This is the accepted version of the following article:
... have several advantages in comparison with standard column separation procedures [8]. Separation techniques using the magnetically active microparticles are fast, gentle, easily automated and applicable in a wide range of disciplines [9]. Magnetic particles coated with antibody molecules are incubat ...
... have several advantages in comparison with standard column separation procedures [8]. Separation techniques using the magnetically active microparticles are fast, gentle, easily automated and applicable in a wide range of disciplines [9]. Magnetic particles coated with antibody molecules are incubat ...
Local protein synthesis in neuronal axons: why and
... there are many variations of the original three-chamber design, in which the dissociated neurons are plated in the central, or “proximal” compartment. Neuronal cell bodies and proximal neurites remain in the proximal chamber, and only the long neurites (which are likely to be axons) can pass through ...
... there are many variations of the original three-chamber design, in which the dissociated neurons are plated in the central, or “proximal” compartment. Neuronal cell bodies and proximal neurites remain in the proximal chamber, and only the long neurites (which are likely to be axons) can pass through ...
Insect Biochemistry 15:
... Triton X-IO0 was added to the filtrate to a final concentration of 0.5% (w/v). After stirring for 30 min, the solution was centrifuged at 20,000g and the pellet discarded. Precipitation with ammonium sulphate. A solution of saturated ammonium sulphate was added to the supernatant to bring the satura ...
... Triton X-IO0 was added to the filtrate to a final concentration of 0.5% (w/v). After stirring for 30 min, the solution was centrifuged at 20,000g and the pellet discarded. Precipitation with ammonium sulphate. A solution of saturated ammonium sulphate was added to the supernatant to bring the satura ...
Determination and Quantification of Molecular Interactions in Protein
... structure, the protein will assume different structures along the polymer chain. The geometry can be arranged as α-helix, β-sheet or as turns and is coiled and stabilized by hydrogen bonds, van der Waals, electrostatic, hydrophobic and disulfide interactions. The tertiary structure is the overall sh ...
... structure, the protein will assume different structures along the polymer chain. The geometry can be arranged as α-helix, β-sheet or as turns and is coiled and stabilized by hydrogen bonds, van der Waals, electrostatic, hydrophobic and disulfide interactions. The tertiary structure is the overall sh ...
pGEX-5X-3 GST Expression Vector
... Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below. Site-specific proteolysis of fusion proteins: Separat ...
... Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below. Site-specific proteolysis of fusion proteins: Separat ...
GFP-labelled Rubisco and aspartate aminotransferase are present
... a 4-line Argon ion laser and emission was detected by collecting light between 500 and 600 nm. CFP was excited at 458 nm and emission was collected between 500 and 600 nm. Chlorophyll was excited at 633 nm and emission was detected between 660 and 700 nm. DIC images were generated by collecting tran ...
... a 4-line Argon ion laser and emission was detected by collecting light between 500 and 600 nm. CFP was excited at 458 nm and emission was collected between 500 and 600 nm. Chlorophyll was excited at 633 nm and emission was detected between 660 and 700 nm. DIC images were generated by collecting tran ...
Chapter 4: Solution Chemistry and the Hydrosphere
... Li > K> Ba > Sr > Ca > Na Active metals react directly with water: The active metal replaces the less active by going into solution as an ion with hydroxide, OH–, and the H comes out as hydrogen gas, H2(g). active metal + H2O(l) metal hydroxide + H2(g) 1. Ca(s) + H2O(l) 2. Na(s) + H2O(l) 3. Fe ...
... Li > K> Ba > Sr > Ca > Na Active metals react directly with water: The active metal replaces the less active by going into solution as an ion with hydroxide, OH–, and the H comes out as hydrogen gas, H2(g). active metal + H2O(l) metal hydroxide + H2(g) 1. Ca(s) + H2O(l) 2. Na(s) + H2O(l) 3. Fe ...
IMMUNOLOGIC METHODS шкільна
... a. Zone-proteins separated by surface charge; used to quantitate serum protein and immunoglobulins. ...
... a. Zone-proteins separated by surface charge; used to quantitate serum protein and immunoglobulins. ...
Chapter 1 All Things Noncovalent
... Conversely, the stability of charge separation in solution is greatly enhanced, but the binding strength of the interaction is greatly reduced. As a result the removal of iodide anion from 1.1 in aqueous solution does not result in any covalent bond cleavage. It is for this same reason that the role ...
... Conversely, the stability of charge separation in solution is greatly enhanced, but the binding strength of the interaction is greatly reduced. As a result the removal of iodide anion from 1.1 in aqueous solution does not result in any covalent bond cleavage. It is for this same reason that the role ...
NMR spectroscopy brings invisible protein states into
... atomic descriptions of static three-dimensional structures. Yet pictures of globular protein molecules occupying single rigid conformations have long been known to be insufficient for obtaining a complete understanding of function. Instead, an appreciation of the conformational fluctuations such bio ...
... atomic descriptions of static three-dimensional structures. Yet pictures of globular protein molecules occupying single rigid conformations have long been known to be insufficient for obtaining a complete understanding of function. Instead, an appreciation of the conformational fluctuations such bio ...
The taste perception is an important function for living organisms to
... 3. Research characteristics (incl. originality and creativity) ...
... 3. Research characteristics (incl. originality and creativity) ...
Design and Evolution of Artificial M13 Coat Proteins
... The design and evolution of ACP-7 depended on the use of a phagemid system in which all the wild-type phage coat proteins were supplied by a helper phage. As a consequence, non-functional coat proteins could be selected, provided they incorporated into the wild-type coat without signi®cantly impairi ...
... The design and evolution of ACP-7 depended on the use of a phagemid system in which all the wild-type phage coat proteins were supplied by a helper phage. As a consequence, non-functional coat proteins could be selected, provided they incorporated into the wild-type coat without signi®cantly impairi ...
Corn Bt 176 - Biotechnology Philippines
... The glufosinate ammonium inhibits the glutamine synthetase in plants, resulting in an accumulation of ammonia in plant tissues which leads to its death. The expression of the PAT protein protects the plant from the herbicide glufosinate ammonium. Safety of the Expressed Proteins Toxicity studies con ...
... The glufosinate ammonium inhibits the glutamine synthetase in plants, resulting in an accumulation of ammonia in plant tissues which leads to its death. The expression of the PAT protein protects the plant from the herbicide glufosinate ammonium. Safety of the Expressed Proteins Toxicity studies con ...
Two-Dimensional DNA Gel Electrophoresis Mapping: a Novel
... DNA fragments, and these techniques were mainly applied to the study of genomic changes and variation in the field of medicine. A novel 2-DGE method that can generate a highresolution DNA map for resolving bacterial diversity in complex environmental soil systems has been suggested. The high-resolut ...
... DNA fragments, and these techniques were mainly applied to the study of genomic changes and variation in the field of medicine. A novel 2-DGE method that can generate a highresolution DNA map for resolving bacterial diversity in complex environmental soil systems has been suggested. The high-resolut ...
Jake Wang - Critical Review of the Rosetta Algorithm
... for i+1..j-1. In fact, the optimal structure on i+1..j-1 and its score s(i+1, j-1) are independent of anything else that is built on top of it. Thus for the first scenario, S(i, j) = S(i+1, j-1) +1 since i and j can base pair. The same recursive methods apply for the other cases. In case 2, S(i,j) = ...
... for i+1..j-1. In fact, the optimal structure on i+1..j-1 and its score s(i+1, j-1) are independent of anything else that is built on top of it. Thus for the first scenario, S(i, j) = S(i+1, j-1) +1 since i and j can base pair. The same recursive methods apply for the other cases. In case 2, S(i,j) = ...
Document
... # These centrifuges are run in batch to isolate small volumes of biological macromolecules or cells. ...
... # These centrifuges are run in batch to isolate small volumes of biological macromolecules or cells. ...
Solid state NMR assignment of a whole virus particle
... the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alternative spectroscopic experiments. The helical bundle symmetry around the D ...
... the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alternative spectroscopic experiments. The helical bundle symmetry around the D ...
Cold Shock Proteins
... dramatically reduced and only 28 proteins were detected from two-dimensional gel autoradiograms. These proteins were grouped according to the rate of synthesis: those whose differential rate of synthesis decreased, those whose differential rate of synthesis stayed the same and those whose differenti ...
... dramatically reduced and only 28 proteins were detected from two-dimensional gel autoradiograms. These proteins were grouped according to the rate of synthesis: those whose differential rate of synthesis decreased, those whose differential rate of synthesis stayed the same and those whose differenti ...
Sec14p-like proteins regulate phosphoinositide homoeostasis and
... by the increased capacity of sec14-1ts cells to secrete invertase [12]. It is also interesting that the SFH protein most related to Sec14p by homology is not a functional Sec14p on the basis of several important criteria. Yeast cells overexpressing Sfh1p have at best a very low grade Sec14p-independ ...
... by the increased capacity of sec14-1ts cells to secrete invertase [12]. It is also interesting that the SFH protein most related to Sec14p by homology is not a functional Sec14p on the basis of several important criteria. Yeast cells overexpressing Sfh1p have at best a very low grade Sec14p-independ ...
Supplementary data - Royal Society of Chemistry
... was usually set to 5-10 M by dilution of the purified batches. Aliquots of a stock solution of substrates (in dioxane for esters 1, 3a-d and 5; in DMSO for substrates 6 and 7) were added to a final concentration of 10% dioxane or 30% DMSO, in a final volume of 500 L. The concentration of the subst ...
... was usually set to 5-10 M by dilution of the purified batches. Aliquots of a stock solution of substrates (in dioxane for esters 1, 3a-d and 5; in DMSO for substrates 6 and 7) were added to a final concentration of 10% dioxane or 30% DMSO, in a final volume of 500 L. The concentration of the subst ...
Functions of Ribosome-Associated Chaperones and their Interaction
... formation of misfolded intermediate states during protein synthesis [8]. In the current model, first the ribosome itself limits the conformational freedom of the newly synthesized polypeptide chains. As translation proceeds and the chain lengthens the influence of the ribosome, in particular on the ...
... formation of misfolded intermediate states during protein synthesis [8]. In the current model, first the ribosome itself limits the conformational freedom of the newly synthesized polypeptide chains. As translation proceeds and the chain lengthens the influence of the ribosome, in particular on the ...
O A RIGINAL RTICLE
... therefore, these enzymes account for about 30% of the world’s enzyme production [7]. The major three classes of amylase have been identified in many microorganisms, namely a- amylase, β- amylases and glucoamylases [17]. All these enzymes are belongs to Glycosides hydrolases, although some a amylase ...
... therefore, these enzymes account for about 30% of the world’s enzyme production [7]. The major three classes of amylase have been identified in many microorganisms, namely a- amylase, β- amylases and glucoamylases [17]. All these enzymes are belongs to Glycosides hydrolases, although some a amylase ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... animals were sacrificed at appropriate intervals for study of solubilized sucrase-isomaltase as described below. Enzyme solubilization. The following procedures were done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from th ...
... animals were sacrificed at appropriate intervals for study of solubilized sucrase-isomaltase as described below. Enzyme solubilization. The following procedures were done at 4°C. The animals were stunned, decapitated, and the abdominal cavity opened with prompt removal of the small intestine from th ...