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J153
J153

... for the repeating distance of bipolar plates in the stack, in keeping with the US-DOE target for volumetric power density of 2 kW/L. Flow field design has been shown to play a key role in influencing the water flooding in the channels. Both serpentine and interdigitated flow fields have shown reduce ...
Simulations Suggest Information Processing Roles for the Diverse
Simulations Suggest Information Processing Roles for the Diverse

Antibiotics for research
Antibiotics for research

... scientists identifying and isolating active moieties responsible for antibacterial activity. Today antibiotics are widely used in the Life Sciences not only to eliminate contamination, but also to identify bacterial mechanisms of resistance, protein modification and DNA/RNA manipulation to develop n ...
2013 jeopardy review
2013 jeopardy review

... And neutrons added together? ...
High-throughput knockout screen in Schizosaccharomyces pombe
High-throughput knockout screen in Schizosaccharomyces pombe

... The reduction of chromosome number during meiosis is achieved by a single round of DNA replication followed by two rounds of chromosome segregation, called meiosis I and meiosis II. While the second meiotic division is similar to mitosis in that sister centromeres segregate to opposite poles, the fi ...
Cunningham_etal_2012_Experimental
Cunningham_etal_2012_Experimental

... bacterial biofilm components [17]. We found that although fixed H. erythrogramma embryos can be directly fractured for SEM observation [17,19], Thiomargarita are too fragile to section this way. We sectioned samples successfully by collection on Millipore filters and embedding in agarose. Sections o ...
Temperature-controlled US-mediated intracellular delivery of a
Temperature-controlled US-mediated intracellular delivery of a

... exposure to ultrasound can be sufficient to mediate extravasation and cellular uptake leading to an enhanced drug efficacy [10-12]. This effect has been attributed to microscopic fluid motions induced by the oscillating microbubbles in the ultrasound field (referred to as stable cavitation) that dr ...
immunohistological study of mannan polysaccharides in poplar stem
immunohistological study of mannan polysaccharides in poplar stem

... INTRODUCTION Plant cell walls not only play a key role in cell differentiation, intercellular communication and cell defense, but also provide rigidity to plant body, while giving flexibility during cell expansion. The cell walls are highly organized composites, which contain a variety of polysaccha ...
Digest Select - Moss Nutrition
Digest Select - Moss Nutrition

... protease, alpha-galactosidase). Enzyme activity is assayed according to current FCC and industry standards, i.e. not less than 85% and not more than 115% of the declared units of enzyme activity. All the microbial enzymes used in Digest Select™ are acid-stable and designed to survive the acidic cond ...
Nup153 is an M9containing mobile nucleoporin with a novel
Nup153 is an M9containing mobile nucleoporin with a novel

... 50–100 different proteins, termed nucleoporins, that assemble in multiple copies to form the complex. Biochemical, genetic and genome sequencing approaches have led to the identification of ~30 yeast nucleoporins or NPCassociated proteins, while about half as many higher eukaryotic nucleoporins have ...
AMBER PARTICLES AS LIVING PLANT CELL MARKERS IN FLOW
AMBER PARTICLES AS LIVING PLANT CELL MARKERS IN FLOW

Environmental factors that induce oxidative stress
Environmental factors that induce oxidative stress

... Electron transfers --> the oxidation of intermediary metabolites by O2 in the mitochondria . It often requires the successive transfer of H atoms or electrons, first to NAD+, then from NADH to an ubiquinone (Q), next from QH2 to ferricytochrome c and finally from ferrocytochrome c to O2. These react ...
Plasma Membrane Depolarization Induced by
Plasma Membrane Depolarization Induced by

... 2001). The mechanisms by which these external stimuli inhibit H1-ATPases are still not completely elucidated, but several pieces of evidence suggest that an increase in cytoplasmic calcium is also involved in this process (Kinoshita et al., 1995; Lino et al., 1998; Schaller and Oecking, 1999). In Ly ...
Association between cell wall-related processes and
Association between cell wall-related processes and

Membrane Topology of Cytochrome P450 2B4 in
Membrane Topology of Cytochrome P450 2B4 in

... protein concentration. Conversely, the change in surface pressure during the fast phase was found to be dependent only on the subphase concentration. Injection of P450 2B4 Δ2–27 or Δb5 into the subphase of either DPPE or DPPC monolayers (Fig. 2) caused only a slow-phase pressure increase. No fast ph ...
Full Text  - The International Journal of Developmental Biology
Full Text - The International Journal of Developmental Biology

... show" that the basic forces in organisms conform with those in inorganic nature in so far as they act by laws of necessity and blindly without consideration for any purpose, and that they are forces that were given together with the existence of matter, like the forces of physics." The latter forces ...
Molecular Definition of Distinct Cytoskeletal Structures
Molecular Definition of Distinct Cytoskeletal Structures

... o f protein kinase C (PKC-e 0 were distributed more uniformly. These loci o f cytoskeletal proteins may represent the point-like contact areas previously seen by using EM, and their formation was blocked by inhibitors o f PKC, but not by inhibitors o f protein tyrosine kinases. By contrast, all prot ...
Inductive asymmetric cell division
Inductive asymmetric cell division

PROTEINS IN NUCLEOCYTOPLASMIC INTERACTIONS III
PROTEINS IN NUCLEOCYTOPLASMIC INTERACTIONS III

Designed Ankyrin Repeat Proteins (DARPins)
Designed Ankyrin Repeat Proteins (DARPins)

... on the efficiency of targeting was systematically investigated. DARPins are very small (molecular weight, MW 15–18kDa) yet can easily be PEGylated (see Sections 3.3 and 3.8). For a DARPin conjugated to a PEG molecule with a nominal MW of 20kDa, the hydrodynamic properties correspond to a MW of about ...
Senescence-Associated Vacuoles, a Specific Lytic Compartment for
Senescence-Associated Vacuoles, a Specific Lytic Compartment for

... membrane bound vesicles detected in the cytosol of leaf cells through transmission electron microscopy [33]. Their numbers increased during senescence, and immunolocalization experiments showed that they contained chloroplast stromal proteins, such as Rubisco and glutamine synthetase II, but not thy ...
PROTEINS IN NUCLEOCYTOPLASMIC
PROTEINS IN NUCLEOCYTOPLASMIC

... proportionality between the per cent of cytoplasm amputated during mitosis and the per cent of depletion of radioactive protein content of daughter nuclei, we predict that the daughter nuclei from cut mitotic cells will contain only half the protein of control nuclei, if exactly 50 % of the mitotic ...
Full Text - BioTechniques
Full Text - BioTechniques

... pin technologies, bubbles have been reported to create inconsistencies in the volumes printed, causing spot variations. In this study, the sizes of the spots printed by syringes varied from 400 to 600 µm. With these spot sizes, it was observed that the quantity and the quality of the printed spots w ...
Biosensor - PharmaStreet
Biosensor - PharmaStreet

... ,microorganisms, organelles,cell receptors , enzymes, antibodies, nucleic acids,etc.) or bio- mimetic component that interacts (binds or recognises) the analyte under study. ...
widespread phagocytosis of ciliates and other protists by marine
widespread phagocytosis of ciliates and other protists by marine

... also present, having either a round form with membrane-like invaginations in A. ostenfeldii and C. longipes (Figs. 16, 17) or the distinctive cap shape of starch plates in Amylax sp. (Figs. 18, 19). Both the cylindrical extrusome-like structures and starch plates resembled those routinely observed i ...
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Endomembrane system

The endomembrane system is composed of the different membranes that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes and the cell membrane. The system is defined more accurately as the set of membranes that form a single functional and developmental unit, either being connected directly, or exchanging material through vesicle transport. Importantly, the endomembrane system does not include the membranes of mitochondria or chloroplasts.The nuclear membrane contains two lipid bilayers that encompass the contents of the nucleus. The endoplasmic reticulum (ER) is a synthesis and transport organelle that branches into the cytoplasm in plant and animal cells. The Golgi apparatus is a series of multiple compartments where molecules are packaged for delivery to other cell components or for secretion from the cell. Vacuoles, which are found in both plant and animal cells (though much bigger in plant cells), are responsible for maintaining the shape and structure of the cell as well as storing waste products. A vesicle is a relatively small, membrane-enclosed sac that stores or transports substances. The cell membrane, is a protective barrier that regulates what enters and leaves the cell. There is also an organelle known as the Spitzenkörper that is only found in fungi, and is connected with hyphal tip growth.In prokaryotes endomembranes are rare, although in many photosynthetic bacteria the plasma membrane is highly folded and most of the cell cytoplasm is filled with layers of light-gathering membrane. These light-gathering membranes may even form enclosed structures called chlorosomes in green sulfur bacteria.The organelles of the endomembrane system are related through direct contact or by the transfer of membrane segments as vesicles. Despite these relationships, the various membranes are not identical in structure and function. The thickness, molecular composition, and metabolic behavior of a membrane are not fixed, they may be modified several times during the membrane's life. One unifying characteristic the membranes share is a lipid bilayer, with proteins attached to either side or traversing them.
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