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Isolation of Spherosomes with Lysosome
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
Isolation of Spherosomes with Lysosome Characteristics from
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
... activity after separation of the particulate compo nents from the soluble material indicates the pos sibility of an interaction between hydrolases and soluble factors. In fact the high speed supernatant contains an inhibitory factor for acid protease and phosphatase as demonstrated by determ inati ...
Hunt, D.E. Motility in the marine environment: an adaptive response
... 1051. No attempt has been made to purify the non-axenic strains from their co-existing heterotrophic organisms although they have been in culture for many years. Cyanobacteria were grown in a Sargasso-seawater based medium containing no combined nitrogen so that cells would grow diazotrophically wit ...
... 1051. No attempt has been made to purify the non-axenic strains from their co-existing heterotrophic organisms although they have been in culture for many years. Cyanobacteria were grown in a Sargasso-seawater based medium containing no combined nitrogen so that cells would grow diazotrophically wit ...
REACTION OF THE GOBLET CELLS TO THE CHOLINERGIC
... secretion, they mostly did not take part in the further secretory cycles but they degenerated and were gradually expelled from the epithelium. In our previous studies we demonstrated that the degeneration of more than half of the goblet cells in the epithelium was always followed by a massive differ ...
... secretion, they mostly did not take part in the further secretory cycles but they degenerated and were gradually expelled from the epithelium. In our previous studies we demonstrated that the degeneration of more than half of the goblet cells in the epithelium was always followed by a massive differ ...
Viscoelastic dissipation stabilizes cell shape changes
... reference configuration, allowing forces exerted transiently to generate persistent shape changes. In order for the latter to be true, dissipation should occur on time scales typically shorter, or commensurate with, but definitely not much longer than the timescale of force generation. Hence it is o ...
... reference configuration, allowing forces exerted transiently to generate persistent shape changes. In order for the latter to be true, dissipation should occur on time scales typically shorter, or commensurate with, but definitely not much longer than the timescale of force generation. Hence it is o ...
Cellular Force Microscopy for in Vivo
... model of a monolayered, homogeneous tissue. Two types of experiments were conducted on epidermal cells with CFM: stiffness mapping with noninvasive, small indentation scans covering several cells, and cell wall puncture using very high forces. The mechanical effects of turgor pressure were investiga ...
... model of a monolayered, homogeneous tissue. Two types of experiments were conducted on epidermal cells with CFM: stiffness mapping with noninvasive, small indentation scans covering several cells, and cell wall puncture using very high forces. The mechanical effects of turgor pressure were investiga ...
Surface probe measurements of the elasticity of sectioned tissue
... Surface probe measurements of the elasticity of thin-film matrices as well as biological samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by Atomic Force Microscopy (AFM) within the smooth muscle cell (SMC)-ric ...
... Surface probe measurements of the elasticity of thin-film matrices as well as biological samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by Atomic Force Microscopy (AFM) within the smooth muscle cell (SMC)-ric ...
Antifolate Polyglutamylation and Competitive Drug Displacement at
... pared from radiolabeled folie acid and unlabeled FH2, respectively, as described by Moran and Colman (18), except that all procedures were conducted under a nitrogen atmosphere as described below. Other chemicals were obtained from commercial sources. Cell Culture. The murine L1210 leukemia was grow ...
... pared from radiolabeled folie acid and unlabeled FH2, respectively, as described by Moran and Colman (18), except that all procedures were conducted under a nitrogen atmosphere as described below. Other chemicals were obtained from commercial sources. Cell Culture. The murine L1210 leukemia was grow ...
Biology IGCSE FALL 2011_2012 - Biology
... absorbing and assimilating them Fish • excretion as removal from organisms of toxic materials, the waste products of metabolism Amphibians (chemical reactions in cells including respiration) Reptiles and substances in excess of requirements Birds • respiration as the chemical reactions that break Ma ...
... absorbing and assimilating them Fish • excretion as removal from organisms of toxic materials, the waste products of metabolism Amphibians (chemical reactions in cells including respiration) Reptiles and substances in excess of requirements Birds • respiration as the chemical reactions that break Ma ...
Unusual Prokaryotic Envelope Cyanobacterial Cell Walls
... peptidoglycan of gram-positive bacteria, with a thickness ranging from 20 to 40 nm, usually forms a physical barrier for the dye. In gram-negative bacteria, however, with their relatively thin sacculus of 2 to 6 nm, the stain can easily be washed out. Together with other characteristics, such as the ...
... peptidoglycan of gram-positive bacteria, with a thickness ranging from 20 to 40 nm, usually forms a physical barrier for the dye. In gram-negative bacteria, however, with their relatively thin sacculus of 2 to 6 nm, the stain can easily be washed out. Together with other characteristics, such as the ...
Objectives
... 5. For simple epithelium, observe the cell shape and try to locate a basement membrane and other details, such as any goblet cells, microvilli or cilia that might be present. Microvilli and cilia can be seen best using the 100X (oil immersion) objective. For stratified epithelium, observe the shape ...
... 5. For simple epithelium, observe the cell shape and try to locate a basement membrane and other details, such as any goblet cells, microvilli or cilia that might be present. Microvilli and cilia can be seen best using the 100X (oil immersion) objective. For stratified epithelium, observe the shape ...
TKRP125, a kinesin-related protein involved in the centrosome
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
... that UV irradiation of the equatorial region of Haemanthus endosperm cells resulted in the disruption of phragmoplast microtubules that extended from the irradiated site. The phragmoplast in plasma membrane-permeabilized endosperm cells of Haemanthus incorporated exogenously applied tubulin at the e ...
Objectives
... 5. For simple epithelium, observe the cell shape and try to locate a basement membrane and other details, such as any goblet cells, microvilli or cilia that might be present. Microvilli and cilia can be seen best using the 100X (oil immersion) objective. For stratified epithelium, observe the shape ...
... 5. For simple epithelium, observe the cell shape and try to locate a basement membrane and other details, such as any goblet cells, microvilli or cilia that might be present. Microvilli and cilia can be seen best using the 100X (oil immersion) objective. For stratified epithelium, observe the shape ...
A New Model of the Human Atrial Myocyte with Variable T
... and regular contraction, underlain by rapid and irregular electrical activity in atrial tissue. AF is common and can have serious consequences, including a substantially increased risk of stroke, heart failure, and mortality [1]. Due to the growing epidemic of AF prevalence and the resulting strain ...
... and regular contraction, underlain by rapid and irregular electrical activity in atrial tissue. AF is common and can have serious consequences, including a substantially increased risk of stroke, heart failure, and mortality [1]. Due to the growing epidemic of AF prevalence and the resulting strain ...
Guidleine on potency testing of cell based immunotherapy medicinal
... targeting of interactions between the immune system and the tumour constitute a complex approach of which the precise mechanisms of action are often not fully understood. In the scientific literature, cell based immunotherapy products for the treatment of cancer are sometimes called cell based tumou ...
... targeting of interactions between the immune system and the tumour constitute a complex approach of which the precise mechanisms of action are often not fully understood. In the scientific literature, cell based immunotherapy products for the treatment of cancer are sometimes called cell based tumou ...
Developmental control of a G1-S transcriptional program in Drosophila
... Dramatic cell cycle regulation occurs during embryogenesis where precise and stereotyped programs of cell division guide orderly morphogenesis (Foe et al., 1993). In Drosophila, the mother supplies the egg with a plentiful reservoir of cell cycle components so that the earliest cycles are relatively ...
... Dramatic cell cycle regulation occurs during embryogenesis where precise and stereotyped programs of cell division guide orderly morphogenesis (Foe et al., 1993). In Drosophila, the mother supplies the egg with a plentiful reservoir of cell cycle components so that the earliest cycles are relatively ...
Reactivity of murine cytokine fusion toxin, diphtheria toxin390-murine
... Myeloid leukemias can express interleukin-3 receptors (IL3R). Therefore, as an antileukemia drug, a fusion immunotoxin was synthesized consisting of the murine IL-3 (mlL-3) gene spliced to a truncated form of the diphtheria toxin (DT390) genecoding for a molecule that retained full enzymatic activit ...
... Myeloid leukemias can express interleukin-3 receptors (IL3R). Therefore, as an antileukemia drug, a fusion immunotoxin was synthesized consisting of the murine IL-3 (mlL-3) gene spliced to a truncated form of the diphtheria toxin (DT390) genecoding for a molecule that retained full enzymatic activit ...
Accuratus AccuMed 540 v2 Backlit VESA
... The AccuMed 540 Backlit keyboard offers adjustable LED red backlit illumination and new ultra slim keys whilst still offering an excellent tactile and responsive feel. The keyboard can be fully cleaned and wiped over in literally seconds and also has a handy power on/off button, enabling you to turn ...
... The AccuMed 540 Backlit keyboard offers adjustable LED red backlit illumination and new ultra slim keys whilst still offering an excellent tactile and responsive feel. The keyboard can be fully cleaned and wiped over in literally seconds and also has a handy power on/off button, enabling you to turn ...
Emerging roles for lipids in non-apoptotic cell death
... recent years many non-apoptotic RCD pathways have been described, including necroptosis, pyroptosis, parthanatos, ferroptosis and several others.2–7 These pathways are known or thought to contribute to cell death following viral infection, bacterial infection, neurodegeneration, ischemia-reperfusion ...
... recent years many non-apoptotic RCD pathways have been described, including necroptosis, pyroptosis, parthanatos, ferroptosis and several others.2–7 These pathways are known or thought to contribute to cell death following viral infection, bacterial infection, neurodegeneration, ischemia-reperfusion ...
Targeted disruption of the mouse E-Ras gene
... described elsewhere (Y. Tokuzawa et al., Mol. Cell. Bio., in press). pCAG-gw-IH was constructed as follows: cDNA of hygromycin resistance gene was amplified from pHPCAG with primers hyg.upper and hyg.lower. Additional PCR with primers attB1 and attB2 was performed and PCR product was inserted into p ...
... described elsewhere (Y. Tokuzawa et al., Mol. Cell. Bio., in press). pCAG-gw-IH was constructed as follows: cDNA of hygromycin resistance gene was amplified from pHPCAG with primers hyg.upper and hyg.lower. Additional PCR with primers attB1 and attB2 was performed and PCR product was inserted into p ...
The role of the cytoskeleton in the polarized growth of
... C. albicans. Here we report that actin is involved in, but is not the sole factor required for, the control of apical growth in C. albicans, and that MTs are also involved in such control. METHODS Culture. Candida albicans strain FC18 (Magee e t a/., 1788) was used in this study. Cells were cultured ...
... C. albicans. Here we report that actin is involved in, but is not the sole factor required for, the control of apical growth in C. albicans, and that MTs are also involved in such control. METHODS Culture. Candida albicans strain FC18 (Magee e t a/., 1788) was used in this study. Cells were cultured ...
Kinetics of antiviral CD8 T cell responses during primary and post
... immunity [2,3,14]. This was most convincingly shown by T cell subset depletion experiments in cattle and mice [14,15]. Depletion of CD8 T cells in gnotobiotic calves resulted in more prolonged virus shedding and more severe pathologic lesions (i.e., more extensive lung consolidation), after bRSV inf ...
... immunity [2,3,14]. This was most convincingly shown by T cell subset depletion experiments in cattle and mice [14,15]. Depletion of CD8 T cells in gnotobiotic calves resulted in more prolonged virus shedding and more severe pathologic lesions (i.e., more extensive lung consolidation), after bRSV inf ...
Regulation of the endothelial cell cycle by the ubiquitin
... However, quantitative proteomics in yeast show that these linkages are abundant in vivo and that they all target proteins for degradation with only partially redundant functions.12 Besides ubiquitination, proteins could also be modified by the small Ub-like modifier SUMO.13 SUMOylation is reversible ...
... However, quantitative proteomics in yeast show that these linkages are abundant in vivo and that they all target proteins for degradation with only partially redundant functions.12 Besides ubiquitination, proteins could also be modified by the small Ub-like modifier SUMO.13 SUMOylation is reversible ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.