Downloaded - The Journal of Cell Biology
... that p120 was a target by which signal transduction pathways regulate AJs. Given this, the seemingly contradictory effects of the JM region on adhesion might be explained if this region receives regulatory inputs that vary depending on cell type, possibly via posttranslational modification of p120. ...
... that p120 was a target by which signal transduction pathways regulate AJs. Given this, the seemingly contradictory effects of the JM region on adhesion might be explained if this region receives regulatory inputs that vary depending on cell type, possibly via posttranslational modification of p120. ...
Plasma Membrane Depolarization and Disturbed Na Homeostasis
... driven by the Na1 electrochemical gradient. The permeability of the plasma membrane to Na1 is very limited, allowing a slow entry of Na1 down the Na1 electrochemical gradient, which is normally balanced by Na1 extrusion via the Na1,K1/ATPase. If this balance is disturbed, the concentration of Na1 in ...
... driven by the Na1 electrochemical gradient. The permeability of the plasma membrane to Na1 is very limited, allowing a slow entry of Na1 down the Na1 electrochemical gradient, which is normally balanced by Na1 extrusion via the Na1,K1/ATPase. If this balance is disturbed, the concentration of Na1 in ...
BoNT_A Presentation UMass Dartmouth 081029
... intoxication with BoNTs, new small-molecule inhibitors of these toxins are critically needed. ...
... intoxication with BoNTs, new small-molecule inhibitors of these toxins are critically needed. ...
Development of Competitive Direct Enzyme
... (HPLC; Stead and Richards, 1996, 1997; Graham et al., 1997). Moreover, enzyme-linked immunosorbent assay (ELISA) using polyclonal (Tsay et al., 1980; Place et al., 1983) or monoclonal antibody (Berkowitz and Webert, 1986; Ploczekova and Foldes, 1992; Hanes and Herring, 2001) has been widely used for ...
... (HPLC; Stead and Richards, 1996, 1997; Graham et al., 1997). Moreover, enzyme-linked immunosorbent assay (ELISA) using polyclonal (Tsay et al., 1980; Place et al., 1983) or monoclonal antibody (Berkowitz and Webert, 1986; Ploczekova and Foldes, 1992; Hanes and Herring, 2001) has been widely used for ...
Donohoe, B.S., B. - University of Colorado-MCDB
... Golgi stacks are clustered around the centrosomes (32). To enable efficient cargo trafficking between these two membrane systems, mammalian cells have evolved an intermediate membrane compartment, the ER-Golgi intermediate compartment (ERGIC), within which Golgi cisterna elements are pre-assembled p ...
... Golgi stacks are clustered around the centrosomes (32). To enable efficient cargo trafficking between these two membrane systems, mammalian cells have evolved an intermediate membrane compartment, the ER-Golgi intermediate compartment (ERGIC), within which Golgi cisterna elements are pre-assembled p ...
Mycobacteria Target DC-SIGN to Suppress Dendritic Cell Function
... suppress cellular immune responses. Although DCs are not the primary targets for infection by mycobacteria, the specific function of DCs in the cellular immune response seems to be modulated by mycobacteria (7). Thus, knowledge about the interaction of DCs with mycobacteria and mycobacterial compone ...
... suppress cellular immune responses. Although DCs are not the primary targets for infection by mycobacteria, the specific function of DCs in the cellular immune response seems to be modulated by mycobacteria (7). Thus, knowledge about the interaction of DCs with mycobacteria and mycobacterial compone ...
Interleukin 12 p40 Production by Barrier Epithelial Cells during
... chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid was subjected to 10% PAGE under nonreducing or reducing (10 mM d ...
... chromogen. In these experiments, the BAL fluid (without concentration) was subjected to additional analyses of TNF-␣ levels by ELISA (with a sensitivity of 5 pg/ml) and IL-12 by Western blotting. For immunoblotting, cell-free BAL fluid was subjected to 10% PAGE under nonreducing or reducing (10 mM d ...
Cdc6 in S phase in human cells - Journal of Cell Science
... replication can now be achieved entirely in vitro using soluble protein extracts made from human cells synchronised in S phase. A single round of DNA synthesis can be induced in nuclei from HeLa cells chemically synchronised in late G1 (Krude et al., 1997) or nuclei from mouse cells released from qu ...
... replication can now be achieved entirely in vitro using soluble protein extracts made from human cells synchronised in S phase. A single round of DNA synthesis can be induced in nuclei from HeLa cells chemically synchronised in late G1 (Krude et al., 1997) or nuclei from mouse cells released from qu ...
Spatial and temporal in vivo analysis of circulating
... immune responses are cells called hemocytes, which kill pathogens via phagocytosis and via the production of soluble antimicrobial factors. Mosquito hemocytes are circulated throughout the hemocoel (body cavity) by the swift flow of hemolymph (blood), and data show that some hemocytes also exist as ...
... immune responses are cells called hemocytes, which kill pathogens via phagocytosis and via the production of soluble antimicrobial factors. Mosquito hemocytes are circulated throughout the hemocoel (body cavity) by the swift flow of hemolymph (blood), and data show that some hemocytes also exist as ...
Isolation and characterization of the Pin1/Ess1p homologue in
... activation (Liu et al., 2001). Recently, Pin1 has been shown to be overexpressed in breast cancer, and to increase expression of cyclin D1 as a result of its ability to bind to c-Jun phosphorylated at the Ser63/73.Pro motifs in its transactivation domain, and thereby stimulate cyclin D1 promoter act ...
... activation (Liu et al., 2001). Recently, Pin1 has been shown to be overexpressed in breast cancer, and to increase expression of cyclin D1 as a result of its ability to bind to c-Jun phosphorylated at the Ser63/73.Pro motifs in its transactivation domain, and thereby stimulate cyclin D1 promoter act ...
Dentin-pulp complex
... Fibrous Matrix: Mostly reticular fibres and collagen fibres (Type I and Type III). Ground substance: Act as a medium to transport nutrients to cells and metabolites of the cell to the blood vessels. ...
... Fibrous Matrix: Mostly reticular fibres and collagen fibres (Type I and Type III). Ground substance: Act as a medium to transport nutrients to cells and metabolites of the cell to the blood vessels. ...
Binding of Thrombin to Subendothelial Extracellular Matrix
... with central roles in hemostasis, to the subendothelial extracellular matrix (ECM) produced by cultured endothelial cells. This substrate provides a thrombogenic surface where hemostasis is initiated. Binding was saturable and equilibrium was achieved after 3 h incubation with '25I-a-thrombin. Scatc ...
... with central roles in hemostasis, to the subendothelial extracellular matrix (ECM) produced by cultured endothelial cells. This substrate provides a thrombogenic surface where hemostasis is initiated. Binding was saturable and equilibrium was achieved after 3 h incubation with '25I-a-thrombin. Scatc ...
Alternative splicing of human cyclin E - Journal of Cell Science
... sequence of the insert was determined and compared to the corresponding region of cyclin E. The sequence of the relevant region around the splice junction is shown in Fig. 2a and b. It is obvious that the sequence of cyclin Es is identical to that of cyclin E until the position of the SA site is rea ...
... sequence of the insert was determined and compared to the corresponding region of cyclin E. The sequence of the relevant region around the splice junction is shown in Fig. 2a and b. It is obvious that the sequence of cyclin Es is identical to that of cyclin E until the position of the SA site is rea ...
Auxin transport – shaping the plant
... been disrupted have indeed suggested that auxin transport mediates the lateral distribution of auxin [10,29]. The search for molecular support for this concept brought about the identi®cation of PIN3 [10]. PIN3 is involved in hypocotyl and root tropisms, and is localized in the lateral endodermi ...
... been disrupted have indeed suggested that auxin transport mediates the lateral distribution of auxin [10,29]. The search for molecular support for this concept brought about the identi®cation of PIN3 [10]. PIN3 is involved in hypocotyl and root tropisms, and is localized in the lateral endodermi ...
Focusing on unpolymerized actin.
... subunits with bound ATP and will not increase the fraction of ADP-actin present. Interestingly, its relative affinity for ATP- and ADP-actin roughly parallels the relative affinities of actin filaments for these monomeric forms. This may allow thymosin/34 to serve as a physiological buffer for both ...
... subunits with bound ATP and will not increase the fraction of ADP-actin present. Interestingly, its relative affinity for ATP- and ADP-actin roughly parallels the relative affinities of actin filaments for these monomeric forms. This may allow thymosin/34 to serve as a physiological buffer for both ...
Adeno-Associated Virus Vectors for Gene Therapy - diss.fu
... 1.1.1 Genome Structure and Expression of the Viral Proteins The genomes of the different AAV serotypes consist of linear, single-stranded DNA molecules with a length of approximately 4700 nucleotides. Generally, the genome structures are mostly identical for all AAV serotypes. However, most research ...
... 1.1.1 Genome Structure and Expression of the Viral Proteins The genomes of the different AAV serotypes consist of linear, single-stranded DNA molecules with a length of approximately 4700 nucleotides. Generally, the genome structures are mostly identical for all AAV serotypes. However, most research ...
understanding the role of sumoylation in regulating lkb1 function
... Ritho, Joan W., "UNDERSTANDING THE ROLE OF SUMOYLATION IN REGULATING LKB1 FUNCTION" (2015). UT GSBS Dissertations and Theses (Open Access). 570. ...
... Ritho, Joan W., "UNDERSTANDING THE ROLE OF SUMOYLATION IN REGULATING LKB1 FUNCTION" (2015). UT GSBS Dissertations and Theses (Open Access). 570. ...
NCAM Polypeptides in Heart Development: Association with Z Discs
... outgrowth, while the MSD region had no effect in these experiments (Doherty et al., 1992). Recent experiments suggest that the MSD region plays a role in myoblast fusion (Peck and Walsh, 1993). Possible functions for the MSD region may be related to the fact that it contains an O-linked carbohydrate ...
... outgrowth, while the MSD region had no effect in these experiments (Doherty et al., 1992). Recent experiments suggest that the MSD region plays a role in myoblast fusion (Peck and Walsh, 1993). Possible functions for the MSD region may be related to the fact that it contains an O-linked carbohydrate ...
Ca signaling and early embryonic patterning during zebrafish
... (Dii) head development and (Diii) tail formation in the early SP. (A) CP: Animal pole view of a representative embryo showing the first two cell division cycles (i.e., from the 1- to 4-cell stage). The luminescent images (pseudocolor panels, labeled a-m) represent 1 min of accumulated light with a 2 ...
... (Dii) head development and (Diii) tail formation in the early SP. (A) CP: Animal pole view of a representative embryo showing the first two cell division cycles (i.e., from the 1- to 4-cell stage). The luminescent images (pseudocolor panels, labeled a-m) represent 1 min of accumulated light with a 2 ...
CONTRIBUTION OF DYSFERLIN-CONTAINING
... Dysferlinopathy and a novel pathway to muscular dystrophy: Dysferlin deficiency results in muscle disease: Dysferlin is expressed in most tissues at low levels, with highest expression in adult skeletal and cardiac muscle [16, 17]. Western blot analysis using an antibody to the dysferlin protein ide ...
... Dysferlinopathy and a novel pathway to muscular dystrophy: Dysferlin deficiency results in muscle disease: Dysferlin is expressed in most tissues at low levels, with highest expression in adult skeletal and cardiac muscle [16, 17]. Western blot analysis using an antibody to the dysferlin protein ide ...
375 Na+/Ca2+ ANTIPORT IN THE MAMMALIAN HEART
... with the cytoskeleton may be imporant for both functional activity and the distribution of the antiporter on the membrane surface. The importance of membrane surface distribution for antiporter function is not yet well understood. In smooth muscle cells, the Na+/Ca2+ antiporter and the Na+/K+-ATPase ...
... with the cytoskeleton may be imporant for both functional activity and the distribution of the antiporter on the membrane surface. The importance of membrane surface distribution for antiporter function is not yet well understood. In smooth muscle cells, the Na+/Ca2+ antiporter and the Na+/K+-ATPase ...
Three distinct ribosome assemblies modulated by translation are the
... For each sample under consideration, manual object selection was used to generate galleries of images. We aligned rotationally and translationally 544 objects for the LMW sample, 567 objects for the MMW sample, and 700 objects for the HMW sample. Bars, 100 nm. (b) To identify the most populated clas ...
... For each sample under consideration, manual object selection was used to generate galleries of images. We aligned rotationally and translationally 544 objects for the LMW sample, 567 objects for the MMW sample, and 700 objects for the HMW sample. Bars, 100 nm. (b) To identify the most populated clas ...
A heritable switch in carbon source utilization driven
... repression,’’ with capital letters indicating dominance and brackets indicating its non-Mendelian character). [GAR+] appears spontaneously at a high rate and is transmissible by non-Mendelian, cytoplasmic inheritance. Several lines of evidence suggest that the prion state involves a complex between ...
... repression,’’ with capital letters indicating dominance and brackets indicating its non-Mendelian character). [GAR+] appears spontaneously at a high rate and is transmissible by non-Mendelian, cytoplasmic inheritance. Several lines of evidence suggest that the prion state involves a complex between ...
Spectrin functions upstream of ankyrin in a spectrin cytoskeleton
... 2004c). From these results, the authors concluded that ankyrin was upstream of spectrin in the spectrin cytoskeleton assembly pathway and that its function was independent of spectrin. However, a study of βIV spectrin knockout mice revealed that βIV spectrin and ankyrin-G are dependent on one anothe ...
... 2004c). From these results, the authors concluded that ankyrin was upstream of spectrin in the spectrin cytoskeleton assembly pathway and that its function was independent of spectrin. However, a study of βIV spectrin knockout mice revealed that βIV spectrin and ankyrin-G are dependent on one anothe ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.