CBP/p300 in cell growth, transformation, and development Richard
... to participate in a variety of cellular functions (Fig. 1). ...
... to participate in a variety of cellular functions (Fig. 1). ...
Life and death of transcriptional co
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
... cytoplasmic protein aggregates or to deliver them for disposal p300 modification and metabolic stability in epigenetic regulathrough the alternative autophagosome pathway. Many nuclear tion, and shed molecular insights into the roles of cellular trafproteins including p300 are substrate of aggresome ...
Transcriptional regulation of mammalian autophagy at a glance
... referred to as p53) has been described to inhibit mTORC1 and thus to activate autophagy, several studies have shown that cytoplasmic p53 is a potent inhibitor of autophagy. The mechanisms for this inhibition are largely unknown (Green and Kroemer, 2009); however, post-transcriptional downregulation ...
... referred to as p53) has been described to inhibit mTORC1 and thus to activate autophagy, several studies have shown that cytoplasmic p53 is a potent inhibitor of autophagy. The mechanisms for this inhibition are largely unknown (Green and Kroemer, 2009); however, post-transcriptional downregulation ...
A tightly regulated and reversibly inducible siRNA expression
... be efficiently transcribed from either RNA polymerase (Pol) II- or Pol III-dependent promoters [16]. Currently, the Pol III-dependent RNase P RNA (H1) and U6 small nuclear RNA (U6) promoters from human and mouse have been used most frequently to drive the transient or constitutive expression of shRN ...
... be efficiently transcribed from either RNA polymerase (Pol) II- or Pol III-dependent promoters [16]. Currently, the Pol III-dependent RNase P RNA (H1) and U6 small nuclear RNA (U6) promoters from human and mouse have been used most frequently to drive the transient or constitutive expression of shRN ...
1 Introduction of autophagy
... by enhancing apoptosis at transcription level. A study indicated that p53 intensifies autophagy by acting on autophagy-related gene. Another study showed that it is in cytoplasm but not cell nucleus that p53 dampen-downs autophagy. • DAPK (death-associated protein kinase): we know that the combinati ...
... by enhancing apoptosis at transcription level. A study indicated that p53 intensifies autophagy by acting on autophagy-related gene. Another study showed that it is in cytoplasm but not cell nucleus that p53 dampen-downs autophagy. • DAPK (death-associated protein kinase): we know that the combinati ...
TP53 mutations occur in 15.7% of ALL and are associated with MYC
... The most frequently mutated gene in cancer is TP53, which is well characterized in other hematological malignancies, including acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL). However, the frequency and prognostic impact of TP53 mutations has only rarely been studied in ALL thus ...
... The most frequently mutated gene in cancer is TP53, which is well characterized in other hematological malignancies, including acute myeloid leukemia (AML) and chronic lymphocytic leukemia (CLL). However, the frequency and prognostic impact of TP53 mutations has only rarely been studied in ALL thus ...
The RING Domain of Mdm2 Can Inhibit Cell
... biquitin chains that are necessary for recognition by the proteasome (20). One possibility is that mono-ubiquitination of p53 is required to expose a nuclear export signal, and that p53 polyubiquitination and degradation then proceed in the cytoplasm (21–23). Because Mdm2 is a direct transcriptional ...
... biquitin chains that are necessary for recognition by the proteasome (20). One possibility is that mono-ubiquitination of p53 is required to expose a nuclear export signal, and that p53 polyubiquitination and degradation then proceed in the cytoplasm (21–23). Because Mdm2 is a direct transcriptional ...
Identification of the Factors Responsible for the Interaction of
... is a highly conserved, essential and abundantly available (available 1–2% of total cellular protein), homo dimeric molecular chaperone found in the eukaryotic cells [1, 2]. Hsp90 is found in two isoforms; Hsp90 and Hsp90 [3]. The Hsp90 has been found to be stress inducible and overexpressed in ma ...
... is a highly conserved, essential and abundantly available (available 1–2% of total cellular protein), homo dimeric molecular chaperone found in the eukaryotic cells [1, 2]. Hsp90 is found in two isoforms; Hsp90 and Hsp90 [3]. The Hsp90 has been found to be stress inducible and overexpressed in ma ...
Nuclear Factor-κB Modulates the p53 Response in Neurons
... exposure to the topoisomerase1 inhibitor, camptothecin. In this model, death is dependent on at least two proximal signals, the tumor suppressor p53 and the cell cycle-related cyclin-dependent kinases (CDKs) (Park et al., 1998). These signals work in concert to control Bax translocation and activati ...
... exposure to the topoisomerase1 inhibitor, camptothecin. In this model, death is dependent on at least two proximal signals, the tumor suppressor p53 and the cell cycle-related cyclin-dependent kinases (CDKs) (Park et al., 1998). These signals work in concert to control Bax translocation and activati ...
Mapping the function of the NS3 protein of African horsesickness
... family, has a ten-segment double-stranded (ds)RNA genome that is encapsidated within a single non-enveloped virus particle. In addition to seven structural proteins (VP1-VP7), four non-structural proteins (NS1, NS2 and NS3/NS3A) are synthesized in infected cells but their function in the viral life ...
... family, has a ten-segment double-stranded (ds)RNA genome that is encapsidated within a single non-enveloped virus particle. In addition to seven structural proteins (VP1-VP7), four non-structural proteins (NS1, NS2 and NS3/NS3A) are synthesized in infected cells but their function in the viral life ...
Integration of TP53, DREAM, MMB-FOXM1 and RB
... RB-E2F, DREAM and MMB-FOXM1 form distinct CC regulatory complexes, their target genes are often grouped together and termed ‘RB-E2F targets’ or ‘E2F-responsive genes’. In this case, ‘RB’ refers to all three pocket proteins, RB, p107 and p130, and ‘E2F’ refers to all E2F transcription factors E2F1-8 ...
... RB-E2F, DREAM and MMB-FOXM1 form distinct CC regulatory complexes, their target genes are often grouped together and termed ‘RB-E2F targets’ or ‘E2F-responsive genes’. In this case, ‘RB’ refers to all three pocket proteins, RB, p107 and p130, and ‘E2F’ refers to all E2F transcription factors E2F1-8 ...
The EMBO Journal (2002) 21, 1704–1712
... activity is inhibited by Mdm2, which binds directly to the transactivation domain of p53 and blocks its activity (Momand et al., 1998). In addition, Mdm2 also functions as an E3 ubiquitin ligase for p53 (Honda et al., 1997). Since Mdm2-induced ubiquitylation of p53 targets p53 for proteasome-depende ...
... activity is inhibited by Mdm2, which binds directly to the transactivation domain of p53 and blocks its activity (Momand et al., 1998). In addition, Mdm2 also functions as an E3 ubiquitin ligase for p53 (Honda et al., 1997). Since Mdm2-induced ubiquitylation of p53 targets p53 for proteasome-depende ...
Does the baculovirus sulfhydryl oxidase Ac92 associate with and
... Ac92 could have an inhibitory function. Prevent apoptosis Prevent cell cycle arrest ...
... Ac92 could have an inhibitory function. Prevent apoptosis Prevent cell cycle arrest ...
Construction and applications of yellow fever virus replicons
... coding sequence has been shown to be essential for autonomous replication. This sequence, which has been previously recognized as an RNA cyclization sequence (Hahn et al., 1987), is located within the coding sequence of the first 20 codons of the capsid protein. The importance of this region for gen ...
... coding sequence has been shown to be essential for autonomous replication. This sequence, which has been previously recognized as an RNA cyclization sequence (Hahn et al., 1987), is located within the coding sequence of the first 20 codons of the capsid protein. The importance of this region for gen ...
FGF1 inhibits p53-dependent apoptosis and cell cycle arrest via an
... confirm that FGF1 mRNA regulation was p53-dependent by inducing p53 activation in an independent manner, using etoposide in REtsAF cells. Etoposide, like temperature shift, induces the activation of p53, as confirmed by p21 and bax transactivation (Figure 1c and f), and leads to similar transient repr ...
... confirm that FGF1 mRNA regulation was p53-dependent by inducing p53 activation in an independent manner, using etoposide in REtsAF cells. Etoposide, like temperature shift, induces the activation of p53, as confirmed by p21 and bax transactivation (Figure 1c and f), and leads to similar transient repr ...
INPS: predicting the impact of non-synonymous variations on protein
... without relying on the structure. Here, we describe INPS, a novel approach for annotating the effect of non-synonymous mutations on the protein stability from its sequence. INPS is based on SVM regression and it is trained to predict the thermodynamic free energy change upon single-point variations ...
... without relying on the structure. Here, we describe INPS, a novel approach for annotating the effect of non-synonymous mutations on the protein stability from its sequence. INPS is based on SVM regression and it is trained to predict the thermodynamic free energy change upon single-point variations ...
Surgical Oncology
... Exist in all cells of the body, including reproductive cells The mutation can be passed from generation to generation. Also called germline mutations. Accounts for 5% to 10% of cancers ...
... Exist in all cells of the body, including reproductive cells The mutation can be passed from generation to generation. Also called germline mutations. Accounts for 5% to 10% of cancers ...
Recognition of Local DNA Structures by p53 Protein
... p53 is one of the most intensively studied tumor suppressor proteins and its regulation and relation to cancer has been reviewed extensively [1–3]. The reason for such interest is obvious; more than 50% of all human tumors contain Tp53 mutations and inactivation of this gene plays a critical role in ...
... p53 is one of the most intensively studied tumor suppressor proteins and its regulation and relation to cancer has been reviewed extensively [1–3]. The reason for such interest is obvious; more than 50% of all human tumors contain Tp53 mutations and inactivation of this gene plays a critical role in ...
Posttranslational Modification of MDM2
... mediates autoubiquitination as well as the ubiquitination of other substrates including p53 (18, 20, 21). Interestingly, MDM2 mediates multiple monoubiquitin attachment to p53 (22) while p300 mediates subsequent polyubiquitination (9). The E3 activity of MDM2 is dependent on its RING finger domain a ...
... mediates autoubiquitination as well as the ubiquitination of other substrates including p53 (18, 20, 21). Interestingly, MDM2 mediates multiple monoubiquitin attachment to p53 (22) while p300 mediates subsequent polyubiquitination (9). The E3 activity of MDM2 is dependent on its RING finger domain a ...
Characterization of the Determinants of NS2-3
... NS2-3 by just two amino acid exchanges. While the mechanism behind this gain of function remains elusive, the fact that it can be achieved by such minor changes is in line with the assumption that an ancestral virus already used this mechanism but lost it in the course of adapting to a new host/infe ...
... NS2-3 by just two amino acid exchanges. While the mechanism behind this gain of function remains elusive, the fact that it can be achieved by such minor changes is in line with the assumption that an ancestral virus already used this mechanism but lost it in the course of adapting to a new host/infe ...
RNA polymerase III component Rpc9 regulates
... Fig. 2. Lack of Rpc9 is responsible for the phenotype of mutant line 116 embryos. (A) Schematic of positional cloning. a-g indicate the locations of polymorphic primers distributed between 29,708,538 to 30,321,343 bp of chromosome 21 and the ratios of recombination out of total 2208 embryos in each ...
... Fig. 2. Lack of Rpc9 is responsible for the phenotype of mutant line 116 embryos. (A) Schematic of positional cloning. a-g indicate the locations of polymorphic primers distributed between 29,708,538 to 30,321,343 bp of chromosome 21 and the ratios of recombination out of total 2208 embryos in each ...
The nonhomologous end joining factor Artemis suppresses multi
... et al., 2005). The importance of NHEJ in genomic stability suggests that this pathway should suppress tumors in many somatic tissues, but roles for NHEJ in tumor suppression have primarily been characterized in B-lymphocytes. Loss of heterozygosity (LOH) is a common genetic aberration in both sporad ...
... et al., 2005). The importance of NHEJ in genomic stability suggests that this pathway should suppress tumors in many somatic tissues, but roles for NHEJ in tumor suppression have primarily been characterized in B-lymphocytes. Loss of heterozygosity (LOH) is a common genetic aberration in both sporad ...
Hepatitis C Virus: Genome Organization, Viral Proteins and
... E2 in the HCV open reading frame was predicted to be from amino acids 384 to 729 (16). It was reported that in an in vitro translation system, the full E2 coding region does not extend past amino acid 740 (46). More recent data suggested that the N terminal position of NS2 lies at about amino acid 8 ...
... E2 in the HCV open reading frame was predicted to be from amino acids 384 to 729 (16). It was reported that in an in vitro translation system, the full E2 coding region does not extend past amino acid 740 (46). More recent data suggested that the N terminal position of NS2 lies at about amino acid 8 ...
Gene Section VRK1 (Vaccinia-related kinase 1) Atlas of Genetics and Cytogenetics
... hypersensitive site located between VRK1 and BCL11B genes; but the structure, or expression, of VRK1 does not appear to be affected. In this translocation there is a dysregulation of TLX3 and NKX2-5 homeobox genes (both on chromosome 5). ...
... hypersensitive site located between VRK1 and BCL11B genes; but the structure, or expression, of VRK1 does not appear to be affected. In this translocation there is a dysregulation of TLX3 and NKX2-5 homeobox genes (both on chromosome 5). ...
P53
Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformation-related protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). This homolog (originally thought to be, and often spoken of as, a single protein) is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. As such, p53 has been described as ""the guardian of the genome"" because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene.The name p53 was given in 1979 describing the apparent molecular mass; SDS-PAGE analysis indicates that it is a 53-kilodalton (kDa) protein. In addition to the full length protein, the human TP53 gene encodes at least 15 protein isoforms, ranging in size from 3.5 to 53 kDa. All these p53 proteins are called the p53 isoforms.The International Cancer Genome Consortium has established that the TP53 gene is the most frequently mutated gene (>50%) in human cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation.TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome.