a simple integrated diagnostic platform for dna testing of chlamydia
... ratio. In current work, beads coated with polyhistidine residues are used for DNA extraction. Bead surface charge is positive at acidic pH and negative at alkaline pH, allowing elution of captured DNA directly into LAMP mixture. Colorimetric detection of DNA amplification obviates the need for sophi ...
... ratio. In current work, beads coated with polyhistidine residues are used for DNA extraction. Bead surface charge is positive at acidic pH and negative at alkaline pH, allowing elution of captured DNA directly into LAMP mixture. Colorimetric detection of DNA amplification obviates the need for sophi ...
Modeling of Protein-Small Molecule Complexes
... separated by more than three atoms. The non-1,4 VDW values were minimal with a difference of 0.1948 Kcal/mol, favoring the energy minimized conformation. The 1,4 VDW values represent the energy for the through-space interaction of atoms separated by two atoms. The difference between the two is large ...
... separated by more than three atoms. The non-1,4 VDW values were minimal with a difference of 0.1948 Kcal/mol, favoring the energy minimized conformation. The 1,4 VDW values represent the energy for the through-space interaction of atoms separated by two atoms. The difference between the two is large ...
8.2 HL Respiration pPractice Questions
... □ Outline the process of glycolysis, including phosphorylation, lysis, oxidation and ATP formation. 2 In the cytoplasm, one hexose sugar is converted into two three-carbon atom compounds (pyruvate) with a net gain of two ATP and two NADH + H+. □ Draw and label a diagram showing the structure of a mi ...
... □ Outline the process of glycolysis, including phosphorylation, lysis, oxidation and ATP formation. 2 In the cytoplasm, one hexose sugar is converted into two three-carbon atom compounds (pyruvate) with a net gain of two ATP and two NADH + H+. □ Draw and label a diagram showing the structure of a mi ...
PCR Polymerase Chain Reaction
... • Cancer Detection : Scientists are working on ways to use RT-PCR in cancer detection to help improve prognosis, and monitor response to therapy. Circulating tumor cells produce unique mRNA transcripts depending on the type of cancer. • RT-PCR is commonly used in studying the genomes of viruses whos ...
... • Cancer Detection : Scientists are working on ways to use RT-PCR in cancer detection to help improve prognosis, and monitor response to therapy. Circulating tumor cells produce unique mRNA transcripts depending on the type of cancer. • RT-PCR is commonly used in studying the genomes of viruses whos ...
CH_16_5_16_6_Enzymes_Enzyme_Action
... fits the shape of the reacting molecule called a substrate • contains amino acid R groups that bind the substrate • releases products when the reaction is complete On the surface of an enzyme, a small region called an active site binds a substrate and catalyzes a reaction of that substrate. Chemistr ...
... fits the shape of the reacting molecule called a substrate • contains amino acid R groups that bind the substrate • releases products when the reaction is complete On the surface of an enzyme, a small region called an active site binds a substrate and catalyzes a reaction of that substrate. Chemistr ...
Abbreviations and Symbols for Chemical Names of Special Interest
... acids is unknown, but the last nine amino acids are in known order with alanine carrying the free carboxyl group. If the direction of the link must be specfied, this may be done with a n arrow thus (+), the point of the arrow indicating the nitrogen of the peptide bond.. .CO - + N H . . Example: Gly ...
... acids is unknown, but the last nine amino acids are in known order with alanine carrying the free carboxyl group. If the direction of the link must be specfied, this may be done with a n arrow thus (+), the point of the arrow indicating the nitrogen of the peptide bond.. .CO - + N H . . Example: Gly ...
CHAPTER 11 Mechanism of Enzyme Action
... 3. Enzymology, the study of enzymes (coined 1878; Greek: en, in; zyme, yeast), fermentation: glucose -> ethanol 12 enzyme-catalyzed steps 4. James Summer, 1926, crystallized urease from jack bean, shown to be a protein 5. Other catalysts, i.e. ribozymes (peptide-bond formation; “RNA-world”), only ...
... 3. Enzymology, the study of enzymes (coined 1878; Greek: en, in; zyme, yeast), fermentation: glucose -> ethanol 12 enzyme-catalyzed steps 4. James Summer, 1926, crystallized urease from jack bean, shown to be a protein 5. Other catalysts, i.e. ribozymes (peptide-bond formation; “RNA-world”), only ...
1. ATP powers cellular processes by coupling exergonic and
... the habitat necessary for prokaryotic reproduction the primordial sea the air in which life began volcanic activity which stimulated terrestrial mutations ...
... the habitat necessary for prokaryotic reproduction the primordial sea the air in which life began volcanic activity which stimulated terrestrial mutations ...
ATP
... A FLAVIN-BASED LIGHT ENERGY CONVERTER, COULD HAVE ARISEN The main facts that form the basis of this hypothesis are: …and yet another hypothetical option ...
... A FLAVIN-BASED LIGHT ENERGY CONVERTER, COULD HAVE ARISEN The main facts that form the basis of this hypothesis are: …and yet another hypothetical option ...
(Vitis vinifera L.) berries - Oxford Academic
... and glutamine synthetase showed little change in abundance. The abundance of several other enzymes involved in amino acid metabolism increased during the later stages of ripening (notably alanine aminotransferase and mitochondrial aspartate aminotransferase). In contrast, SDS-PAGE analysis of the se ...
... and glutamine synthetase showed little change in abundance. The abundance of several other enzymes involved in amino acid metabolism increased during the later stages of ripening (notably alanine aminotransferase and mitochondrial aspartate aminotransferase). In contrast, SDS-PAGE analysis of the se ...
Amino Acids, Proteins, and Enzymes
... fits the shape of the reacting molecule called a substrate • contains amino acid R groups that bind the substrate • releases products when the reaction is complete On the surface of an enzyme, a small region called an active site binds a substrate and catalyzes a reaction of that substrate. Chemistr ...
... fits the shape of the reacting molecule called a substrate • contains amino acid R groups that bind the substrate • releases products when the reaction is complete On the surface of an enzyme, a small region called an active site binds a substrate and catalyzes a reaction of that substrate. Chemistr ...
Student notes in ppt
... by both reversible phosphorylation (the active conformation is dephosphorylated) and allosteric mechanisms (citrate binding stimulates activity, palmitoyl-CoA inhibits activity). Fatty acid synthase - this large multi-functional enzyme is responsible for catalyzing a series of reactions that sequent ...
... by both reversible phosphorylation (the active conformation is dephosphorylated) and allosteric mechanisms (citrate binding stimulates activity, palmitoyl-CoA inhibits activity). Fatty acid synthase - this large multi-functional enzyme is responsible for catalyzing a series of reactions that sequent ...
Questions
... Evidence for in situ synthesis of cell wall microfibrils (cont) e. Demonstration that at least some, if not all chitin is synthesized, most likely, in a vectorial way as demonstrated by unilateral formation of chitin by isolated plasma membrane. Pretreatment of protoplasts with gluteradehyde before ...
... Evidence for in situ synthesis of cell wall microfibrils (cont) e. Demonstration that at least some, if not all chitin is synthesized, most likely, in a vectorial way as demonstrated by unilateral formation of chitin by isolated plasma membrane. Pretreatment of protoplasts with gluteradehyde before ...
Powerpoint Presentation: The Origins of Life
... Amino acids into polypeptides, could have occurred when dry or highly concentrated monomers are heated Condensation reactions take place forming peptide bonds between amino acids or phosphodiester bonds form between nucleotides. ...
... Amino acids into polypeptides, could have occurred when dry or highly concentrated monomers are heated Condensation reactions take place forming peptide bonds between amino acids or phosphodiester bonds form between nucleotides. ...
lecture7
... Fatty acid synthesis starts with the carboxylation of acetyl CoA to malonyl CoA. This irreversible reaction is the committed step in fatty acid synthesis. The synthesis of malonyl CoA is catalyzed by acetyl CoA carboxylase, which contains a biotin prosthetic group. The carboxyl group of biotin is co ...
... Fatty acid synthesis starts with the carboxylation of acetyl CoA to malonyl CoA. This irreversible reaction is the committed step in fatty acid synthesis. The synthesis of malonyl CoA is catalyzed by acetyl CoA carboxylase, which contains a biotin prosthetic group. The carboxyl group of biotin is co ...
File - western undergrad. by the students, for the students.
... biological membranes. In water, glycerophospholipids and ceramides spontaneously assemble into bilayers to hide or protect the hydrophobic groups from the polar solvent. The hydrophilic “head” groups are on the outside of the bilayer, next to the solvent, while the hydrophobic “tail” groups form the ...
... biological membranes. In water, glycerophospholipids and ceramides spontaneously assemble into bilayers to hide or protect the hydrophobic groups from the polar solvent. The hydrophilic “head” groups are on the outside of the bilayer, next to the solvent, while the hydrophobic “tail” groups form the ...
Slides - Pages
... Biochemical Review An amino acid consists of: a central carbon atom C an amino group NH2 a carboxyl group COOH a hydrogen atom H and a side chains R ...
... Biochemical Review An amino acid consists of: a central carbon atom C an amino group NH2 a carboxyl group COOH a hydrogen atom H and a side chains R ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.