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Origin and Distribution of Calvin Cycle Fructose and
Origin and Distribution of Calvin Cycle Fructose and

... algae" appear to have recruited their plastid-targeted FBP sequences via horizontal gene transfer (HGT) in a nonendosymbiotic context. The diatom sequences (pla1) have very long branches corresponding to an evolutionary rate roughly two times higher than their cytosolic counterpart (see Fig. 1). Lon ...
Updated slides on gene prediction
Updated slides on gene prediction

... Long vs. Short ORFs • Long open reading frames may be a gene – At random, we should expect one stop codon in every (64/3) ~= 21 codons – However, genes are usually much longer than this • A basic approach is to scan for ORFs whose length exceeds certain threshold – This is naïve because some genes ( ...
TEXT Definition Chromosomal alterations are variations from the
TEXT Definition Chromosomal alterations are variations from the

... may be true to very small deletions, particularly in haploid organisms, suggesting that genes are close together, and many of them have indispensable functions. However, in some genetically well-known species, notably Drosophila, use has been made of small deletions to map very small areas of chromo ...
Repetitive complete hydatidiform mole can be biparental in origin
Repetitive complete hydatidiform mole can be biparental in origin

... which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increased expression of paternally derived genes is provided by studies of the development of reconstituted mouse eggs. Wh ...
Surveying Saccharomyces Genomes to Identify Functional Elements
Surveying Saccharomyces Genomes to Identify Functional Elements

... possible to test the functionality of sequences with relatively simple and incisive experiments. The Saccharomyces genus is composed of three subgroups (Barnett 1992). The sensu stricto species are physiologically similar to S. cerevisiae, are capable of forming stable diploids with each other, and ...
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor

... is performed by a phosphorylation cascade that might modulate the activity of transcription factors regulating gene expression. The ethylene signal appears to be perceived by a family of ethylene receptors. Three members of the putative ethylene receptor gene family, ETR1, ERS (for ETHYLENE RESPONSE ...
Evolutionary approaches to autism
Evolutionary approaches to autism

... ABSTRACT: Autism is a highly heritable neurodevelopmental disorder, which greatly reduces reproductive success. The combination of high heritability and low reproductive success raises an evolutionary question: why was autism not eliminated by natural selection? We review different perspectives on t ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.

... the plasmid has integrated. In particular, one must be aware of the possibility that integration has taken place at the chromosomal locus of the selectable marker, as it also provides homology for recombination. Indeed, there are situations where this event is desired, and can be accomplished by cle ...
Genome Visualisation and Annotation Tools: Artemis and ACT
Genome Visualisation and Annotation Tools: Artemis and ACT

... This is the main sequence view panel. The central 2 grey lines represent the forward (top) and reverse (bottom) DNA strands. Above and below those are the 3 forward and 3 reverse reading frames. Stop codons are marked as black vertical bars. Genes and other features (eg. Pfam and Prosite matches) ar ...
Phytozome Tutorial from David Goodstein
Phytozome Tutorial from David Goodstein

... family size, which node it was found at (if you performed an “all nodes” search; otherwise  the node is simply the one shown in the “Search Node” field up above), an automatically  assigned defline for the family (or “Hypothetical gene (#XXX) if no name could be assigned),  and a “fingerprint” showi ...
DNA Sequencing (cont.) - A computational tour of the human genome
DNA Sequencing (cont.) - A computational tour of the human genome

... Normal flora: >200 species, >trillions of individuals Flora–disease, flora–non-optimal health associations Timeline: ...
Functional tests of enhancer conservation between
Functional tests of enhancer conservation between

... animal evolution, prior to the ‘Cambrian explosion’ around 530 million years ago (Morris, 2000). Expression patterns of enhancer elements have been described in both species; furthermore, in the worm, expression pattern resolution is possible at the single-cell level. If functional conservation of e ...
PowerPoint Presentation - Презентация PowerPoint
PowerPoint Presentation - Презентация PowerPoint

Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE
Lyons/Hewitt/Suchocki/Yeh, CONCEPTUAL INTEGRATED SCIENCE

... Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
Blue cone monochromacy: Causative mutations and associated
Blue cone monochromacy: Causative mutations and associated

... electropherograms, conferring different opsin spectral properties, present in exons 2 to 5. Coding sequence of L and M exons 1 and 6 were identical and could not be differentiated on an electropherogram; however, sequence upstream of the start codon of exon 1 contained nucleotide differences which f ...
Recombinant Materials Form
Recombinant Materials Form

... are constructed by joining nucleic acid molecules that can replicate in a living cell, or (ii) nucleic acid molecules that are chemically or by other means synthesized or amplified, including those that are chemically or otherwise modified but can base pair with naturally occurring nucleic acid mole ...
Chromosomal breakpoint positions suggest a direct role for radiation
Chromosomal breakpoint positions suggest a direct role for radiation

... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
RNA-Seq Analysis Practicals
RNA-Seq Analysis Practicals

... Common = 300/6 = 50% in both ...
Gene quantification using real-time quantitative PCR
Gene quantification using real-time quantitative PCR

... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
Annotation mapping functions
Annotation mapping functions

... Note: This can be resolved by using AnnotationDbi::mget(values, hom.Hs.inpBOSTA, ifnotfound=NA) which also returns a list object. We therefore present the function getOrthologs. It is faster and more powerful. Faster, as retrieving all mapped ids from bovine to human took approx. 11 minutes with the ...
N. crassa et al. However, despite the speed and
N. crassa et al. However, despite the speed and

... Increasing the efficiency of random isolates by using multiply marked testers: Rather that testing an unknown by crossing it to markers in the seven linkage groups one or two at a time, it is more efficient to use multiply marked strains. Testers have been developed that incorporate markers tagging ...
A pair of Sox: distinct and overlapping functions of
A pair of Sox: distinct and overlapping functions of

... like behaviors in neural plate cells and biases cells towards glial and melanocyte fates (Cheung and Briscoe, 2003). Sox9 also helps determine crest-derived chondrogenic lineages (MoriAkiyama et al., 2003; Spokony et al., 2002; Yan et al., 2002), and it functions in morphogenesis and differentiation ...
Isolation, Characterization, and Annotation: The Search for Novel
Isolation, Characterization, and Annotation: The Search for Novel

... proposed the use of bacteriophage to fight bacterial infections prior and pins which allows to the invention of antibiotics. In 1919, D’Herelle, a French scientist, the phage to attach to used phage to treat dysentery. Within a matter of years D’Herelle had a specific bacteria. commercial laboratory ...
Mary Ann Osley*, Alastair Fleming, and Cheng
Mary Ann Osley*, Alastair Fleming, and Cheng

... remove the ubiquitin moiety from histones and proteins that regulate the enzymatic machinery that promotes ubiquitylation. A review of these factors is presented in the following section and summarized in Table1. II.1 The ubiquitin conjugating pathway In this section, only a general overview of the ...
POSITION-EFFECT VARIEGATION AT SEVERAL
POSITION-EFFECT VARIEGATION AT SEVERAL

... that Dp" crosses produced flies which had, on the average, well over three fourths (4.24) of the area of the eyes normal for pigmentation while Dp" crosses produced flies with a n average of less than three fourths (2.74) of the eye showing normal pigmentation. For split variegation. Dpk gave, o n t ...
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Minimal genome

The concept of minimal genome assumes that genomes can be reduced to a bare minimum, given that they contain many non-essential genes of limited or situational importance to the organism. Therefore, if a collection of all the essential genes were put together, a minimum genome could be created artificially in a stable environment. By adding more genes, the creation of an organism of desired properties is possible. The concept of minimal genome arose from the observations that many genes do not appear to be necessary for survival. In order to create a new organism a scientist must determine the minimal set of genes required for metabolism and replication. This can be achieved by experimental and computational analysis of the biochemical pathways needed to carry out basic metabolism and reproduction. A good model for a minimal genome is Mycoplasma genitalium, the organism with the smallest known genome. Most genes that are used by this organism are usually considered essential for survival; based on this concept a minimal set of 256 genes has been proposed.
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