Concerted Assembly and Cloning of Multiple DNA Segments Using
... To examine whether the assembled fusion proteins were functional, HEK293 cells were transfected with three representative Expression Clones as follows: CMV promoter-B4-NLS-B1.1eGFP-B2.1-poly adenylation signal; CMV promoter-B1.1-arrestin-B2.1-eGFP-B3-poly(A); and CMV promoter-B4Transferrin receptor ...
... To examine whether the assembled fusion proteins were functional, HEK293 cells were transfected with three representative Expression Clones as follows: CMV promoter-B4-NLS-B1.1eGFP-B2.1-poly adenylation signal; CMV promoter-B1.1-arrestin-B2.1-eGFP-B3-poly(A); and CMV promoter-B4Transferrin receptor ...
Tissues Produced by Meristems
... Lateral Meristems - Produce secondary tissues that increase the girth of roots and stems (= secondary growth) • Vascular Cambium - Produces tissues that ...
... Lateral Meristems - Produce secondary tissues that increase the girth of roots and stems (= secondary growth) • Vascular Cambium - Produces tissues that ...
Genetics, Development and Cell Biology (GDCB) Iowa State University – 2013-2014 1
... Broad overview of bioinformatics with a significant problem-solving component, including hands-on practice using computational tools to solve a variety of biological problems. Topics include: database searching, sequence alignment, gene prediction, RNA and protein structure prediction, construction ...
... Broad overview of bioinformatics with a significant problem-solving component, including hands-on practice using computational tools to solve a variety of biological problems. Topics include: database searching, sequence alignment, gene prediction, RNA and protein structure prediction, construction ...
The Structure of MYB Proteins
... – Some are clearly transcription factors (from circadian clock to root hair formation) ...
... – Some are clearly transcription factors (from circadian clock to root hair formation) ...
LexA-GAL4 Activates Transcription from a Downstream Site
... 5' ends as those gene rated by the wild-type promoter, but are present at an unexpectedly low level. LexA-GAL4 activates transcription, at further reduced efficiency, when its binding site lies downstream of the normal transcription start. In this case, we have not yet determined the location of the ...
... 5' ends as those gene rated by the wild-type promoter, but are present at an unexpectedly low level. LexA-GAL4 activates transcription, at further reduced efficiency, when its binding site lies downstream of the normal transcription start. In this case, we have not yet determined the location of the ...
The Plasma Membrane
... • 3. Proteins that are embedded in or that lie on the bilayer. • 4. Short carbohydrate chains on the cell surface, collectively called the glycocalyx, that function in cell adhesion and as binding sites on proteins. ...
... • 3. Proteins that are embedded in or that lie on the bilayer. • 4. Short carbohydrate chains on the cell surface, collectively called the glycocalyx, that function in cell adhesion and as binding sites on proteins. ...
Is Evolutionary Theory Central to Molecular Cell Biology?
... Signal transducer and activator of transcription (STAT) proteins are typically latent in the cytoplasm until activation by extracellular signaling proteins (Levy & Darnell, 2002). The signaling proteins that activate STATs include cytokines, growth factors, and even some simple peptides. These signa ...
... Signal transducer and activator of transcription (STAT) proteins are typically latent in the cytoplasm until activation by extracellular signaling proteins (Levy & Darnell, 2002). The signaling proteins that activate STATs include cytokines, growth factors, and even some simple peptides. These signa ...
Syringolin Reprograms Wheat to Undergo
... haustorium formation, and development of secondary hyphae were significantly suppressed on syringolin A–treated leaves (data not shown). In summary, these observations argue against a direct effect of syringolin A on early fungal development but do not rule out a fungitoxic activity affecting stages ...
... haustorium formation, and development of secondary hyphae were significantly suppressed on syringolin A–treated leaves (data not shown). In summary, these observations argue against a direct effect of syringolin A on early fungal development but do not rule out a fungitoxic activity affecting stages ...
Mechanisms of Glucocorticoid-Regulated Gene Transcription
... can effectively dimerize [9]. The variable spacing for these nGREs suggests that dimerization might not be required at these nGREs and accordingly structural studies suggest monomeric GR-binding to the half sites (Fig. 3.3) [62]. Together these studies suggest that GR is able to interact directly wi ...
... can effectively dimerize [9]. The variable spacing for these nGREs suggests that dimerization might not be required at these nGREs and accordingly structural studies suggest monomeric GR-binding to the half sites (Fig. 3.3) [62]. Together these studies suggest that GR is able to interact directly wi ...
High-throughput screens and selections of enzyme
... higher than other alkaline phosphatase antibodies generated by immunization with a transition state analogue. This high catalytic efficiency was mainly the result of an increase in kcat value, demonstrating the importance of selection for multiple turnovers. Another example regards the selection of ...
... higher than other alkaline phosphatase antibodies generated by immunization with a transition state analogue. This high catalytic efficiency was mainly the result of an increase in kcat value, demonstrating the importance of selection for multiple turnovers. Another example regards the selection of ...
Interleukin 1, Interleukin 6, Tumor Necrosis
... 24 h before lethal doses of TNF, bacteria, or some inflammatory agents has been demonstrated in various in vivo and in vitro models. The mechanisms which protect target cells from being killed by TNF remain unclear. Previous reports (13-15) have shown that activation of PKC (16) plays a role in the ...
... 24 h before lethal doses of TNF, bacteria, or some inflammatory agents has been demonstrated in various in vivo and in vitro models. The mechanisms which protect target cells from being killed by TNF remain unclear. Previous reports (13-15) have shown that activation of PKC (16) plays a role in the ...
Alvin_20.385_Presentation
... limited (CRP7/Om7) Bases that do not contact CRP can have impact in binding affinity Possible to create orthogonal pathways with small number of mutations ...
... limited (CRP7/Om7) Bases that do not contact CRP can have impact in binding affinity Possible to create orthogonal pathways with small number of mutations ...
Laser-Micropipet Combination for Single-Cell Analysis
... prior to sampling. For nonadherent cells, the use of electroosmotic flow (EOF) to move the cell into the capillary inlet may impact the cellular process to be measured. A large body of literature exists on the biological effects of electric fields.16-19 Electric fields as low as 1-15 V/cm can cause ...
... prior to sampling. For nonadherent cells, the use of electroosmotic flow (EOF) to move the cell into the capillary inlet may impact the cellular process to be measured. A large body of literature exists on the biological effects of electric fields.16-19 Electric fields as low as 1-15 V/cm can cause ...
ochromonas malhamensis
... acid phosphatase (E .C . 3 .1 .3 .2 orthophosphoric monoester phosphohydrolase) and acid ribonuclease (E .C . 2 .7.7 .16 ribonucleate nucleotido-2'-transferase) were done on Ochromonas cultures in the same experimental treatments and under identical assay conditions as the cytochemical study . Durin ...
... acid phosphatase (E .C . 3 .1 .3 .2 orthophosphoric monoester phosphohydrolase) and acid ribonuclease (E .C . 2 .7.7 .16 ribonucleate nucleotido-2'-transferase) were done on Ochromonas cultures in the same experimental treatments and under identical assay conditions as the cytochemical study . Durin ...
Non-Flagellar Swimming in Marine Synechococcus
... manipulation of Synechococcus has made it possible to address the problem at the molecular level (Brahamsha, 1996b). In the course of experiments designed to detect cell-surface components that may be involved in swimming, a major polypeptide of 130 kDa was identified whose decrease in abundance fol ...
... manipulation of Synechococcus has made it possible to address the problem at the molecular level (Brahamsha, 1996b). In the course of experiments designed to detect cell-surface components that may be involved in swimming, a major polypeptide of 130 kDa was identified whose decrease in abundance fol ...
Cellular Senescence and the Biology of Aging, Disease, and Frailty
... tissue dysfunction. Importantly, we observed no overt side effects of senescent cell clearance in our model, even though it has been postulated that senescent cells might enhance certain types of tissue repair. The translation of these findings to humans is contingent upon the ability to specificall ...
... tissue dysfunction. Importantly, we observed no overt side effects of senescent cell clearance in our model, even though it has been postulated that senescent cells might enhance certain types of tissue repair. The translation of these findings to humans is contingent upon the ability to specificall ...
Yeast longevity and aging—the mitochondrial connection
... operation we had adduced some time ago. The studies of age asymmetry in yeast have implications for stem cell aging. The renewal of many tissues requires the function of stem cells. This renewal is critical for maintenance of function as the organism ages. However, it is essential that the cells tha ...
... operation we had adduced some time ago. The studies of age asymmetry in yeast have implications for stem cell aging. The renewal of many tissues requires the function of stem cells. This renewal is critical for maintenance of function as the organism ages. However, it is essential that the cells tha ...
Adherent Neural Stem (NS) Cells from Fetal and
... symmetrical division offers critical opportunities for experimentation, allowing identification of factors and genes acting directly on the stem cells to regulate self-renewal and commitment (Smith 2001; Gottlieb 2002). Clonal and adherent expansion also enables efficient genome engineering and geneti ...
... symmetrical division offers critical opportunities for experimentation, allowing identification of factors and genes acting directly on the stem cells to regulate self-renewal and commitment (Smith 2001; Gottlieb 2002). Clonal and adherent expansion also enables efficient genome engineering and geneti ...
Patients Cell Development Reminiscent to SCID IL
... poorly understood. Cytokines have been presumed to play an important role during T cell development, but thus far formal proof has been missing. Most cytokine-knockout mice show apparently normal intrathymic T cell development. The exception to this is IL-7, given that both IL-7 and IL-7Ra knockout ...
... poorly understood. Cytokines have been presumed to play an important role during T cell development, but thus far formal proof has been missing. Most cytokine-knockout mice show apparently normal intrathymic T cell development. The exception to this is IL-7, given that both IL-7 and IL-7Ra knockout ...
Journal of Microbiological Methods
... microsites, which are relevant for a specific process and the corresponding organisms. Bartosch et al. (1999), for example, detected nitrifying bacteria in enrichment cultures of a wastewater treatment plant by antibodies, specific for nitrite-oxidoreductase. Also, Lin et al. (1996) used a similar a ...
... microsites, which are relevant for a specific process and the corresponding organisms. Bartosch et al. (1999), for example, detected nitrifying bacteria in enrichment cultures of a wastewater treatment plant by antibodies, specific for nitrite-oxidoreductase. Also, Lin et al. (1996) used a similar a ...
Effects of Null Mutations in the Hexokinase Genes of Saccharomyces cerevisiae on Catabolite Repression.
... inserted into a Sall site in the coding region of the HXKJ gene (29, 55). The transplacement to introduce the hxkl::LEU2+ allele onto the chromosome was performed essentially as previously described (49) by selecting for leucine prototrophy in a leu2-3,2-112 background (DBY1315). Three null mutation ...
... inserted into a Sall site in the coding region of the HXKJ gene (29, 55). The transplacement to introduce the hxkl::LEU2+ allele onto the chromosome was performed essentially as previously described (49) by selecting for leucine prototrophy in a leu2-3,2-112 background (DBY1315). Three null mutation ...
The Role of the Leukemia Inhibitory Factor (LIF)
... [62]. c-MYC is a transcription factor that controls many different biological processes, such as cell proliferation, growth, differentiation and apoptosis. Moreover, c-Myc is, together with OCT3/4, KLF4, and SOX2, one of the transcription factors able to reprogram somatic cells into undifferentiated ...
... [62]. c-MYC is a transcription factor that controls many different biological processes, such as cell proliferation, growth, differentiation and apoptosis. Moreover, c-Myc is, together with OCT3/4, KLF4, and SOX2, one of the transcription factors able to reprogram somatic cells into undifferentiated ...
How signaling modalities link oogenesis to
... Follicles • Follicles activate from primordials • Grow to increase somatic cell number (hyperplasia) and support germ cell hypertrophy • Acquire gonadotropin sensitivity along with steroidogenic capacity • Acquire ovulation competency for both the mural and cumulus compartments ...
... Follicles • Follicles activate from primordials • Grow to increase somatic cell number (hyperplasia) and support germ cell hypertrophy • Acquire gonadotropin sensitivity along with steroidogenic capacity • Acquire ovulation competency for both the mural and cumulus compartments ...
Endocrine System PPT
... produced in one part of the body that travels to another part of the body (target tissue) and elicits a response. ...
... produced in one part of the body that travels to another part of the body (target tissue) and elicits a response. ...