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Sarcolemmal cholesterol and caveolin-3 dependence of - AJP
Sarcolemmal cholesterol and caveolin-3 dependence of - AJP

... Perfused heart preparation. Mice were anesthetized with 60 mg/kg pentobarbital sodium, and hearts removed and perfused as previously described (16, 37, 58). Briefly, hearts were rapidly excised into ice-cold perfusion fluid, the aorta was cannulated, and the coronary circulation was perfused in Lang ...
Overview of Eye Histology Tunica fibrosa (fibrous, supporting layer
Overview of Eye Histology Tunica fibrosa (fibrous, supporting layer

... Tectorial mem.: from spiral limbus to organ of Corti by stereocilia of hair cells; radially oriented bundles of collagens embedded in ...
Microtubule and F-actin dynamics at the division site in living
Microtubule and F-actin dynamics at the division site in living

... Microinjection of living cells We describe here aspects of in vivo dynamics of cortical MTs and F-actin in living Tradescantia stamen hair cells, observed by confocal microscopy after microinjection of either Rh-phalloidin or carboxyfluorescein-labelled tubulin. An issue that needs to be addressed a ...
PDF Datastream - Brown Digital Repository
PDF Datastream - Brown Digital Repository

Journal of Medical Microbiology
Journal of Medical Microbiology

... repair of DNA damage caused by exposure to a low pH or reactive oxygen species produced by immune cells during bacterial infections [17]. Recently it was found that H. pylori binds with high af®nity to the glycosaminoglycans such as heparin and heparan sulphate, and to heparin-dependent growth facto ...
In vitro control of neuronal polarity by
In vitro control of neuronal polarity by

... absence of neurotrophicity of GAGs previously immobilized on the culture substratum. Although the mechanisms involved in GAGs neurotrophic effects and in the separate regulation of neuronal polarity by HS and DS were not elucidated, we found that, as opposed to HS, DS was able to enhance neuronal ad ...
physiological and chemical architecture of a lobster ganglion with
physiological and chemical architecture of a lobster ganglion with

... Estimation of cell body volume. Only rough measurements of cell volume could be made on the living cell with the dissecting microscope. It was found that the manipulation required to measure the diameter of living cells under the compound microscope (cell transfers, temperature changes, etc.) result ...
Dystroglycan controls signaling of multiple hormones through
Dystroglycan controls signaling of multiple hormones through

... development in vivo is not clear. The conditional deletion of DG from the luminal epithelium of the prostate gland did not produce obvious defects of tissue development or function, but a deletion from the myoepithelial (basal epithelial) compartment of the prostate was not assessed (Esser et al., 2 ...
The proteome of the infectious bronchitis virus Beau
The proteome of the infectious bronchitis virus Beau

... unique peptide matches identified for each protein in each replicate (N – 144, 81 and 50; S – 21, 14 and 12; M – 11, 7 and 7). The fourth viral structural protein, E, the replicase proteins and the lineage-specific accessory proteins could not be identified. The total number of proteins identified i ...
A conditional mutation in Arabidopsis thaliana
A conditional mutation in Arabidopsis thaliana

... manufacturer’s manual; Roche, Indianapolis, IN, USA) with five sequencespecific digoxigenin-11-dUTP-labeled DNA probes (336 to 542 bp; see Fig. 1B, asterisks) was used to isolate 32 clones covering the 56 kb RSW4containing interval from a genomic cosmid library constructed in the binary vector pBIC2 ...
Mode Suppressed TEM Cell Design For High Frequency IC
Mode Suppressed TEM Cell Design For High Frequency IC

pdf: Wu et al. 2010
pdf: Wu et al. 2010

... manufacturer’s manual; Roche, Indianapolis, IN, USA) with five sequencespecific digoxigenin-11-dUTP-labeled DNA probes (336 to 542 bp; see Fig. 1B, asterisks) was used to isolate 32 clones covering the 56 kb RSW4containing interval from a genomic cosmid library constructed in the binary vector pBIC2 ...
The PXY-CLE41 receptor ligand pair defines a
The PXY-CLE41 receptor ligand pair defines a

... CLE41 acts in secondary growth To address the role of CLE41 in PXY signalling during secondary growth, we analysed hypocotyls in plants with altered CLE41 expression (Fig. 4A-C,E-G). Phenotypes were even more dramatic than those observed in inflorescence stems. Altering the CLE41 expression domain o ...
Introduction and Epithelial Tissues
Introduction and Epithelial Tissues

... – Epithelia are tissues that form cellular membrane surfaces by covering other tissues. – Epithelia are found lining the body (the skin), lining cavities and tubes of the body, and they form some glands (glandular epithelium). ...
A myosin inhibitor impairs auxin
A myosin inhibitor impairs auxin

... 1999), little is known about their cellular function. In most cases, the specific functions of plant myosins have still to be derived from sequence homologies with nonplant myosins of known function (Mooseker and Cheney 1996). For instance, from the sequence similarity between the tail domains of cl ...
Young Innovators 2009
Young Innovators 2009

... translocation of the hydrophilic anti-diabetic drug metformin from the blood into hepatocytes. Since many drugs are inhibitors of the OCT1 [1], this may lead to drug-drug interactions at the OCT1. The risk of drugdrug interactions may be further increased in individuals with an OCT1 protein with red ...
Constructing a Plant Cell. The Genetic Control of Root Hair
Constructing a Plant Cell. The Genetic Control of Root Hair

... begin to adopt a particular position-dependent fate? As mentioned above, it has long been known that epidermal cells at the H and N positions exhibit cytological differences within the meristematic region of the root, indicating that cell specification must begin at a relatively early stage of root ...
The cell cycle and pluripotency
The cell cycle and pluripotency

... to cause commitment of pluripotent inner cell mass cells to the trophoblast lineage [33]. In addition to its role in preventing endoreplication, geminin is present in G1 in mESCs and directly maintains the expression of the pluripotency genes Sox2, Nanog and Oct4 [34]. More recently, roles for gemin ...
Early and late endosomal compartments of Entamoeba histolytica
Early and late endosomal compartments of Entamoeba histolytica

... of the internalized macromolecules is likely to be carried out by hydrolytic enzymes, including proteases [3], which may reside in endo-lysosomal-like compartments. Endocytic processes are poorly defined in E. histolytica. It is known, however, that these amoebae can actively phagocytose large parti ...
Bacillus globigii cell size is influenced by
Bacillus globigii cell size is influenced by

Cyclin synthesis and degradation and the embryonic cell cycle
Cyclin synthesis and degradation and the embryonic cell cycle

... interphase the nuclei are intact, D N A synthesis occurs (Blow and Watson, 1987; Hutchison et al. 1987) and the activity of M PF and H I kinase are low (Murray and Kirschner, 1989); as mitosis is initiated the activity of M PF and H I kinase increases, the chromosomes condense, nuclear envelope brea ...
Weekly Lesson Plans Teacher: Alicia Penfield Class: AP Biology
Weekly Lesson Plans Teacher: Alicia Penfield Class: AP Biology

Gene transcription is coordinated with, but not dependent on, cell
Gene transcription is coordinated with, but not dependent on, cell

... transgenes. clk-1(qm30); itIs38; stIs10116 worms were grown at 20°C for over two generations before embryos were imaged at 20°C. Strains containing the temperature-sensitive alleles div-1(or148), emb16(g19) or emb-29(g52) were grown at 15°C before L4 hermaphrodites were incubated overnight at 25°C a ...
Modulation of T cell signaling by the actin cytoskeleton
Modulation of T cell signaling by the actin cytoskeleton

Seasonal Changes of Plasma Membrane H
Seasonal Changes of Plasma Membrane H

... Hoth et al., 1997; Maathuis et al., 1997). With respect to trees, numerous studies have shown that auxin is an important regulator of cambial growth (Savidge and Wareing, 1981; Little and Savidge, 1987; Lachaud, 1989; Little and Pharis, 1995; Uggla et al., 1998; Sundberg et al., 2000). However, our ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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