Lecture#7 Microbial Biotechnology
... • Production of insulin More discoveries • 1955: Frederick Sanger (Cambridge) determines full amino acid structure of insulin (primary structure) • 1958: Frederick Sanger wins Nobel Prize in Chemistry (1980: determination of base sequences in nucleic acids shared with Walter Gilbert) • 1969: Doroth ...
... • Production of insulin More discoveries • 1955: Frederick Sanger (Cambridge) determines full amino acid structure of insulin (primary structure) • 1958: Frederick Sanger wins Nobel Prize in Chemistry (1980: determination of base sequences in nucleic acids shared with Walter Gilbert) • 1969: Doroth ...
Laboratory 3: Biological Molecules
... Proteins are a diverse group of biological molecules. They include enzymes that make reactions proceed faster. There are structural proteins that make spider webs strong and there are proteins that transport things in cells. This laboratory deals with nutritional proteins. All proteins are made up o ...
... Proteins are a diverse group of biological molecules. They include enzymes that make reactions proceed faster. There are structural proteins that make spider webs strong and there are proteins that transport things in cells. This laboratory deals with nutritional proteins. All proteins are made up o ...
Intro to Metabolism
... g. Nitrate is a “dead molecule” to us because there are no biochemical means to use it h. Luckily plants are capable of converting nitrate back into ammonia i. We as humans end up on the sidewalk of the cycle because our source of nitrogen is obtained from eating molecules produced by plants Relatio ...
... g. Nitrate is a “dead molecule” to us because there are no biochemical means to use it h. Luckily plants are capable of converting nitrate back into ammonia i. We as humans end up on the sidewalk of the cycle because our source of nitrogen is obtained from eating molecules produced by plants Relatio ...
The lower Vmax is consistent with the mutation resulting in a
... binding to the mRNA and release of the protein from the polyC/U region, allowing translation initiation. You have identified Ser395 as a putative phosphorylation site for S6 protein kinase. To test this hypothesis, you generate an αCP1S395A point mutant and find that it binds polyC/U but fails to un ...
... binding to the mRNA and release of the protein from the polyC/U region, allowing translation initiation. You have identified Ser395 as a putative phosphorylation site for S6 protein kinase. To test this hypothesis, you generate an αCP1S395A point mutant and find that it binds polyC/U but fails to un ...
`The potent poison quite o`ercrows my spirit`
... prey's cardiac system, mainly to lower the blood pressure. Phospholipase A2 causes hemolysis by lysing the phospholipid cell membranes of red blood cells.[3] Snake venom inhibits cholinesterase to make the prey lose muscle control. Hyaluronidase increases tissue permeability to increase the rate tha ...
... prey's cardiac system, mainly to lower the blood pressure. Phospholipase A2 causes hemolysis by lysing the phospholipid cell membranes of red blood cells.[3] Snake venom inhibits cholinesterase to make the prey lose muscle control. Hyaluronidase increases tissue permeability to increase the rate tha ...
Document
... 17.6 – Hydration of Aldehydes and Ketones Electronic (how stable is the carbonyl – how positive is the carbon?) ...
... 17.6 – Hydration of Aldehydes and Ketones Electronic (how stable is the carbonyl – how positive is the carbon?) ...
DNA
... What controls inherited traits? Every inherited trait is controlled by one or more proteins 2. What controls the production of proteins? DNA structure controls the production of proteins. 3. Define a gene. A section of DNA which is used as the blueprint for the production of a protein is a gene. 4. ...
... What controls inherited traits? Every inherited trait is controlled by one or more proteins 2. What controls the production of proteins? DNA structure controls the production of proteins. 3. Define a gene. A section of DNA which is used as the blueprint for the production of a protein is a gene. 4. ...
Problem set answers
... 4. AlphaCP1 is a polyC/U binding protein that binds to polyC/U-containing 3' UTR regions of certain mRNA species to stabilize the message and suppress translation. Phosphorylation of aCP1 by S6 protein kinase is thought to result in reduced binding to the mRNA and release of the protein from the po ...
... 4. AlphaCP1 is a polyC/U binding protein that binds to polyC/U-containing 3' UTR regions of certain mRNA species to stabilize the message and suppress translation. Phosphorylation of aCP1 by S6 protein kinase is thought to result in reduced binding to the mRNA and release of the protein from the po ...
Ch18.doc
... 5. Both alanine and lactate have to be converted to pyruvate. From lactate, it is the lactate dehydrogenase reaction (1 NADH), then pyruvate gets oxidized by pyruvate dehydrogenase (1 NADH) and one turn of the CAC: yielding 3NADH, 1FADH2 and 1 GTP. Converting NADH and FADH2 to ATPs we use 1 NADH = 2 ...
... 5. Both alanine and lactate have to be converted to pyruvate. From lactate, it is the lactate dehydrogenase reaction (1 NADH), then pyruvate gets oxidized by pyruvate dehydrogenase (1 NADH) and one turn of the CAC: yielding 3NADH, 1FADH2 and 1 GTP. Converting NADH and FADH2 to ATPs we use 1 NADH = 2 ...
AP-Bio-exam-review-outline-may-2
... active site, causes conformational change in enzyme, Allosteric enzyme Negative feedback - product of enzyme reaction can also be inhibitor of same reaction Lipids Function: energy storage, structure, sex hormones like testosterone Examples: triglycerides (fats, saturated, unsaturated)- 1 glyc ...
... active site, causes conformational change in enzyme, Allosteric enzyme Negative feedback - product of enzyme reaction can also be inhibitor of same reaction Lipids Function: energy storage, structure, sex hormones like testosterone Examples: triglycerides (fats, saturated, unsaturated)- 1 glyc ...
... iv) Draw to the right of the arrow labeled ’iv’ the products of the reaction if this peptide was treated with a protease. (2 pts) v) What would be the effect of treating this peptide with a protein kinase? Please complete the drawing that I have started for you to the right of the arrow labeled ’v’. ...
Carbohydrates and Lipids
... • Saturated Fat: a lipid that is composed of saturated fatty acids with a single bonds in their hydrocarbon chain • Unsaturated Fat: a lipid that is composed of unsaturated fatty acids with double bounds in their hydrocarbon chain ...
... • Saturated Fat: a lipid that is composed of saturated fatty acids with a single bonds in their hydrocarbon chain • Unsaturated Fat: a lipid that is composed of unsaturated fatty acids with double bounds in their hydrocarbon chain ...
Chapter 2 - Molecules of Life (Biochemistry) Periodic Table of
... • Break larger molecule into smaller parts! ...
... • Break larger molecule into smaller parts! ...
Taícia P. Fill1, Edson Rodrigues Filho1
... endophytic fungus Penicillium brasilianum which present important antiparasitic and antibacteriological activities[1]. In the present work, the biosynthesis of these compounds was evaluated through the addition of labeled precursors in the culture medium. In this sense, 13C Isotopically labeled phen ...
... endophytic fungus Penicillium brasilianum which present important antiparasitic and antibacteriological activities[1]. In the present work, the biosynthesis of these compounds was evaluated through the addition of labeled precursors in the culture medium. In this sense, 13C Isotopically labeled phen ...
Forever Lite® Meal Replacement – Chocolate, Vanilla
... nutritious kick of vitamins, minerals, proteins and carbohydrates. Rich in proteins for bodybuilding, growth and repair, two shakes a day provide most of the Daily Value of vitamins and minerals. Each shake contains many amino acids (including all 8 essential ones). To protect against free radicals, ...
... nutritious kick of vitamins, minerals, proteins and carbohydrates. Rich in proteins for bodybuilding, growth and repair, two shakes a day provide most of the Daily Value of vitamins and minerals. Each shake contains many amino acids (including all 8 essential ones). To protect against free radicals, ...
Triacylglycerol Metabolism Gone Bad: A major cause of disease
... It releases fatty acids and glycerol (produced by adipose lipases). •In the fed state triacylglycerol is transported in the blood as a lipoprotein complex. In the blood the triacylglycerol is hydrolyzed to produce fatty acids and glycerol (lipoprotein ...
... It releases fatty acids and glycerol (produced by adipose lipases). •In the fed state triacylglycerol is transported in the blood as a lipoprotein complex. In the blood the triacylglycerol is hydrolyzed to produce fatty acids and glycerol (lipoprotein ...
What are Vitamins?
... – Clinical research has shown no evidence of carcinogenic effects with administration of alpha lipoic acid. Serious side effects have not been observed, even at high doses. Minor side effects include skin reactions and gastrointestinal effects, such as nausea and vomiting. ...
... – Clinical research has shown no evidence of carcinogenic effects with administration of alpha lipoic acid. Serious side effects have not been observed, even at high doses. Minor side effects include skin reactions and gastrointestinal effects, such as nausea and vomiting. ...
medbiochem exam, 1999
... A mechanism to allow mRNA transcription by RNA polymerase III. B. A highly methylated 5' cap structure. C. An unusually long poly (A) tail. D. A mechanism to allow cap-independent translation. Answer: D 31. All of the following are likely to occur after eating a large amount of carbohydrate EXCEPT: ...
... A mechanism to allow mRNA transcription by RNA polymerase III. B. A highly methylated 5' cap structure. C. An unusually long poly (A) tail. D. A mechanism to allow cap-independent translation. Answer: D 31. All of the following are likely to occur after eating a large amount of carbohydrate EXCEPT: ...
WATER - Biology Mad
... 8. Polysaccharides are carbohydrates made of long chains of sugars. Starch, which is found in plants only, is a polysaccharide. Starch actually consists of two different molecules: amylose (a long chain of ‘poly maltoses’, thus only having α-1:4 glycosidic bonds) and amylopectin (which has a branch ...
... 8. Polysaccharides are carbohydrates made of long chains of sugars. Starch, which is found in plants only, is a polysaccharide. Starch actually consists of two different molecules: amylose (a long chain of ‘poly maltoses’, thus only having α-1:4 glycosidic bonds) and amylopectin (which has a branch ...
Faculty of Science, IUG
... Date:2/12 /2005 Name----------- & NO.----------Answer the following I- Sketch the titration curve, calculate pI and determine the regions of the buffer capacity of ASP. The pK values of its Alfa COOH, Alfa amino , and Beta COOH groups are 2.1, 9.2, and 3.9 respectively. (6pts). ...
... Date:2/12 /2005 Name----------- & NO.----------Answer the following I- Sketch the titration curve, calculate pI and determine the regions of the buffer capacity of ASP. The pK values of its Alfa COOH, Alfa amino , and Beta COOH groups are 2.1, 9.2, and 3.9 respectively. (6pts). ...
N-terminal derivatization for de novo sequencing
... 7. Guanidination. Conversion of carboxy-terminal lysine residues (from tryptic digestion) to homoarginines prevents unwanted sulfonate tagging of these residues, increases their basicities and can improve the selective formation of y-series ions; and so the following procedure may be used on peptide ...
... 7. Guanidination. Conversion of carboxy-terminal lysine residues (from tryptic digestion) to homoarginines prevents unwanted sulfonate tagging of these residues, increases their basicities and can improve the selective formation of y-series ions; and so the following procedure may be used on peptide ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.