Benchmarking two commonly used Saccharomyces
... more than 50% of the analyzes with CEN.PK series being the most popular (approx. 37%-CEN.PK vs. 24%-S288c and its derivatives) (Kim et al., 2012; Hong and Nielsen, 2012). The importance of the two strains as cell factories is further substantiated by an extensive multi-laboratory efforts which were ...
... more than 50% of the analyzes with CEN.PK series being the most popular (approx. 37%-CEN.PK vs. 24%-S288c and its derivatives) (Kim et al., 2012; Hong and Nielsen, 2012). The importance of the two strains as cell factories is further substantiated by an extensive multi-laboratory efforts which were ...
Altering protein specificity: techniques and applications
... rapid protocols for screening and/or selection of variants with the desired phenotype. In this review, which is intended to be illustrative rather than comprehensive, some general approaches that have ...
... rapid protocols for screening and/or selection of variants with the desired phenotype. In this review, which is intended to be illustrative rather than comprehensive, some general approaches that have ...
video slide - Point Pleasant Beach School District
... – Include both sugars and their polymers (starches) ...
... – Include both sugars and their polymers (starches) ...
S2 Protocol.
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
Translation Worksheet
... 10. New tRNA (after the initial met tRNA) comes in at the ____ site, then moves to the ___ site as it gets the polypeptide added to it. Then, after becoming uncharged it moves the ___ site and is later ________________. ...
... 10. New tRNA (after the initial met tRNA) comes in at the ____ site, then moves to the ___ site as it gets the polypeptide added to it. Then, after becoming uncharged it moves the ___ site and is later ________________. ...
Determination of the Structure and Composition of the ` Sulphur
... granule is a mycelial mass of Actinornyces bovis cemented together by a polysaccharide + protein complex and containing about 50 yo calcium phosphate. With the exception of the calcium phosphate, the granule had essentially the same composition as organisms grown in vitro. It is concluded that the ‘ ...
... granule is a mycelial mass of Actinornyces bovis cemented together by a polysaccharide + protein complex and containing about 50 yo calcium phosphate. With the exception of the calcium phosphate, the granule had essentially the same composition as organisms grown in vitro. It is concluded that the ‘ ...
Cellular Respiration
... 9.2 Glycolysis harvests chemical energy by oxidizing glucose to pyruvate • Glycolysis can occur whether or not O2 is present. o If O2 is present, the chemical energy stored in pyruvate and NADH can be extracted by the citric acid cycle, and oxidative phosphorylation o More than 75% of the original e ...
... 9.2 Glycolysis harvests chemical energy by oxidizing glucose to pyruvate • Glycolysis can occur whether or not O2 is present. o If O2 is present, the chemical energy stored in pyruvate and NADH can be extracted by the citric acid cycle, and oxidative phosphorylation o More than 75% of the original e ...
BIOMOLECULES: INTRODUCTION, STRUCTURE AND FUNCTION
... not exist”. On suggestion of one of his friends, the famous Swedish chemist Berzelius, Mulder named this “most important of all substances” as protein (derived from the Greek word proteios, meaning “holding first place” or of the prime importance) in 1838. Proteins are indeed a group of cellular com ...
... not exist”. On suggestion of one of his friends, the famous Swedish chemist Berzelius, Mulder named this “most important of all substances” as protein (derived from the Greek word proteios, meaning “holding first place” or of the prime importance) in 1838. Proteins are indeed a group of cellular com ...
INTERMEDIARY METABOLISM
... The next step in the biosynthesis of pyrimidine nucleotides is the acquisition of a ribose-phosphate group. Orotate reacts with PRPP to form orotidine 5'monophosphate. This reaction is catalyzed by orotate phosphoribosyltransferase and driven forward by the hydrolysis of pyrophosphate release in thi ...
... The next step in the biosynthesis of pyrimidine nucleotides is the acquisition of a ribose-phosphate group. Orotate reacts with PRPP to form orotidine 5'monophosphate. This reaction is catalyzed by orotate phosphoribosyltransferase and driven forward by the hydrolysis of pyrophosphate release in thi ...
Development of the Ruminant Digestive Tract
... • Fat must be emulsified to a particle size less than 4 um with lecithin or glycerol monostearate • Vitamin E and/or antioxidants must be supplemented if unsaturated fatty acids present • Fat in replacers may reduce diarrhea – Fat reduces concentration of lactose and protein – Fat reduces rate of pa ...
... • Fat must be emulsified to a particle size less than 4 um with lecithin or glycerol monostearate • Vitamin E and/or antioxidants must be supplemented if unsaturated fatty acids present • Fat in replacers may reduce diarrhea – Fat reduces concentration of lactose and protein – Fat reduces rate of pa ...
1 - Free
... 12. write the name of two anaplerotic reactions. 13. what are the two most important products of the penthose phosphate pathway? What are these products used for? 14. write with structures the glugoneogenic reaction that is regulated by fructose 2,6bisphosphate. 15. list the enzymes that are involve ...
... 12. write the name of two anaplerotic reactions. 13. what are the two most important products of the penthose phosphate pathway? What are these products used for? 14. write with structures the glugoneogenic reaction that is regulated by fructose 2,6bisphosphate. 15. list the enzymes that are involve ...
Prediction of DNA-binding residues in proteins from amino acid
... 2007; Ho et al., 2007; Kuznetsov et al., 2006; Ofran et al., 2007; Wang and Brown, 2006a, b). In the present work, we aim to design optimal predictors for DNA-binding sites in proteins directly from amino acid sequences. In designing classifiers, the following points are taken into consideration: (i ...
... 2007; Ho et al., 2007; Kuznetsov et al., 2006; Ofran et al., 2007; Wang and Brown, 2006a, b). In the present work, we aim to design optimal predictors for DNA-binding sites in proteins directly from amino acid sequences. In designing classifiers, the following points are taken into consideration: (i ...
Amino Acids, Peptides, and Proteins
... H3NOCH2OCOOH with a net positive charge of 1.0. However, at pH 2.34, where there is an equal mixture of H3NOCH2OCOOH and H3NOCH2OCOO, the average or net charge on the population of glycine molecules is 0.5 (see the discussion on pp. 80–81). You can use the Henderson-Hasselbalch equation to calcul ...
... H3NOCH2OCOOH with a net positive charge of 1.0. However, at pH 2.34, where there is an equal mixture of H3NOCH2OCOOH and H3NOCH2OCOO, the average or net charge on the population of glycine molecules is 0.5 (see the discussion on pp. 80–81). You can use the Henderson-Hasselbalch equation to calcul ...
Oxidation of fatty acids in eukaryotes
... and adipose tissue [4]. Kinetic evidence has been obtained for both a saturable and nonsaturable uptake of fatty acids. The saturable uptake, which predominates at nanomolar concentrations of free fatty acids, is presumed to be carrier-mediated, whereas the non-saturable uptake, which is significant ...
... and adipose tissue [4]. Kinetic evidence has been obtained for both a saturable and nonsaturable uptake of fatty acids. The saturable uptake, which predominates at nanomolar concentrations of free fatty acids, is presumed to be carrier-mediated, whereas the non-saturable uptake, which is significant ...
Endelige eksamen 27008 MED svar
... transport is the only option. Q8 (1 point) Mark the statements below as either “True” or “False”. There may be more than one “True” statement. The mitochondrial ATP synthase: a. ...
... transport is the only option. Q8 (1 point) Mark the statements below as either “True” or “False”. There may be more than one “True” statement. The mitochondrial ATP synthase: a. ...
Lesson Overview
... be kept between 6.5 and 7.5 in order to maintain homeostasis. If the pH is lower or higher, it will affect the chemical reactions that take place within the cells. One of the ways that organisms control pH is through dissolved compounds called buffers, which are weak acids or bases that can react wi ...
... be kept between 6.5 and 7.5 in order to maintain homeostasis. If the pH is lower or higher, it will affect the chemical reactions that take place within the cells. One of the ways that organisms control pH is through dissolved compounds called buffers, which are weak acids or bases that can react wi ...
An hierarchical artificial neural network system for the classification
... application with a pre-processing stage, represented by an artificial neural network, which attempts to classify proteins into either membrane or non-membrane proteins. Several applications of neural networks to the prediction of transmembrane segments or secondary structure prediction can be found ...
... application with a pre-processing stage, represented by an artificial neural network, which attempts to classify proteins into either membrane or non-membrane proteins. Several applications of neural networks to the prediction of transmembrane segments or secondary structure prediction can be found ...
Purification and proteomic characterization of plastids from Brassica
... Plastids are functionally and structurally diverse organelles responsible for numerous biosynthetic reactions within the plant cell. Plastids from embryos have a range of properties depending upon the plant source but compared to other plastid types are poorly understood and therefore, we term them ...
... Plastids are functionally and structurally diverse organelles responsible for numerous biosynthetic reactions within the plant cell. Plastids from embryos have a range of properties depending upon the plant source but compared to other plastid types are poorly understood and therefore, we term them ...
Chapter 2
... The concentration of H+ or OH– expressed on the pH scale pH scale: 0–14 pH 7.0: H+ concentration = OH– concentration pH < 7.0 = more H+ (acid) ...
... The concentration of H+ or OH– expressed on the pH scale pH scale: 0–14 pH 7.0: H+ concentration = OH– concentration pH < 7.0 = more H+ (acid) ...
- Pacific Biomarkers
... effects through several G protein-coupled receptors. PYY is primarily released from endocrine cells of the distal digestive tract and plays an important role in regulating food intake and energy balance. PYY, which can be detected in blood, is a 36 amino acid peptide which exists in the 1-36 form an ...
... effects through several G protein-coupled receptors. PYY is primarily released from endocrine cells of the distal digestive tract and plays an important role in regulating food intake and energy balance. PYY, which can be detected in blood, is a 36 amino acid peptide which exists in the 1-36 form an ...
Chapter 6 Power Points
... bond breaking and bond forming The initial energy needed to start a chemical reaction is called the free energy of activation, or activation energy (EA) Activation energy is often supplied in the form of thermal energy that the reactant molecules absorb from their surroundings ...
... bond breaking and bond forming The initial energy needed to start a chemical reaction is called the free energy of activation, or activation energy (EA) Activation energy is often supplied in the form of thermal energy that the reactant molecules absorb from their surroundings ...
Metabolism
Metabolism (from Greek: μεταβολή metabolē, ""change"") is the set of life-sustaining chemical transformations within the cells of living organisms. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to their environments. The word metabolism can also refer to all chemical reactions that occur in living organisms, including digestion and the transport of substances into and between different cells, in which case the set of reactions within the cells is called intermediary metabolism or intermediate metabolism.Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter by way of cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. Usually, breaking down releases energy and building up consumes energy.The chemical reactions of metabolism are organized into metabolic pathways, in which one chemical is transformed through a series of steps into another chemical, by a sequence of enzymes. Enzymes are crucial to metabolism because they allow organisms to drive desirable reactions that require energy that will not occur by themselves, by coupling them to spontaneous reactions that release energy. Enzymes act as catalysts that allow the reactions to proceed more rapidly. Enzymes also allow the regulation of metabolic pathways in response to changes in the cell's environment or to signals from other cells.The metabolic system of a particular organism determines which substances it will find nutritious and which poisonous. For example, some prokaryotes use hydrogen sulfide as a nutrient, yet this gas is poisonous to animals. The speed of metabolism, the metabolic rate, influences how much food an organism will require, and also affects how it is able to obtain that food.A striking feature of metabolism is the similarity of the basic metabolic pathways and components between even vastly different species. For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. These striking similarities in metabolic pathways are likely due to their early appearance in evolutionary history, and their retention because of their efficacy.