Interphase
... There are three stages of interphase, with each phase ending when a cellular checkpoint checks the accuracy of the stage's completion before proceeding to the next. The stages of interphase are: • G1 (Growth 1), in which the cell grows and functions normally. During this time, much protein synthesis ...
... There are three stages of interphase, with each phase ending when a cellular checkpoint checks the accuracy of the stage's completion before proceeding to the next. The stages of interphase are: • G1 (Growth 1), in which the cell grows and functions normally. During this time, much protein synthesis ...
Candida albicans Iff11, a Secreted Protein Required for Cell Wall
... Preparation of protein extracts and Western blot analysis. Secreted protein fractions from cells grown in SC-U were filter sterilized (0.45-m pore diameter) and then concentrated, and the buffer was exchanged to phosphate-buffered saline (PBS) by ultrafiltration (Amicon Ultra-10-kDa Cutoff; Millipo ...
... Preparation of protein extracts and Western blot analysis. Secreted protein fractions from cells grown in SC-U were filter sterilized (0.45-m pore diameter) and then concentrated, and the buffer was exchanged to phosphate-buffered saline (PBS) by ultrafiltration (Amicon Ultra-10-kDa Cutoff; Millipo ...
Protein Synthesis, Cell Division and the Cell Cycle in
... Shift-up to rich medium at 24 "C When yeast cells of strain ~ 3 6 4 agrown in PMM medium (mass doubling time, 260 min) were shifted by dilution into YEPD medium, the rate of protein synthesis increased almost immediately to the new value (mass doubling time, 170min) (Fig. 1). The rate of cell divisi ...
... Shift-up to rich medium at 24 "C When yeast cells of strain ~ 3 6 4 agrown in PMM medium (mass doubling time, 260 min) were shifted by dilution into YEPD medium, the rate of protein synthesis increased almost immediately to the new value (mass doubling time, 170min) (Fig. 1). The rate of cell divisi ...
Alfy, a novel FYVE-domain-containing protein associated with
... it prone to recombination. We have therefore based all our immunofluorescence and electron microscopy (EM) experiments on endogenous Alfy detected using a polyclonal rabbit anti-Alfy antibody. pMAL-AlfyCT639 was generated by subcloning the Cterminal 1917 nt from Alfy into pMAL-c2 (New England Biolab ...
... it prone to recombination. We have therefore based all our immunofluorescence and electron microscopy (EM) experiments on endogenous Alfy detected using a polyclonal rabbit anti-Alfy antibody. pMAL-AlfyCT639 was generated by subcloning the Cterminal 1917 nt from Alfy into pMAL-c2 (New England Biolab ...
Protein Synthesis, Cell Division and the Cell Cycle in
... Shift-up to rich medium at 24 "C When yeast cells of strain ~ 3 6 4 agrown in PMM medium (mass doubling time, 260 min) were shifted by dilution into YEPD medium, the rate of protein synthesis increased almost immediately to the new value (mass doubling time, 170min) (Fig. 1). The rate of cell divisi ...
... Shift-up to rich medium at 24 "C When yeast cells of strain ~ 3 6 4 agrown in PMM medium (mass doubling time, 260 min) were shifted by dilution into YEPD medium, the rate of protein synthesis increased almost immediately to the new value (mass doubling time, 170min) (Fig. 1). The rate of cell divisi ...
Amino Acid Uptake for the Synthesis of Secretory Protein by the
... With the adveDt of radlotracen, studies with "C· and "H·labelled amino acids provided ovenmelming evidence that mlJk proteins are of matIlID!lJ'y gland origin. Of the lactoproteins 8)1J1thesi.zed, casein IC't'OIInts for 80 per ceat or the total proteins. Mammary secretory cells synthesize e.>selltia ...
... With the adveDt of radlotracen, studies with "C· and "H·labelled amino acids provided ovenmelming evidence that mlJk proteins are of matIlID!lJ'y gland origin. Of the lactoproteins 8)1J1thesi.zed, casein IC't'OIInts for 80 per ceat or the total proteins. Mammary secretory cells synthesize e.>selltia ...
Protists
... Absorb food from their surroundings. Some have cell walls, some do not. Have specialized ways to move from place to place. ...
... Absorb food from their surroundings. Some have cell walls, some do not. Have specialized ways to move from place to place. ...
EMBO Workshop on Cell Size Regulation
... Michael Hall – mTOR signaling in growth and metabolism Mike Fainzilber – Acting locally to grow globally – localized mechanisms in cell size regulation Short talk – Helena Soares – How does cell polarity affect cell size? Short talk – Shixuan Liu - Mechanisms coordinating cell size with cell ...
... Michael Hall – mTOR signaling in growth and metabolism Mike Fainzilber – Acting locally to grow globally – localized mechanisms in cell size regulation Short talk – Helena Soares – How does cell polarity affect cell size? Short talk – Shixuan Liu - Mechanisms coordinating cell size with cell ...
glossary/definition cytology and histology
... Pathology; from Greek pathos, suffering AND logos, to study. Meaning; the scientific study of the nature of disease and its causes, processes, development, and consequences. Phagocytosis; from Greek phago, to eat AND cyte , hollow vessel AND osis, process. Meaning; the engulfing of microorganisms or ...
... Pathology; from Greek pathos, suffering AND logos, to study. Meaning; the scientific study of the nature of disease and its causes, processes, development, and consequences. Phagocytosis; from Greek phago, to eat AND cyte , hollow vessel AND osis, process. Meaning; the engulfing of microorganisms or ...
The ongoing search for the molecular basis of plant osmosensing
... acid motif RGD (Canut et al., 1998). This has led to the hypothesis that focal adhesion–like complexes may exist in plants and serve to sense alterations in plasma membrane–cell wall attachments caused by dehydration. Although direct orthologues of mammalian proteins such as integrins have not been ...
... acid motif RGD (Canut et al., 1998). This has led to the hypothesis that focal adhesion–like complexes may exist in plants and serve to sense alterations in plasma membrane–cell wall attachments caused by dehydration. Although direct orthologues of mammalian proteins such as integrins have not been ...
Transport of protein kinase C α into the nucleus requires intact
... into intact fibroblasts. As Fig. 5A shows, TRITC-BSA remains completely cytosolic after microinjection. In contrast, the bright nuclear fluorescence in Fig. 5B indicates strong nuclear accumulation of NLS-TRITC-BSA in untreated cells. Furthermore, cells which were treated with cytochalasin B (Fig. 5 ...
... into intact fibroblasts. As Fig. 5A shows, TRITC-BSA remains completely cytosolic after microinjection. In contrast, the bright nuclear fluorescence in Fig. 5B indicates strong nuclear accumulation of NLS-TRITC-BSA in untreated cells. Furthermore, cells which were treated with cytochalasin B (Fig. 5 ...
DOC - National Center for Macromolecular Imaging
... different organisms. The daunting task of understanding the functions and regulation of these genes can be simplified by associating each of these genes with one of 200-300 core biological processes. Since most proteins function in association with other proteins in cells, one way to simplify the pr ...
... different organisms. The daunting task of understanding the functions and regulation of these genes can be simplified by associating each of these genes with one of 200-300 core biological processes. Since most proteins function in association with other proteins in cells, one way to simplify the pr ...
Staining of Blood parasites other than malaria parasites
... b. If microfilariae of Loa loa, follow steps iii, iv, v and vi because the sheath of Loa loa does not stain with Giemsa. For all other sheathed microfilariae, proceed only to step iv. since their sheaths stain with Giemsa.. c. Stain with a 1 in 10 dilution of Giemsa stain in pH 7.2 buffered water fo ...
... b. If microfilariae of Loa loa, follow steps iii, iv, v and vi because the sheath of Loa loa does not stain with Giemsa. For all other sheathed microfilariae, proceed only to step iv. since their sheaths stain with Giemsa.. c. Stain with a 1 in 10 dilution of Giemsa stain in pH 7.2 buffered water fo ...
Bacterial_Pathogenesis_-_Frank_Lam
... bloodstream via bacteremia and infect the meninges • Entry into the bloodstream can also ...
... bloodstream via bacteremia and infect the meninges • Entry into the bloodstream can also ...
Metabolic Pathways
... Catabolic Pathways • Catabolic pathways break down complex molecules into simpler ones. • This results in the release of energy. • Respiration is an example of a catabolic pathway as glucose is broken down to produce carbon dioxide, water and ATP. ...
... Catabolic Pathways • Catabolic pathways break down complex molecules into simpler ones. • This results in the release of energy. • Respiration is an example of a catabolic pathway as glucose is broken down to produce carbon dioxide, water and ATP. ...
Microbiology
... The technique developed by Gram is still commonly used today. It involves heat-fixing a smear of bacteria to a clean microscope slide and then flooding it with crystal violet. All bacteria take up this stain. The smear is washed with Gram’s iodine to fix the stain and then decolourised with alcohol or ...
... The technique developed by Gram is still commonly used today. It involves heat-fixing a smear of bacteria to a clean microscope slide and then flooding it with crystal violet. All bacteria take up this stain. The smear is washed with Gram’s iodine to fix the stain and then decolourised with alcohol or ...
Stomate cells
... When the plant is turgid (full of water) the guard cells are swollen and the stomate is open. This allows carbon dioxide to enter the leaf for photosynthesis, but it also allows water to leave the opening. Water leaving the leaf is called transpiration. During times of water stress, the guard cells ...
... When the plant is turgid (full of water) the guard cells are swollen and the stomate is open. This allows carbon dioxide to enter the leaf for photosynthesis, but it also allows water to leave the opening. Water leaving the leaf is called transpiration. During times of water stress, the guard cells ...
Columbus County Schools Science Curriculum Guide SUBJECT
... CCSS.ELA-Literacy.RST.6-8.9 Compare and contrast the information gained from experiments, simulations, video, or multimedia sources with that gained from reading a text on the same topic. ...
... CCSS.ELA-Literacy.RST.6-8.9 Compare and contrast the information gained from experiments, simulations, video, or multimedia sources with that gained from reading a text on the same topic. ...
Synthetic cell surface receptors for delivery of therapeutics and probes
... internalized by the transferrin receptor (TFR), entry of cholera toxin (CTX) and simian virus-40 (SV40) after binding to ganglioside GM1, and trafficking of glycosylphosphatidylinositolanchored proteins (GPI-AP). Ligand-bound LDLR and TFR undergo clathrin-mediated endocytosis, whereas binding of CTX ...
... internalized by the transferrin receptor (TFR), entry of cholera toxin (CTX) and simian virus-40 (SV40) after binding to ganglioside GM1, and trafficking of glycosylphosphatidylinositolanchored proteins (GPI-AP). Ligand-bound LDLR and TFR undergo clathrin-mediated endocytosis, whereas binding of CTX ...
Introduction to the cytoskeleton
... binding sites for the myosin heads. This is what actually allows the whole process with ATP to occur. Otherwise they troponin, which is some other type of protein complex, will be covering the binding sites from the myosin heads. When calcium is present it binds to the troponin and moves it out of ...
... binding sites for the myosin heads. This is what actually allows the whole process with ATP to occur. Otherwise they troponin, which is some other type of protein complex, will be covering the binding sites from the myosin heads. When calcium is present it binds to the troponin and moves it out of ...
changes in DNA AT14A mediates the cell wall–plasma membrane
... hasmechanism. a small domain has sequence similarities to integrins from animals. servetogether as a transepigenetic Here,that the effects of Cd treatment on the DNA methylation patten Integrins are examined with membrane between the extracellular matrix and the cytoskeleton, whichlevel playand crit ...
... hasmechanism. a small domain has sequence similarities to integrins from animals. servetogether as a transepigenetic Here,that the effects of Cd treatment on the DNA methylation patten Integrins are examined with membrane between the extracellular matrix and the cytoskeleton, whichlevel playand crit ...
Cytosol
The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.