dry mass of seed, 4-8 mg of a-TIP was routinely obtained. Mature, ungerminated seeds were used so that the pu ...
... Purification of a -TIP a-TIP was puri®ed from bean seeds (Figure 1), where it comprises approximately 2 % of the total extractable protein in cotyledons (Johnson et al., 1989). From 100 g of dry mass of seed, 4-8 mg of a-TIP was routinely obtained. Mature, ungerminated seeds were used so that the pu ...
THE EFFECTS OF SALTS ON THE STABILITY OF THE COLLAGEN
... salt concentration with T m for calcium chloride is about five times that for sodium chloride. At an anion concentration of o· 4M the respective lowerings of T m at neutral pH are about 3 and 1 degG whereas at pH 3·0 the corresponding values are 7·0 and 6·6 degG. Thus at pH 3·0 not only are the magn ...
... salt concentration with T m for calcium chloride is about five times that for sodium chloride. At an anion concentration of o· 4M the respective lowerings of T m at neutral pH are about 3 and 1 degG whereas at pH 3·0 the corresponding values are 7·0 and 6·6 degG. Thus at pH 3·0 not only are the magn ...
Replacement Matrices for Transmembrane Proteins
... Since 1985, GenBank, the sequence database maintained by the National Center for Biotechnology Information, has doubled in size every 18 months [4]. At this pace, novel sequences cannot be characterized by experimental methods alone. The emergence of phylogenetics software in the past decades allows ...
... Since 1985, GenBank, the sequence database maintained by the National Center for Biotechnology Information, has doubled in size every 18 months [4]. At this pace, novel sequences cannot be characterized by experimental methods alone. The emergence of phylogenetics software in the past decades allows ...
AZT resistance of simian foamy virus reverse transcriptase is based
... HIV-2 RT controls the incorporation of the inhibitor nucleotide AZTTP (11), whereas for HIV-1 RT excision of the incorporated AZTMP has been recognized as the mechanism of resistance (8–10). Thus, we first analyzed the polymerization behavior of the enzymes in the presence of AZTTP to check for incor ...
... HIV-2 RT controls the incorporation of the inhibitor nucleotide AZTTP (11), whereas for HIV-1 RT excision of the incorporated AZTMP has been recognized as the mechanism of resistance (8–10). Thus, we first analyzed the polymerization behavior of the enzymes in the presence of AZTTP to check for incor ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
... having between nine to about forty amino acids are simply named as peptides. The name polypeptide is generally used for chain lengths of over forty amino acids. As we shall see later in this chapter, very high levels of complexities in the structure of polypeptide chains are achieved when they bend ...
... having between nine to about forty amino acids are simply named as peptides. The name polypeptide is generally used for chain lengths of over forty amino acids. As we shall see later in this chapter, very high levels of complexities in the structure of polypeptide chains are achieved when they bend ...
Enzymes - Westgate Mennonite Collegiate
... - high temps may denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions) ...
... - high temps may denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions) ...
Enzymes - Westgate Mennonite Collegiate
... - high temps may denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions) ...
... - high temps may denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions) ...
Dissociation
... — Although no compound is ever totally insoluble, compounds of very low solubility can be considered insoluble for most practical purposes — There is no easy method to predict whether a compound made up of a certain combination of ions will be soluble when put into water — However, general solubilit ...
... — Although no compound is ever totally insoluble, compounds of very low solubility can be considered insoluble for most practical purposes — There is no easy method to predict whether a compound made up of a certain combination of ions will be soluble when put into water — However, general solubilit ...
מצגת של PowerPoint
... A main achievement of this study was to expose student teachers to the unusual teaching strategy of questioning which enables junior high school students to overcome the understanding gap of abstract Micro level biological processes. It is clear that, given the rudimentary knowledge of the junior hi ...
... A main achievement of this study was to expose student teachers to the unusual teaching strategy of questioning which enables junior high school students to overcome the understanding gap of abstract Micro level biological processes. It is clear that, given the rudimentary knowledge of the junior hi ...
Protocol: Cloning of oligos for sgRNA (CRISPR) or
... 1.5 µL of forward oligo (100uM) 1.5 µL of reverse oligo (100uM) 5 µL of 10x NEB buffer 3.1 42 µL ddH20 ---------------------50ul total ...
... 1.5 µL of forward oligo (100uM) 1.5 µL of reverse oligo (100uM) 5 µL of 10x NEB buffer 3.1 42 µL ddH20 ---------------------50ul total ...
Amino Acids 2 Questions
... 2. Gel‐filtration chromatography separates a mixture of proteins on the basis of: A) size B) charge C) affinity for ligands in the column matrix D) density 3. What is the purpose of treating a protein with 2‐mercaptoethanol? A) To hydrolyze the protein into its amino acids. B) To derivatize a ...
... 2. Gel‐filtration chromatography separates a mixture of proteins on the basis of: A) size B) charge C) affinity for ligands in the column matrix D) density 3. What is the purpose of treating a protein with 2‐mercaptoethanol? A) To hydrolyze the protein into its amino acids. B) To derivatize a ...
Importance of main-chain hydrophobic free energy to the stability of
... obvious reasons to say that one would be more stable than others. Even examination of the amino acids and comparison of structural features of thermophiles with mesophilies cannot bring satisfactory explanation for the thermal stability of such proteins. In order to bring out the hidden information ...
... obvious reasons to say that one would be more stable than others. Even examination of the amino acids and comparison of structural features of thermophiles with mesophilies cannot bring satisfactory explanation for the thermal stability of such proteins. In order to bring out the hidden information ...
Aquaporin JcPIP2 is Involved in Drought Responses in Jatropha
... GaoYou CSC63, whereas there was no significant change in seedlings of YanBian S1. Compared with YanBian S1, GaoYou CSC63 also showed higher root hydraulic conductivity and lower decreasing trend in the seedlings under water deficit. These results indicated that JcPIP2 probably played a role in droug ...
... GaoYou CSC63, whereas there was no significant change in seedlings of YanBian S1. Compared with YanBian S1, GaoYou CSC63 also showed higher root hydraulic conductivity and lower decreasing trend in the seedlings under water deficit. These results indicated that JcPIP2 probably played a role in droug ...
Chapter 2 powerpoint file
... Temperature- each protein has a adequate temperature for it’s function. Outside of the range it may be denatured of inactivated. ...
... Temperature- each protein has a adequate temperature for it’s function. Outside of the range it may be denatured of inactivated. ...
The cyanide hydratase from Neurospora crassa forms a helix
... cyanide hydratases (CHT) (O’Reilly and Turner 2003). In an accompanying paper (Basile et al. 2008), we describe the characterization of new members of the cyanide hydratase family and their comparison to enzymes previously characterized. Engineering improved enzymes for such direct applications is o ...
... cyanide hydratases (CHT) (O’Reilly and Turner 2003). In an accompanying paper (Basile et al. 2008), we describe the characterization of new members of the cyanide hydratase family and their comparison to enzymes previously characterized. Engineering improved enzymes for such direct applications is o ...
characteristics and stabilization of dnaase
... One object of the present investigation was to study this phenomenon further. A major difficulty in the study of cell-free protein synthesis in h’. coli systems has been the necessity for preparing fresh enzyme extracts for each experiment. Techniques have not been available for stabilization and st ...
... One object of the present investigation was to study this phenomenon further. A major difficulty in the study of cell-free protein synthesis in h’. coli systems has been the necessity for preparing fresh enzyme extracts for each experiment. Techniques have not been available for stabilization and st ...
Summary and conclusions - Food Standards Australia New Zealand
... Figure 4: Schematic representation of the junction sequences detected in MON87751 ........17 Figure 5: Breeding path for generating segregation data for MON87751 ..............................19 Figure 6: Schematic representation of the origin of Cry1A.105 protein domains ...................23 Figur ...
... Figure 4: Schematic representation of the junction sequences detected in MON87751 ........17 Figure 5: Breeding path for generating segregation data for MON87751 ..............................19 Figure 6: Schematic representation of the origin of Cry1A.105 protein domains ...................23 Figur ...
Data Analysis Tools & Techniques - II
... Microarray data format • Unlike sequence and structural data, there is no international convention for the representation of data from microarray experiments • This is due to the wide variation in experimental design, assay platforms and methodologies • Recently, an initiative to develop a common l ...
... Microarray data format • Unlike sequence and structural data, there is no international convention for the representation of data from microarray experiments • This is due to the wide variation in experimental design, assay platforms and methodologies • Recently, an initiative to develop a common l ...
Genetically engineered gold-binding polypeptides
... Abstract—The biological control of inorganic crystal formation, morphology, and assembly is of interest to biologists and biotechnologists studying hard tissue growth and regeneration, as well as to materials scientists using biomimetic approaches for the control of inorganic material fabrication an ...
... Abstract—The biological control of inorganic crystal formation, morphology, and assembly is of interest to biologists and biotechnologists studying hard tissue growth and regeneration, as well as to materials scientists using biomimetic approaches for the control of inorganic material fabrication an ...
Enzymes: “Helper” Protein molecules
... other molecules very little enzyme needed to help in many reactions ...
... other molecules very little enzyme needed to help in many reactions ...
Targeting of Proteins to the Outer Envelope Membrane Uses a
... the cDNA insert was used to synthesize nucleic acid probes to screen another Xgt11 cDNA library. Among the positive clones, A14kom was chosen for further study because it contained the largest insert. The cDNA insert of X14kom was subcloned into the expression vector pSP65 (Promega), resulting in th ...
... the cDNA insert was used to synthesize nucleic acid probes to screen another Xgt11 cDNA library. Among the positive clones, A14kom was chosen for further study because it contained the largest insert. The cDNA insert of X14kom was subcloned into the expression vector pSP65 (Promega), resulting in th ...
Principles of immunological Techniques
... To produce the antiserum, the antigen is injected subcutaneously or intraperitonelly into and experimental animal, usually the rabbit, together with an adjuvant to stimulate the immune system. Large molecules with MW over 10000 are naturally antigenic and cause the production of antibodies. Small mo ...
... To produce the antiserum, the antigen is injected subcutaneously or intraperitonelly into and experimental animal, usually the rabbit, together with an adjuvant to stimulate the immune system. Large molecules with MW over 10000 are naturally antigenic and cause the production of antibodies. Small mo ...