K-Ras is essential for normal fetal liver erythropoiesis
... c-kit⫹ fetal liver cells were plated in 96-well plates with no serum in the presence of varying concentrations of either EPO or KitL alone or in combination. After 48 hours in culture, cells were stained with annexin V–FITC followed by FACS analysis. For thymidine incorporation assays, 10 000 c-kit⫹ ...
... c-kit⫹ fetal liver cells were plated in 96-well plates with no serum in the presence of varying concentrations of either EPO or KitL alone or in combination. After 48 hours in culture, cells were stained with annexin V–FITC followed by FACS analysis. For thymidine incorporation assays, 10 000 c-kit⫹ ...
Regulation of the endothelial cell cycle by the ubiquitin
... However, quantitative proteomics in yeast show that these linkages are abundant in vivo and that they all target proteins for degradation with only partially redundant functions.12 Besides ubiquitination, proteins could also be modified by the small Ub-like modifier SUMO.13 SUMOylation is reversible ...
... However, quantitative proteomics in yeast show that these linkages are abundant in vivo and that they all target proteins for degradation with only partially redundant functions.12 Besides ubiquitination, proteins could also be modified by the small Ub-like modifier SUMO.13 SUMOylation is reversible ...
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... The elimination of senescent cells is an attractive avenue for developing new interventions to treat age-related pathologies, and several laboratories are searching for small molecules that might be of potential interest for future clinical studies (see (Dorr et al., 2013) for an example). In the sk ...
... The elimination of senescent cells is an attractive avenue for developing new interventions to treat age-related pathologies, and several laboratories are searching for small molecules that might be of potential interest for future clinical studies (see (Dorr et al., 2013) for an example). In the sk ...
Cell Structure and Function Chapter 4 Outline Cell Theory Cell Size
... Cell theory states all organisms are composed of cells and that cells come only from preexisting cells. – One of unifying concepts of biology. ...
... Cell theory states all organisms are composed of cells and that cells come only from preexisting cells. – One of unifying concepts of biology. ...
An Improved cDNA Library Generation Protocol for Transcriptome
... with RefSeq masking, producing uniquely mapped reads and % reads that mapped to RefSeq annotations. The number of genes identified in each library was determined by the number of genes with a RPKM ≥0.1. Expression levels of different genes were obtained using CLC Genomics Workbench after mapping to ...
... with RefSeq masking, producing uniquely mapped reads and % reads that mapped to RefSeq annotations. The number of genes identified in each library was determined by the number of genes with a RPKM ≥0.1. Expression levels of different genes were obtained using CLC Genomics Workbench after mapping to ...
Dimensional analysis of Expansive Growth of Cells with Walls
... involved in processes of interest, to organize large quantities of experimental data, and to provide scaling laws for models. The overall process is to obtain dimensionless groups of variables (Π parameters) involved in the process of interest and explore the relationship between the Π parameters, e ...
... involved in processes of interest, to organize large quantities of experimental data, and to provide scaling laws for models. The overall process is to obtain dimensionless groups of variables (Π parameters) involved in the process of interest and explore the relationship between the Π parameters, e ...
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... of mouse embryonic stem cells (mESCs) generates a population with many of the properties of the NMp. A single-cell analysis of gene expression within this population reveals signatures that are characteristic of stem cell populations. Furthermore, when this activation is triggered in three-dimension ...
... of mouse embryonic stem cells (mESCs) generates a population with many of the properties of the NMp. A single-cell analysis of gene expression within this population reveals signatures that are characteristic of stem cell populations. Furthermore, when this activation is triggered in three-dimension ...
Bronxville HS Page 1 of 8 AP Biology Mr. Ippolito AP Biology
... one short year prior to the AP exam in May. The course requires a significant amount of reading, new terminology, memorization, analysis and understanding of multiple topics. To succeed you must be self-motivated and capable of developing and sticking to a rigorous schedule. It is extremely importan ...
... one short year prior to the AP exam in May. The course requires a significant amount of reading, new terminology, memorization, analysis and understanding of multiple topics. To succeed you must be self-motivated and capable of developing and sticking to a rigorous schedule. It is extremely importan ...
Primordial germ cells in the mouse embryo during
... of ALP-positive cells in a pocket-like mesodermal structure at the base of the allantois. Most of the cells now contained a very well defined ALP spot, although some positive cells still appeared spotless. Of the 80-85 cells per embryo that contained a spot (Table 1), most were located in the extrae ...
... of ALP-positive cells in a pocket-like mesodermal structure at the base of the allantois. Most of the cells now contained a very well defined ALP spot, although some positive cells still appeared spotless. Of the 80-85 cells per embryo that contained a spot (Table 1), most were located in the extrae ...
Chapter 6 A Tour of the Cell Multiple-Choice Questions
... 25) Which of the following statements correctly describes some aspect of protein disposal from prokaryotic cells? A) Prokaryotes are unlikely to be able to excrete proteins because they lack an endomembrane system. B) The mechanism of protein excretion in prokaryotes is probably the same as that in ...
... 25) Which of the following statements correctly describes some aspect of protein disposal from prokaryotic cells? A) Prokaryotes are unlikely to be able to excrete proteins because they lack an endomembrane system. B) The mechanism of protein excretion in prokaryotes is probably the same as that in ...
Epithelial to mesenchymal transition during gastrulation
... basement membrane matrix covering the vegetal plate cells shows discontinuity underneath ingressing cells, and is resealed as a continuous layer after ingression of all primary mesenchymal cells. The delamination of secondary mesenchymal cells from the tip of invaginating archenteron is less charact ...
... basement membrane matrix covering the vegetal plate cells shows discontinuity underneath ingressing cells, and is resealed as a continuous layer after ingression of all primary mesenchymal cells. The delamination of secondary mesenchymal cells from the tip of invaginating archenteron is less charact ...
Sordaria Lab
... 1. Disinfect the work surface and wash hands. 2. When ready for use, label two of the cornmeal-glucose-yeast agar dishes “wild,” two “gray”, and two “tan.” 3. Using aseptic technique, inoculate the dishes with the appropriate culture. Remove the top from the tube of the wild-type Sordaria fimicola, ...
... 1. Disinfect the work surface and wash hands. 2. When ready for use, label two of the cornmeal-glucose-yeast agar dishes “wild,” two “gray”, and two “tan.” 3. Using aseptic technique, inoculate the dishes with the appropriate culture. Remove the top from the tube of the wild-type Sordaria fimicola, ...
11-13-12 Meiosis FILL IN THE BLANK NOTES
... _____________________ – Two exact copies of DNA (each single copy called a chromatid) makes up a ______________________. ...
... _____________________ – Two exact copies of DNA (each single copy called a chromatid) makes up a ______________________. ...
Role of Silicon in Diatom Metabolism. Messenger
... 1980), mRNAs were analysed. This not only permitted the extent of the control of gene expression by silicon to be better defined, but also allowed the timing of individual changes to be more precisely determined. Thus, RNA extracted from polysomes isolated during synchrony was translated in vitro an ...
... 1980), mRNAs were analysed. This not only permitted the extent of the control of gene expression by silicon to be better defined, but also allowed the timing of individual changes to be more precisely determined. Thus, RNA extracted from polysomes isolated during synchrony was translated in vitro an ...
Hyaluronidase enhances the activity of Adriamycin in breast cancer
... Prehm (1990) discussed the observation that high-molecular-mass hyaluronate inhibits cell growth, whereas small fragments are stimulatory. In addition, it is known that hyaluronidase treatment of living cells stimulates the synthesis of hyaluronic acid (Philipson et al. 1985; Larnier et al. 1989), w ...
... Prehm (1990) discussed the observation that high-molecular-mass hyaluronate inhibits cell growth, whereas small fragments are stimulatory. In addition, it is known that hyaluronidase treatment of living cells stimulates the synthesis of hyaluronic acid (Philipson et al. 1985; Larnier et al. 1989), w ...
Isolation of Spherosomes with Lysosome Characteristics from
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
Cancer stem cell definitions and terminology: the devil is - e
... implications, but its investigation has been hampered both by a lack of consistency in the terms used for these cells and by how they are defined. Evidence of their heterogeneous origins, frequencies and their genomic, as well as their phenotypic and functional, properties has added to the confusion ...
... implications, but its investigation has been hampered both by a lack of consistency in the terms used for these cells and by how they are defined. Evidence of their heterogeneous origins, frequencies and their genomic, as well as their phenotypic and functional, properties has added to the confusion ...
Spatial and temporal distribution of the adherens
... occurring during the development of feathers correlates with periodic and reiterative expressions of both N-CAM and L-CAM (Chuong & Edelman. 1985o.6; Crossing al. 1985). The study of distribution of the various known CAMs during embryogenesis has led to the distinction of two families: on one hand, ...
... occurring during the development of feathers correlates with periodic and reiterative expressions of both N-CAM and L-CAM (Chuong & Edelman. 1985o.6; Crossing al. 1985). The study of distribution of the various known CAMs during embryogenesis has led to the distinction of two families: on one hand, ...
Insulin-like Growth Factor I Receptor Signaling in
... expression of neurofilaments can be used as a marker of neuronal differentiation (27). The immunofluorescent staining of NF68 was performed as described by Kuo et al. (27), with some modifications. Cells were plated at a density of 105 cells/35-mm, poly-L-lysine (Sigma)-coated plate in medium contai ...
... expression of neurofilaments can be used as a marker of neuronal differentiation (27). The immunofluorescent staining of NF68 was performed as described by Kuo et al. (27), with some modifications. Cells were plated at a density of 105 cells/35-mm, poly-L-lysine (Sigma)-coated plate in medium contai ...
Humoral and contact interactions in astroglia/stem cell co
... communicated with glial cells only through the culture medium. Co-cultures were maintained either under serum-free conditions or in the presence of 5% FCS. There were no differences in the recorded parameters between the cultures maintained in any of the three chemically defined media: Neurobasal-B27 ...
... communicated with glial cells only through the culture medium. Co-cultures were maintained either under serum-free conditions or in the presence of 5% FCS. There were no differences in the recorded parameters between the cultures maintained in any of the three chemically defined media: Neurobasal-B27 ...
Regents Biology - I Heart Science
... stimulates immune system to produce antibodies to invader rapid response if future exposure ...
... stimulates immune system to produce antibodies to invader rapid response if future exposure ...
Cell Structure/Function
... 11. In which cell of the human body would you expect to find the most mitochondria? Your Answer: Correct Answer: 12. Which organelle is responsible for photosynthesis? Your Answer: Correct Answer: 13. In the diagram below, Cell I and II represent typical cells. In both cells, organelle 5 is ...
... 11. In which cell of the human body would you expect to find the most mitochondria? Your Answer: Correct Answer: 12. Which organelle is responsible for photosynthesis? Your Answer: Correct Answer: 13. In the diagram below, Cell I and II represent typical cells. In both cells, organelle 5 is ...
MORPHOLOGICAL ALTERATIONS
... • Usually involves large numbers of cells. • Necrotic cells may spill their contents, causing inflammation and injury to neighboring cells. Many types; • Coagulative necrosis, • Caseous necrosis • Liquefactive necrosis • Fat necrosis • Fibrinoid necrosis ...
... • Usually involves large numbers of cells. • Necrotic cells may spill their contents, causing inflammation and injury to neighboring cells. Many types; • Coagulative necrosis, • Caseous necrosis • Liquefactive necrosis • Fat necrosis • Fibrinoid necrosis ...
Full Text - Digital Access to Scholarship at Harvard
... (SK29-MEL-1) and melanocytes. Cultured melanoma cells and melanocytes were stained and analyzed by flow cytometry. Bar diagram shows pooled data of 5 independent experiments (means ± SD). B. GARP-mRNA expression in UKRV-Mel-15A, SK29-MEL-1 and melanocytes was analyzed and normalized to the housekeep ...
... (SK29-MEL-1) and melanocytes. Cultured melanoma cells and melanocytes were stained and analyzed by flow cytometry. Bar diagram shows pooled data of 5 independent experiments (means ± SD). B. GARP-mRNA expression in UKRV-Mel-15A, SK29-MEL-1 and melanocytes was analyzed and normalized to the housekeep ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.