Auxin transport through non-hair cells sustains root
... elongation 10,11, the simplest explanation of these results is that non-hair cells affect the supply of auxin to hair cells. To predict auxin distribution in the developing epidermis we incorporated the observed AUX1 expression pattern into a computer model of auxin transport in the elongation and d ...
... elongation 10,11, the simplest explanation of these results is that non-hair cells affect the supply of auxin to hair cells. To predict auxin distribution in the developing epidermis we incorporated the observed AUX1 expression pattern into a computer model of auxin transport in the elongation and d ...
Shift in GATA3 functions, and GATA3 mutations, control progression
... normal and cancer breast cells. We performed ChIP-seq of GATA3 in normal hMEC and MCF7 cells. hMEC are a mixed myoepithelial/luminal population, only the latter of which express GATA3 [35]; MCF7 is a luminal breast cancer line, which overexpresses high levels of GATA3, and thus is a convenient line ...
... normal and cancer breast cells. We performed ChIP-seq of GATA3 in normal hMEC and MCF7 cells. hMEC are a mixed myoepithelial/luminal population, only the latter of which express GATA3 [35]; MCF7 is a luminal breast cancer line, which overexpresses high levels of GATA3, and thus is a convenient line ...
Innate response to human cytomegalovirus pathogenesis of atherosclerosis
... killing of the bacteria and that response is defective in MΦ from patients with IFN-γ receptor deficiency [23, 24]. Coxiella burnetii is an intracellular bacterium that causes the Q fever, an infective endocarditis. Patients with valvulopathy exhibited increased levels of circulating apoptotic leuko ...
... killing of the bacteria and that response is defective in MΦ from patients with IFN-γ receptor deficiency [23, 24]. Coxiella burnetii is an intracellular bacterium that causes the Q fever, an infective endocarditis. Patients with valvulopathy exhibited increased levels of circulating apoptotic leuko ...
CD133 CXCR4 colon cancer cells exhibit metastatic potential and predict poor prognosis
... Results: In human specimens, the content of CD133+CXCR4+ cells was higher in liver metastases than in primary colorectal tumors. Clonogenic and tumorigenic cells were restricted to CD133+ cells in the HCT116 cell line, with CXCR4 expression having no impact on the ‘stemness’ properties. We found tha ...
... Results: In human specimens, the content of CD133+CXCR4+ cells was higher in liver metastases than in primary colorectal tumors. Clonogenic and tumorigenic cells were restricted to CD133+ cells in the HCT116 cell line, with CXCR4 expression having no impact on the ‘stemness’ properties. We found tha ...
as a PDF
... lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6), radish (lane 7), and soybean leaf (lane 9) extracts. These results were indistinguishable from those obtained with the rabbit polyclonal anti–g-TIP peptide antibodies (Jauh et al ...
... lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6), radish (lane 7), and soybean leaf (lane 9) extracts. These results were indistinguishable from those obtained with the rabbit polyclonal anti–g-TIP peptide antibodies (Jauh et al ...
Tonoplast Intrinsic Protein Isoforms as Markers for Vacuolar Functions
... lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6), radish (lane 7), and soybean leaf (lane 9) extracts. These results were indistinguishable from those obtained with the rabbit polyclonal anti–g-TIP peptide antibodies (Jauh et al ...
... lane 1) and pea seed (lane 5) extracts. MAb351 recognized appropriate bands of g-TIP in pea root tip (Figure 1B, lane 6), radish (lane 7), and soybean leaf (lane 9) extracts. These results were indistinguishable from those obtained with the rabbit polyclonal anti–g-TIP peptide antibodies (Jauh et al ...
How plant cells explore geometry - The polarity induction and
... quantitative data on the temporal patterns of regeneration stages. Hereinafter this system was integrated into a microfluidic platform to study the impacts of chemical and geometrical stimuli during the establishment of polarity. Cell polarity and axis development are central for plant morphogenesis ...
... quantitative data on the temporal patterns of regeneration stages. Hereinafter this system was integrated into a microfluidic platform to study the impacts of chemical and geometrical stimuli during the establishment of polarity. Cell polarity and axis development are central for plant morphogenesis ...
Mochii - Salamander Genome Project
... embryonic tailbud. The above idea is also supported by the following observation. The notochord, spinal cord and muscle tissues continue to grow posteriorly in the most distal region of the normal tail with morphological similarity to the tissues in the regenerating tail. Comprehensive analysis of g ...
... embryonic tailbud. The above idea is also supported by the following observation. The notochord, spinal cord and muscle tissues continue to grow posteriorly in the most distal region of the normal tail with morphological similarity to the tissues in the regenerating tail. Comprehensive analysis of g ...
biology and medicine Embryonic stem cell differentiation
... 1979; Godin et al. 1995; Medvinsky and Dzierzak 1996; Palis et al. 1999). Detailed analysis of hematopoietic development in the early embryo strongly suggests that the programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct bl ...
... 1979; Godin et al. 1995; Medvinsky and Dzierzak 1996; Palis et al. 1999). Detailed analysis of hematopoietic development in the early embryo strongly suggests that the programs generated in these two regions are different. Hematopoietic commitment is detected first in the yolk sac, where distinct bl ...
full text pdf
... role in cellular redox regulation. Similarly to AA, changes in the GSH pool and its redox status could act as redox signals integrated into the network that regulates gene expression, including antioxidant defense genes [11,19]. Protein S-glutathionylation, a process of mixed disulfide formation bet ...
... role in cellular redox regulation. Similarly to AA, changes in the GSH pool and its redox status could act as redox signals integrated into the network that regulates gene expression, including antioxidant defense genes [11,19]. Protein S-glutathionylation, a process of mixed disulfide formation bet ...
Mitochondria Know No Boundaries: Mechanisms and Functions of
... depending on the cell type. Myosin is a fundamental protein for the organelle transfer, a process where high rates of ATP consumption are needed. Rho GTPases play an important role in organelle transfer through TNT. Miro1 and microtubules have been involved in the transfer of mitochondria upon injur ...
... depending on the cell type. Myosin is a fundamental protein for the organelle transfer, a process where high rates of ATP consumption are needed. Rho GTPases play an important role in organelle transfer through TNT. Miro1 and microtubules have been involved in the transfer of mitochondria upon injur ...
Commentary
... convincing argument for the in vivo significance of Salp15–B. burgdorferi binding could be made if the bacteria were rinsed after incubation with Salp15, before injection into mice. Without these data, one wonders whether the increased bacterial load in mice several weeks later is not the result of ...
... convincing argument for the in vivo significance of Salp15–B. burgdorferi binding could be made if the bacteria were rinsed after incubation with Salp15, before injection into mice. Without these data, one wonders whether the increased bacterial load in mice several weeks later is not the result of ...
PDF
... 1997; Knezevic et al., 1998), reveal many commonalities between these two processes in several vertebrate species. Additionally, mutation studies show that several genes, including members of the Wnt gene family and brachyury, have a role during both primitive streak and tail bud outgrowth in mouse ...
... 1997; Knezevic et al., 1998), reveal many commonalities between these two processes in several vertebrate species. Additionally, mutation studies show that several genes, including members of the Wnt gene family and brachyury, have a role during both primitive streak and tail bud outgrowth in mouse ...
Targeting Membrane-Bound Viral RNA Synthesis Reveals
... Specifically for positive-stranded RNA viruses, accumulating knowledge concerning the involvement, rearrangement and requirement of cellular membranes for RNA synthesis specify the establishment of the viral replicase complex at host cell-derived membranes as an evolutionary conserved and essential ...
... Specifically for positive-stranded RNA viruses, accumulating knowledge concerning the involvement, rearrangement and requirement of cellular membranes for RNA synthesis specify the establishment of the viral replicase complex at host cell-derived membranes as an evolutionary conserved and essential ...
Prediction of protease substrates using sequence
... and all human proteome octapeptides with Asp in the fourth position were processed for the application step (Section 3). Comparative models were generated by the automated modeling pipeline ModPipe (Pieper et al., 2009), and only good quality models [those predicted to have >80% of their C-α atoms w ...
... and all human proteome octapeptides with Asp in the fourth position were processed for the application step (Section 3). Comparative models were generated by the automated modeling pipeline ModPipe (Pieper et al., 2009), and only good quality models [those predicted to have >80% of their C-α atoms w ...
Localised axial progenitor cell populations in the avian tail bud are
... 1997; Knezevic et al., 1998), reveal many commonalities between these two processes in several vertebrate species. Additionally, mutation studies show that several genes, including members of the Wnt gene family and brachyury, have a role during both primitive streak and tail bud outgrowth in mouse ...
... 1997; Knezevic et al., 1998), reveal many commonalities between these two processes in several vertebrate species. Additionally, mutation studies show that several genes, including members of the Wnt gene family and brachyury, have a role during both primitive streak and tail bud outgrowth in mouse ...
Pathogenicity and Commensalism Recognition Contribute to Fungal
... Australia). Mannan and D-mannose were from Sigma-Aldrich. CA-mannan and glucan were a kind gift from Prof. David Williams and were previously isolated (15, 16). The following Abs were used: mAb AZN-D1, AZN-D2 and AZN-D3 anti-DC-SIGN (17), NKI-L19, and anti-b2integrins. mAb anti-MMR and mAb DCN46 ant ...
... Australia). Mannan and D-mannose were from Sigma-Aldrich. CA-mannan and glucan were a kind gift from Prof. David Williams and were previously isolated (15, 16). The following Abs were used: mAb AZN-D1, AZN-D2 and AZN-D3 anti-DC-SIGN (17), NKI-L19, and anti-b2integrins. mAb anti-MMR and mAb DCN46 ant ...
Expression of Multiple Artificial MicroRNAs from a Chicken miRNA126-Based Lentiviral Vector
... evaluated by dual luciferase assays following either transient transfection or stable integration of the lentiviral vector in chicken embryonic fibroblast DF-1 cells. In the transient transfection assay, the miR30-NP lentiviral vector and psicheck-2 dual luciferase reporter plasmid, in which the NP ...
... evaluated by dual luciferase assays following either transient transfection or stable integration of the lentiviral vector in chicken embryonic fibroblast DF-1 cells. In the transient transfection assay, the miR30-NP lentiviral vector and psicheck-2 dual luciferase reporter plasmid, in which the NP ...
Signaling networks that regulate muscle development: Lessons from
... 2002). Thus, Ntl and Spt are essential together for expression of myogenic genes (Amacher et al. 2002; Goering et al. 2003). Hedgehog signaling in muscle development Hedgehog (Hh) is a secreted signaling protein that acts through at least two proteins, Patched (Ptc) and Smoothened (Smo). Ptc negativ ...
... 2002). Thus, Ntl and Spt are essential together for expression of myogenic genes (Amacher et al. 2002; Goering et al. 2003). Hedgehog signaling in muscle development Hedgehog (Hh) is a secreted signaling protein that acts through at least two proteins, Patched (Ptc) and Smoothened (Smo). Ptc negativ ...
Transcription • Transcription factors • Chromatin • RNA polymerase II
... termination. However, substitution of Ser7 by the non– phospho-acceptor alanine in each heptapeptide has little effect on expression of endogenous or transiently transfected protein-coding genes [6,22]. By contrast, alanine substitution of Ser7 causes a marked defect in the transcription of human sn ...
... termination. However, substitution of Ser7 by the non– phospho-acceptor alanine in each heptapeptide has little effect on expression of endogenous or transiently transfected protein-coding genes [6,22]. By contrast, alanine substitution of Ser7 causes a marked defect in the transcription of human sn ...
Transcription factories
... of many genes. This regulation can now be monitored at thousands of genes simultaneously using high throughput microarrays and next-generation sequencing. These technologies have revealed temporally coordinated changes in the transcription levels of many genes in response to developmental or environ ...
... of many genes. This regulation can now be monitored at thousands of genes simultaneously using high throughput microarrays and next-generation sequencing. These technologies have revealed temporally coordinated changes in the transcription levels of many genes in response to developmental or environ ...
Diverse roles of the Mediator complex in plants
... eukaryotic lineages, Bourbon, (2008) showed that Mediator subunits from very diverse phyla could be identified using evolutionary conserved signature sequence motifs (SSMs). Using these analyses, Bourbon (2008) identified all but two Mediator subunits in plants and all of the human subunits in the d ...
... eukaryotic lineages, Bourbon, (2008) showed that Mediator subunits from very diverse phyla could be identified using evolutionary conserved signature sequence motifs (SSMs). Using these analyses, Bourbon (2008) identified all but two Mediator subunits in plants and all of the human subunits in the d ...
Pascale G. Charest and Michel Bouvier Activation
... presence of 100 M 2-bromopalmitic acid (2-BrP) (Fluka Chemie) or 4 h with 30 M tunicamycin at 37 °C. After metabolic labeling, V2Rs were purified by immunoprecipitation using the AS435 antibody raised against the carboxyl-terminal portion of the human V2R (peptide ARG29, ARGRTPPSLGPQDESCTTASSSLAKD ...
... presence of 100 M 2-bromopalmitic acid (2-BrP) (Fluka Chemie) or 4 h with 30 M tunicamycin at 37 °C. After metabolic labeling, V2Rs were purified by immunoprecipitation using the AS435 antibody raised against the carboxyl-terminal portion of the human V2R (peptide ARG29, ARGRTPPSLGPQDESCTTASSSLAKD ...
The role of active oxygen species in plant signal transduction
... pathogen and plant cells. Killing of the plant cells surrounding the infection site inhibits spreading of a (biotrophic) pathogen [24]. (ii) Hydrogen peroxide can serve as a substrate in peroxidative cross-linking reactions of lignin precursors and induce cross-linking of cell wall proteins. A reinf ...
... pathogen and plant cells. Killing of the plant cells surrounding the infection site inhibits spreading of a (biotrophic) pathogen [24]. (ii) Hydrogen peroxide can serve as a substrate in peroxidative cross-linking reactions of lignin precursors and induce cross-linking of cell wall proteins. A reinf ...
AA-tRNA - Studentportalen
... initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. eIF4F is composed of 4E, 4G and 4A. 3.) The binding o ...
... initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. eIF4F is composed of 4E, 4G and 4A. 3.) The binding o ...