Etude in vitro de la stéatose hépatique induite par la - HAL
... E2, has proved a powerful tool for functional studies of HCV binding to and entry into Huh7 cells and other human liver cell lines [11, 12]. These pseudotyped retroviral particles differ from native serum-derived HCV virions, but studies with such particles identified both CD81 and SR-BI as potentia ...
... E2, has proved a powerful tool for functional studies of HCV binding to and entry into Huh7 cells and other human liver cell lines [11, 12]. These pseudotyped retroviral particles differ from native serum-derived HCV virions, but studies with such particles identified both CD81 and SR-BI as potentia ...
dextran by suspension-cultured plant cells
... applications of FITC as a fluorescent tracer have been limited to studies of cell-to-cell communication (Tucker, 1982; Goodwin, 1983; Terry and Robards, 1987) and the surface dynamics of protoplasts (Furtula et al. 1987; Walko et al. 1987). FITC-conjugated dextrans (FDs) have been frequently used as ...
... applications of FITC as a fluorescent tracer have been limited to studies of cell-to-cell communication (Tucker, 1982; Goodwin, 1983; Terry and Robards, 1987) and the surface dynamics of protoplasts (Furtula et al. 1987; Walko et al. 1987). FITC-conjugated dextrans (FDs) have been frequently used as ...
ABSTRACT SUSTAINED DELIVERY AND PHARMACODYNAMICS OF AN INTEGRIN
... Figure 4-5. Effect of EMD478761 on PCNA expression in HUVECs. .................... 74 Figure 4-6. Effect of EMD478761 on regulatory cell cycle protein p21 expression in HUVECs................................................................................................ 75 Figure 4-7. Effect of EM ...
... Figure 4-5. Effect of EMD478761 on PCNA expression in HUVECs. .................... 74 Figure 4-6. Effect of EMD478761 on regulatory cell cycle protein p21 expression in HUVECs................................................................................................ 75 Figure 4-7. Effect of EM ...
in Murine Mast Cells by Recombinant Stem Cell Factor
... to cellular membranes in response to nanomolar calcium concentrations (21-24). There also exists a family of low molecular weight PLA2 (- 14 kD in size, called group II PLA2) that is released from cells during activation (25-28). Group II PLA2 are present in high quantities in inflammatory sites suc ...
... to cellular membranes in response to nanomolar calcium concentrations (21-24). There also exists a family of low molecular weight PLA2 (- 14 kD in size, called group II PLA2) that is released from cells during activation (25-28). Group II PLA2 are present in high quantities in inflammatory sites suc ...
SYBR Green Cells-to-CT™ Control Kit
... fast cycling conditions, respectively) with user-supplied primer sets targeting genes of interest. The SYBR Green Cells-to-CT Control Kit is designed for use with the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits. It includes Xeno RNA Control, a synthetic RNA transcript with a unique sequenc ...
... fast cycling conditions, respectively) with user-supplied primer sets targeting genes of interest. The SYBR Green Cells-to-CT Control Kit is designed for use with the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits. It includes Xeno RNA Control, a synthetic RNA transcript with a unique sequenc ...
Rat embryonic ectoderm as renal isograft - Development
... Pedersen, 1978; Libbus & Hsu, 1980; Uno, 1982). The transformation of epithelial cells into mesenchymal ones is a phenomenon of general importance in developmental biology. It can be induced in some embryonic epithelia by appropriate culture conditions (Hay, 1978; Greenberg & Hay, 1982). It is not y ...
... Pedersen, 1978; Libbus & Hsu, 1980; Uno, 1982). The transformation of epithelial cells into mesenchymal ones is a phenomenon of general importance in developmental biology. It can be induced in some embryonic epithelia by appropriate culture conditions (Hay, 1978; Greenberg & Hay, 1982). It is not y ...
Facing extremes: archaeal surface-layer (glyco)proteins
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
The onset of pigment epithelial proliferation after retinal
... parallel to the original monolayer. Their nuclear membranes are more scalloped than those of normal RPE cell nuclei, and the usual apical-basal surface polarity is not evident. The cell surfaces are lined with many short, undifferentiated processes that sometimes interdigitate with similar processes ...
... parallel to the original monolayer. Their nuclear membranes are more scalloped than those of normal RPE cell nuclei, and the usual apical-basal surface polarity is not evident. The cell surfaces are lined with many short, undifferentiated processes that sometimes interdigitate with similar processes ...
Growth of a Tryptophanase-producing Thermophile
... tryptophanase-producing organisms in the culture was determined by detecting indole formation with Kovacs' reagent (Smibert & Krieg, 1981). Phenol formation in Tyr-PEP medium was detected with 4-aminoantipirine (Lacoste et al., 1959). A culture derived from compost from Hiroshima Prefecture, which h ...
... tryptophanase-producing organisms in the culture was determined by detecting indole formation with Kovacs' reagent (Smibert & Krieg, 1981). Phenol formation in Tyr-PEP medium was detected with 4-aminoantipirine (Lacoste et al., 1959). A culture derived from compost from Hiroshima Prefecture, which h ...
The functional diversity of essential genes required for mammalian
... Essential genes that cause lethality early in cardiac development, disrupting the processes of induction, migration of cardiac progenitors to the cardiac crescent, and heart field formation, represent a small and functionally homogeneous group with respect to their biochemical function. This group o ...
... Essential genes that cause lethality early in cardiac development, disrupting the processes of induction, migration of cardiac progenitors to the cardiac crescent, and heart field formation, represent a small and functionally homogeneous group with respect to their biochemical function. This group o ...
Link
... the early born dI4 and dI5 populations in terrestrial vertebrates (Gross et al., 2002; Mizuguchi et al., 2006; Muller et al., 2002; Wildner et al., 2006). Although the majority of dorsal cell types differentiate as sensory interneurons and sensory-relay neurons, some appear to make cellular contribu ...
... the early born dI4 and dI5 populations in terrestrial vertebrates (Gross et al., 2002; Mizuguchi et al., 2006; Muller et al., 2002; Wildner et al., 2006). Although the majority of dorsal cell types differentiate as sensory interneurons and sensory-relay neurons, some appear to make cellular contribu ...
Nucleosome depletion at yeast terminators is not
... nucleosome-remodeling activities (and perhaps transcriptional initiation) also make an important contribution to nucleosome depletion at promoters. In contrast, the correlation between in vitro and in vivo data is higher for terminators than promoters, leading to the suggestion that intrinsic histon ...
... nucleosome-remodeling activities (and perhaps transcriptional initiation) also make an important contribution to nucleosome depletion at promoters. In contrast, the correlation between in vitro and in vivo data is higher for terminators than promoters, leading to the suggestion that intrinsic histon ...
The Cell Membrane
... The nucleus contains DNA, the genetic instructions within chromosomes. The instructions tell how to synthesize the proteins that determine cell structure and function. Chromosomes also contain various proteins that control expression of the genetic information. ...
... The nucleus contains DNA, the genetic instructions within chromosomes. The instructions tell how to synthesize the proteins that determine cell structure and function. Chromosomes also contain various proteins that control expression of the genetic information. ...
Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning
... trigeminal (V) and facial (VII) motor nuclei by retrograde DiO and DiI labelling, respectively, of 10.5 dpc WT (Fig. 3A-C) and Hoxa-2−/− (Fig. 3D-I; and data not shown) embryos. In WT embryos, DiI injection at the VIIth nerve exit point in r4 labels neurons only in r4 and r5 (Fig. 3A), whereas DiO i ...
... trigeminal (V) and facial (VII) motor nuclei by retrograde DiO and DiI labelling, respectively, of 10.5 dpc WT (Fig. 3A-C) and Hoxa-2−/− (Fig. 3D-I; and data not shown) embryos. In WT embryos, DiI injection at the VIIth nerve exit point in r4 labels neurons only in r4 and r5 (Fig. 3A), whereas DiO i ...
Functional Analysis of Whole Cell Currents From Hair Cells of the
... JAY M. GOLDBERG1 AND ALAN M. BRICHTA2 Departments of 1Neurobiology, Pharmacology, and Physiology and of 2Otolaryngology-Head and Neck Surgery, ...
... JAY M. GOLDBERG1 AND ALAN M. BRICHTA2 Departments of 1Neurobiology, Pharmacology, and Physiology and of 2Otolaryngology-Head and Neck Surgery, ...
Salicylic Acid Regulates Plasmodesmata Closure
... 2011). PDLP5 is a type I transmembrane protein sharing structural and a minimal sequence homology to seven other PD-located proteins (PDLPs) (Thomas et al., 2008). Using immunolocalization in combination with correlative electron microscopy, we mapped the central cavity region as the subdomain of PD ...
... 2011). PDLP5 is a type I transmembrane protein sharing structural and a minimal sequence homology to seven other PD-located proteins (PDLPs) (Thomas et al., 2008). Using immunolocalization in combination with correlative electron microscopy, we mapped the central cavity region as the subdomain of PD ...
Intravenously-Delivered Mesenchymal Stem Cells: Systemic Anti
... To determine in vivo distribution, human MSCs (1x106/mouse, n=12), radioactively or fluorescentlylabeled, were administered intravenously to mice 24h after AMI or to control mice without AMI. Radiolabeled human MSCs were detected in all measured organs at 24h post injection (Figure 1A), showing high ...
... To determine in vivo distribution, human MSCs (1x106/mouse, n=12), radioactively or fluorescentlylabeled, were administered intravenously to mice 24h after AMI or to control mice without AMI. Radiolabeled human MSCs were detected in all measured organs at 24h post injection (Figure 1A), showing high ...
Dynamic Organization of DNA Replication in Mammalian Cell Nuclei
... specific time points during S-phase following cell synchronization allows for the precise temporal localization of DNA replication sites . In this study five different patterns of DNA replication have been identified and their timing during S-phase has been determined . In addition, the three-dimens ...
... specific time points during S-phase following cell synchronization allows for the precise temporal localization of DNA replication sites . In this study five different patterns of DNA replication have been identified and their timing during S-phase has been determined . In addition, the three-dimens ...
Characterization of chloroplasts in pavement cells of Arabidopsis
... chloroplasts (MCC). In the jigsaw shaped pavement cells in expanded cotyledons and leaves of Arabidopsis, 9 to 15 PCC are found per cell, while a single mesophyll cell may contain over 120 chloroplasts (Pyke and Leech, 1992, 1994; Barton et al., 2016). PCC are photosynthetically active and show clea ...
... chloroplasts (MCC). In the jigsaw shaped pavement cells in expanded cotyledons and leaves of Arabidopsis, 9 to 15 PCC are found per cell, while a single mesophyll cell may contain over 120 chloroplasts (Pyke and Leech, 1992, 1994; Barton et al., 2016). PCC are photosynthetically active and show clea ...
secretory immunoglobulin A triggers distinct intestinal epithelial cell
... distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. ...
... distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. ...
Differential deployment of paralogous Wnt genes in the mouse and
... (Huminiecki and Heldin 2010). The Wnt gene family was among the most highly expanded. Ohno proposed that expression divergence is the first step in the divergence of duplicate genes (Ohno 1970). Gene duplicates can be retained if they diverge to acquire new functions (neofunctionalization), includin ...
... (Huminiecki and Heldin 2010). The Wnt gene family was among the most highly expanded. Ohno proposed that expression divergence is the first step in the divergence of duplicate genes (Ohno 1970). Gene duplicates can be retained if they diverge to acquire new functions (neofunctionalization), includin ...
Expression of and Cytokine Activation by Eschevichia coli Curi
... age of LPSs in patients with sepsis [7– 9], suggesting that other components of gram-negative bacteria may play a role in the induction and pathology of septic shock. Curli are a novel class of bacterial surface structures, expressed in both Escherichia coli and Salmonella, that are characterized by ...
... age of LPSs in patients with sepsis [7– 9], suggesting that other components of gram-negative bacteria may play a role in the induction and pathology of septic shock. Curli are a novel class of bacterial surface structures, expressed in both Escherichia coli and Salmonella, that are characterized by ...
Shifts, Aneuploidy, and Loss of Heterozygosity Rapid Mechanisms
... Advanced Online Article. Cite this article as Cold Spring Harb Perspect Med doi: 10.1101/cshperspect.a019604 ...
... Advanced Online Article. Cite this article as Cold Spring Harb Perspect Med doi: 10.1101/cshperspect.a019604 ...
ExamplePoster3 - Bridgewater College
... FACTORS INFLUENCING THE SYNTHESIS OF POLYHYDROXYBUTYRATE DEPOLYMERASE IN STREPTOMYCES SP. 5A Matthew B. Persinger, Matthew Shull, and Stephen F. Baron, Biology Dept., Bridgewater College, Bridgewater, VA 22812 ...
... FACTORS INFLUENCING THE SYNTHESIS OF POLYHYDROXYBUTYRATE DEPOLYMERASE IN STREPTOMYCES SP. 5A Matthew B. Persinger, Matthew Shull, and Stephen F. Baron, Biology Dept., Bridgewater College, Bridgewater, VA 22812 ...
Selection of Shigella flexneri candidate virulence genes
... 614 C. Bartoleschi et al. Shigella spp. survive and proliferate within host cytoplasms in which they induce many biochemical reactions of cells undergoing metabolic stress (Mantis et al., 1996). Nevertheless, while much is known about plasmidencoded proteins involved in the secretory machinery, ent ...
... 614 C. Bartoleschi et al. Shigella spp. survive and proliferate within host cytoplasms in which they induce many biochemical reactions of cells undergoing metabolic stress (Mantis et al., 1996). Nevertheless, while much is known about plasmidencoded proteins involved in the secretory machinery, ent ...