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RNA polymerase - Industrial ISD
RNA polymerase - Industrial ISD

... spliceosome. (2) Within the spliceosome, snRNA base-pairs with nucleotides at the ends of the intron. (3) The RNA transcript is cut to release the intron, and the exons are spliced together; the spliceosome then comes apart, releasing mRNA, which now contains only exons. Copyright © 2002 Pearson Edu ...
Ex2 answers
Ex2 answers

... need to be transcribed by jellyfish RNA polymerase. (Remember, a promoter is a binding site for RNA polymerase and therefore a start signal for transcription.) (e, 3 pts) Must your plasmid contain a promoter that works in E. coli? If yes, state which specific gene on the plasmid will be controlled b ...
Anti-PRPS1 antibody
Anti-PRPS1 antibody

... The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. アプリケーション ...
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Reverse transcription-pcr (rt-pcr)
Reverse transcription-pcr (rt-pcr)

... RT-PCR can be performed in two steps, with an RT reaction occurring first, then using that product as a template in a PCR reaction. Alternatively, RT-PCR can be carried out in one step with both reaction occurring in one tube. ...


... Ala Ile Asn (pH between 0 and 10), or polar. Some amino acids belong to Trp His Phe Gln more than one set, e.g. all charged residues are also polar. Thr a) Explain, with reference to its sidechain atoms, where you Ser would place Tyrosine (Tyr) on this chart (2 pts). Polar b) One amino acid is very ...
Biology * Introduction to Organic Chemistry
Biology * Introduction to Organic Chemistry

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Preparation of Escherichia coli

Enzyme Web Quest KEY
Enzyme Web Quest KEY

... Lastly, the enzyme lets go. The enzyme is not changed and is ready to do another reaction. The substrate is different now, and it is called the product. 10. How does temperature help control enzymes? Enzymes change shape as temperature changes, so temperature changes can mess up the enzyme and cause ...
fillable MS-Word version of the In Silico Kinase Match
fillable MS-Word version of the In Silico Kinase Match

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DNA Replication

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Biochemistry of connective tissue

... coil conformation made up of 830 amino acids. • Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array. The amino acid responsible for these cross-links is lysine. ...
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Clinical Biochemistry
Clinical Biochemistry

... constituent monosaccharides or oligosaccharides. They range in structure from linear to highly branched. Examples include storage polysaccharides such as starch and glycogen, and structural polysaccharides such as cellulose and chitin. Polysaccharides are often quite heterogeneous, containing slight ...
Free Amino Acid and Reducing Sugar Composition of Pandan
Free Amino Acid and Reducing Sugar Composition of Pandan

www.studyguide.pk
www.studyguide.pk

Amino acid
Amino acid

... • Nucleotide: consists of a sugar with a fivecarbon ring bonded to a nitrogencontaining base and one, two, or three phosphate groups – Example: ATP (adenosine triphosphate); an energy carrier in cells ...
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Fermentation and Cellular Respiration
Fermentation and Cellular Respiration

... that are bound to membranes. In the case of prokaryotic cells, these membranes are cell membranes, while within most eukaryotic cells, the membranes involved are the inner folded membranes or cristae of mitochondria. The electron transport chain or system involves a series of membrane-bound proteins ...
The investigation of enzymes structure, physical
The investigation of enzymes structure, physical

... Without enzymes, life as we know it would not be possible. As the biocatalysts that regulate the rates at which all physiologic processes take place, enzymes occupy central roles in health and disease. While in health all physiologic processes occur in an ordered, regulated manner and homeostasis is ...
Evaluation of volatile compounds produced by Lactobacillus
Evaluation of volatile compounds produced by Lactobacillus

... Chromatographic analysis Volatile compounds retained on the fiber-coating phase were thermally desorbed in the injection port (250 °C, splitless mode) equipped with a narrow-bore glass liner (Supelco, Bellefonte, USA) of a gas chromatograph (PerkinElmer model 9000, USA). The compounds were separated ...
Translational Initiation in Eukaryotes
Translational Initiation in Eukaryotes

... (aspartate-glutamate-alanineaspartate) characteristic of RNA helicases • RNA helicase activity was demonstrated (right panel) and found to require ATP and to be stimulated by another protein, eIF4B ...
07 Enzyme Catalysis
07 Enzyme Catalysis

...  enzyme’s catalytic site; substrate fits into active site ...
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... Bogus! The free energy of the starting materials and products differ for the two lines. Enzymes cannot change the free energy of substrates or products; they can only change the activation energy (difference in free energy between the substrate and the high-energy intermediate). [This question is ba ...
Sodium Accelerated Buffer System
Sodium Accelerated Buffer System

... The continual increase in sample numbers in busy labs means that it is often difficult for Quality Control or Contract Analysis Labs to maintain short turnaround times, particularly when instruments are already running at full capacity. To address the need for faster analysis while retaining the qua ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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