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Histological and ultrastructural analysis of the
Histological and ultrastructural analysis of the

... where the globular, heart and torpedo shape are observed. Somatic callus may exhibit morphological characteristics that affect formation, development and germination of embryos. Different morphogenetic expressions reflect the nature and degree of tissues differentiation (Stein et al., 2010) and thus ...
The Expression of Slow Myosin During Mammalian Somitogenesis
The Expression of Slow Myosin During Mammalian Somitogenesis

... Starting at day 13 of fetal development, the results obtained with the double labeling became different. Fig. 2, C and D, shows a culture of myogenic cells isolated from the limb of a 13-d-old fetus. Many thin elongated myotubes stain brightly with the anti-common (Fig. 2 C) but poorly with the anti ...
Introducing Antisense Oligonucleotides into Cells
Introducing Antisense Oligonucleotides into Cells

...  Proteins derived from the coat of Sendai viruses are known to promote fusion of lipid bilayers. In one series of experiments, oligonucleotides were packaged in liposomes complexed with coat proteins derived from the hemagglutinating virus of Japan (HVJ, a Sendai family virus) and were infused into ...
Is the shoot a root with a view? Philip N Benfey
Is the shoot a root with a view? Philip N Benfey

... developmental function in shoot and root should not come as a surprise to plant evolutionary biologists for whom it has always appeared likely that the root meristem evolved from an ancestral shoot. Developmental geneticists, on the other hand, have generally separated the plant into root and shoot ...
mbn cells results in dysregulation of Fer1HCH expression
mbn cells results in dysregulation of Fer1HCH expression

... l(2)mbn cells were harvested by scraping. Total RNA was extracted using TRIzol® reagent (Invitrogen), treated with DNase I (Ambion) and re-extracted with an equal volume of watersaturated phenol/chloroform (1:1, v/v) (Q-Biogene) followed by precipitation with 50 % isopropanol. The RNA pellets were r ...
Full Text  - Plant and Cell Physiology
Full Text - Plant and Cell Physiology

... Aurora A, Aurora B–INCENP, Nek2, Bub1, Plk1 and Mps1. To verify that 5-ITu is a valid inhibitor for plant Haspin, we conducted both in vitro and in vivo assays. The in vitro kinase assay was performed using purified glutathione S-transferease (GST)–AtHaspin and GST–AtHaspin-KD (kinase-dead) with or ...
Human embryonic stem cells express a unique set
Human embryonic stem cells express a unique set

... Human embryonic stem (hES) cells are pluripotent cell lines established from the explanted inner cell mass of human blastocysts. Despite their importance for human embryology and regenerative medicine, studies on hES cells, unlike those on mouse ES (mES) cells, have been hampered by difficulties in ...
Innate Immunity in Lophotrochozoans: The Annelids
Innate Immunity in Lophotrochozoans: The Annelids

... pattern recognition receptors (PRR), phagocytic cells, proteolytic cascades and peptides/proteins with antimicrobial properties. Each element of these events has been well studied in vertebrates and in some invertebrates such as annelids. From these different researches, it appears that mammalian in ...
Listeria Nontoxic radioactive is a highly effective therapy against metastatic pancreatic cancer
Listeria Nontoxic radioactive is a highly effective therapy against metastatic pancreatic cancer

... with advanced-stage disease (1–3), emphasizing the need for new alternative therapies for metastatic pancreatic cancer. For any anticancer approach to be effective, it needs to target metastases and/or remaining tumor cells after primary therapeutic intervention. Indeed, in most cases, cancer therap ...
THE ROLE OF MHC GENE PRODUCTS IN IMMUNE REGULATION AND
THE ROLE OF MHC GENE PRODUCTS IN IMMUNE REGULATION AND

... The study or Ir gene function contributed to our understanding of the intricate regulatory mechanisms evolved by T cells and macrophages to regulate specific immune responses. Experiments were initially designed to identify the cells of the immune system in which Ir genes are expressed and the natur ...
Question paper - Unit F222/01 - Growth, development and
Question paper - Unit F222/01 - Growth, development and

... Professor Mark Cline’s study of the effects of soy food on the development of breast cancer and uterine cancer in monkeys. Suggest why the findings of each of these studies cannot be applied to the wider human ...
ZEISS Microscopy Labs AutoLPC from glass slides results in good
ZEISS Microscopy Labs AutoLPC from glass slides results in good

... gene expression shall be analyzed in specific cell types and structures within tissue samples. Due to easier handling and faster work as well as better documentation possibilities a MembraneSlide is normally the recommended LCM-basis for any newly prepared sections especially when later RNA analyses ...
Cytoskeletal Changes of Mesenchymal Stem Cells During
Cytoskeletal Changes of Mesenchymal Stem Cells During

... Tissue Engineering ...
Logic of Definitions
Logic of Definitions

... (part of or deriving from an organism) that has as its parts a maximally connected cell compartment surrounded by a plasma membrane. (From FMA via CARO) • Comment: The definition of cell is intended to represent all cells, and thus a cell is defined as a material entity and not an anatomical structu ...
- ISpatula
- ISpatula

... adding coconut milk and they found that they can enhance the tobacco cells to divide by adding DNA samples. Both of them have modified forms of adenine: a component of nucleic acids. *They named as this because they stimulate cytokinesis or cell division. *The most common natural cytokine (zeatin) n ...
Bio 103 Lecture - Origin and Evolution of Micr
Bio 103 Lecture - Origin and Evolution of Micr

... • What were the characteristics of the atmosphere of early earth? • How much oxygen was present in the early earth's atmosphere, how much is present today and to what activity is the difference attributed? • What was the temperature (general) of the early earth, what caused that temperature, what wa ...
Title of the Project Cellular and molecular mechanisms of ozone
Title of the Project Cellular and molecular mechanisms of ozone

... The use of in vitro systems will ensure controlled and easily reproducible experimental conditions. hADAS are mesenchymal stem cells present in the stromal-vascular fraction of fat tissue; they have the capability to differentiate in vitro into meso-, ecto- and endodermal cell lineages and can also ...
ANTIBACTERIAL ACTIVITY OF HUMAN NATURAL KILLER CELLS
ANTIBACTERIAL ACTIVITY OF HUMAN NATURAL KILLER CELLS

... Effector cells were prepared and incubated in a serum-free, proteindefined media HB104 (DuPont Co., Wilmington, DE) . In a few experiments, cells were incubated in RPMI 1640, (Gibco Laboratories, Grand Island, NY) supplemented with 10% heat-inactivated human AB serum (Irvine Scientific, Irvine, CA) ...
Disruption of the long-range GPIIIa Cys5
Disruption of the long-range GPIIIa Cys5

... adhesive receptors capable of mediating cell-cell and cellextracellular matrix (ECM) interactions,1 are heterodimers consisting of a 120- to 180-kDa ␣ subunits noncovalently associated with a 90- to 110-kDa ␤ subunits. To date, 19 ␣ and 8 ␤ subunits have been characterized, and 24 members of this he ...
Differential Localization of Carbohydrate Epitopes in Plant Cell
Differential Localization of Carbohydrate Epitopes in Plant Cell

... between the Universities of Konstanz and Torino occurred in the frame of COST Action 821. Work on monoclonal antibodies against plant cell wall polysaccharides at the Complex Carbohydrate Research Center, University of Georgia, is supported by a grant from the U.S. Department of Energy (DE-FG05-93ER ...
Fluid-Phase Endocytosis in Plant Cells
Fluid-Phase Endocytosis in Plant Cells

... have been identified in animal systems, some of which are just beginning to be uncovered in plant cells. By definition and operational design, all types of endocytosis (whether clathrin-mediated, caveolin-mediated, lipid raft-dependent, flotillin-dependent, and micro- or macropinocytosis; Moscatelli ...
Name
Name

... ____________40. Slower to contract, but may retain contraction longer ...
Microfilaments Intermediate filaments
Microfilaments Intermediate filaments

... © 2014 Pearson Education, Inc. ...
Regulation of human embryonic stem cell differentiation by BMP
Regulation of human embryonic stem cell differentiation by BMP

... noggin were apparent using either lot of feeder cells at the density normally employed for ES cell maintenance. Human EC cell differentiation was induced by all-trans-retinoic acid or BMP-2 treatment as described elsewhere (Pera and Herszfeld, 1998; Roach et al., 1994). Treatment of ES cells with no ...
AGI June 41/6 - American Journal of Physiology
AGI June 41/6 - American Journal of Physiology

... protein coat can exclude particles in the size range of most viruses from contact with membrane glycolipids in vivo. This is important because it has been shown that HIV-1 (120 nm in diameter) attaches to intestinal cells in culture via galactosylceramide (9, 12). HIV can adhere to M cells in rabbit ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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