Small molecules that regulate lifespan
... The hormesis hypothesis of CR, therefore, is based on the idea that low calorie intake is in itself a mild stress, which invokes a general stress response that promotes better health and longer life. This theory explains why CR animals are more resistant to a broad array of stresses and fits with pr ...
... The hormesis hypothesis of CR, therefore, is based on the idea that low calorie intake is in itself a mild stress, which invokes a general stress response that promotes better health and longer life. This theory explains why CR animals are more resistant to a broad array of stresses and fits with pr ...
The ABCs of plasmid replication and segregation
... downstream of repA (see the figure, part a). This oriR1 site contains binding sites for RepA flanked by a DnaA box at one end and three AT‑rich repeats at the other (see the figure, part b). DnaA is not essential for replication of this plasmid, but seems to have an accessory role. DNA loop formatio ...
... downstream of repA (see the figure, part a). This oriR1 site contains binding sites for RepA flanked by a DnaA box at one end and three AT‑rich repeats at the other (see the figure, part b). DnaA is not essential for replication of this plasmid, but seems to have an accessory role. DNA loop formatio ...
Small molecules that regulate lifespan: evidence for xenohormesis
... The hormesis hypothesis of CR, therefore, is based on the idea that low calorie intake is in itself a mild stress, which invokes a general stress response that promotes better health and longer life. This theory explains why CR animals are more resistant to a broad array of stresses and fits with pr ...
... The hormesis hypothesis of CR, therefore, is based on the idea that low calorie intake is in itself a mild stress, which invokes a general stress response that promotes better health and longer life. This theory explains why CR animals are more resistant to a broad array of stresses and fits with pr ...
ERRATUM
... (Huminiecki and Heldin 2010). The Wnt gene family was among the most highly expanded. Ohno proposed that expression divergence is the first step in the divergence of duplicate genes (Ohno 1970). Gene duplicates can be retained if they diverge to acquire new functions (neofunctionalization), includin ...
... (Huminiecki and Heldin 2010). The Wnt gene family was among the most highly expanded. Ohno proposed that expression divergence is the first step in the divergence of duplicate genes (Ohno 1970). Gene duplicates can be retained if they diverge to acquire new functions (neofunctionalization), includin ...
- RichardWheeler.net
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
... Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasitesdTrypanosoma brucei and Leishmania mexicana. We describe ...
Mechanisms Shaping the Membranes of Cellular Organelles
... membrane curvature in intracellular membranes is generated by proteins. Nevertheless, lipids play an important role, modifying and facilitating the function of curvature-generating proteins in decisive ways (Devaux et al. 2008). Proteins could cause curvature indirectly by generating lipid asymmetry ...
... membrane curvature in intracellular membranes is generated by proteins. Nevertheless, lipids play an important role, modifying and facilitating the function of curvature-generating proteins in decisive ways (Devaux et al. 2008). Proteins could cause curvature indirectly by generating lipid asymmetry ...
Facing extremes: archaeal surface-layer (glyco)proteins
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
... S-layer glycoproteins (Konrad & Eichler, 2002). A combination of pulse–chase radiolabelling and subcellular fractionation approaches has shown that lipid modification of the Haloferax volcanii S-layer glycoprotein only occurs once the S-layer glycoprotein has been delivered across the plasma membran ...
Functional analysis of organophosphorus hydrolase variants with high degradation
... been isolated from Agrobacterium radiobacter (Horne et al., 2002). Subtle changes in substrate specificity have been reported for OPDA, which exhibits higher kcat values for substrates with shorter side chains such as methyl parathion, suggesting that activity and substrate specificity could be alte ...
... been isolated from Agrobacterium radiobacter (Horne et al., 2002). Subtle changes in substrate specificity have been reported for OPDA, which exhibits higher kcat values for substrates with shorter side chains such as methyl parathion, suggesting that activity and substrate specificity could be alte ...
Passive transfer of anti-herpes simplex virus type 2
... membranes of HSV-2 infected corneal epithelial cells isolated from excised corneas was substantially less (P < 0.005) than that detected on HSV-1 infected cells at both 24 and 48 hours postinfection. This antigenic difference was not due to the failure of HSV-2 to replicate in corneal epithelial cel ...
... membranes of HSV-2 infected corneal epithelial cells isolated from excised corneas was substantially less (P < 0.005) than that detected on HSV-1 infected cells at both 24 and 48 hours postinfection. This antigenic difference was not due to the failure of HSV-2 to replicate in corneal epithelial cel ...
Keystone- Biology Curriculum
... breeding, gene splicing, cloning, genetically modified organisms, gene therapy) ...
... breeding, gene splicing, cloning, genetically modified organisms, gene therapy) ...
Mast cells and basophils in acquired immunity
... from mice that have been 'knocked out' for mouse mast cell protease-1 (mMCP-1) have been reported27. However, C57BL/6 mice have been found to lack mMCP-7 (a tryptase), mice with certain mutations at the mi locus have significantly decreased expression of mMCP-4, mMCP-5 and mMCP-62Si19, and NDST-2"^ ...
... from mice that have been 'knocked out' for mouse mast cell protease-1 (mMCP-1) have been reported27. However, C57BL/6 mice have been found to lack mMCP-7 (a tryptase), mice with certain mutations at the mi locus have significantly decreased expression of mMCP-4, mMCP-5 and mMCP-62Si19, and NDST-2"^ ...
Early development of the zebrafish pronephros and analysis of
... pronephric tubules. double bubble was analyzed as a representative of mutations that perturb the entire length of the pronephros and body axis curvature. Cyst formation begins in the glomerulus at 40 hpf at the time when glomerular filtration is established suggesting a defect associated with the on ...
... pronephric tubules. double bubble was analyzed as a representative of mutations that perturb the entire length of the pronephros and body axis curvature. Cyst formation begins in the glomerulus at 40 hpf at the time when glomerular filtration is established suggesting a defect associated with the on ...
Integration of TP53, DREAM, MMB-FOXM1 and RB
... revealed that many genes were commonly regulated across cell types and treatments. Proximal TP53 binding is associated with activation of 311 target genes TP53 itself is the central transcription factor mediating TP53-dependent gene regulation (1,2). To assess the role of TP53 in regulating gene exp ...
... revealed that many genes were commonly regulated across cell types and treatments. Proximal TP53 binding is associated with activation of 311 target genes TP53 itself is the central transcription factor mediating TP53-dependent gene regulation (1,2). To assess the role of TP53 in regulating gene exp ...
Contributions by members of the TGFbeta superfamily to lens
... showed that signaling through this receptor is essential for normal lens development. Lenses lacking Alk3 were smaller than normal, with thin epithelial layers. The fiber cells of Alk3 null lenses became vacuolated and degenerated within the first week after birth. Lenses lacking Alk3 function were ...
... showed that signaling through this receptor is essential for normal lens development. Lenses lacking Alk3 were smaller than normal, with thin epithelial layers. The fiber cells of Alk3 null lenses became vacuolated and degenerated within the first week after birth. Lenses lacking Alk3 function were ...
Chemical Microscopy Applied to Biological Systems
... They then investigated the binding properties of five anticancer drugs that were studied in the diploid cells using acridine orange as a fluorescent probe with a high-resolution Hadamard transform fluorescence imaging microscope. McCool et al. discuss measuring the expression of SOS genes, which are ...
... They then investigated the binding properties of five anticancer drugs that were studied in the diploid cells using acridine orange as a fluorescent probe with a high-resolution Hadamard transform fluorescence imaging microscope. McCool et al. discuss measuring the expression of SOS genes, which are ...
118 - University of Oxford
... Hensen’s node and the anterior portion of the streak. Brachyury (Ch-T) genes are expressed in forming mesoderm in response to inducing factors and at stage XII in a broad arc in the posterior epiblast. These gene expression patterns suggest that primitive streak formation can be regulated by gradien ...
... Hensen’s node and the anterior portion of the streak. Brachyury (Ch-T) genes are expressed in forming mesoderm in response to inducing factors and at stage XII in a broad arc in the posterior epiblast. These gene expression patterns suggest that primitive streak formation can be regulated by gradien ...
Fluorescent properties of c-type cytochromes
... it possible to detect reduced cytochromes in individual cells with a fluorescent microscope or within cells in a biofilm with fibre optics. There may be practical applications for this approach, not only in the analysis of Geobacter populations in environments, such as the subsurface or on energy-ha ...
... it possible to detect reduced cytochromes in individual cells with a fluorescent microscope or within cells in a biofilm with fibre optics. There may be practical applications for this approach, not only in the analysis of Geobacter populations in environments, such as the subsurface or on energy-ha ...
The Activity of a Developmentally Regulated Cysteine Proteinase Is
... of cyst wall constituents to the plasma membrane for release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and charac ...
... of cyst wall constituents to the plasma membrane for release and extracellular organization. Nevertheless, little is known about the molecular events related to cyst wall biogenesis in Giardia. Among the components of the cyst wall there are two proteins that we have previously identified and charac ...
What makes the bacteriophage λ Red system useful for genetic
... progeny phage is estimated to undergo approximately one exchange in its lineage [3], despite a chromosome length of less than 50 kb. The V-induced `hyper-rec' state is not, in itself, harmful to the bacterium. It can be induced, either permanently or transiently, in the absence of other phage proces ...
... progeny phage is estimated to undergo approximately one exchange in its lineage [3], despite a chromosome length of less than 50 kb. The V-induced `hyper-rec' state is not, in itself, harmful to the bacterium. It can be induced, either permanently or transiently, in the absence of other phage proces ...
Insights into 5-Lipoxygenase Active Site and Catalysis
... back then I had perhaps only a very vague idea of what that really entails. I think it was fed into my head by mother, even though completely unintentionally, when she used to call me her ‘scientist daughter’. And the reason for that was my unending fascination with screwdrivers and the things you c ...
... back then I had perhaps only a very vague idea of what that really entails. I think it was fed into my head by mother, even though completely unintentionally, when she used to call me her ‘scientist daughter’. And the reason for that was my unending fascination with screwdrivers and the things you c ...
Cytochrome c Oxidase dysfunction in cancer
... electrons from one enzyme complex to another. The final electron acceptor of the mammalian respiratory chain is molecular oxygen, which is reduced to water at the catalytic site of CytcO. In mammalian cells the protein subunits of NADH dehydrogenase, the bc1 complex, CytcO and ATP synthase are encod ...
... electrons from one enzyme complex to another. The final electron acceptor of the mammalian respiratory chain is molecular oxygen, which is reduced to water at the catalytic site of CytcO. In mammalian cells the protein subunits of NADH dehydrogenase, the bc1 complex, CytcO and ATP synthase are encod ...
Transcriptionally repressed germ cells lack a
... These results confirm that mAbs H5 and H14 can recognize RNAP II LS specifically in fixed interphase nuclei of C. elegans. Occasionally, some mAbs H5 and H14 immunoreactivity was detected on condensed mitotic chromosomes of ama1(as) embryos (Fig. 3B), raising the possibility that this aspect of mAbs ...
... These results confirm that mAbs H5 and H14 can recognize RNAP II LS specifically in fixed interphase nuclei of C. elegans. Occasionally, some mAbs H5 and H14 immunoreactivity was detected on condensed mitotic chromosomes of ama1(as) embryos (Fig. 3B), raising the possibility that this aspect of mAbs ...
Transcriptionally repressed germ cells lack a subpopulation of
... These results confirm that mAbs H5 and H14 can recognize RNAP II LS specifically in fixed interphase nuclei of C. elegans. Occasionally, some mAbs H5 and H14 immunoreactivity was detected on condensed mitotic chromosomes of ama1(as) embryos (Fig. 3B), raising the possibility that this aspect of mAbs ...
... These results confirm that mAbs H5 and H14 can recognize RNAP II LS specifically in fixed interphase nuclei of C. elegans. Occasionally, some mAbs H5 and H14 immunoreactivity was detected on condensed mitotic chromosomes of ama1(as) embryos (Fig. 3B), raising the possibility that this aspect of mAbs ...
Job Sharing in the Endomembrane System: Vacuolar
... pH 6.4 versus cytosolic pH 7.4) argues that the V-PPase, a homodimer of a single polypeptide chain, plays a more important role in vacuolar acidification than the V-ATPase. Arabidopsis ...
... pH 6.4 versus cytosolic pH 7.4) argues that the V-PPase, a homodimer of a single polypeptide chain, plays a more important role in vacuolar acidification than the V-ATPase. Arabidopsis ...
Copyright Information of the Article Published Online
... entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scientific community; it was first proposed for plastids at the beginning of the 20th century, but was only accepted for mitochondria for a few years before being rejected by cell biologists. It ...
... entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scientific community; it was first proposed for plastids at the beginning of the 20th century, but was only accepted for mitochondria for a few years before being rejected by cell biologists. It ...