protein
... higher orders of structure are determined principally by noncovalent forces such as hydrogen bonds and ionic, van der Waals, and hydrophobic interactions. • It is important to emphasize that all the information necessary for a protein molecule to achieve its intricate architecture is contained withi ...
... higher orders of structure are determined principally by noncovalent forces such as hydrogen bonds and ionic, van der Waals, and hydrophobic interactions. • It is important to emphasize that all the information necessary for a protein molecule to achieve its intricate architecture is contained withi ...
Conformational flexibility may explain multiple cellular roles of PEST
... protein interaction. In our earlier study, we found that these motifs contribute largely to eukaryotic protein disorder. This observation led us to evaluate their conformational variability in the nonredundant Protein Data Bank (PDB) structures. For this purpose, crystallographic temperature factors ...
... protein interaction. In our earlier study, we found that these motifs contribute largely to eukaryotic protein disorder. This observation led us to evaluate their conformational variability in the nonredundant Protein Data Bank (PDB) structures. For this purpose, crystallographic temperature factors ...
Cdiff_expression_supmat_BiolInv.
... encodes a a novel transcriptional repressor harboring two double-stranded RNAbinding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. Protein exhibits innate phosphatase activit ...
... encodes a a novel transcriptional repressor harboring two double-stranded RNAbinding domains and a region homologous to the catalytic domain of RNA polymerase II C-terminal domain phosphatases found in yeast and in animals that regulate gene transcription. Protein exhibits innate phosphatase activit ...
FTIR Analysis of Protein Structure
... each. A logarithmic scale is used for time scale because the exchange is an exponentially decaying process. From this experiment we made peak assignments for both the hydrogenated and deuterated forms of each of the major elements of secondary structure. As shown in Figure 11, the assigned peaks mir ...
... each. A logarithmic scale is used for time scale because the exchange is an exponentially decaying process. From this experiment we made peak assignments for both the hydrogenated and deuterated forms of each of the major elements of secondary structure. As shown in Figure 11, the assigned peaks mir ...
Cell and Molecular Biology
... N-linked glycosylation of ER proteins. Most of the glycosylation associated with proteins outside the cell begins in the ER. A large preformed oligosaccharide is transferred from a lipid called dolichol to the side-chain of asparagine. Because the covalent linkage is to the nitrogen of the asparagi ...
... N-linked glycosylation of ER proteins. Most of the glycosylation associated with proteins outside the cell begins in the ER. A large preformed oligosaccharide is transferred from a lipid called dolichol to the side-chain of asparagine. Because the covalent linkage is to the nitrogen of the asparagi ...
Protein Structure - Research Centers
... Sodium dodecyl sulfate: strong detergent, applied to protein Charged species binds quantitatively Denatures protein ...
... Sodium dodecyl sulfate: strong detergent, applied to protein Charged species binds quantitatively Denatures protein ...
Genes affecting starch biosynthesis exert pleiotropic effects on the
... of total protein (compare Figs 2 and 3). Mutants with legumin contents that appeared to be lower than wildtype (r, rb, rrb, rug5, rug3 sim1 and rug3 sim32) tended to have total protein contents higher than wild-type. Those mutants with similar pro®les to wild-type (rug4 and lam), and possibly higher ...
... of total protein (compare Figs 2 and 3). Mutants with legumin contents that appeared to be lower than wildtype (r, rb, rrb, rug5, rug3 sim1 and rug3 sim32) tended to have total protein contents higher than wild-type. Those mutants with similar pro®les to wild-type (rug4 and lam), and possibly higher ...
The Serum Proteins of the Rat During Development
... albumin component was detected in the present work. The relative concentration of the pre-albumin listed by Gurvich & Karsaevskaya (1956) ranges from 16-00 to 21 -0 per cent., whereas the largest average value measured in the present work was 11-3±2-9 per cent. It is likely, therefore, that the pre- ...
... albumin component was detected in the present work. The relative concentration of the pre-albumin listed by Gurvich & Karsaevskaya (1956) ranges from 16-00 to 21 -0 per cent., whereas the largest average value measured in the present work was 11-3±2-9 per cent. It is likely, therefore, that the pre- ...
Flow Cytometry Protein A and Protein G Antibody Binding Beads
... for each fluorochrome-labeled antibody as follows: 1. Place one drop (~50µL, ~100,000 beads) of Antibody Binding Beads into a test tube. Add the fluorochrome-conjugated antibody that is being used for cell labeling. You may use the amount suggested by the antibody supplier for cell labeling, or mor ...
... for each fluorochrome-labeled antibody as follows: 1. Place one drop (~50µL, ~100,000 beads) of Antibody Binding Beads into a test tube. Add the fluorochrome-conjugated antibody that is being used for cell labeling. You may use the amount suggested by the antibody supplier for cell labeling, or mor ...
Molecular Imprinting of Maltose Binding Protein
... and covered by a mica wafer. The imprinted polymers (MIPs) were prepared in the same way by adding MBP labeled with Cy3 (MBP-Cy3) (1 mg mL1 in Tris buffer) to the precursor solution to achieve a final concentration of 0.5 mg mL1. Polymerization was conducted at 35 °C for 3 h. After polymerization, t ...
... and covered by a mica wafer. The imprinted polymers (MIPs) were prepared in the same way by adding MBP labeled with Cy3 (MBP-Cy3) (1 mg mL1 in Tris buffer) to the precursor solution to achieve a final concentration of 0.5 mg mL1. Polymerization was conducted at 35 °C for 3 h. After polymerization, t ...
SC.912.L.18.11 Explain the role of enzymes as catalysts that lower
... substance on which an enzyme acts during a chemical reaction is called a substrate. Enzymes act only on specific substrates. An enzyme’s shape determines its activity. Typically, an enzyme is a large protein with one or more deep folds on its surface. These folds form pockets called active sites. As ...
... substance on which an enzyme acts during a chemical reaction is called a substrate. Enzymes act only on specific substrates. An enzyme’s shape determines its activity. Typically, an enzyme is a large protein with one or more deep folds on its surface. These folds form pockets called active sites. As ...
Trends in Plant Science
... domains (e.g. BLOCK [6]). More recently, several programs (Table 1) have been released that are designed to identify conserved sequence segments associated with a particular function. Studies based on these programs suggest that many proteins in various organisms contain at least one such unit or do ...
... domains (e.g. BLOCK [6]). More recently, several programs (Table 1) have been released that are designed to identify conserved sequence segments associated with a particular function. Studies based on these programs suggest that many proteins in various organisms contain at least one such unit or do ...
Protein Catabolism
... The biosynthesis of proteins requires a continuous source of amino acids. Amino acids also provide cells with a source of nitrogen for the synthesis of nitrogen containing biomolecules such as nucleotides. Amino acids are generated by the digestion of proteins in the intestine or by the degradation ...
... The biosynthesis of proteins requires a continuous source of amino acids. Amino acids also provide cells with a source of nitrogen for the synthesis of nitrogen containing biomolecules such as nucleotides. Amino acids are generated by the digestion of proteins in the intestine or by the degradation ...
Protein and vegetarian diets - Medical Journal of Australia
... (expressed as a percentage of total energy intake) that would allow for an adequate intake of all the other nutrients. The NRV document notes that while, on average, only 10% of energy need be consumed as protein to meet the physiological need for protein, this level is insufficient to allow for est ...
... (expressed as a percentage of total energy intake) that would allow for an adequate intake of all the other nutrients. The NRV document notes that while, on average, only 10% of energy need be consumed as protein to meet the physiological need for protein, this level is insufficient to allow for est ...
Macromolecules
... denaturation. Denaturing agents disrupt stabilizing factors. Agents that disrupt hydrogen bonding: Heat -- thermal agitation (vibration, etc.) -- will denature proteins or nucleic acids. Heat denaturation of DNA is called melting because the transition from native to denatured state occurs over a na ...
... denaturation. Denaturing agents disrupt stabilizing factors. Agents that disrupt hydrogen bonding: Heat -- thermal agitation (vibration, etc.) -- will denature proteins or nucleic acids. Heat denaturation of DNA is called melting because the transition from native to denatured state occurs over a na ...
Essential Cell Biology (3rd ed.)
... two regions of a polypeptide chain together tightly. The stability of each folded shape will therefore be affected by the combined strength of large numbers of noncovalent bonds (figure 4–4). A fourth weak force, hydrophobic interaction, also plays a central role in determining the shape of a protei ...
... two regions of a polypeptide chain together tightly. The stability of each folded shape will therefore be affected by the combined strength of large numbers of noncovalent bonds (figure 4–4). A fourth weak force, hydrophobic interaction, also plays a central role in determining the shape of a protei ...
Identification of a Protein with Antioxidant Activity that is Important for
... shelf life by minimising the oxygen content and hence ROS in the process of brewing using varieties of antioxidant compounds such as polyphenols, sulphites, sulphur dioxide and vitamins. However, little attention has been paid to one of the major component in beers–beer proteins. Many major antioxid ...
... shelf life by minimising the oxygen content and hence ROS in the process of brewing using varieties of antioxidant compounds such as polyphenols, sulphites, sulphur dioxide and vitamins. However, little attention has been paid to one of the major component in beers–beer proteins. Many major antioxid ...
Molecular biology technique (I) Southern/Northern
... • Hybridization-process of forming a doublestranded DNA molecule between a singlestranded DNA probe and a single-stranded target patient DNA. ...
... • Hybridization-process of forming a doublestranded DNA molecule between a singlestranded DNA probe and a single-stranded target patient DNA. ...
Interpro - European Bioinformatics Institute
... – what are these proteins; to what family do they belong? – what is their function; how can we explain this in structural terms? ...
... – what are these proteins; to what family do they belong? – what is their function; how can we explain this in structural terms? ...
manual Monarch DNA Gel Extraction Kit T1020S T1020L
... concentrated high-quality, double-stranded DNA from agarose gels. This method employs a bind/wash/elute workflow with minimal incubation and spin times, resulting in purification in less than 15 minutes. The Monarch Gel Dissolving Buffer is used to dissolve the agarose gel slice and ensure the sampl ...
... concentrated high-quality, double-stranded DNA from agarose gels. This method employs a bind/wash/elute workflow with minimal incubation and spin times, resulting in purification in less than 15 minutes. The Monarch Gel Dissolving Buffer is used to dissolve the agarose gel slice and ensure the sampl ...
Protein aggregation and amyloid fibril formation prediction software
... aggregation propensities of the 20 natural amino acids (a3v). Next, calculations are based on the sliding-window averaging technique: a sliding window of a given length is chosen and the program calculates the average of a3v values over the sliding window and assigns it to the central residue of the ...
... aggregation propensities of the 20 natural amino acids (a3v). Next, calculations are based on the sliding-window averaging technique: a sliding window of a given length is chosen and the program calculates the average of a3v values over the sliding window and assigns it to the central residue of the ...
PCR-based cloning from plasmids Entered by Karin Holmberg
... • Directional cloning with two restriction enzymes is preferred over non-directional cloning with one enzyme • Make sure that the restriction-enzyme buffer is appropriate for the double digest (check the NEB table). • The new 1.1, 2.1, 3.1, 4.1, and CutSmart buffers that come with NEB restriction en ...
... • Directional cloning with two restriction enzymes is preferred over non-directional cloning with one enzyme • Make sure that the restriction-enzyme buffer is appropriate for the double digest (check the NEB table). • The new 1.1, 2.1, 3.1, 4.1, and CutSmart buffers that come with NEB restriction en ...
New Title - Gravette School District
... include galactose, which is a component of milk, and fructose, which is found in many fruits. The large macromolecules formed from monosaccharides are known as polysaccharides. Many animals store excess sugar in a polysaccharide called glycogen, or animal starch. When the level of glucose in your bl ...
... include galactose, which is a component of milk, and fructose, which is found in many fruits. The large macromolecules formed from monosaccharides are known as polysaccharides. Many animals store excess sugar in a polysaccharide called glycogen, or animal starch. When the level of glucose in your bl ...
Working concentrations and stock solutions
... Most antibiotics should be ordered as the salt or HCl preparation, i.e., “ampicillin-sodium salt” or “tetracycline-HCl”. These dissolve much easier than the other preparations. ...
... Most antibiotics should be ordered as the salt or HCl preparation, i.e., “ampicillin-sodium salt” or “tetracycline-HCl”. These dissolve much easier than the other preparations. ...
Protein conformational changes induced by adsorption onto material
... they may release ions [44]. Even glasses exchange protons (surface pKa) and may release some ions in water [45, 46]. The reorganization of polymers explains the large amounts of protein that can be adsorbed on certain hydrogels [47] or multilayered polyelectrolyte films [48]. It would therefore be i ...
... they may release ions [44]. Even glasses exchange protons (surface pKa) and may release some ions in water [45, 46]. The reorganization of polymers explains the large amounts of protein that can be adsorbed on certain hydrogels [47] or multilayered polyelectrolyte films [48]. It would therefore be i ...