Adrenocortical Hyperfunction (Hyperadrenalism)
... Primary adrenal neoplasms, such as adrenal adenoma (~10%) and carcinoma (~5%) are the most common underlying causes for ACTH-independent Cushing syndrome. The biochemical sine qua non of ACTHindependent Cushing syndrome is elevated serum levels of cortisol with low levels of ACTH. Cortical carcinoma ...
... Primary adrenal neoplasms, such as adrenal adenoma (~10%) and carcinoma (~5%) are the most common underlying causes for ACTH-independent Cushing syndrome. The biochemical sine qua non of ACTHindependent Cushing syndrome is elevated serum levels of cortisol with low levels of ACTH. Cortical carcinoma ...
The Protoplast: Plasma Membrane, Nucleus, and Cytoplasmic
... The word cell, meaning “little room,” was introduced by Robert Hooke in the seventeenth century to describe the small cavities separated by cell walls in cork tissue. Later Hooke recognized that living cells in other plant tissues were fi lled with “juices.” Eventually the contents of cells were int ...
... The word cell, meaning “little room,” was introduced by Robert Hooke in the seventeenth century to describe the small cavities separated by cell walls in cork tissue. Later Hooke recognized that living cells in other plant tissues were fi lled with “juices.” Eventually the contents of cells were int ...
Poster
... 100% of the EPSC amplitude because there is no 2-AG present. At time 0, the electrical pulses were vastly increased for a few milliseconds, triggering 2-AG synthesis. The presence of 2-AG decreased the release of glutamate reflected in a decrease in EPSC amplitude. The control line returned to 100% ...
... 100% of the EPSC amplitude because there is no 2-AG present. At time 0, the electrical pulses were vastly increased for a few milliseconds, triggering 2-AG synthesis. The presence of 2-AG decreased the release of glutamate reflected in a decrease in EPSC amplitude. The control line returned to 100% ...
Characterization of a Negative Thyroid Hormone Response Element
... XbaIII and SacII/XbaIII, respectively. DNA was labeled as previously described (13). Briefly, 50 ng of either annealed oligonucleotide or DNA fragments produced by restriction enzyme digestion were incubated with 6 U Klenow fragment in the presence of 1 mm deoxy (d)-ATP, dTTP, and dGTP and 50 mCi [3 ...
... XbaIII and SacII/XbaIII, respectively. DNA was labeled as previously described (13). Briefly, 50 ng of either annealed oligonucleotide or DNA fragments produced by restriction enzyme digestion were incubated with 6 U Klenow fragment in the presence of 1 mm deoxy (d)-ATP, dTTP, and dGTP and 50 mCi [3 ...
SCENTS AND SENSIBILITY: A MOLECULAR LOGIC OF OLFACTORY PERCEPTION
... There are many ways for organisms to probe the external world. Some smell it, others listen to it, many see it. Each species therefore lives in its own unique sensory world of which other species may be partially or totally unaware. A whole series of specific devices alien to human perception have e ...
... There are many ways for organisms to probe the external world. Some smell it, others listen to it, many see it. Each species therefore lives in its own unique sensory world of which other species may be partially or totally unaware. A whole series of specific devices alien to human perception have e ...
NOBEL LECTURE: Yeast and Cancer
... the primary defect. After the primary defect, other cell cycle events would occur or not depending upon the particular mutant. Eventually, the cell would arrest development and generate a terminal phenotype, depending on which events occurred and which did not. Mutants were found with primary defect ...
... the primary defect. After the primary defect, other cell cycle events would occur or not depending upon the particular mutant. Eventually, the cell would arrest development and generate a terminal phenotype, depending on which events occurred and which did not. Mutants were found with primary defect ...
Protein Secondary Structure
... What terminates an -helix? Statistically, a very high percentage (~60%) of helices are terminated by a single amino acid, Proline: ...
... What terminates an -helix? Statistically, a very high percentage (~60%) of helices are terminated by a single amino acid, Proline: ...
Clathrinmediated transport: assembly required
... 100-fold. The C-terminal region of dynamin contains a proline-rich region that interacts with the Src homology 3 domains of several proteins, such as amphiphysin, endophilin and actin-binding proteins. J. Hinshaw (Bethesda, MD, USA) reported on the conformational changes she could observe in dynamin ...
... 100-fold. The C-terminal region of dynamin contains a proline-rich region that interacts with the Src homology 3 domains of several proteins, such as amphiphysin, endophilin and actin-binding proteins. J. Hinshaw (Bethesda, MD, USA) reported on the conformational changes she could observe in dynamin ...
(Heterobasidion annosum) in
... a pathogen attack, though well studied in crop plants, are poorly understood in conifers. To analyze changes in gene transcript abundance in Pinus sylvestris L. root tissues infected by Heterobasidion annosum (Fr.) Bref. s.l., a cDNA microarray containing 2109 ESTs from P. taeda L. was used. Mixed m ...
... a pathogen attack, though well studied in crop plants, are poorly understood in conifers. To analyze changes in gene transcript abundance in Pinus sylvestris L. root tissues infected by Heterobasidion annosum (Fr.) Bref. s.l., a cDNA microarray containing 2109 ESTs from P. taeda L. was used. Mixed m ...
A mutation in the nucleoporin-107 gene causes XX gonadal
... Downloaded from http://www.jci.org on November 25, 2015. http://dx.doi.org/10.1172/JCI83553 ...
... Downloaded from http://www.jci.org on November 25, 2015. http://dx.doi.org/10.1172/JCI83553 ...
12.1 Mechanisms regulating enzyme synthesis 12.1.2.2 Enzyme
... The regulatory gene (lacI) next to the 5’ end of the operon is expressed constitutively with its own promoter. In the absence of the inducer, the LacI protein binds the operator region of the lac operon, inhibiting RNA polymerase from binding the promoter region. When the inducer (lactose or I ...
... The regulatory gene (lacI) next to the 5’ end of the operon is expressed constitutively with its own promoter. In the absence of the inducer, the LacI protein binds the operator region of the lac operon, inhibiting RNA polymerase from binding the promoter region. When the inducer (lactose or I ...
Genetic Techniques for Biological Research Case study II
... The secretory pathway can be viewed as a substrate-dependent pathway. Genetic analysis to date has not identified negative regulators. The secreted protein is the most upstream substrate. Thisis then acted on by a series of Sec proteins that act on the target protein in a stepwise fashion to translo ...
... The secretory pathway can be viewed as a substrate-dependent pathway. Genetic analysis to date has not identified negative regulators. The secreted protein is the most upstream substrate. Thisis then acted on by a series of Sec proteins that act on the target protein in a stepwise fashion to translo ...
Agonism with the omega-3 fatty acids α-linolenic acid
... quences (NCBI ID: BC101175 and NM_181745, respectively) to predict intra- and extra- cellular domains as well as membranespanning regions and their orientations. Protein sequences were aligned and examined using Vector NTI 10.0 software (Invitrogen). 2.4. Immunoprecipitation and immunoblotting Forty ...
... quences (NCBI ID: BC101175 and NM_181745, respectively) to predict intra- and extra- cellular domains as well as membranespanning regions and their orientations. Protein sequences were aligned and examined using Vector NTI 10.0 software (Invitrogen). 2.4. Immunoprecipitation and immunoblotting Forty ...
Chemokine-Induced Migration Inhibits Integrin
... time to time creates long “dendrites” (Fig. 2B and Ref. (17). Strikingly, when A39R was added at the beginning of the culture together with LPS, it completely prevented the formation of membrane processes by DCs (Fig. 2C) as well as their adhesion to plastic. This effect was dependent on plexin C1 a ...
... time to time creates long “dendrites” (Fig. 2B and Ref. (17). Strikingly, when A39R was added at the beginning of the culture together with LPS, it completely prevented the formation of membrane processes by DCs (Fig. 2C) as well as their adhesion to plastic. This effect was dependent on plexin C1 a ...
PROTEIN ANALYSIS - Farmasi Carbon 2012
... BICINCHONIC ACID (BCA) METHOD Advantages - as sensitive as Lowry but simpler - reagent more stable than Lowry Disadvantages - color not stable with time precise timing - reducing sugars interfere more than Lowry - color variations between proteins occur absorbance vs concentration not absolutely ...
... BICINCHONIC ACID (BCA) METHOD Advantages - as sensitive as Lowry but simpler - reagent more stable than Lowry Disadvantages - color not stable with time precise timing - reducing sugars interfere more than Lowry - color variations between proteins occur absorbance vs concentration not absolutely ...
Lesson Overview - Diman Regional
... Hormones – chemical messengers of the endocrine system, created by endocrine glands, bind to receptors of target cell, SLOWER ...
... Hormones – chemical messengers of the endocrine system, created by endocrine glands, bind to receptors of target cell, SLOWER ...
Domain conservation in several volvocalean cell wall - UvA-DARE
... contain (SerPro)x epitopes, affinity-purified antibodies generated against a (SerPro)10 oligopeptide (c~-(SerPro)10), [57]) were used to immunoprecipitate the in vitro translation products of poly(A) + RNA samples from three different stages of the life cycle in which (based on prior studies) cell w ...
... contain (SerPro)x epitopes, affinity-purified antibodies generated against a (SerPro)10 oligopeptide (c~-(SerPro)10), [57]) were used to immunoprecipitate the in vitro translation products of poly(A) + RNA samples from three different stages of the life cycle in which (based on prior studies) cell w ...
Use of infrared and visible light radiation as modulator of protein
... fifteen proto-oncogene proteins, that characterize their common biological activity, i.e. the ability to promote uncontrolled cell proliferation, in case of the oncogene proteins, and normal cell growth for proto-oncogenes [10]. This study emphasizes the de novo design of peptide analogues only on t ...
... fifteen proto-oncogene proteins, that characterize their common biological activity, i.e. the ability to promote uncontrolled cell proliferation, in case of the oncogene proteins, and normal cell growth for proto-oncogenes [10]. This study emphasizes the de novo design of peptide analogues only on t ...
Lab 14 Review Name: Osmosis Instructions: Log in to www
... 2. Choose one a measure of how much a given substance is mixed with another substance. 3. Choose one The spontaneous net movement of particles from area of high concentration to an area of low concentration. 4. Choose one a state of balance in which there is little or no total change. When particles ...
... 2. Choose one a measure of how much a given substance is mixed with another substance. 3. Choose one The spontaneous net movement of particles from area of high concentration to an area of low concentration. 4. Choose one a state of balance in which there is little or no total change. When particles ...
Protein sensing in living cells by molecular rotor
... from CHO cells, which are MGMT-decient, the other three types of cell lines have been known to express substantial amounts of MGMT.33,37 Live-cell images were taken immediately without any washout process aer 1 mM BG-CCVJ was incubated with the cells for 90 minutes (Fig. S6†). A strong uorescence ...
... from CHO cells, which are MGMT-decient, the other three types of cell lines have been known to express substantial amounts of MGMT.33,37 Live-cell images were taken immediately without any washout process aer 1 mM BG-CCVJ was incubated with the cells for 90 minutes (Fig. S6†). A strong uorescence ...
uric acid stimulates vascular smooth muscle cell proliferation by
... proliferation in vitro. However, the related intracellular mechanisms of uric acids in VSMCs remain to be determined. Cultured rat aortic SMCs were stimulated with uric acid, [3H]thymidine incorporation and the endothelin-1 (ET-1) gene expression was examined. Antioxidants pretreatment on uric acid- ...
... proliferation in vitro. However, the related intracellular mechanisms of uric acids in VSMCs remain to be determined. Cultured rat aortic SMCs were stimulated with uric acid, [3H]thymidine incorporation and the endothelin-1 (ET-1) gene expression was examined. Antioxidants pretreatment on uric acid- ...
Intracellular catalysis of disulfide bond formation by the human
... The predicted coding sequence for hQSOX1a contains a putative N-terminal signal peptide, a conserved N-glycosylation site and a C-terminal transmembrane domain [5]. To investigate whether the protein is indeed glycosylated and a transmembrane protein we performed in vitro translation of an RNA trans ...
... The predicted coding sequence for hQSOX1a contains a putative N-terminal signal peptide, a conserved N-glycosylation site and a C-terminal transmembrane domain [5]. To investigate whether the protein is indeed glycosylated and a transmembrane protein we performed in vitro translation of an RNA trans ...
isolation and characterization of a cell wall
... mutant allele. All strains were maintained as vegetative stocks on solid HS medium under continuous cool white fluorescent illumination (80 mmol photons m 2 s 1) at 201 C. Mutagenesis. Vegetative cells of UTEX 220 were suspended in 1 mL sterile HS medium (Sueoka 1960) to a final cell density ...
... mutant allele. All strains were maintained as vegetative stocks on solid HS medium under continuous cool white fluorescent illumination (80 mmol photons m 2 s 1) at 201 C. Mutagenesis. Vegetative cells of UTEX 220 were suspended in 1 mL sterile HS medium (Sueoka 1960) to a final cell density ...
The ~53 Proto-Oncogene Can Act as a Suppressor of Transformation
... derived p53 cDNAs and of at least one ~53 genomic clone, all of which possess mutations that activate the ~53 protein for cooperation with ras (Finlay et al., 1988; Eliyahu et al., 1988; Hinds et al., 1989). The mutations (the result of one or two single nucleotide changes) are localized between ami ...
... derived p53 cDNAs and of at least one ~53 genomic clone, all of which possess mutations that activate the ~53 protein for cooperation with ras (Finlay et al., 1988; Eliyahu et al., 1988; Hinds et al., 1989). The mutations (the result of one or two single nucleotide changes) are localized between ami ...
Biocatalytic Synthesis of Polymers of Precisely Defined Structures
... more complex globular counterparts. In addition, these polypeptides also adopt regular and persistent secondary structures in both the solution and solid states, consistent with the geometrical requirements of their oligopeptide repeats. By utilizing the principles of protein structure and the conce ...
... more complex globular counterparts. In addition, these polypeptides also adopt regular and persistent secondary structures in both the solution and solid states, consistent with the geometrical requirements of their oligopeptide repeats. By utilizing the principles of protein structure and the conce ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.