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Pyruvate Dehydrogenase Complex (PDC)
Pyruvate Dehydrogenase Complex (PDC)

... oxidative phosphorylation. 4. Can also be used for gluconeogenesis to form G6P 5. 1 molecule of G6P can be converted via 6 cycles of PPP and gluconeogenesis to 6 CO2 molecules and generate 12 NADPH molecules. 6. Flux through PPP (rate of NADPH production) is controlled by the glucose-6-phosphate deh ...
Why is asymmetric synthesis important?
Why is asymmetric synthesis important?

... improvement before mankind can compete with nature’s synthetic processes. Nature’s high molecular weight systems ...
Properties of Enzymes Lab
Properties of Enzymes Lab

Topic 8: ACIDS and BASES
Topic 8: ACIDS and BASES

N8113S N8113S
N8113S N8113S

... MBP with the specific protease Genenase™ I (NEB #P8075). MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin. Source: NEB 10-beta Competent E. coli (pMAL-p5G) Su ...
1. The graph shows the relative levels of Cdk1 and cyclin B
1. The graph shows the relative levels of Cdk1 and cyclin B

Amino acids - Industrial ISD
Amino acids - Industrial ISD

... • The enzymes that digest starch cannot hydrolyze the beta linkages in cellulose. • Cellulose in our food passes through the digestive tract and is eliminated in feces as “insoluble fiber”. • As it travels through the digestive tract, it abrades the intestinal walls and stimulates the secretion of ...
Reactive Oxygen Species
Reactive Oxygen Species

Directed mutagenesis of the Trypanosoma cruzi trans
Directed mutagenesis of the Trypanosoma cruzi trans

... only in retention of sialidase activity (Figure 4). The level of sialyltransferase activity of the P to Q mutant was approximately 25% that of wild-type TS (Figure 2). We had previously described the inability to express enzyme activity from constructs expressing only the catalytic domains (i.e., wi ...
Adenovirus-associated Virus Structural Protein Sequence Homology
Adenovirus-associated Virus Structural Protein Sequence Homology

... (unlike the helper adenovirus proteins). The amino acid composition data presented here provide more complete evidence demonstrating the similarity of the amino acid compositions of the three virion proteins. The relative concentration (mole percentage) of each amino acid is also similar for each of ...
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High Alcohol Fermentations: How to Manage Primary and
High Alcohol Fermentations: How to Manage Primary and



... A phospholipid replaces the fatty acid at position 1 with a phosphate group that may link to other groups (such as choline) 5. (10 pts) Please do any one of the following three questions: Choice A: Briefly describe the role of the hydrophobic effect on the formation of phospholipid bilayers and mice ...
An Introduction to Energy, Enzymes, and Metabolism
An Introduction to Energy, Enzymes, and Metabolism

... within molecules, or the transfer of electrons from one atom to another. Every living cell continuously performs thousands of such chemical reactions to sustain life. The term metabolism is used to describe the sum total of all chemical reactions that occur within an organism. The term also refers t ...
INSIDER`S GUIDE Interpretation and treatment: Organic acid
INSIDER`S GUIDE Interpretation and treatment: Organic acid

RESPIRATION: SYNTHESIS OF ATP
RESPIRATION: SYNTHESIS OF ATP

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structure-tertiary-text

... structure then form domains, which fold independently of the rest of the protein; ...
Carbohydrate Metabolism - BITS Academic Resource Center
Carbohydrate Metabolism - BITS Academic Resource Center

... Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis, enter into the Kreb's cycle and oxidative phosphorylation to generate ATP.  Oxidative phosphory ...
The CoFactor database: organic cofactors in enzyme catalysis
The CoFactor database: organic cofactors in enzyme catalysis

... NC-IUBMB and Webb,E.C. (1992) Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB). Enzyme nomenclature. ...
9. proteins i
9. proteins i

... Each amino acid is a nitrogenous compound having both an acidic carboxyl (— COOH) and a basic amino (— NH2) group. R stands for the side chains that are different for each amino acid. R can be as simple as a hydrogen atom (H) or a methyl group (— CH3) or a more complex structure. The first carbon is ...
the chemistry of life: organic and biological chemistry
the chemistry of life: organic and biological chemistry

... Although biological systems are almost unimaginably complex, they are nevertheless constructed of molecules of quite modest size, put together in nature to form a host of complex, interacting structures. The example of phenylalanine and PKU illustrates the point that to understand biology, we need t ...
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of

... genes exhibit a preference for using A þ T rich codons (Crozier and Crozier, 1993). This phenomena seems characteristic of the Insecta, while the A þ T content is significantly lower in crustaceans than in insects (Garcia-Machado et al., 1999). Because of the codon preference, the base composition i ...
UNIT 3 – CELLULAR ENERGETICS Chapter 9
UNIT 3 – CELLULAR ENERGETICS Chapter 9

... each stage occurs. Describe how the carbon skeleton of glucose changes as it proceeds through glycolysis. Explain why ATP is required for the preparatory steps of glycolysis. Identify where substrate-level phosphorylation and the reduction of NAD+ occur in glycolysis. Describe where pyruvate is oxid ...
Myoglobin from equine skeletal muscle (M0630)
Myoglobin from equine skeletal muscle (M0630)

... Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It posesses no disulfide bridges or free -SH groups. Myoglobin contains 8 variously sized right-handed helical regions, joined by non-ordered or random coil regions. These 8 helices (A, B, C, D, E ...
RNA-based life forms
RNA-based life forms

... DNA are known as cytosine, adenine, guanine and thymine, whereas in RNA, thymine is replaced with uracil. These two differences are chemically very significant and are almost certainly the reason why DNA is now the predominant macromolecule for storage of genetic information. The presence of the 2´- ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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