Predicting functional linkages from gene fusions with
... often occur in the same cellular pathway (Marcotte et al 1999b; Yanai et al 2001). It is clear that not all fusion events are equally valuable for inferring functional linkages between genes. For example, certain ‘promiscuous domains’ (Marcotte et al 1999a), such as ATP binding cassettes and SH3 dom ...
... often occur in the same cellular pathway (Marcotte et al 1999b; Yanai et al 2001). It is clear that not all fusion events are equally valuable for inferring functional linkages between genes. For example, certain ‘promiscuous domains’ (Marcotte et al 1999a), such as ATP binding cassettes and SH3 dom ...
Comparative Analysis of Two C-Terminal Kinesin Motor
... the product of a separate gene. The divergent tail domains of these motors are predicted to harbor specific information that directs them to their correct intracellular targets. In order to define the sequences responsible for the differential localization of these two motors, GFP was fused to motor ...
... the product of a separate gene. The divergent tail domains of these motors are predicted to harbor specific information that directs them to their correct intracellular targets. In order to define the sequences responsible for the differential localization of these two motors, GFP was fused to motor ...
Overcoming anoikis - Journal of Cell Science
... In some situations, despite inappropriate integrin expression, cells can survive loss of adhesion by ‘ignoring’ signals that would normally lead to cell cycle arrest and anoikis. One way for detached tumor cells to bypass the requirement of integrin signaling for cell survival is to constitutively a ...
... In some situations, despite inappropriate integrin expression, cells can survive loss of adhesion by ‘ignoring’ signals that would normally lead to cell cycle arrest and anoikis. One way for detached tumor cells to bypass the requirement of integrin signaling for cell survival is to constitutively a ...
Ch. 5 - Macromolecules
... – Is a long molecule consisting of many similar building blocks called monomers – Specific monomers make up each ...
... – Is a long molecule consisting of many similar building blocks called monomers – Specific monomers make up each ...
Cell Respiration notes
... – It converts glycerol to G3P; fatty acids are broken into 2-carbon fragments that enter the Kreb’s as acetyl coA – A gram of fat yields more than twice as much ATP as a gram of starch. – Because so many calories are in each gram of fat, a person must expend a large amount of energy to burn fat stor ...
... – It converts glycerol to G3P; fatty acids are broken into 2-carbon fragments that enter the Kreb’s as acetyl coA – A gram of fat yields more than twice as much ATP as a gram of starch. – Because so many calories are in each gram of fat, a person must expend a large amount of energy to burn fat stor ...
Membrane Protein Expression in Cell
... few millilitres, short reaction times that usually finish within a day, high tolerance of the CF systems for a wide variety of substances like detergents or lipids and high efficiency in label incorporation combined with almost no background protein expression. In contrast to conventional in vivo ex ...
... few millilitres, short reaction times that usually finish within a day, high tolerance of the CF systems for a wide variety of substances like detergents or lipids and high efficiency in label incorporation combined with almost no background protein expression. In contrast to conventional in vivo ex ...
Identification of Hedgehog Pathway Components by RNAi in
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
... Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) an ...
Standard PDF - Wiley Online Library
... protein called EnvC, and there are two regulated autolysins, AmiA and AmiB (Yang et al., 2011). In both cases activation of the CW hydrolase(s) at the septum is needed to enable the separation of progeny cells after division, explaining at least in part the deleterious effects of inactivation of the ...
... protein called EnvC, and there are two regulated autolysins, AmiA and AmiB (Yang et al., 2011). In both cases activation of the CW hydrolase(s) at the septum is needed to enable the separation of progeny cells after division, explaining at least in part the deleterious effects of inactivation of the ...
2. Aim of the thesis
... identified Lymnaea nAChR subunits in Lymnaea stagnalis. Using real-time quantitative polymerase chain reaction (qPCR) we show that the LnAChR subunits are predominantly expressed in the CNS. In situ hybridization (ISH) on sections of the Lymnaea CNS demonstrates that the LnAChR subunits are express ...
... identified Lymnaea nAChR subunits in Lymnaea stagnalis. Using real-time quantitative polymerase chain reaction (qPCR) we show that the LnAChR subunits are predominantly expressed in the CNS. In situ hybridization (ISH) on sections of the Lymnaea CNS demonstrates that the LnAChR subunits are express ...
檔案下載 - 國立宜蘭大學生物技術與動物科學系
... – Iso-electric focusing used in QC to identify two similar proteins that are difficult to separate by any other means • Each protein has a specific number of charged amino acids on its surface in specific places • Creates a unique electric signature known as its iso-electric point (IEP) where charge ...
... – Iso-electric focusing used in QC to identify two similar proteins that are difficult to separate by any other means • Each protein has a specific number of charged amino acids on its surface in specific places • Creates a unique electric signature known as its iso-electric point (IEP) where charge ...
Phosphatidylserine, a death knell
... acts on the extracytosolic, external face of the plasma membrane to regulate intercellular interactions. Appreciation of this new role for phospholipids was galvanized by the demonstration that phosphatidylserine (PS) appears on the surface of apoptotic lymphocytes and contributes to their phagocyto ...
... acts on the extracytosolic, external face of the plasma membrane to regulate intercellular interactions. Appreciation of this new role for phospholipids was galvanized by the demonstration that phosphatidylserine (PS) appears on the surface of apoptotic lymphocytes and contributes to their phagocyto ...
Cell membranes
... normally non-covalentinteraction with integral membrane proteins or with lipid head groups ...
... normally non-covalentinteraction with integral membrane proteins or with lipid head groups ...
Overview of Metabolism - Chapter 4 - Formatted
... absorption now undergo in the cells of the body collectively constitute the phenomenon of metabolism. Every cell in a living organism works like an industrial organization. It is in a state of dynamic equilibrium, constantly taking in substances from its external environment, processing them, synthe ...
... absorption now undergo in the cells of the body collectively constitute the phenomenon of metabolism. Every cell in a living organism works like an industrial organization. It is in a state of dynamic equilibrium, constantly taking in substances from its external environment, processing them, synthe ...
The interaction between the Wnt –and Notch-pathways in
... This total down regulation of the Notch pathway upon Wnt deactivation shows a correlation between the two signaling pathways in colorectal cancer. We did not find any correlation the other way around, between Notch inhibition and Wnt pathway through β-catenin signalling. Using gamma-secretase inhibi ...
... This total down regulation of the Notch pathway upon Wnt deactivation shows a correlation between the two signaling pathways in colorectal cancer. We did not find any correlation the other way around, between Notch inhibition and Wnt pathway through β-catenin signalling. Using gamma-secretase inhibi ...
A Dictyostelium Mutant with Reduced Lysozyme Levels
... Endocytosis and Cell Growth—The uptake of particles and fluid phase was measured as described previously (34, 35). To determine the capacity of cells to degrade bacteria in vivo, we used the assay established by Maselli et al. (36), except that we used E. coli expressing the green fluorescent protei ...
... Endocytosis and Cell Growth—The uptake of particles and fluid phase was measured as described previously (34, 35). To determine the capacity of cells to degrade bacteria in vivo, we used the assay established by Maselli et al. (36), except that we used E. coli expressing the green fluorescent protei ...
Final Report SID5
... Seven genes form S. uberis considered to encode proteases that were likely to be exported outside the cell were identified from the recently completed and annotated genome sequence of S. uberis. These genes were cloned in such a manner that the encoded protein was produced with an additional six ami ...
... Seven genes form S. uberis considered to encode proteases that were likely to be exported outside the cell were identified from the recently completed and annotated genome sequence of S. uberis. These genes were cloned in such a manner that the encoded protein was produced with an additional six ami ...
3.2 Cell Organelles KEY CONCEPT Eukaryotic cells share many similarities.
... 3.2 Cell Organelles Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... 3.2 Cell Organelles Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
A family of abundant plasma membrane
... coincident migration during isoelectric focussing of hydroxyproline-rich membrane components and components with affinity for ~-galactosyl Yariv reagent (Samson et al., 1983). However, since Yariv reagents do not interact with AGPs specifically (Jermyn, 1978), and hydroxyproline is the principal ami ...
... coincident migration during isoelectric focussing of hydroxyproline-rich membrane components and components with affinity for ~-galactosyl Yariv reagent (Samson et al., 1983). However, since Yariv reagents do not interact with AGPs specifically (Jermyn, 1978), and hydroxyproline is the principal ami ...
3.2 Cell Organelles Several organelles are involved in making and
... 3.2 Cell Organelles Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
... 3.2 Cell Organelles Several organelles are involved in making and processing proteins. • The nucleus stores genetic information. • Many processes occur in the endoplasmic reticulum. • There are two types of endoplasmic reticulum. – rough endoplasmic reticulum – smooth endoplasmic reticulum ...
Amniotic membrane modulates innate immune response inhibiting
... b, and by the other hand activate the nuclear factor-kB (NFkB) favoring the production of proinflammatory cytokines (Kawai et al., 2005; Hirata et al., 2007; Kanneganti, 2010). In this context, it has been described that NFkB is an important molecule in cell activation process throughout maintaining ...
... b, and by the other hand activate the nuclear factor-kB (NFkB) favoring the production of proinflammatory cytokines (Kawai et al., 2005; Hirata et al., 2007; Kanneganti, 2010). In this context, it has been described that NFkB is an important molecule in cell activation process throughout maintaining ...
The src
... FAK is recruited by integrins to the membrane and is autophosphorylated - Src binds to phophorylated FAK - Src changes conformation and becomes active - Src further phosphorylates FAK - Src-FAK phosphorylate target proteins ...
... FAK is recruited by integrins to the membrane and is autophosphorylated - Src binds to phophorylated FAK - Src changes conformation and becomes active - Src further phosphorylates FAK - Src-FAK phosphorylate target proteins ...
Bioinformatics and Functional Genomics, Chapter 8, Part 1
... Physical properties of proteins Many websites are available for the analysis of individual proteins. ExPASy and ISREC are two excellent resources. The accuracy of these programs is variable. Predictions based on primary amino acid sequence (such as molecular weight prediction) are likely to be more ...
... Physical properties of proteins Many websites are available for the analysis of individual proteins. ExPASy and ISREC are two excellent resources. The accuracy of these programs is variable. Predictions based on primary amino acid sequence (such as molecular weight prediction) are likely to be more ...
The functional cobalamin (vitamin B12)–intrinsic factor receptor is a
... site (CUB domains 5-8).5,6 Although cubilin has no apparent transmembrane segment or cytoplasmic tail, several studies have shown that binding of IF-cobalamin to cubilin leads to endocytosis of the ligand and recycling of the receptor.2,3 Besides expression and function in the intestine, cubilin has ...
... site (CUB domains 5-8).5,6 Although cubilin has no apparent transmembrane segment or cytoplasmic tail, several studies have shown that binding of IF-cobalamin to cubilin leads to endocytosis of the ligand and recycling of the receptor.2,3 Besides expression and function in the intestine, cubilin has ...
- de Duve Institute
... The aim of our research is to understand the molecular mechanisms underlying a variety of disorders of the cardiovascular and skeletal systems, as well as certain cancers. We are especially interested in evaluating the contribution of genetic variation to human disease. The bases of many disorders r ...
... The aim of our research is to understand the molecular mechanisms underlying a variety of disorders of the cardiovascular and skeletal systems, as well as certain cancers. We are especially interested in evaluating the contribution of genetic variation to human disease. The bases of many disorders r ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.