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Ch. 13.4: DNA Applications
Ch. 13.4: DNA Applications

... used to clone genes? Summarize the process. (Recombinant insulin plasmid) a. What is the role of restriction enzyme?  What natural role do they play in bacteria?  How are they used by humans?  What do the “sticky ends” produced by plasmids allow scientists to do? b. What is the role of ligase? 4. ...
Speciation - Deans Community High School
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Chapter 13: Genetic Engineering
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... • (almost) all inheritance is based on DNA: the sequence of ACGT nucleotides encodes all instructions needed to build and maintain an organism. • A chromosome is a single DNA molecule together with other molecules (proteins and RNA) needed to support and read the DNA. • A gene is a specific region o ...
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DNA FINGERPRINTING
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Guided Notes-Genetic Code

... What is a gene? How does a gene specify the production of a protein? How many bases are needed to specify an amino acid What is the three base code known as? How many codons are there? How many code for amino acids? There are 61 codons that code for amino acids but only 20 amino acids. Explain Give ...
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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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