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Outline Overview: The Molecules of Life Macromolecules are
Outline Overview: The Molecules of Life Macromolecules are

...  When phospholipids are added to water, they self-assemble into a bilayer, with the hydrophobic tails pointing toward the interior  The structure of phospholipids results in a bilayer arrangement found in cell membranes  Phospholipids are the major component of all ...
Design Genes with Ease Using In-Fusion® Cloning
Design Genes with Ease Using In-Fusion® Cloning

... restriction enzyme sites used to join the DNA ends. This is particularly detrimental for fusion proteins and recombinant antibodies, since the undesired amino acids may perturb structure, reduce expression, or be antigenic. We generated a seamless murine PD-L2-IgG2a Fc-IgA tailpiece fusion protein b ...
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... a. Pyruvic acid enters the mitochondrial matrix through facilitated diffusion b. There it is converted to Acetyl-Coenzyme A to enter Krebs cycle c. 1 CO2 and 1 NADH is produced in this stage per pyruvate ...
Unit 3 - Concord Carlisle High School
Unit 3 - Concord Carlisle High School

... Unit Test: Tuesday Oct 25th – 100pts ...
Cell Metabolism
Cell Metabolism

Lecture #8 - Faculty Web Sites at the University of Virginia
Lecture #8 - Faculty Web Sites at the University of Virginia

... Glutamate -> Glutamic Acid ∆ G = + 3.4 kcal Net ∆ G = -3.9 kcal; therefore both reactions occur! Carbohydrates may be oxidized to Carbon dioxide with a great yield of energy. If we couple this to a process called Oxidative Phosphorylation we can assemble ADP + Pi to form ATP. We can also couple ATP ...
Key Molecule for the Evolution of Life—Nucleic Acid
Key Molecule for the Evolution of Life—Nucleic Acid

TRANSLATION: How to make proteins?
TRANSLATION: How to make proteins?

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Overview on Reactions with Multi

... crucial for the industrial application of oxidoreductases is coenzyme regeneration.13 The results so far clearly demonstrate that cofactor regeneration is no longer an economical issue. It is known that some cofactors (pyridoxal phosphate, biotin) are tightly bound to the enzymes and are essentially ...
Unit 2 Study Guide: Carbon Compounds
Unit 2 Study Guide: Carbon Compounds

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Enzyme Assay for Glutathione S-Transferase Protocol

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Restricted rotation of the amino group of nucleic acid base

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View Essential-4 Data Sheet

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-The oxygen consumed during cellular respiration is involved

... -Where are the proteins of the electron transport chain located? -The primary role of oxygen in cellular respiration is to _____. -During aerobic respiration, H2O is formed. Where does the oxygen atom for the formation of the water come from? -In chemiosmosis, what is the most direct source of energ ...
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Chapter 16.3: Anaerobic Respiration

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Chapter 5b

... • Uses an organic molecule as the final electron acceptor • Recycles NAD / NADH for Glycolysis ...
Enzymes - Kevan Kruger
Enzymes - Kevan Kruger

... 15.Another more recent explanation of enzyme action is the "induced fit" theory. Describe the basics of this theory. 16.What is an active site, and how can it be changed? 17.Describe how different concentrations and competitive inhibitors can affect enzymes. 18.What is the role of thyroxin in the bo ...
Author - Princeton ISD
Author - Princeton ISD

... Students may think all mutations are harmful to the organism. Some students may think that the purpose of protein synthesis is to make amino acids. Since they work hand in hand, students are always hearing the terms used together. As a result, students often lose track of where amino acids originate ...
Practical 1
Practical 1

... 4. Repeate  step  1  and  2  for  RNA  sequences.   5. Repeate  step  1  and  2  for  protein  sequence  by  generating  an  amino  acid  polypeptide  of   length  100  and  retriving  the  most  over-­‐represented  amino  acid  in  t ...
ans - Gogarten Lab
ans - Gogarten Lab

... B. Cells. Life could only exist in a form compartmentalized by a lipid bilayer. C. Self-sustained metabolic system that does not require input from any other living system. D. An interacting web with intricate feedback loops to ensure homeostasis. E. Anything that can reproduce itself perfectly w ...
ภาพนิ่ง 1
ภาพนิ่ง 1

... the number and/or types of substrates that a particular enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate-limiting enzyme in many of the biochemical metabolic pathways ...
Study Guide (Chapter`s 7-10)
Study Guide (Chapter`s 7-10)

... a. glycolysis and fermentation. b. Stage 1 and Stage 2 of photosynthesis. c. glycolysis, then aerobic respiration. d. aerobic respiration, then glycolysis. In cellular respiration, a two-carbon molecule combines with a four-carbon molecule to form citric acid as part of a. glycolysis. b. carbon fixa ...
Effect of peptide chain length on amino acid and
Effect of peptide chain length on amino acid and

... seemed, at the time, that there could be a number of explanations for the differences (varying amino acid composition of the starter proteins, different peptide chain lengths and hydrolysis method used), direct comparisons of the data could not be made as the experimental conditions differed in some ...
Protein Structure Prediction The Protein Folding Problem
Protein Structure Prediction The Protein Folding Problem

... • protein structure is often described at four different scales – primary structure – secondary structure – tertiary structure – quaternary structure • don’t confuse these with Rost’s references to structure prediction in “1D”, “2D”, and “3D” ...
ppt link
ppt link

... Initiator proteins identify specific base sequences on DNA Prokaryotes – single ori site E.g E.coli - oriC Eukaryotes – multiple sites of origin (replicator) E.g. yeast - ARS (autonomously replicating sequences) ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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