Architectural remodeling of the tonoplast during fluid
... or resting stage, red beet hypocotyl storage cells contained predominantly one large central vacuole (Fig. 2A and B). However, after tissue incubation in 200 mM sucrose for 24 h, a series of vacuole-derived organelles become evident (Fig. 2C and D), a condition scarcely observed when betaine was use ...
... or resting stage, red beet hypocotyl storage cells contained predominantly one large central vacuole (Fig. 2A and B). However, after tissue incubation in 200 mM sucrose for 24 h, a series of vacuole-derived organelles become evident (Fig. 2C and D), a condition scarcely observed when betaine was use ...
PDF
... blue exclusion test (usually The cell concentration was determined by and phase contrast microscopy (normally ...
... blue exclusion test (usually The cell concentration was determined by and phase contrast microscopy (normally ...
Selective Gene Delivery to Head and Neck Cancer Cells via an
... characteristics (7–10). In this regard, a number of distinct approaches have been tried in both animal models and human clinical trials. These approaches have included delivery of the p53 tumor suppressor gene or toxin-encoding genes, such as herpes simplex virus thymidine kinase and cytosine deamin ...
... characteristics (7–10). In this regard, a number of distinct approaches have been tried in both animal models and human clinical trials. These approaches have included delivery of the p53 tumor suppressor gene or toxin-encoding genes, such as herpes simplex virus thymidine kinase and cytosine deamin ...
Geographic Information Systems - CEProfs
... The statistic can be: majority, maximum, mean, median, minimum, minority, range, standard deviation, sum and variety. The neighborhood can have different shapes. To calculate neighborhood statistics, make the grid active, and click on Analysis/Neighborhood Statistics… ...
... The statistic can be: majority, maximum, mean, median, minimum, minority, range, standard deviation, sum and variety. The neighborhood can have different shapes. To calculate neighborhood statistics, make the grid active, and click on Analysis/Neighborhood Statistics… ...
Transfected Connexin45 Alters Gap Junction Permeability in Cells
... Alkali Solubilization and Immunoblot Alkali-insoluble material was isolated using a procedure modified from Hertzberg (15). Cells were scraped from two confluent 100-mm culture dishes into PBS, centrifuged, resuspended in 1 ml of 1 mM NaHCO3 containing protease and phosphatase inhibitors (1 mM PMSF, ...
... Alkali Solubilization and Immunoblot Alkali-insoluble material was isolated using a procedure modified from Hertzberg (15). Cells were scraped from two confluent 100-mm culture dishes into PBS, centrifuged, resuspended in 1 ml of 1 mM NaHCO3 containing protease and phosphatase inhibitors (1 mM PMSF, ...
CN Theveneau
... mesenchymal and highly migratory cells facing permissive areas containing extracellular matrix (ECM) and restrictive areas defined by negative cues present in the local environment. Consequently, NC cells would explore their direct surroundings and invade all areas free of inhibitors. Positive taxis ...
... mesenchymal and highly migratory cells facing permissive areas containing extracellular matrix (ECM) and restrictive areas defined by negative cues present in the local environment. Consequently, NC cells would explore their direct surroundings and invade all areas free of inhibitors. Positive taxis ...
The role of fibroblast growth factor in early Xenopus development
... the report by Smith (1987) of inducing activity secreted by a Xenopus cell line, we turned our attention to an investigation of known growth factors. In our initial screen, we tested a wide range of factors and found only three that were active. These were basic fibroblast growth factor (bFGF), embr ...
... the report by Smith (1987) of inducing activity secreted by a Xenopus cell line, we turned our attention to an investigation of known growth factors. In our initial screen, we tested a wide range of factors and found only three that were active. These were basic fibroblast growth factor (bFGF), embr ...
The Cell
... • Intermediate filaments range in diameter from 8–12 nanometers, larger than microfilaments but smaller than microtubules • They support cell shape and fix organelles in place • Intermediate filaments are more permanent cytoskeleton fixtures than the other two classes ...
... • Intermediate filaments range in diameter from 8–12 nanometers, larger than microfilaments but smaller than microtubules • They support cell shape and fix organelles in place • Intermediate filaments are more permanent cytoskeleton fixtures than the other two classes ...
The relative efficiency of homology
... UFBs (35). Thus, we sought to examine the effect of HDR deficiency on anaphase chromosome separation. As Rad51 is required in vertebrates for cellular viability (36,37), we characterized a set of defined mES cell lines with non-lethal HDR deficiencies. Specifically, we examined mES-RAD51KR cells that co ...
... UFBs (35). Thus, we sought to examine the effect of HDR deficiency on anaphase chromosome separation. As Rad51 is required in vertebrates for cellular viability (36,37), we characterized a set of defined mES cell lines with non-lethal HDR deficiencies. Specifically, we examined mES-RAD51KR cells that co ...
3.2 Cell Organelles Cells have an internal structure.
... DNA. – Centrioles form cilia and flagella. ...
... DNA. – Centrioles form cilia and flagella. ...
Chapter 7. The Cell: Basic Unit of Life
... Eukaryotic cells are more complex than prokaryotic cells within cytoplasm is a variety of ...
... Eukaryotic cells are more complex than prokaryotic cells within cytoplasm is a variety of ...
- Wiley Online Library
... mesenchymal and highly migratory cells facing permissive areas containing extracellular matrix (ECM) and restrictive areas defined by negative cues present in the local environment. Consequently, NC cells would explore their direct surroundings and invade all areas free of inhibitors. Positive taxis ...
... mesenchymal and highly migratory cells facing permissive areas containing extracellular matrix (ECM) and restrictive areas defined by negative cues present in the local environment. Consequently, NC cells would explore their direct surroundings and invade all areas free of inhibitors. Positive taxis ...
Laser-Micropipet Combination for Single-Cell Analysis
... resolving power, capillary electrophoresis is used for biochemical measurements on single cells; however, accurate measurements of many physiological parameters require sampling strategies that are considerably faster than those presently available. We have developed a laser-based technique to lyse ...
... resolving power, capillary electrophoresis is used for biochemical measurements on single cells; however, accurate measurements of many physiological parameters require sampling strategies that are considerably faster than those presently available. We have developed a laser-based technique to lyse ...
Insulin-like Growth Factor I Receptor Signaling in
... epidermal growth factor or serum and differentiate to a neuronal phenotype in N2 medium supplemented with bFGF at the nonpermissive temperature of 39°C (12, 13). We infected H19-7 cells with a retroviral vector expressing the human IGF-IR, and after selection, the mixed population was tested by West ...
... epidermal growth factor or serum and differentiate to a neuronal phenotype in N2 medium supplemented with bFGF at the nonpermissive temperature of 39°C (12, 13). We infected H19-7 cells with a retroviral vector expressing the human IGF-IR, and after selection, the mixed population was tested by West ...
Studies of vacuolar trafficking pathways regulated by RAB5 and
... indicating that these machinery components are recruited to a more complex trafficking network in plants. These results indicate that plants have developed a complex vacuolar transport system distinct from that of non-plant systems by assigning evolutionarily conserved machineries to unique traffick ...
... indicating that these machinery components are recruited to a more complex trafficking network in plants. These results indicate that plants have developed a complex vacuolar transport system distinct from that of non-plant systems by assigning evolutionarily conserved machineries to unique traffick ...
Correlating single cell motility with population growth dynamics
... percentage of tumblers and tumbling-runners are independent of the progression of the batch culture growth (Fig. 5). Our observations were confirmed by very low CCs between absorbance and percentage of tumblers and tumblingrunners (i.e. for data shown in Figure 5B and D respectively) in the growth c ...
... percentage of tumblers and tumbling-runners are independent of the progression of the batch culture growth (Fig. 5). Our observations were confirmed by very low CCs between absorbance and percentage of tumblers and tumblingrunners (i.e. for data shown in Figure 5B and D respectively) in the growth c ...
Author Guidelines for 8
... fatigue and bias. The essential precursor to velocity computation is the implementation of a robust multi-target tracking (MTT) algorithm. In this paper we propose a novel MTT algorithm based on a sequential Bayesian framework with the motivation of solving MTT in the context of cell velocity analys ...
... fatigue and bias. The essential precursor to velocity computation is the implementation of a robust multi-target tracking (MTT) algorithm. In this paper we propose a novel MTT algorithm based on a sequential Bayesian framework with the motivation of solving MTT in the context of cell velocity analys ...
Growth of curved and helical bacterial cells
... shape, the helical shape of C. cresentus is mainly created and maintained by differential cell wall growth induced by crescentin.15 Previous models have considered the elastic interaction of the crescentin bundle with the cell wall.21,23,24 In these models, the cell wall is treated as a static elast ...
... shape, the helical shape of C. cresentus is mainly created and maintained by differential cell wall growth induced by crescentin.15 Previous models have considered the elastic interaction of the crescentin bundle with the cell wall.21,23,24 In these models, the cell wall is treated as a static elast ...
Organization of Yeast Communities Cell Signals, Cell
... Biological Sciences, University of Missouri—Kansas City, 5007 Rockhill Rd., Kansas City, MO 66207. Phone: (816) 235-2578. Fax: (816) ...
... Biological Sciences, University of Missouri—Kansas City, 5007 Rockhill Rd., Kansas City, MO 66207. Phone: (816) 235-2578. Fax: (816) ...
Cannibalism and fratricide: mechanisms and raisons d`être
... the global transcription pattern at the onset of sporulation11. A complex network consisting of the histidine kinases KinA–E, the phosphorelay proteins Spo0F and Spo0B, several phosphatases and other transcriptional regulators controls the level of Spo0A~P in the cell. This network monitors and resp ...
... the global transcription pattern at the onset of sporulation11. A complex network consisting of the histidine kinases KinA–E, the phosphorelay proteins Spo0F and Spo0B, several phosphatases and other transcriptional regulators controls the level of Spo0A~P in the cell. This network monitors and resp ...
The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate
... Ziegelhoffer et al., 2000). Both farnesylated and geranylgeranylated proteins are involved in abscisic acid (ABA) signaling. The existence of farnesylated negative regulators of ABA signaling is inferred from the ABA hypersensitive phenotype of ENHANCED RESPONSE TO ABA1 (ERA1) mutants of Arabidopsis ...
... Ziegelhoffer et al., 2000). Both farnesylated and geranylgeranylated proteins are involved in abscisic acid (ABA) signaling. The existence of farnesylated negative regulators of ABA signaling is inferred from the ABA hypersensitive phenotype of ENHANCED RESPONSE TO ABA1 (ERA1) mutants of Arabidopsis ...
An in vitro System to Study Interactions between Bacteria and
... Wilson, 1975; Moon et al., 1977). However, these techniques do not provide much information on the various bacterial and host components involved at the molecular level. Such information can best be obtained by studying the interactions of radioactively labelled bacteria or bacterial components with ...
... Wilson, 1975; Moon et al., 1977). However, these techniques do not provide much information on the various bacterial and host components involved at the molecular level. Such information can best be obtained by studying the interactions of radioactively labelled bacteria or bacterial components with ...
THE SEPARATION OF DIFFERENT CELL CLASSES FROM
... ensure that the cells were fully active immediately on contact with the glass beads) with intermittent stirring with a Vortex mixer (Scientific Industries, Inc., Queens Village, N .Y.) (to minimize aggregation and adherence to the tube) . The suspension was then run onto the bead column, followed by ...
... ensure that the cells were fully active immediately on contact with the glass beads) with intermittent stirring with a Vortex mixer (Scientific Industries, Inc., Queens Village, N .Y.) (to minimize aggregation and adherence to the tube) . The suspension was then run onto the bead column, followed by ...
Can We Use Nanotechnology to Treat Cancer?
... Cancer is a complicated disease that causes cells to grow in a way that is different from normal, healthy cells. A normal cell in your body will divide until it becomes “old” or is damaged, when it dies in a special way called programed cell death. This dividing followed by programed cell death is a ...
... Cancer is a complicated disease that causes cells to grow in a way that is different from normal, healthy cells. A normal cell in your body will divide until it becomes “old” or is damaged, when it dies in a special way called programed cell death. This dividing followed by programed cell death is a ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.